• BMB Reports
• /
• 제54권10호
• /
• pp.528-533
• /
• 2021

Osteoarthritis (OA) is a degenerative disorder that can result in the loss of articular cartilage. No effective treatment against OA is currently available. Thus, interest in natural health products to relieve OA symptoms is increasing. However, their qualities such as efficacy, toxicity, and mechanism are poorly understood. In this study, we determined the efficacy of avenanthramide (Avn)-C extracted from oats as a promising candidate to prevent OA progression and its mechanism of action to prevent the expression of matrix-metalloproteinases (MMPs) in OA pathogenesis. Interleukin-1 beta (IL-1β), a proinflammatory cytokine as a main causing factor of cartilage destruction, was used to induce OA-like condition of chondrocytes in vitro. Avn-C restrained IL-1β-mediated expression and activity of MMPs, such as MMP-3, -12, and -13 in mouse articular chondrocytes. Moreover, Avn-C alleviated cartilage destruction in experimental OA mouse model induced by destabilization of the medial meniscus (DMM) surgery. However, Avn-C did not affect the expression of inflammatory mediators (Ptgs2 and Nos) or anabolic factors (Col2a1, Aggrecan, and Sox9), although expression levels of these genes were upregulated or downregulated by IL-1β, respectively. The inhibition of MMP expression by Avn-C in articular chondrocytes was mediated by p38 kinase and c-Jun N-terminal kinase (JNK) signaling, but not by ERK or NF-κB. Interestingly, Avn-C added with SB203580 and SP600125 as specific inhibitors of p38 kinase and JNK, respectively, enhanced its inhibitory effect on the expression of MMPs in IL-1β treated chondrocytes. Taken together, these results suggest that Avn-C is an effective candidate to prevent OA progression and a natural health product to relieve OA pathogenesis.

• 대한물리의학회지
• /
• 제9권2호
• /
• pp.151-159
• /
• 2014

PURPOSE: The purpose of this study was to evaluate whether light-emitting diodes (LED) irradiation could be effective in a noninvasive, therapeutic device for the treatment of osteoarthritis(OA). METHODS: Twenty-four male Sprague-Dawley rats were divided into four groups: Vehicle control (saline); monosodium iodoacetate-injection (MIA); LED irradiation after MIA injection (MIA-LED); indomethacin-treatment after MIA injection (MIA-IMT). OA was induced by intra-articular injection of 3 mg MIA through the patellar ligament of the right knee. Vehicle control rats were injected with an equivalent volume of saline. The LED was irradiated for 15 min/day for a week after 7 days of MIA treatment. To compare with the effect of LED irradiation, the indomethacin was administrated 20 mg/kg twice a week orally after 7 days of MIA treatment. Knee joints were removed and fixed overnight in 10% neutral buffered formalin and decalcified by EDTA for 2 week before being embedded in paraffin. The assessment of OA induction were monitored by knee movement and radiographic finding. Histologic analysis were performed following staining with hematoxylin and eosin, safranin O-fast green, or toluidine blue, picrosirius red, and histologic changes were scored according to a modified Mankin system. Apoptotic cell in tissue sections was detected using TUNEL method. RESULTS: Radiographic examination could not show the differences between the MIA-treated and the MIA-LED-treated rats. In the histologic analysis, however, LED irradiation prevented cartilage damage and subchondral bone destruction, and significantly reduced mononuclear inflammatory cell infiltration and pannus formation. LED irradiation also reduced apoptosis of cartilage cells, but it prevented apoptosis of infiltrated inflammatory cells in synovium. In addition, LED irradiation showed an increase of collagen production in the meniscus. CONCLUSION: These results suggest that the 840 nm LED irradiation would be a suitable non-thermal phototherapy for the treatment of OA, as a cartilage protection and anti-inflammatory modality.

• 대한관절경학회지
• /
• 제14권1호
• /
• pp.7-12
• /
• 2010

목적: 전방 십자 인대 재건술시 시행하는 반월상 연골판 전절제술 또는 아전절제술이 그 결과에 미치는 영향을 분석하고자 하였다. 대상 및 방법: 2003년 2월에서 2007년 2월까지 시행한 관절경적 전방 십자 인대 재건술를 시행한 455례 중 1년 이상 추시관찰이 가능하였던 93례를 대상으로 하였다. 전방 십자 인대 재건술을 단독 시행한 군은 45례, 반월상 연골판 전절제술 또는 아전절제술을 동시에 시행한 군은 48례였고, grade 3이상의 연골 손상, 반월상 연골판 봉합술과 부분 절제술을 동반한 경우는 제외하였다. 전방 십자 인대 재건술을 단독으로 시행한 군(I군)은 45명, 외측 반월상 연골 절제를 동시에 시행한 군(II군)은 10명, 내측 반월상 연골 절제를 동시에 시행한 군(III군)은 28명, 내측과 외측 반월상 연골을 동시에 절제한 경우(IV군)는 10명이었다. 임상적 평가로 IKDC 주관적 점수, Lysholm 점수를 비교하였고, 슬관절 관절운동 범위, 전방전위검사, Lachman 검사, Pivot shift 검사, KT-1000 관절계를 이용하여 슬관절의 기능을 평가하고 비교하였다. 결과: 최종 추시 시, IV군은 I군보다 IKDC 주관적 점수와 Lysholm 점수가 낮았으며, II군보다 IKDC 주관적 점수가 낮았다. KT-1000 관절계는 I군이 IV군보다 더 향상되었으며, 전방전위검사와 Lachman 검사는 I군이 III군보다 더 향상되었다. Pivot shift 검사에서는 모든 군에서 의미있는 차이를 나타내지 않았다. 결론: 내측 또는 내외측 반월상 연골판 절제술은 전방전위검사, Lachman 검사 KT-1000 관절계에서 불안정성과 관계가 있었으며, 내측과 외측 반월상 연골판 절제술은 반월상 연골판이 정상인 경우보다 더 낮은 임상적 점수와 관계가 있었다.