• Title/Summary/Keyword: melanoma cells

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Adenine Inhibits B16-F10 Melanoma Cell Proliferation

  • Silwal, Prashanta;Park, Seung-Kiel
    • Biomedical Science Letters
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    • v.26 no.3
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    • pp.179-185
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    • 2020
  • Adenine, a purine base, is a structural component of essential biomolecules such as nucleic acids and adenine nucleotides. Its physiological roles have been uncovered. Adenine suppresses IgE-mediated allergy and LPS-induced inflammation. Although adenine is known to inhibit lymphocyte proliferation, the effect of adenine to melamoma cells is not reported. Here, we investigated the growth inhibitory effects of adenine on B16-F10 mouse melanoma cells. Adenine suppressed the proliferation of B16-F10 cells in dose-dependent manner with the maximal inhibitory dose of 2 mM. Adenine treatment induced cell death molecular markers such as PARP and caspase 3 cleavages. Pan-caspase inhibitor z-VAD dramatically rescued the cell death molecular markers, cell proliferation recovered marginally. These results provide the possibility of adenine to be used as an anti-tumor agent.

Antioxidant enzyme activities and intracellular reactive oxygen intermediate (ROI) levels of flavonol quercetin in the presence of taurine on Bl6F10 murine melanoma cells.

  • Sim, Hue-Jeong;Kim, An-Keun
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2003.11a
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    • pp.66-66
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    • 2003
  • Flavonoids are phenolic compounds widely distributed in wide variety of edible plants including leafy vegetables, fruits, beverages. Quercetin is one of bioflavonoid compounds and has anti-tumor effect by suppressing tumor growth in vitro and in vivo, including multiple biological effects by antioxidant and effective anti-inflammatory agent. The present study investigated whether quercetin can enhance antioxidant enzyme activities (glutathione peroxidase: GPX, superoxide dismutase : SOD, catalase: CAT) and intracellular reactive oxygen intermediate (ROI) levels in the presence of taurine on B16F10 murine melanoma cells. From this result, the antioxidant enzyme activities of quercetin in the presence of taurine was enhanced. In addition, the same treatments decreased intracellular reactive oxygen intermediate levels on B16F10 murine melanoma cells. Taken together, these results demonstrate that the antioxidant effect of quercetin can enhance in the presence of taurine and it might play an important role in anti-tumor effect.

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The Effects of Areca catechu Extracts on Tyrosinase Gene Expression in B16 Mouse Melanoma Cells (B16 마우스 흑색 종양 세포에서 빈랑(Areca catechu) 추출물이 티로시나아제 유전자에 미치는 효과)

  • Chin, Jong-Eon;Cho, Nam-Chul
    • The Korean Journal of Food And Nutrition
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    • v.20 no.2
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    • pp.240-244
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    • 2007
  • In this study, we found that the methanolic extract of Areca catechu repressed expression of the tyrosinase gene in B16 mouse melanoma cells containing a tyrosinase promoter. Extract concentrations of 100 ${\mu}$g/ml and 500 ${\mu}$g/ml exhibited tyrosinase gene expression rates of aproximately 62% and 48%, respectively, compared to the control. The fraction layers consisting of ethyl acetate, butyl alcohol, and water showed repressive effects on the tyrosinase gene. In particular, the butyl alcohol fraction highly repressed at 100 ${\mu}$g/ml and 500 ${\mu}$g/ml. In the MTT assay, the methanolic extracts exhibited very low cytotoxicities at 1 ${\mu}$g/ml, 10 ${\mu}$g/ml, and 100 ${\mu}$g/ml.

Inhibitory Effects of Hydrolysable Tannins on Tyrosinase Activities in B16 Mouse Melanoma Cells

  • Cho, Soo-Min;Kwon, Young-Min;Lee, Jae-Hee;Lee, Min-Won
    • Natural Product Sciences
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    • v.8 no.4
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    • pp.183-185
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    • 2002
  • To investigate skin whitening natural substances, the effects on melanogenesis by measuring the tyrosinase activity and the melanin contents of three hydrolysable tannins, $1,2,6-tri-O-galloyl-{\beta}-D-glucose$ (1), 2,3-(S)-HHDP-D-glucose (2) and pedunculagin (3) in B16 melanoma cells were examined. $1,2,6-Tri-O-galloyl-{\beta}-D-glucose$ (1), 2,3-(S)-HHDP-D-glucose (2) and pedunculagin (3) inhibited tyrosinase activity in B16 melanoma cells in a dose-dependent manner.

Inhibitory effects of some medicinal plant extracts on the tyrosinase promoter activity on B16 mouse melanoma cells

  • Chin, Jong-Eon;Sun, Heung-Suk;Lee, Kwang-Jae;Choi, Tae-Jin;Ko, Yoo-Seung;Sohn, Hyun-Jung;Kim, Jeong-Joong;Jeon, Byung-Hoon;Blaise Lee, Hwang-Hee
    • Advances in Traditional Medicine
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    • v.1 no.2
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    • pp.6-13
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    • 2000
  • Melanin is specifically produced in melanocytes. The pathway for melanin biosynthesis is mainly controlled by tyrosinase. To estimate the inhibitory effect of melanin biosynthesis from 31 medicinal plants extracts, we tested the inhibitory effects of the tyrosinase promoter on B16 mouse melanoma cells. The result of this study demonstrated that Mori Radicis Cortex and Castena Fractus extracts only in tested medicinal plant extracts have high inhibitory effects on tyrosinase promoters with very low cytotoxicity on B16 mouse melanoma cells. Therefore, extracts of Mori Radicis Cortex and Castena Fractus were evaluated as very effective negative regulators of tyrosinase gene expression.

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Effect of Hot-water Extracts from Laminaria japonicus on Melanin Production in B16 Melanoma Cells (Bl6 Melanoma 세포에서 다시마 열수추출물이 멜라닌 생성에 미치는 영향)

  • Park , Yoon-Jong;Yoon, Mi-Yun;Lim, Hye-Won;Lee, Ji-Yun;Kim, Chang-Jong;Sim, Sang-Soo
    • YAKHAK HOEJI
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    • v.48 no.6
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    • pp.374-378
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    • 2004
  • To investigate the whitening activity of Laminaria japonicus, we measured the effect of hot-water extracts on melanin production in B16 melanoma cells. Melanocyte stimulating h ormone (MSH, $1{\mu}M$)-stimulated melanin production in B16 melanoma cells was significantly inhibited by fucoidan and hot-water extracts, but not by alginate. The purified tyrosinase activity was not affected by hot-water extracts, alginate and fucoidan. However, tyrosinase expression was significantly inhibited by fucoidan and hot-water extract in Western blot. These results suggest that inhibitory mechanism of hot-water extracts on melanin production may be due to the inhibition of tyrosinase expression but not to direct inhibition of tyrosinase, such an effect of which may be dependent on fucoidan.

Cytologic Features of Papanicolaou Smears of Malignant Melanoma Arising in Vagina - A Case Report - (질에서 발생한 악성 흑색종의 Papanicolaou 도말의 세포학적 소견 - 1례 보고 -)

  • Kang, Myung-Suk;Kim, Bohng-Hee;Park, Jae-Hoon;Kim, Youn-Wha;Park, Yong-Koo;Lee, Ju-Hie;Yang, Moon-Ho
    • The Korean Journal of Cytopathology
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    • v.6 no.2
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    • pp.209-213
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    • 1995
  • Primary malignant melanoma of vagina is a rare tumor which is easily misinterpretated in routine cytologic examination. We lately experienced a case of primary malignant melanoma of the vagina with direct cervical extension diagnosed by Pap smear. The cervicovaginal smear showed variable sized clusters of epithelial cells or singly scattered abnormal epithelial cells. Most of the tumor cells had round hyperchromatic nuclei with prominent nucleoli and brownish pigments in cytoplasm. The cytologic findings are compared with histologic features of resected specimen.

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An Ester Extract of Cochinchina Momordica Seeds Induces Differentiation of Melanoma B16 F1 Cells via MAPKs Signaling

  • Zhao, Lian-Mei;Han, Li-Na;Ren, Feng-Zhi;Chen, Shu-Hong;Liu, Li-Hua;Wang, Ming-Xia;Sang, Mei-Xiang;Shan, Bao-En
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.8
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    • pp.3795-3802
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    • 2012
  • Cochinchina momordica seeds (CMS) have been widely used due to antitumor activity by Mongolian tribes of China. However, the details of the underlying mechanisms remain unknown. In the present study, we found that an EtOAc (ethyl ester) extract of CMS (CMSEE) induced differentiation and caused growth inhibition of melanoma B16 F1 cells. CMSEE at the concentration of $5-200{\mu}g/ml$ exhibited strongest anti-proliferative effects on B16 F1 cells among other CMS fractions (water or petroleum ether). Moreover, CMSEE induced melanoma B16 F1 cell differentiation, characterized by dendrite-like outgrowth, increasing melanogenesis production, as well as enhancing tyrosinase activity. Western blot analysis showed that sustained phosphorylation of p38 MAP accompanied by decrease in ERK1/2 and JNK dephosphorylation were involved in CMSEE-induced B16 F1 cell differentiation. Notably, 6 compounds that were isolated and identified may be responsible for inducing differentiation of CMSEE. These results indicated that CMSEE contributes to the differentiation of B16 F1 cells through modulating MAPKs activity, which may throw some light on the development of potentially therapeutic strategies for melanoma treatment.

Experimental Study on the Effect of Houttuyniae Herbal Acupuncture on the Growth of Melanoma 816 in Mice (어성초(漁腥草) 약침(藥鍼)이 B16흑색종(黑色腫) 암(癌)모델에 대한 항종양효과(抗腫瘍效果) 및 면역반응(免疫反應)에 미치는 영향(影響))

  • Bae, Won-Young;Go, Hyung-Gyun;Kim, Chang-Hwan
    • Journal of Acupuncture Research
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    • v.18 no.1
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    • pp.186-201
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    • 2001
  • Purpose ; In order to study the effect of Houttuyniae herbal acupuncture on growth of melanoma B 16 and immunity in mice, the Control group with normal saline acupunchure after subcutaneous inoculation of B16BL6 cells, the Sample I with Houttuyniae herbal acupuncture manufactured by distilled water extraction method after subcutaneous inoculation of B16BL6 cells and the Sample II with Houttuyniae herbal acupuncture manufactured by alcohol extraction method after subcutaneous inoculation of B16BL6 cells were divided. Methods ; To evauluate the effect of Houttuynias herbal acupuncture on growth of melanoma B16 in mice, the weight of mouse melanoma, body weight, spleen index( $\sqrt(Weight of spleen/Body weight){\times}100$), lymphocytes numbers in mouse peripheral blood and spleen tissue, the percentage of CD4+ T cells, CD8+ and the ratio of CD4+/CD8+ in mouse peripheral blood and spleen tissue are measured. Results ; In study of the weight of mouse melanoma, Houttuyniae herbal acupuncture groups(Sample I, Sample II) showed statistically significant inhibitory effect. And in study of effect of reduction of change in body weight of mouse, Sample I showed statistically significant inhibitory effect, too. In study of reduction of spleen index, lymphocytes numbers in mouse spleen tissue, and the percentage of CD4+ and the ratio of CD4+/CD8+ T cells in mouse peripheral blood, Houttuyniae herbal acupuncture groups(Sample I, Sample II ) showed with statistically significant inhibitory effect. Also, Sample I showed inhibitory effect of reduction of the percentage of CD8+ T cells in mouse peripheral blood and spleen tissue and Sample II showed inhibitory effect of reduction of the percentage of CD4+ and the ratio of CD4+/CD8+ T cells in mouse spleen tissue with statistical significance. Conclusions ; The inhibitory effect of reduction of lymphocytes numbers in mouse peripheral blood and IL-2 productivity, Houttuyniae herbal acupuncture groups(Sample I, Sample II) didn't show significant effect.

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Anti-melanogenesis Effect of Phenolic Compounds Isolated from Gastrodia elata (천마(Gastrodia elate) 추출물로부터 분리된 페놀성 물질의 멜라닌 생성 억제작용)

  • 김경태;김진국;박선희;이정하;이수희;김기호;박수남
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.1
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    • pp.33-38
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    • 2004
  • Melanin pigmentation in human skin is a major defense mechanism against ultraviolet light of the sun, but abnormal pigmentation such as freckles, liver spot could be a serious aesthetic problem. Nearly all studies are mainly concentrated on searching for the materials that have inhibitory activities on tyrosinase. In this work, to isolate phenolic compounds from Gastrodia elata, we purified the extract through solvent fractionation, column chromatography, and recrystallization. They were identified as 4-hydroxybenzyl alcohol 1, bis(4-hydroxyphenyl)methane 2, gastrodin (4-${\beta}$-D-glucopyranosyloxybenzyl alcohol) 3 on the base of spectroscopic evidences. In order to investigate their depigmentation effect, inhibitory activity against mushroom tyrosinase and inhibitory activity of melanin synthesis in B16 melanoma cells were evaluated in vitro. We have found that 4-hydroxybenzyl alcohol 1 and gastrodin (4- ${\beta}$-D-glucopyranosyloxybenzyl alcohol) 3 have no tyrosinase inhibitory activity, but inhibit the melanin synthesis in B16 melanoma cells. Tyrosinase inhibitory activities of bis(4-hydroxyphenyl)methane 2 (IC$\_$50/ = 400 $\mu\textrm{g}$/mL) and butanol fraction (IC$\_$50/ = 46 $\mu\textrm{g}$/mL) were lower/higher than that of arbutin (IC$\_$50/ = 114 $\mu\textrm{g}$/mL), but inhibitory activities of melanin synthesis in B16 melanoma cells were much higher than that of arbutin. Especially, tyrosinase inhibitory activities of isolated phenolic fraction (IC$\_$50/ = 2.37 $\mu\textrm{g}$/mL) from butanol fraction was very higher than that of arbutin (IC$\_$50/ = 114 $\mu\textrm{g}$/mL). Therefore, these results suggest that isolated phenolic compounds from Gastrodia elata have inhibitory activity against mushroom tyrosinase and inhibitory activity of melanin synthesis in 816 melanoma cells in vitro.