• Journal of Applied Biological Chemistry
• /
• 제57권4호
• /
• pp.347-352
• /
• 2014

Pueraria mirifica 뿌리를 실온에서 70% EtOH 수용액으로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었다. EtOAc 분획에 대하여 silica gel, octadecyl silica, 및 Sephadex LH-20 c.c.를 반복 실시하여 4종의 화합물을 분리, 정제하였다. Nuclear magnetic resonance, infrare, 및 mass spectrometry의 spectroscopic data를 해석하여, 화합물 1-4를 각각 megastigm-5-en-3,9-diol, linarionoside B, 3,5,6,9-tetrahydroxy-megastigm-7-ene 및 3,4,9-trihydroxymegastigma-5,7-diene으로 구조를 결정하였다. 화합물 1-4 모두 P. mirifica에서는 이번에 처음으로 분리된 화합물이다.

• Natural Product Sciences
• /
• 제26권2호
• /
• pp.151-157
• /
• 2020

Extensive column chromatography separation of the MeOH extract from the aerial parts of Phyllanthus urinaria afforded seventeen compounds (1 - 17). The structures of the compounds were elucidated by physicochemical and spectroscopic methods to be 5'-β-D-glucopyranosyloxyjasmonic butyl ester (1), (+)-cucurbic acid (2), dendranthemoside B (3), boscialin 4'-O-β-D-glucoside (4), 4,5-dihydroblumenol A (5), (6R,9R)-megastigman-4-ene-9,13-diol (6), (3S,5R,6S,9R)-3,6-dihydroxy-5,6-dihydro-β-ionol (7), (6S,9R)-roseoside (8), mallophenol B (9), icariside B₅ (10), corchoinoside B (11), canangaionoside (12), 5,6-epoxy-3-hydroxy-7-megastigmen-9-one (13), icariside B₂ (14), (7E)-2β,3β-dihydroxy-megastigm-7-en-9-one (15), betulalbuside A (16), and loliolide (17). The compounds 1, and 3 - 16 were isolated for the first time from this plant. The absolute stereochemistry of compound 1 was newly determined. The isolated compounds were tested for cytotoxic activity against four human tumor cell lines in vitro using a Sulforhodamin B bioassay, but all the compounds showed weak cytotoxic activities.