• 제목/요약/키워드: medium condition

검색결과 2,064건 처리시간 0.025초

잎새버섯 균주의 균사체 생육 최적조건 (Optimal Condition of Mycelial Growth of Grifola frondosa)

  • 지정현;김정한;원선이;서건식;주영철
    • 한국균학회지
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    • 제35권2호
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    • pp.76-80
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    • 2007
  • 잎새버섯의 우량계통을 선발하여 농가에 보급하기 위하여 수집균주들의 균사체 생육 최적조건을 검토한 결과는 다음과 같다. 잎새버섯 수집균주의 생장에 적합한 배지는 PDA배지가 선발되었으며, 균사배양 적온은 $25^{\circ}C$로 나타났다. 잎새버섯의 균사생장에 적합한 수소이온 농도는 pH $4{\sim}5$로 약산성에서 균사생장이 우수하였다. 균사생장에 적합한 탄소원으로는 glucose와 fructose, 질소원으로는 peptone이 우수하였으며 C/N율은 $10{\sim}20$으로 질소 요구도가 다소 높은 것으로 나타났다.

치과위생사 유니폼 착용실태, 만족도, 디자인 평가에 관한 연구 (The Wearing Condition, Satisfaction, and Design Evaluation of Dental Hygienists' Uniform)

  • 황은경;정성지;장남경
    • 복식문화연구
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    • 제14권1호
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    • pp.93-107
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    • 2006
  • This study was designed from the concept that there is a need to establish the uniform for new emerging jobs in modern society. The purpose of the study was to examine dental hygienists'wearing condition, satisfaction, and design evaluation as to provide information for the development of dental hygienists' uniform. Three hundred fifteen dental hygienists completed questionnaires, and data were analyzed by descriptive statistics, factor analysis, multiple regression, ANOVA, and Duncan's test. The findings from the study were following. First, majority of dental hygienists currently wear shirts and pants two-piece uniform in white with no print. Second, dental hygienists' overall satisfaction level for their uniform was medium, and symbolic satisfaction as well as functional satisfaction did not reach the medium level while aesthetic satisfaction was above medium. The satisfaction levels were significant different between groups according to the education, clinic size, and work experience. Third, aesthetic satisfaction levels influenced on the job image as professional. Finally, designs consisting of two-piece style with shirts and pants and having Peter pan collar were recorded high evaluation. The findings can be implemented into dental hygienists' uniform design.

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Optimal Culture Conditions for the Shoot Growth of Freesia 'Shiny Gold' during Bioreactor Culture

  • Kang, Ji Su;Jeong, Kyeong Jin;Choi, Youn Jung;Yun, Jae Gill
    • 한국자원식물학회지
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    • 제30권6호
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    • pp.699-706
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    • 2017
  • For rapid production of freesia 'Shiny Gold' shoots by using a bioreactor, several culture conditions were investigated. Young shoots (< 1 cm) obtained from freesia corm section in vitro were used as plant materials for this experiment. As a basic experimental environment, 20 young shoots were inoculated into a 5 L balloon type bubble reactor which contained 1 L 1/2 strength MS medium supplemented with 30 g sucrose (3%), and the aeration was 0.1 vvm (vessel volumes per minute). The bioreactors were placed in a growth room with $23^{\circ}C$ temperature, 60% relative humidity and $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ light condition (16 h/8 h, day/night). The concentrations of MS media were set with 1/4, 1/2, 1 strength, medium volume 10, 20, 40%, sucrose concentration 3, 6, 9%, and aeration 0.1, 0.2, 0.4 vvm. After 4 weeks of cultivation, the growth indexes including the fresh and dry weight, and plant height were evaluated. At the same time, the consumption, pH, and EC of medium were estimated 4 weeks after incubating. The best results were achieved when 40 young shoots were incubated in a bioreactor in which 1 L of 1/2 strength MS medium supplemented with 6% sucrose was used for the rapid production of freesia shoots. The shoots were 17 cm in plant height and 1.0 g in fresh weight only 4 weeks after incubation which could be a good plant material suitable for corm enlargement in vitro. No correlation was observed between the growth of freesia shoots and the consumption, pH or EC of medium.

The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots

  • Shim, Jae-Ouk;Chang, Kwang-Choon;Lee, Youn-Su;Park, Cheol-Ho;Kim, Hey-Young;Lee, U-Youn;Lee, Tae-Soo;Lee, Min-Woong
    • Mycobiology
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    • 제34권4호
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    • pp.206-208
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    • 2006
  • To produce an artificial fruiting body of Armillaria mellea on the oak sawdust medium, seven strains of A. mellea were used. The top surface of oak sawdust medium covered with ground raw carrot was inoculated with each of 7 strains and cultured for 30 days at $25^{\circ}C$ in the dark condition until the mycelia of A. mellea completely colonized the medium from top to bottom. Then, the mycelia which were fully covered on the top surface of the medium were scratched slightly with a spatula and filled with tap water for 3 hours. To induce the primordial formation, the 7 strains of A. mellea were transferred to the growth chamber under the illumination (350 lux) of 12 hours and relative humidity of $85{\pm}5%$ in a day and then cultured at $16{\pm}1^{\circ}C$. Only A. mellea IUM 949 could form primordia on the sawdust medium, but the other strains did not make primordia at the same condition. The primordia of A. mellea IUM 949 were formed 10 days after complete colonization of the medium and the fruiting bodies were produced 7 days after a primordial formation. The experimental results suggested that IUM 949 strain might be a good candidate for mass production of fruiting bodies of A. mellea.

Hohenbuehelia petaloides의 배양학적 특성에 관한 연구 (Studies on the Cultural Characteristics of Hohenbuehelia petaloides)

  • 유관희;김준호;석순자
    • 한국균학회지
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    • 제29권1호
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    • pp.52-60
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    • 2001
  • This study was carried out to obtain the basic data on artificial culture of Hohenbuehelia petaloides. The optimum medium are glucose peptone medium (GP), Hennerberg medium, Phellinus igniarius medium (PIM), Lentinus edodes medium (LEM), Czapek dox medium. The optimum condition for the mycelial growth was $30^{\circ}C$ and pH 6.0. The carbon sources such as dextrine, fructose and lactose were favorable to mycelial growth. The optimal concentrations of carbon sources are 10% dextrin and fructose. As nitrogen sources, tryptone, casamino acid and histidine appeared to be favorable. The optimal concentrations of nitrogen sources are 1% soy tone and 0.3% ammonium nitrate. The optimal concentration of yeast extract is 0.4%. The mineral nutrients of $KH_2PO_4$, $K_2HPO_4\;and\;MgSO_4{\cdot}7H_2O$ were effective and the optimal concentrations were 0.046, 0.1 and 0.05%, respectively.

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중형 상용차량 ESC 평가를 위한 Sine with Dwell Test 제안 (Proposal for Using Sine with Dwell for the Evaluation of ESC for Medium Commercial Vehicles)

  • 권백순;이경수
    • 자동차안전학회지
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    • 제7권2호
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    • pp.32-38
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    • 2015
  • A sine with dwell test is well known as a test scenario for evaluation of performance of electronic stability control(ESC) on passenger vehicles and heavy commercial vehicles. However, when it comes to ESC for medium commercial vehicles, the test scenario has not been established yet. In this paper, the sine with dwell test was modified considering characteristics of medium commercial vehicles. The three main modifications of the original test scenario are the steering angle level, steering frequency, and loading condition of the vehicle. These modifications are derived from simulation study for different medium commercial vehicles. From simulation study, it was shown that the ESC system for medium commercial vehicle is objectively evaluated by the proposed test scenario. A clear improvement on vehicle stability was seen in the results when ESC system was used.

안전 교육 실태 조사를 통한 중소기업의 안전 교육 모델 개발 (- An Education Model Improvement of the Small and Medium Sized Enterprises for Survey of Safety Education Actual Condition -)

  • 전미경;홍성만;박범
    • 대한안전경영과학회:학술대회논문집
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    • 대한안전경영과학회 2003년도 추계학술대회
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    • pp.1-13
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    • 2003
  • Domestic industry calamity rate did decrease in large numbers by many efforts of the safety and the health organizations. Nevertheless domestic industrial accident level is low yet among the OECD countries. Domestic small and medium enterprises is in trend that industrial accident is increased by deterioration of hardware, unpreparedness of safety equipments. But, Domestic small and medium enterprises' safety and health education had not been utilized so far effectively. This research groped efficient operation model of the safety and the health education through the survey about a solution for problems of education that is enforced in small and medium enterprises to three groups of workers and safety and health administrators and managers. Compared domestic safety and health educational system and advanced foreign educational system. And, analyze problems about domestic small and medium enterprise's safety and health education and presented a solution. Finally, presented efficient operation model of the safety and the health education suitable to domestic medium and small enterprises.

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실측 파형과 수치 파형에 의한 진동주응력 비교 (Comparison of the Vibration Principal Stress by Experimental and Numerical Waveform)

  • 홍웅기;송정언;박영민
    • 환경영향평가
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    • 제21권5호
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    • pp.609-615
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    • 2012
  • In recent years, the development of computer technique was possible to the simulation analysis of the structure caused by ground vibration. Generally, finite element method(FEM) has been used in these structural analysis. In this study, it was calculated to the vibration energy as measuring vibration waveform, and estimated about principal stress due to medium characteristics of the ground as processing dynamic analysis by the vibration energy. The results are as follows : Firstly, the principal stress distribution in all mediums was different due to a medium condition, and the principal stress at concrete medium was represented to difference due to physical characteristics. Secondly, the principal stress by time increasing was represented to maximum amplitude within 0.03 second. And also, the principal stress after maximum amplitude was very large at concrete medium, which was considered to be formed compression or tension range at a medium boundary. Thirdly, the variation of principal stress at concrete medium was represented in the order of RC medium, NC=H medium, NC=S medium. It was considered that the vibration energy propagated fast when a medium have a big elasticity and density.

더덕의 花粉發芽에 관한 溫度와 培地條件 (Factors Influencing Pollen Germination in vitro of Codonopsis lanceolata)

  • 김학현
    • 한국자원식물학회지
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    • 제11권1호
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    • pp.106-110
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    • 1998
  • The present study has been undertake to obtain the fundatmental data of optimum germination condition and to establish storage time for artificial pollinationin Codonopsis lancelata pollen. In vitro condition for germination of freshly collected and stored pollen were examined. The optium temperature for germination of fresh pollen was $25^{\circ}C$. The optium sucrose concentration in the medium ranged from 30 to 40 % and optium pH 6.0% for pollen germination. The rate of pollen germination accelerated considerably in the medium with 1% agar. 30% sucrose, and pH 6. C. lanceolata pollen remained viable for 15 days when stored at 5$^{\circ}C$ with silica gel as desiccant.

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중소규모 제조업 근로자의 직무스트레스 분석 (A Study on the Job Stress of Small and Medium Sized Industry Workers)

  • 윤순녕;전경자;김춘미
    • 한국직업건강간호학회지
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    • 제6권2호
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    • pp.79-95
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    • 1997
  • The purposes of this study were to develop a job stress measurement tool and to identify the job stress according to the characteristics of worker and work condition at the small and medium-sized plants. Data were collected through face-to-face survey for April-May, 1997. Subjects were 180 workers at 36 small and medium-sized plants located near Seoul. Data were analyzed by item analysis, factor analysis, and ANOVA. The results were as follow : 1, Number of items of job stress measurement were 33 and Cronbach's $\alpha$ was .9262 except 13 items with low values of Cronbach's coefficient. Factor analysis was done in order for construct validity and 8 factors were extracted and cumulative percent of variance was 65.6%. 2. The job stress scores of male, the younger, 2-shift workers, and over-time workers were significantly higher than those of female, the older, 1-shift workers, and 48 hour workers per week. 3. There were no significant difference between job stress scores and the groups by number of worker, and the types of manufacturing. The stress measurement tool is valid and very reliable to measure worker's stress. Furthermore, the job stress managent program for health promotion of the workers at the vulnerable work condition is needed more and more.

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