• 제목/요약/키워드: medium composition.

검색결과 988건 처리시간 0.029초

Effect of Unsaturated Fatty Acids on Cellulose Degradation and Fermentation Characteristics by Mixed Ruminal Microbes

  • Hwang, I.H.;Kim, H.D.;Shim, S.S.;Lee, Sang S.;Ha, J.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권4호
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    • pp.501-506
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    • 2001
  • This experiment was conducted to evaluate the effects of supplemental unsaturated fatty acids (UFA) on fermentation characteristics, especially on gas production, cellulose degradation and volatile fatty acid (VFA) concentration by mixed ruminal microorganisms. In order to attain this objective, unsaturated fatty acids including oleic acid (C 18:1), linoleic acid (C18:2) and arachidonic acid (C22:4) were added at varying level. Mixed ruminal microbes used in this experiment were obtained from the rumen of a cannulated Holstein cow. Medium pH values after 7 d incubation were significantly affected by type and level of unsaturated fatty acids (p<0.01). All of UFA inhibited total gas production, and especially treatment of arachidonic acid at the levels of 0.01% gave the lowest gas. production after 7 d incubation (p<0.01). Comparison of the population of protozoa revealed that UFA did not have any significant effect on the total protozoa number. The addition of UFA did not effect dry matter degradation. Volatile fatty acid (VFA) composition of the culture was influenced little by UFA, although the considerable amount of iso-type VFA were detected in UFA supplemented incubations. The ratio of acetic acids to propionic acids, however, was lower than control in all the treatments after 7 d incubation (p<0.01).

Brevibacterium sp. 로부터 유도된 PFP 내성 변이주에 의한 L-Tyrosine 생성 (Production of L-Tyrosine by PFP Resistant Mutant Induced from Brevibnrcterium sp.)

  • 배준태;박경숙;이별나
    • 한국식품영양학회지
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    • 제9권1호
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    • pp.21-28
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    • 1996
  • This study was attempted to investigate the production of L-tyrosine by Brevibacterium flavum ATCC 14067. To select the strain which produce more L-tyrosine, mutants were induced by N-methyl-N'-nitro-nitrosoguanidine (NTG) treatment and phenylalanine auxotrophic mutants were induced by NTG and penicillin treatments. PFP resistant mutant was isolated from a phenylalanine auxotroph by retreatment with NTG and screened for increase of L-tyrosine production. PFP-326 mutant resistant to PPP (100ug/ml) was derived from phenylalanine auxotroph by mutagenesis with NTG and PFP-106 mutant resistant to PFP (1201g/ml) was derived from PFP-326 by mutagenesis with NTG. The composition of media for L-tyrosine production in strain PFP-106 was studied. PFP-106 mutant strain produced 50mg 11 of L-tyrosine while the parent strain produced 0.56mg 11 of L-tyrosine. The optimum composition of medium for L-tyrosine by strain PFP-106 was 10cA sucrose as carbon source, 3% ammonium sulfate as nitrogen source. The optimum cultural condition for producing L-tyrosine by strain PFP-106 was L-phenylalanine at a concentration of 1000g/mg.

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Mcl-PHAs Produced by Pseudomonas sp. Gl01 Using Fed-Batch Cultivation with Waste Rapeseed Oil as Carbon Source

  • Mozejko, Justyna;Wilke, Andreas;Przybylek, Grzegorz;Ciesielski, Slawomir
    • Journal of Microbiology and Biotechnology
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    • 제22권3호
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    • pp.371-377
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    • 2012
  • The present study describes medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production by the Pseudomonas Gl01 strain isolated from mixed microbial communities utilized for PHAs synthesis. A two-step fed-batch fermentation was conducted with glucose and waste rapeseed oil as the main carbon source for obtaining cell growth and mcl-PHAs accumulation, respectively. The results show that the Pseudomonas Gl01 strain is capable of growing and accumulating mcl-PHAs using a waste oily carbon source. The biomass value reached 3.0 g/l of CDW with 20% of PHAs content within 48 h of cultivation. The polymer was purified from lyophilized cells and analyzed by gas chromatography (GC). The results revealed that the monomeric composition of the obtained polyesters depended on the available substrate. When glucose was used in the growth phase, 3-hydroxyundecanoate and 3-hydroxydodecanoate were found in the polymer composition, whereas in the PHAs-accumulating stage, the Pseudomonas Gl01 strain synthesized mcl-PHAs consisting mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate. The transcriptional analysis using reverse-transcription real-time PCR reaction revealed that the phaC1 gene could be transcribed simultaneously to the phaZ gene.

Morphological Characteristics and Karyotypic Analysis of Aster spathulifolius According to Native Area

  • Yoon Pyung-Sub;Park Hye-Mi;Kim Dong-Min;Kim Hyun-Hee
    • Plant Resources
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    • 제8권3호
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    • pp.244-249
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    • 2005
  • The growth charateristics and karyotypes of Aster spathulifolius collected from 5 sites including coastal and island region on the Korean peninsula, were analysed. Several morphological characteristics of the plants such as leaf length, leaf width, top internode, medium internode, spike branching, flower diameter, number of petal, leaf color, leaf form, stem and leaf hair, viscosity, and serration of the plants were distinctly different depending on the native region from which they were collected. Karyotypic analysis showed that the chromosome number was all diploid (2n=18), with one pair of submetacentric satellite chromosomes. The chromosome composition included 7 pairs of metacentric chromosomes and 2 pairs of submetacentric chromosomes in all plants. However, chromosome order and the ranges of the chromosome lengths were a little different from plant to plant according to their native growing regions. The plants from Geoje-Do especially showed large differences in the chromosome lengths between the longest and the shortest compared to the plants from other places. This results provide important data to support the classification of the species into several sub-species.

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LABORATORY SIMULATION OF LIGHT SCATTERING FROM REGOLITH ANALOGUES: EFFECT OF POROSITY

  • KAR, AMRITAKSHA;DEB, SANJIB;SEN, A.K.;GUPTA, RANJAN
    • 천문학논총
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    • 제30권2호
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    • pp.65-67
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    • 2015
  • The surfaces of most atmosphereless solar system objects are referred to as regolith, layers of loosely connected fragmentary debris, produced by meteorite impacts. Measurements of light scattered from such surfaces provides information about the composition and structure of the surface. A suitable way to characterize the scattering properties is to consider how the intensity and polarization of scattered light depends on the particle size, composition, porosity, roughness, wavelength of incident light and the geometry of observation. In the present work, the effect of porosity on bidirectional reflectance as a function of phase angle is studied for alumina powder with grain size of $0.3{\mu}m$ and olivine powder with grain size of $49{\mu}m$ at 543.5 nm. The optical constants of the alumina sample for each porosity were calculated with Maxwell Garnett effective medium theory. On using each of the optical constants of alumina sample in Mie theory with the Hapke model the variation of bidirectional reflectance is obtained as a function of phase angle with porosity as a parameter. Experimental reflectance data are in good agreement the model. For the olivine sample the effect of porosity is studied using Hapke (2008).

미술관 전시공간의 시각구조 분석 - 개실형 전시공간의 분석을 중심으로 - (An Analysis on The Visual Configuration of an Exhibition Space in Art Museum - Focusing on an Exhibition space Room-to-room -)

  • 이종숙;임채진
    • 한국실내디자인학회논문집
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    • 제16권6호
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    • pp.197-204
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    • 2007
  • In the spatial configuration of room-to-room art museums, openings and walls are important physical factors, and openings are a medium for connecting spaces. Also, by spatially composing walls and openings, spaces can be connected visually and physically, and movements and directions may vary depending on the location or size of walls and openings. In this study, 5 museums are investigated: Sainsbury Wing in London, Gemaldegalerie In Berlin, Alte Pinakotkek in Munich, Neue Pinakothek in Munich, and Staatsgalerie Stuttgart in Stuttgart. Using DepthMap program, the characteristics of visual-spatial composition of each museum are derived by comparing the Connectivity, Visual integration, and Isovist maximum radial. From an analysis of spatial distribution, a relationship with visibility is investigated. The results of this study showed that the combination of visual axial lines affect the spatial configuration of the room-to-room museums. Depending on the location of opening, the museums can be classified as follows: 1) Museums with the 1st visual axial line and the 2nd and 3rd auxiliary visual axial lines ; 2) museums with several equal visual axial lines; and 3) museums with only center axial line.

PMC(Programmable Metallization Cell) 응용을 위한 칼코게나이드 박막에서의 Ag-doping에 관한 연구 (A Study on Ag-doping in Chalocogenide Thin Films Application for Programmable Metallization Cell)

  • 최혁;남기현;구용운;이영희;정홍배
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2007년도 제38회 하계학술대회
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    • pp.1325-1326
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    • 2007
  • We have demonstrated new functionalities of Ag doped chalcogenide glasses based on their capabilities as solid electrolytes. Formation of such amorphous systems by the introduction of silver via photo-induced diffusion in thin chalcogenide films is considered. The influence of silver on the properties of the newly formed materials is regarded in terms of diffusion kinetics and Ag saturation is related to the composition of the hosting material. Silver saturated is related to the composition of the hosting material. Silver saturated Ge-Ch glasses have been used in the formation of solid electrolyte which is the active medium in programmable metallization cell (PMC) devices. This paper concerns our more recent work on silver-doped germanium selenide electrolytes and describes the electrical characteristics of PMC devices made from these materials following annealing at $300^{\circ}C$.

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Synthesis and Characterization of Copoly(amide-imide) Derivatives and Ultrafiltration Membrane Performances I - Preparation of Copoly(amide-imide)s by One-step Method -

  • Jeon, Jong-young;Shin, Bong-Seob
    • Korean Membrane Journal
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    • 제3권1호
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    • pp.9-16
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    • 2001
  • The diamide-diamine having carboxylic acid was prepared by direct condensation of 1,2,4-benzenetricarboxylic acid with bits[4- (3-aminophenoxy ) phenyl] sulfone and bits(4-aminouhenyl)-1,4- diisopropylbenzene in medium consisting of triphenylphosphite, LiCl, and N-methyl-2-pyrrolidone. Copoly (amide-imide) derivatives with high molecular weight could be synthesized by one-step polycondensation of prepared diamide-diamine having carboxylic acid and various dianhydride compounds. Depending on the chemical structure and composition of polymer backbones, the viscosities of polymers were found to range between 0.87∼ 1.57 dL/B. All the polymers showed good thermal stability up to 320$\^{C}$ and the 10% weight loss temperature was observed in the range of 450∼540$\^{C}$ in a thermogravimetric traces. The glass transition was recorded in the temperature range of 200 ∼ 270$\^{C}$. All the polymers showed an amorphous nature on a differential scanning calorimetric thermograms. These polymers generally had good mechanical properties and readily soluble in various polar solvents. Further, it was proved that their properties could be determined from the composition.

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배트염료에 의한 나일론과 폴리에스테르 섬유의 염색성 - 색상 변화에 미치는 하이드로슬파이트와 NaOH의 영향 - (Dyeing Properties of Nylon 6 and Polyester Fabrics with Vat Dyes - Effect of Composition of Reducing Agent and Alkali on Color Change -)

  • 정동석;이두환;이문철
    • 한국염색가공학회지
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    • 제14권5호
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    • pp.284-293
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    • 2002
  • Nylon 6 and polyester taffeta fabrics are dyed in aqueous medium with vat dyes such as Indanthren Red FBB, Mikethren Blue ACE and Mikethren Blue HR varying the compositions of sodium hydrosulfite and NaOH. Also nylon UMF nonwoven and polyester UMF knitted fabrics are dyed with metal complex and disperse dyes as a reference, and the wash and rubbing fastnesses for these dyes are investigated. In vat dyeing of polyester and nylon taffeta, an optimum composition of sodium hydrosulfite/NaOH is existed at a range of 1∼2wt%/0.2wt%. A good build-up property for Mikethren Blue ACE on nylon 6 UMF nonwoven fabric is shown at high temperature. Vat dyeing of polyester with Mikethren Blue Ace shows a good color shade in a higher temperature, while dyeing with Mitsui Blue HR shows low temperatures. Vat dyes In dyeing of both nylon 6 UMF nonwoven and polyester UMF knitted fabrics have a better wash fastnesses compared with metal complex or disperse dyes.

수유 기간에 따른 모유의 총지질, 총콜레스테롤 및 비타민 E 함량과 총지방산 조성의 변화에 관한 연구 (A Longitudinal Study of the Total Lipid, Total Cholesterol, and Vitamin E Contents and Fatty Acids Composition of Human Milk)

  • 문수재
    • Journal of Nutrition and Health
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    • 제26권6호
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    • pp.758-771
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    • 1993
  • This study was conducted longitudinally following the total lipid and cholesterol content, fatty acids composition and Vitamin E content in human milk from 2-5 days to 12 weeks postpartum of 45 lactating Korean mothers. All samples were from well-defined subjects and uniform collection procedures were used. Total lipid concentration increased from 1.98g/dl at 2-5 days to 3.09g/dl at 12 weeks postpartum. However, there was a large variation in the concentration of total lipids. Total cholesterol concentration tended to decrease from 20.68mg/dl at 2-5days to 17.19mg/dl at 12 weeks. In comparison with transitional and mature milk, human colostrum was characterized by a lower percentage of saturated fatty acids including medium chain length fatty acids and a higher percentage of monounsatureated fatty acids and polyunsaturated fatty acids. Therefore, the P/S and M/S ratios of human milk lipids decreased significanlty during lactation. P/M/S ratio of colostrum changed from 0.38 : 0.84 : 1.00 at 2-5 days to 0.29 : 0.61 : 1.00 at 12 weeks of mature milk. Total tocopherol concentration decreased significantly from 833 ug/dl at 2-5 days to 300 ug/dl at 12 weeks.

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