• Microbiology and Biotechnology Letters
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• v.7 no.2
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• pp.65-70
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• 1979

By the successive enrichment culture, methanol-utilizing bacteria of 213 strains were isolated from soil samples collected from various places. Among them one strain showing excellent growth was selected. The organism isolated was obligate methylotroph and identified as Methylomonas methanolica on the basis of its mophological and physiological characteristics of the cell. The medium have been to be collected for the maximum biomass productivity. The microorganism was capable of growing satisfactorily on a medium containing only methanol 0.8％ (v/v), ammonium sulfate 0.6％, magnesium sulfate 0.1％, phosphate salts, but did not require growth factor.

• Journal of the Korean Wood Science and Technology
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• v.44 no.2
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• pp.231-240
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• 2016

The change in chemical compositions of leachate and medium density fiberboard (MDF) from a laboratory-scale simulated landfill which constructed in a plastic container containing alternating layers of soil and MDF was investigated to evaluate decomposing of MDF in soil. Four treatments were conducted: 1) MDF in soil, 2) MDF only, 3) cured UF resin in soil, and 4) soil only. Molecular weight (MW) distribution of compounds in leachate from soil only treatment did not change over time. In UF resin in soil treatment, the MW distribution shifted to a lower MW distribution over time, while the peak shifted to the left indicated changing to higher MW distribution in leachate from treatment 1 and 2 contained MDF. Higher percent nitrogen in leachate was observed in MDF containing treatments due to the UF resin in the MDF. The percent carbon slightly increased in MDF only while that greatly decreased in MDF in soil treatment maybe due to bacterial activity. The percent of extractable materials from the MDF decreased greatly on day 35 compare to day 0, and subsequently did not change much on day 77. In contrast, percent holocellulose and lignin did not change much over time. No structural change of the wood fiber in MDF occurs during the study. Water-soluble materials from MDF in soil contributed the change in chemical composition of leachate.

• The Journal of Sustainable Design and Educational Environment Research
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• v.2 no.1
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• pp.49-67
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• 2002

The purpose of this study is to be able to accept various educational processes with a few classrooms by multi-purpose uses special classrooms and supporting facilities of medium and small sized elementary school. For this purpose, I had divided into three parts by analyzing correlation of each classrooms according to the types of lessons, necessary equipment, building equipment environment, the need of users. The types of lessons and necessary facilities make the scope of each classrooms throughout Correspondence Analysis, and the need of users that is the recognized similarity between each rooms by teachers, make the scope by using Multi-Dimensional Scaling(MDS). Establish the final scope by combining and analyzing the already classified rooms by each factor. Lastly, calculate the numbers of rooms by applying the usage rate of each classified classroom and examine the standard of planning and the example of composition for the multi-purpose uses of combined room.

• Geomechanics and Engineering
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• v.14 no.1
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• pp.19-27
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• 2018

It follows from the basic principles of mechanics of deformable solids relating to the strength, stability and propagation of elastic waves that the Earth's inner core cannot exist in the form of a spherical structure in the assumed thermobaric conditions and calculation values of physico-mechanical parameters. Pressure level reaches a value that is significantly greater than the theoretical limit of medium strength in the model approximations at the surface of the sphere of the inner core. On the other hand, equilibrium state of the sphere is unstable on the geometric forming at much lower loads under the influence of the "dead" surface loads. In case of the action of "follower" loads, the assumed pressure value on the surface of the sphere is comparable with the value of the critical load of "internal" instability. In these cases, due to the instability of the equilibrium state, propagation of homogeneous deformations becomes uneven in the sphere. Moreover, the elastic waves with actual velocity cannot propagate in such conditions in solid medium. Violation of these fundamental conditions of mechanics required in determining the physical and mechanical properties of the medium should be taken into account in the integrated interpretations of seismic and laboratory (experimental) data. In this case, application of the linear theory of elasticity and elastic waves does not ensure the reliability of results on the structure and composition of the Earth's core despite compliance with the required integral conditions on the mass, moment of inertia and natural oscillations of the Earth.

• Fisheries and Aquatic Sciences
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• v.11 no.3
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• pp.149-158
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• 2008

Labyrinthulids are unicellular heterotrophic marine microalgae. Two labyrinthulid strains, L4 and L75, which produce generous amounts of polyunsaturated fatty acids(PUFAs), were isolated from leaves floating in the coastal areas of Quang Ninh province, Vietnam, and Fukuoka Prefecture, Japan, respectively. Both strains had spindle-shaped cells surrounded by ectoplasmic networks. Numerous oil bodies were observed in each cell, mostly in the vicinity of cell membranes. When soybean oil or soybean lecithin was used as a carbon source with peptone as a nitrogen source, the proportion of PUFAs reached 25-30% and 50-56% for L4 and L75, respectively. After 14 days of growth at $25^{\circ}C$, L4 produced 0.3 mg PUFAs/g-agar in PYA-SBO medium and 0.6 mg PUFAs/g-agar in PYA-SBL medium. In comparison, L75 produced 0.2 mg PUFAs/g-agar in both types of media. The differences between the two strains included changes in cellular morphology and the capacity for attaching tightly to fibers when cultured in liquid PYA medium containing 2% SBL. In addition, when the strains were grown under the same conditions, L4 had a higher growth rate and produced more PUFAs than L75.

• Microbiology and Biotechnology Letters
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• v.32 no.4
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• pp.366-370
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• 2004

Four chitinase producing bacteria, Arthrobacter nicotinae CH4, Arthrobacter nicotinae CHI3, Arthrobacter sp. CH5 and Micrococcus sp. CH3, were isolated from small crabs and shrimps. We investigated the optimum medium condition for the production of enzyme and high cell mass. The preferable medium composition was as follows: colchitin 0.1 %(w/v), glycerol 0.25%(w/v) and yeast extract 0.05%(w/v) in minimal midium ($K_{2}HPO_{4}$ 0.7 g/l, $KH_{2}PO_{4}$ 0.3 g/l, $MgSO_{4}{\cdot}5H_{2}O$ 0.5 g/l, $FeSO_{4}}{\cdot}7H_{2}O$ 0.01 g/l, $ZnSO_{4}$ 0.001 g/l, $MnCI_2$ 0.001 g/l, pH 7.0). This cell culture medium could be used directly as sample for measuring chitinase activity. Because it hardly conreducing sugar such as glucose (blank value=0), the detected reducing sugar can be considered as a chitinase reaction product. The results can be used for easy preparation method for determination of enzyme activity and analysis of enzyme-substrate reaction in step of screening of chitinase producing bacteria.

• Korean Journal of Microbiology
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• v.13 no.1
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• pp.37-44
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• 1975

As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the gorwth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, $KH_2PO_4$ 1.0g, sucrose 30.0g, $MgSo_4$ $7H_2$O 0.5g, $FeCl_36H_2$O 1.0mg thiamine 200.gamma.g, and distilled water 1000ml, pH6.5.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.1
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• pp.51-56
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• 1998

A bacteium, KP-6408, capable of hydrolyzing fibrin was isolated from Chungkook-jang, which was possibly identified as a strain of Bacillus sp. The effects of culture condition and medium composition on the enzyme production were investigated. Among nitrogen sources tested, yeast extract was the most effective for the enzyme production, and the level of the concentration for the optimal enzyme production was 0.2%(w/v). For carbon sources, glucose was the best for the enzyme production with the level of 2.0%(w/v). The enzyme was maximally produced by cultivating the enzyme production with the level of 2.0%(w/v). The enzyme was maximally produced by cultivating the organism at the liquid medium of the initial pH 8.0 and temperature of 4$0^{\circ}C$. In Chungkook-jang fermentation, the enzyme was maximally produced when incubated at 35$^{\circ}C$ for 24 hrs using soybean as a solid medium. The addition of various rice starch to the soybean in Chungkook-jang fermentation lowered the enzyme production.

• Journal of the Korea Safety Management & Science
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• v.12 no.3
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• pp.189-195
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• 2010

ERP system has been spreaded from large companies to many small and medium sized companies, and it is recognized as a basic system for optimum and efficient work process. The focus is moving from manufacturing companies to various specialized companies. Once ERP system was introduced to those specialized companies, there was attempt to customize an ordinary system which used in manufacturing companies, but it was hard to take effect due to the lack of experience and an inadequate analysis of work process. Some of foreign ERP packages are possible to be constructed for some companies. However, they are originally aim at a large enterprise and it is also very difficult to construct and maintain. For this reason, small and midium sized companies are having a difficulty to construct ERP system. This study deals with the construction of ERP system for small and medium sized design engineering company and introduction of the crucial points throughout the construction and requirements for composition module and customization of each main module. Based on the findings of this study, the specialized non-manufacturing companies are expected to have efficient and practical application of ERP system when they select and customize the ERP package of manufacturing companies.

• Microbiology and Biotechnology Letters
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• v.29 no.2
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• pp.110-114
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• 2001

Isolation of BacilLus sp. producing multi-enzyme and optimization of medium conditions for its production using feedstuffs for probiotics were carried out in this study. A bacterium isolated from natural resources, namely Bacillus subtilis 4-3, has multi-enzyme activity (phytase. cellulase, xylanasc, protease, and amylase. In the culture of B. subtilis 4-3 using soybean meal and rice bran. relatively low phytate degradation was noted using whereas high phytate degradability was observed with wheat bran (80.63%). The optimal composition of medium using feedstuffs was 1.0% (w/v) soybean meal and 2% (w/v) molasses to yield high cell growth.