• Title/Summary/Keyword: medium component

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Enhanced Production of Recombinant Protein in Escherichia coli Using Silkworm Hemolymph

  • Kim Ji Eun;Kim Eun Jeong;Rhee Won Jong;Park Tai Hyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.4
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    • pp.353-356
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    • 2005
  • The effect of silkworm hemolymph on the expression of recombinant protein in Escherichia coli was investigated. The addition of silkworm hemolymph to the culture medium in­creased the production of recombinant $\beta$-galactosidase in E. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1, 3, and $5\%$ silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.

Construction of Scientific Impact Evaluation Model Based on Altmetrics

  • Li, Jiapei;Shin, Seong Yoon;Lee, Hyun Chang
    • Journal of information and communication convergence engineering
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    • v.15 no.3
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    • pp.165-169
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    • 2017
  • Altmetrics is an emergent research area whereby social media is applied as a source of metrics to evaluate scientific impact. Recently, the interest in altmetrics has been growing. Traditional scientific impact evaluation indictors are based on the number of publications, citation counts and peer reviews of a researcher. As research publications were increasingly placed online, usage metrics as well as webometrics appeared. This paper explores the potential benefits of altmetrics and the deep relationship between each metrics. Firstly, we found a weak-to-medium correlation among the 11 altmetrics and visualized such correlation. Secondly, we conducted principal component analysis and exploratory factor analysis on altmetrics of social media, divided the 11 altmetrics into four feature sets, confirming the dispersion and relative concentration of altmetrics groups and developed the altmetrics evaluation model. We can use this model to evaluate the scientific impact of articles on social media.

Three Phenolic Glycosides from Gastrodia elata

  • Chae, Sung-Wook;Lee, A-Yeong;Lee, Hye-Won;Yoon, Tae-Sook;Moon, Byeong-Cheol;Choo, Byung-Kil;Kim, Ho-Kyoung
    • Journal of Applied Biological Chemistry
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    • v.51 no.2
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    • pp.61-65
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    • 2008
  • Three phenolic glycosides, gastrodin (1), parishin (2), and parishin B (3) were isolated from the n-BuOH fraction of Gastrodia elata (Orchidaseae) by medium pressure liquid chromatography. Their chemical structures were identified by the interpretation of their spectral data and direct comparison with literature values. Gastrodin and parishin were isolated as the major constituents in G. elata. Gastrodin may be a suitable index component for quality control of G. elata.

Properties of Urethane-Based IPN Elastomers (우레탄을 기초로 한 IPN 탄성체의 성질)

  • Min, Seong-Kee;Park, Chan-Young
    • Journal of the Korean Graphic Arts Communication Society
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    • v.22 no.2
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    • pp.37-54
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    • 2004
  • IPNs have interpenetrating motion and phase separation behavior of independent polymers, respectively, and also these are affected by the physical interaction of polymer components. First of all, 2- and 3-component IPNs based on PU are prepared by combination with two or three components of PU, PMMA, PS and epoxy resin. And then the entire physical properties concerning morphological and mechanical behaviors of these IPNs are measured by employing FT-IR, SEM, Rheovibron, and DSC, etc. Compared with pure component, Tg's of IPN are shifted to higher temperature in all IPN kinds, and these are considered in attribution to internal movement of glass transition temperature or partial phenomenon of interpenetration. Rheovibron measurement results in a broader distribution with peaks of tan${\delta}$ or E", and this morphologically represents a medium degree of partially mixed IPNs in confirmation of SEM photographs.

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Along-wind simplified analysis of wind turbines through a coupled blade-tower model

  • Spagnoli, Andrea;Montanari, Lorenzo
    • Wind and Structures
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    • v.17 no.6
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    • pp.589-608
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    • 2013
  • A model is proposed to analyse the along-wind dynamic response of upwind turbines with horizontal axis under service wind conditions. The model takes into account the dynamic coupling effect between rotor blades and supporting tower. The wind speed field is decomposed into a mean component, accounting for the well-known wind shear effect, and a fluctuating component, treated through a spectral approach. Accordingly, the so-called rotationally sampled spectra are introduced for the blades to account for the effect of their rotating motion. Wind forces acting on the rotor blades are calculated according to the blade element momentum model. The tower shadow effect is also included in the present model. Two examples of a large and medium size wind turbines are modelled, and their dynamic response is analysed and compared with the results of a conventional static analysis.

A Study on Optimized Layout Design of FAS Line Through Production Flow Analysis (생산 흐름 분석을 통한 FAS라인의 최적 배치 설계에 관한 연구)

  • Ga, Chun-Sik;Jung, Jin-Hwi;Jang, Bong-Choon;Eom, Yong-Gyun
    • Proceedings of the KSME Conference
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    • 2004.04a
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    • pp.1070-1075
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    • 2004
  • Many mid-sized companies in Korean automotive industry have attempted to solve the lack of human power, to control the quality of components, to improve the production rate, and to innovate the manufacturing line. The goals of this study are to analyze the production rate of an automotive component manufacturing line using simulation software, to construct a Flexible Automation Subassembly (FAS) system and to suggest an optimized layout design using FAS line. In this research, the simulation model for manufacturing line was developed and used the realistic data (production planning, component type, working order, process time, queue time, line rules, etc) of a medium sized company in Korean automotive industry. To complete this research, a simulation software 'ARENA' was used.

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Single Image Dehazing Using Linear Transformation of Saturation (채도의 선형 변환을 이용한 단일 영상 안개 제거)

  • Park, Taehee
    • IEMEK Journal of Embedded Systems and Applications
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    • v.14 no.4
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    • pp.197-205
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    • 2019
  • In this paper, an efficient single dehazing algorithm is proposed based on linear transformation by assuming that a linear relationship exists in saturation component between the haze image and haze-free image. First, we analyze the linearity of saturation channel, estimate the medium transmission map in terms of the saturation component. Then, the intensity of haze-free image is assumed by using CLAHE to enhance contrast of haze image. Experimental results demonstrate that proposed algorithm can naturally recover the image, especially can remove color distortion caused by conventional methods. Therefore, our approach is competitive with other state-of-the art single dehazing methods.

A Medium Access Control Scheme for Reducing Energy Consumption through Avoiding Receipt of Redundant Messages in Wireless Sensor Networks (무선 센서 네트워크에서 중복 메세지 순신 회피를 통한 에너지 소비절감 매체 접근 제어)

  • Han, Jung-An;Lee, Moon-Ho
    • Journal of Information Technology Applications and Management
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    • v.12 no.4
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    • pp.13-24
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    • 2005
  • The sensor network is a key component of the ubiquitous computing system which is expected to be widely utilized in logistics control, environment/disaster control, medical/health-care services, digital home and other applications. Nodes in the sensor network are small-sized and exposed to adverse environments. They are demanded to perform their missions with very limited power supply only. Also the sensor network is composed of much more nodes than the wireless ad hoc networks are. In case that some nodes consume up their power capacity, the network topology should change, and rerouting/retransmission is necessitated. Communication protocols studied for conventional wireless networks or ad hoc networks are not suited for the sensor network resultantly. Schemes should be devised to control the efficient usage of node power in the sensor network. This paper proposes a medium access protocol to enhance the efficiency of energy consumption in the sensor network node. Its performance is analyzed by simulation.

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Performance Analysis, Real Time Simulation and Control of Medium-Scale Commercial Aircraft Turbofan Engine

  • Kong, Chang-Duk;Jayoung Ki;Chung, Suk-Chou
    • Journal of Mechanical Science and Technology
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    • v.15 no.6
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    • pp.776-787
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    • 2001
  • The turbofan engine performance analysis for a medium scale commercial aircraft was carried out and the LQR control scheme for performance optimization was studied. By using scaled component maps from well-known CF6 engine characteristics, the steady-state performance analysis result was compared with BR715-56 engine performance data. The transient performance analysis was performed with four fuel schedules. The linear simulation was done at the maximum take-off condition. The real time linear simulation was performed by interpolation of the system matrices, which used the least square method as the function of LPC rotational speed. By using linear system matrices of design point, the LQR controller which used control variables for the fuel flow and the LPC bleed air was designed.

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Effect of Carbon Source and Carbon to Nitrogen Ratio on Carotenogenesis of Rhodotorula glutinis

  • Nam, Hee-Sop
    • Journal of Microbiology and Biotechnology
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    • v.1 no.1
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    • pp.75-78
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    • 1991
  • The carotenoid biosynthesis of a red oleaginous yeast, Rhodotorula glutinis was significantly changed when the yeast was grown on different carbon substrates. The highest carotenoid production was obtained on culture medium containing glucose when the carbon to nitrogen ratio (C/N ratio) was adjusted to 25.7. Galactose stimulated the biosynthetic rate of torularhodin, a xanthophyll component of the yeast. With decreasing C/N ratio of the medium, significant changes of $\gamma$-carotene and torularhodin were observed such that increase in the torularhodin concentration was nearly equal to the decrease in $\gamma$-carotene. It was speculated that the nature of carbon substrate affected the metabolic rate of the cell, and accompanied by the different pattern of carotenoid accumulation in the cell.

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