• Mycobiology
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• v.35 no.2
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• pp.82-86
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• 2007

Ergone, a fungal metabolite derived from ergosterol, was previously isolated and identified from Polyporus umbellatus. Ergone is a major component of P. umbellatus known to have anti-aldosteronic diuretic effect and also displays cytotoxic activities. Most of mushroom's fruit bodies used for test contained less than 10 ${\mu}g/g$ of ergone. But P. umbellatus have larger amount of ergone than any other mushrooms. In order to improve the ergone production from the submerged culture of P. umbellatus, several factors including medium composition, culture conditions (temperature and pH) and different combinations of co-cultivation with various mycelia were studied. Among various carbon sources examined, starch proved to be most effective for the production of mycelia. The optimum pH and temperature for a flask culture of P. umbellatus mycelia were found to be 4.5 and $25^{\circ}C$, respectively. Under the optimized culture conditions, both the ergone production (86.9 ${\mu}g/g$) and mycelial growth (3.5 g/l) increased when P. umbellatus was cultured with Armillariella mellea. When the optimized conditions were applied, both mycelium and ergone production were significantly enhanced.

• Plant Resources
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• v.3 no.3
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• pp.226-232
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• 2000

The availability of enokitake cultural waste for Lentinus edodes cultivation was investigated, although hardwood sawdust has traditionally been used as a substrate for this fungus. Firstly, physiochemical characteristics of cultural waste were analysed. Secondly, mycelial growth characteristics and fruiting yields of L. edodes on waste treated in some methods were determined. Physiochemical characteristics of enokitake cultural waste showed that the millwaste complex was a little degraded by enokitake fungus and suggested the probability that most component lost by enokitake could be rice bran. Mycelia of L. edodes grew and fruited well on waste supplemented by fresh rice bran and Quercus sawdust although didn't on waste only. Mycelial growths of these fungi on waste were accelerated when supplemented by rice bran to the percent of 40(w/w) but decreased or suppressed at above ratios(30, 40%, w/w). Supplementations of oak sawdust at above 40%(w/w) of the waste and rice bran at 20%(w/w) of the sawdust allowed such a good mycelial growth as to be selected as a pertinent mixing ratio for fruiting medium. A fruiting yield on enokitake cultural waste supplemented by oak sawdust (at 40% of the waste, w/w) and rice bran (at 20% of the sawdust, w/w) was not inferior to that on oak sawdust supplemented by rice bran only (at 20% of the sawdust, w/w). These results indicated strongly the potentiality of enokitake cultural waste as raw materials for shiitake cultivating substrates.

• Journal of the Korean Society of Food Culture
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• v.28 no.6
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• pp.640-650
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• 2013

Soy sauce, a basic ingredient in Asian cuisine, is made of fermented soybeans, salt, water, and barley or wheat flour. The sensory characteristics of soy sauce are not only determined through its main ingredients but also by various flavor compounds produced during the fermentation process. This study was conducted to identify the sensory attributes of five different commercial soy sauce samples that differ in Meju types (traditional Meju or modified Meju) and usage types. Thirty three sensory attributes, including appearance (1), odor (16), flavor (14), and mouthfeel (2) attributes, were generated and evaluated by eight trained panelists. The collected data were analyzed by analysis of variance and principal component analysis. There were significant differences in the intensities of all sensory attributes among the soy sauce samples. Soy sauce made with traditional Meju had high intensity of fermented fish, beany, musty odors and salt, Cheonggukjang, fermented fish flavors. Whereas two soy sauces made with modified Meju were strong in alcohol, sweet, Doenjang, roasted soybean flavors. Two soy sauces for soup made with modified Meju had medium levels of briny, sulfury, fermented odors and bitter, chemical flavors and biting mouthfeel characteristic.

• Reproductive and Developmental Biology
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• v.33 no.3
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• pp.125-131
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• 2009

The aim of this study is to investigate the effect of porcine epididymal fluid (pEF) on in vitro-maturation and subsequent fertilization of porcine follicular oocytes. Porcine cumulus-oocytes complexes retrieved from antral follicles were cultured in tissue culture medium (TCM)-l99 supplemented with pEF of different concentrations. At 48 h after culture, development of oocytes to germinal vesicle (GV) breakdown, metaphase I, anaphase-telophase I, and metaphase II were examined Significant (p<0.05) increase in the proportion of oocytes developed to MII stage was observed in oocytes cultured in pEF-containing TCM-l99 than in oocytes cultured in pEF-free TCM-199 (46.2% vs 16.7%), which was a dose-dependent manner. Subsequently, the proportion of monospermic fertilization were significantly (p<0.05) increased in oocytes cultured in the TCM supplemented with pEF than those cultured in pEF-free TCM-199 (51.0% vs 24.1%). In the second series of experiment, the percentage of MII oocytes was significantly (p<0.05) increased after exposure of oocytes to pEF during the first 22 h period of culture than after exposure of oocytes to pEF during the next 24 h of culture, while no significant difference in the percentage of monospermy was observed. The results of this study demonstrate that pEF contains at least enhancing component(s) for nuclear maturation.

• Korean Journal of Microbiology
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• v.29 no.4
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• pp.250-257
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• 1991

An obligate methanol-oxidizing bacterium, Methylobacillus sp. strain SK1, which grows only on methanol was isolated from soil. The isolate was nonmotile Gram-negtive rod. It does not have internal membrane system. The colonies were small, whitish-yellow, and smooth. The guanine plus cytosine content of the DNA was 48 mol%. Cellular fatty acids consisted predominantly of large amounts of straight-chain saturated $C_{16:0}$ acid and unsaturated $C_{16:1}$ acid. The major ubiquinone was Q-8, and Q-10 was present as minor component. The cell was obligately aerobic and exhibited catalase, but no oxidase, activity. Poly-.betha.-hydroxybutyrate, endospores, or cysts were not observed. the isolate could grow only on methanol in mineral medium. Growth factors were not required. The isolate was unable to use methane, formaldehyde, formate, methylamine, and several other organic compounds tested as a sole source of carbon and energy. Growth was optimal at 35.deg.C and pH 7.5. It could not grow at 42.deg.C. The doubling time was 1.2h at 30.deg.C when grown with 1.0%(v/v) methanol. The growth was not affected by antibiotics inhibiting cell wall synthesis and carbon monoxide but was completely suppressed by those inhibiting protein synthesis. Methanol was found to be assimilated through the ribulose monophosphate pathway. Cytochromes of b-, c-, and o- types were found. Cell-free extracts contained a phenazine methosulfate-linked methanol dehydrogenase activity, which required ammonium ions as an activator. Cells harvested after the late exponential phase seemed to contain blue protein.ein.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.05a
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• pp.120-123
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• 2009

In our environment that is developed centered on the water such as emphasizing the importance of environment and discussing the river recovery, the role of medium 'water' has been stressed and the water-friendly space's development has given a sense of stability and refreshment to people's mind. Hence, the importance of river in the city is recognized. Accordingly, this paper is intended to describe the necessity of color management and to suggest the definite landscape formation plan based on it by collecting the type analysis and color landscape by different landscape type and by evaluating the color landscape image. It is expected that this paper will become utilized as the basic frame of case zone.

• Elastomers and Composites
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• v.35 no.3
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• pp.227-235
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• 2000

This study focused on the recycling technology and application of waste polyurethane scrap($5{\sim}7mm$) and waste urethane form from the footware scrap. Firstly we suggest the waste polyurethane can be used as a component of medium for hydroponic rose culture. Secondly, recycled thermoplastic polyurethane(RTPU) was produced and blended it with high impact polystyrene(HIPS). And also, in order to extend application of recycling field, the former was produced with adding the amine foaming agent to RTPU/HIPS alloy. The main purpose of this study is to diverse of the recycling of the waste polyurethane.

• Environmental Engineering Research
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• v.23 no.4
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• pp.406-419
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• 2018

In planning public service systems such as waterworks, the design population is very important factor. Owing to the limitations of the indirect method, two new models, which take into consideration urban characteristics, were developed to accurately predict external migration rate (EMR), which is an essential component in estimating reliably the design population. The root mean square error (RMSE) between the model values and observed values were 10.12 and 15.58 for the metropolitan cities and counties respectively and were lower compared to RMSE values of 27.31 and 28.79 obtained by the indirect method. Thus, the developed models provide a more accurate estimate of EMR than the indirect method. In addition, the major influencing factors for external migration in counties were development type, ageing index, number of businesses. On the other hand, the major influencing migration factors for cities were project scale, distance to city center, manufacturing size, population growth rate and residential environment. Future medium and long-term studies would be done to identify emerging trends to appropriately inform policy making.

• Journal of the Korean Society of Visualization
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• v.17 no.1
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• pp.78-84
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• 2019

A gas turbine is the main equipment of a power plant that generates electricity by high-speed rotation of the rotor in a high-temperature environment. In particular, in the case of medium to large-sized gas turbines, the rotor is composed of a plurality of stages, and each component is exposed to different physical environments. Especially, in the case of the tip clearance of the turbine, it is a very important factor in the performance of the design items and the operation of the stable turbine, and a design considering the physical behavior of all major parts should be done. In this study, we will discuss the process of visualizing the physical behavior of turbine operating conditions and the method of designing tip clearance for stable operation by using commercial finite element analysis program for gas turbine assembly model and single product.

• Journal of Korea Society of Digital Industry and Information Management
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• v.14 no.4
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• pp.1-7
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• 2018

The floating sculptures in the form of ad-ballon commonly used ropes in order to hold on. Relatively air flow is much less indoor than outdoor. Users of buoyancy sculptures hope to be able to maintain their desired posture without being fixed. This study applied drone technology to buoyancy sculptures. The drones can be moved vertically and horizontally, and the posture can be maintained, so buoyancy sculptures are easy to apply. Therefore, we have studied the control system of buoyancy sculpture using drone technology. Also, a control system that can maintain the desired posture at a constant height was studied. The overall shape was a light fiber material and helium gas for zero buoyancy to support the sculpture. The system configuration was STM32F103CB from ARM. In addition, the gyro and acceleration, geomagnetic sensors and motors are composed of small and medium size BLDC motors. The scheduling of the control system in the configuration of the control device was carefully considered. Because the role of the whole component becomes very important. The communication between the components is divided into the sensor fusion and the interface communication with the whole controller. Each communication technology is designed to expand. This study was implemented to actively respond from the viewpoint of posture control using the drone technology.