• 한국약용작물학회지
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• 제28권3호
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• pp.209-220
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• 2020

Background: This study investigated the effects of soil properties on the soil fungal community in first and continuous cultivation areas of Cnidium officinale Makino. Methods and Results: The soil fungal community was analyzed for relative abundance and principal coordinate analysis (PCoA) was conducted using Illumina MiSeq sequencing. The correlation between the soil chemical properties and the soil fungal community was assessed with distance-based linear models (DISTLM). The soil fungal community showed distinct clusters consisting in the continuous cultivation area of C. officinale Makino. PCoA and DISTLM indicated that soil pH, calcium, and available P₂O₅ significantly affected the soil fungal community in the cultivation area of C. officinale Makino. In addition, considering 5 different pathogenic fungi the relative abundance of Fusarium in the continuous cultivation area was significantly higher compared to that in the first cultivation area of C. officinale Makino. Conclusions: This study is important because it has determinined the effects of soil properties on the soil fungal community in both first and continuous cultivation areas of C. officinale Makino. Moreover, these results will be helpful to investigate the cause of continuous cropping obstacle in C. officinale Makino by examining the changes of soil fungal community.

• Journal of Ginseng Research
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• 제43권1호
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• pp.77-85
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• 2019

Background: Glomus intraradices is a species of arbuscular mycorrhizal fungi that, as an obligate endomycorrhiza, can form mutually beneficial associations with plants. Panax ginseng is a popular traditional Chinese medicine; however, problems associated with ginseng planting, such as pesticide residues, reduce the ginseng quality. Methods: In this experiment, we studied the effect of inoculating G. intraradices on several physiological properties and microbial communities of ginseng. UV-Visible Spectrum method was used to detect physical properties. Denaturing gradient gel electrophoresis method was used to analyze microbial communities. Results: The results indicated that inoculation with G. intraradices can improve the colonization rate of lateral ginseng roots, increase the levels of monomeric and total ginsenosides, and improve root activity as well as polyphenol oxidase and catalase activities. We also studied the bacterial and fungal communities in ginseng rhizospheric soil. In our study, G. intraradices inoculation improved the abundance and Shannon diversity of bacteria, whereas fungi showed a reciprocal effect. Furthermore, we found that G. intraradices inoculation might increase some beneficial bacterial species and decreased pathogenic fungi in rhizospheric soil of ginseng. Conclusion: Our results showed that G. intraradices can benefit ginseng planting which may have some instructive and practical significance for planting ginseng in farmland.

• Journal of Microbiology and Biotechnology
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• 제34권7호
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• pp.1452-1463
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• 2024

Fungi generate different metabolites some of which are intrinsically bioactive and could therefore serve as templates for drug development. In the current study, six endophytic fungi namely Aspergillus flavus, Aspergillus tubigenesis, Aspergillus oryzae, Penicillium oxalicum, Aspergillus niger, and Aspergillus brasiliensis were isolated and identified from the medicinal plant, Silybum marianum. These endophytic fungi were identified through intra transcribed sequence (ITS) gene sequencing. The bioactive potentials of fungal extracts were investigated using several bioassays such as antibacterial activity by well-diffusion, MIC, MBC, anti-biofilm, antioxidant, and haemolysis. The Pseudomonas aeruginosa PAO1 was used to determine the antibiofilm activity. The ethyl acetate extract of Aspergillus flavus showed strong to moderate efficacy against Staphylococcus aureus, Escherichia coli, P. aeruginosa, and Bacillus spizizenii. Aspergillus flavus and Aspergillus brasiliensis exhibited significant antibiofilm activity with IC₅₀ at 4.02 and 3.63 mg/ml, while A. flavus exhibited maximum antioxidant activity of 50.8%. Based on HPLC, LC-MS, and NMR experiments kojic acid (1) and carbamic acid (methylene-4, 1-phenylene) bis-dimethyl ester (2) were identified from A. flavus. Kojic acid exhibited DPPH free radical scavenging activity with an IC₅₀ value of 99.3 ㎍/ml and moderate activity against ovarian teratocarcinoma (CH1), colon carcinoma (SW480), and non-small cell lung cancer (A549) cell lines. These findings suggest that endophytic fungi are able to produce promising bioactive compounds which deserve further investigation.

• 한국균학회지
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• 제48권3호
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• pp.251-271
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• 2020

Diversity and community composition of bulb-associated fungi of Fritillaria Cirrhosae Bulbus source plants, which are used in traditional chinese medicine, in the eastern Himalaya-Hengduan Mountains, southwestern China, were estimated based on the internal transcribed spacer rDNA sequence analysis, using host plant species, geographic area, and plant phenology as variables. A total of 1,486 fungal sequences assigned to 251 operational taxonomical units (OTUs) were obtained from the bulbs. Fungal OTUs comprised 96.41% Ascomycotina, 3.52% Basidiomycotina, and 0.07% Zygomycotina. Sordariomycetes, Hypocreales, and Nectriaceae were the most frequent fungal lineages at each taxonomic rank. Fusarium, Ilyonectria, Tetracladium, Leptodontidium, and Tomentella were the top OTU-rich genera. Fusarium sp. 03, Ilyonectria rufa, Fusarium sp. 08, Ilyonectria sp. 03, and Leptodontidium orchidicola 03 represented the most frequent OTUs. Fusarium spp. were the most frequent general taxa. The distribution of fungal community exhibited preferences for host plant species, geographic area, and plant phenology. These findings are the foundation of our research on culturing and active metabolites of bulb-associated fungi of Fritillaria Cirrhosae Bulbus source plants.

• Archives of Pharmacal Research
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• 제23권1호
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• pp.46-49
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• 2000

Two neolignan compound, magnolol $(5,5^{l}-diallyl-2,2^{l}-dihydroxybiphenyl, 1)$ and honokiol $(5,5^{l}-diallyl-2,4^{l}-dihydroxybiphenyl, 2)$ were isolated from the stem bark of Magnolia obovata and evaluated for antifungal activity against various human pathogenic fungi. Compound 1 and 2 showed significant inhibitory activities against Trichophyton mentagrophytes, Microsporium gypseum, Epidermophyton floccosum, Aspergillus niger, Cryptococcus neoformans, and Candida albicans with minimum inhibitory concentrations (MIC) in a range of $25-100{\mu}g/ml$. Therefore, compound 1 and 2 could be used as lead compounds for the development of novel antifungal agents.

• Mycobiology
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• 제34권3호
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• pp.120-123
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• 2006

Phytoterpenoid isolated from Artabotrytis odoratissimus inhibited spore germination of some plant pathogenic as well as saprophytic fungi e.g. Alternaria alternata, A. solani, Cercospora sp., Curvularia maculans, C. pennisetti, Fusarium udum, Helminthosporium echinochlova, H. frumentacie, H. penniseti and Ustilago cynodontis. In Curvularia maculans and H. frumentacie, spore germination was completely inhibited at 2000 ppm. However, Curvularia maculans and C. pennisetti showed considerable sensitivity to this chemical even at 500 ppm.

• 한국균학회지
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• 제7권2호
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• pp.87-90
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• 1979

Attempts were made to investigate sterol components of Schizophyllum commune Fr. which is one of medicinal fungi and grows wildly in Korea. Its carpophores were collected in Mountain Gwanak and extracted with chloroform and methanol. A sterol was isolated and identified as ergosterol by TLC, GLC and chemical tests.

• 한국균학회지
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• 제6권1호
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• pp.1-4
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• 1978

Attempts were made to investigate the sterol components of Coriolus versicolor (Fr.) Quel, which is one of medicinal fungi and which grows wildly in Korea.Its carpophores were collected in the Gyeong Gi Province and extracted with chloroform and methanol. Four Compounds were isolated and one of these compounds was identified as ergosterol by T.L.C., G.L.C. and chemical tests. ${\beta}-Sitosterol$ appears to exist also in the carpophore.

• Journal of Ginseng Research
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• 제44권3호
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• pp.506-518
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• 2020

Background: Red-skin root of Asian ginseng (Panax ginseng) significantly reduces the quality and limits the production of ginseng in China. The disease has long been thought to be a noninfectious physiological disease, except one report that proved it was an infectious disease. However, the causal agents have not been successfully determined. In the present study, we were to reveal the pathogens that cause red-skin disease. Methods: Ginseng roots with red-skin root symptoms were collected from commercial fields in Northeast China. Fungi were isolated from the lesion and identified based on morphological characters along with multilocus sequence analyses on internal transcription spacer, β-tubulin (tub2), histone H3 (his3), and translation elongation factor 1α (tef-1α). Pathogens were confirmed by inoculating the isolates in ginseng roots. Results: A total of 230 isolates were obtained from 209 disease samples. These isolates were classified into 12 species, including Dactylonectria sp., D. hordeicola, Fusarium acuminatum, F. avenaceum, F. solani, F. torulosum, Ilyonectria mors-panacis, I. robusta, Rhexocercosporidium panacis, and three novel species I. changbaiensis, I. communis, and I. qitaiheensis. Among them, I. communis, I. robusta, and F. solani had the highest isolation frequencies, being 36.1%, 20.9%, and 23.9%, respectively. All these species isolated were pathogenic to ginseng roots and caused red-skin root disease under appropriate condition. Conclusion: Fungal complex is the causal agent of red-skin root in P. ginseng.

• 한국자원식물학회지
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• 제35권4호
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• pp.428-434
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• 2022

감초의 건조 방법 및 감마선 조사를 이용하여 미생물 저감화 효과를 확인하였다. 또한 감마선 조사 때문에 성분 변화가 있는지 확인하기 위해서 HPLC를 이용하여 유효성분인 Glycyrrhizin, Liquiritin의 성분 변화를 확인하였다. 건조 방법으로는 마이크로웨이브 건조, 동결건조, 열풍건조, 자연건조 순으로 미생물 저감화에 효과적이었고, 감마선 조사 후에는 모든 건조 시료에서 미생물이 사멸되었음을 확인하였으며, 감마선 조사에 따른 유효성분 함량의 수치 변화는 있었으나 통계적으로 유의성이 없게 나타났다.