• Title/Summary/Keyword: mechanical mixing method

검색결과 377건 처리시간 0.025초

마이크로 혼합기와 반응기로 구성된 DNA 결찰용 바이오칩에 관한 연구 (A Study About Biochip Combined with Micro Mixer and Reactor for DNA Ligation)

  • 강도형;안유민;황승용
    • 대한기계학회논문집A
    • /
    • 제32권8호
    • /
    • pp.624-632
    • /
    • 2008
  • In this research, we developed new PDMS-glass based microbiochip consisted of the micromixer and microreactor for DNA ligation. The micromixer was composed of a straight channel integrated with nozzles and pillars, and the microreactor was composed of a serpentine channel. We coated the PDMS chip surface with the 0.25wt.% PVP solution to prevent the bubble generation which was caused by the hydrophobicity of the PDMS. The new micomixer was passive type and the mixing was enhanced by a convective diffusion using the nozzle and pillar. The 10.33mm long micromixer showed the good mixing efficiency of 87.7% at 500 l/min flow rate. We could perform the DNA ligation successfully in the microbiochip, and the ligation time was shortened from 4 hours in conventional laboratory method to 5 min in the microbiochip.

공침법에 의한 소결첨가제의 균일혼합이 $Si_3N_4$ 상압소결체의 기계적 특성에 미치는 영향 (Effect of Homogeneous Mixing of Sintering Additives by Coprecipitation Method on Mechanical Properties of Pressureless-Sintered $Si_3N_4$)

  • 김지순
    • 한국세라믹학회지
    • /
    • 제30권4호
    • /
    • pp.265-272
    • /
    • 1993
  • Effect of mixing homogeneity in powder mixtures of Si3N4 and sintering additive (10mol% YAG) prepared by coprecipitation on the mechanical properties of pressureless-sintered body was investigated. Sintering was performed in a graphtie furnace at 1850℃ for 0.5h under 0.15MPa N2 atmosphere using Si3N4 powder bed. Results from the measurement of Young's modulus, Vickers hardness, 4-point-bending strength, and KIC for the coprecipitation-treated (CP) and the mechanically-mixed specimens(MM) showed that a remarkable improvement in flexural strength and Weibull parameter can be achieved for the CPspecimens: (677±68MPa, 12.0) for CP samples and (539±108MPa, 5.5) for MM. Other properties were almost same irrespective of preparation methods.

  • PDF

PIV/LIF기법에 의한 교반혼합기 내의 속도장과 농도장 동시 측정 (Simultaneous Measurement of Velocity and Concentration Field in a Stirred Mixer Using PIV/LIF Technique)

  • 정은호;윤상열;김경천
    • 대한기계학회논문집B
    • /
    • 제27권4호
    • /
    • pp.504-510
    • /
    • 2003
  • Simultaneous measurements of turbulent velocity and concentration field in a stirred mixer tank are carried out by using PIV/LIF technique. Instantaneous velocity fields are measured with a 1K$\times$1K CCD camera adopting the frame straddle method while the concentration fields are obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. It is found that the general features of the mixing pattern are quite dependent on the local flow characteristics during the rapid decay of mean concentration. However, the small scale mixing seems to be independent on the local turbulent velocity fluctuation.

재순환 영역이 있는 축대칭 난류 확산화염의 구조 (II) (The Structure of Axisymmeric Turbulent Diffusion Flame(II))

  • 이병무;신현동
    • 대한기계학회논문집
    • /
    • 제10권1호
    • /
    • pp.70-77
    • /
    • 1986
  • 본 논문에서는 재순환 영역이 존재하는 축대칭 난류 확산화염 구조예측의 전 단계로서, 이미 발표된 바있는 필자의 실험 데이터를 바탕으로 하여 등온유동에 서의 난류모델을 검토한다. 유선의 곡률이 큰 유동에 2방정식 모델을 적용함은 큰 결점을 보완한 수정-2방정식모델을 채택하여 실험결과와 비교, 검토하여 모델의 타당성을 조사하였다.

분산 방법에 따른 탄소나노섬유/에폭시 복합재료의 기계적 물성에 관한 연구 (A Study on Mechanical Properties of Carbon Nanofiber/Epoxy Composites with Dispersion Methods)

  • 공진우;정상수;김태욱
    • 한국복합재료학회:학술대회논문집
    • /
    • 한국복합재료학회 2004년도 춘계학술발표대회 논문집
    • /
    • pp.151-154
    • /
    • 2004
  • Despite of the excellent properties of carbon nanofiber, The properties of carbon nanofiber filled polymer composites were not increased largely. The reason is that it is still difficult to ensure the uniform dispersion of carbon nanofiber in a polymer matrix. In this study, For improvement properties of carbon nanofiber filled epoxy composites, the effect of dispersion was investigated. The compounds were prepared by two methods, solution blending and mechanical mixing. Mixing of solution blending method was used using ultrasonic. Dispersion of carbon nanofiber was observed by optical microscope and scanning electron microscope (SEM). UV adsorption and turbidity measured by UV spectrometer was used for the comparison of dispersion of carbon nanofiber.

  • PDF

이유체 분무기의 분무특성 및 노즐설계 실험 (Spray characteristics and nozzle design experiment to twin-fluid atomizer)

  • 정진도;지평삼
    • 대한기계학회논문집B
    • /
    • 제20권6호
    • /
    • pp.1941-1947
    • /
    • 1996
  • Droplet size measurement technique was established for the sprayed viscous fluid by virtue of the installation of the sprayed-droplet size measurement system employing light scattering method. Atomization test results showed that the mean droplet size of the sprayed viscous fluid is decreased with the increase of the mass ratio of air to fuel and in case of the same air/fuel ratio, also with the increase of viscous fluid flow rate, and is increased with the distance from atomizer tip. Basic design data for the manufacture of external-mixing type, Y-Jet type, and internal-mixing type atomizers was acquired from the atomization tests.

MA 316L ODS 및 Wet 316L ODS 스테인리스강에서 충격에너지에 미치는 소결 공정의 영향 (Effects of the Sintering Variable on Impact Energy in MA 316L ODS and Wet 316L ODS Stainless Steels)

  • 김성수;한창희;장진성
    • 한국분말재료학회지
    • /
    • 제17권2호
    • /
    • pp.113-122
    • /
    • 2010
  • Two kinds of oxide-dispersion-strengthened (ODS) 316L stainless steel were manufactured using a wet mixing process(wet) and a mechanical alloying method (MA). An MA 316L ODS was prepared by a mixing of metal powder and a mechanical alloying process. A wet 316L ODS was manufactured by a wet mixing with 316L stainless steel powder. A solution of yttrium nitrate was dried after being in the wet 316L ODS alloy. The results showed that carbon and oxygen were effectively reduced during the degassing process before the hydroisostatic process (HIP) in both alloys. It appeared that the effect of HIP treatment on increase in impact energy was pronounced in the MA 316L ODS alloy. The MA 316L ODS alloy showed a higher yield strength and a smaller elongation, when compared to the wet 316L ODS alloy. This seemed to be attributed to the enhancement of bonding between oxide and matrix particles from HIP and to the presence of a finer oxide of about 20 nm from the MA process in the MA 316L ODS alloy.

이산 요소법을 이용한 골재 입자의 혼합 및 배출 시 골재 거동 및 강판 마모에 관한 연구 (Discrete Element Method for Defining the Dynamic Behavior and Abrasion of Gravel in Mixer Trucks during Mixing and Discharging)

  • 유승훈;우호길
    • 한국기계가공학회지
    • /
    • 제19권12호
    • /
    • pp.34-41
    • /
    • 2020
  • Ready-mixed concrete is unconsolidated concrete typically transported to construction sites by using mixer trucks. A proper rotation of concrete is necessary to prevent its solidification in mixer trucks during transport: in accordance with the manufacturing method and quality inspection prescribed in KSF4009, this movement is maintained after the manufacturing of concrete in professional production plants and the addition of water, solid materials, and admixtures. Unfortunately, mixer truck parts wear out over long periods of time. In order to improve the wear resistance of the main part of mixer trucks, we used a steel plate with good wear resistance or partially added a reinforcement plate. In this study, we first tested the properties of concrete (as required for the DEM), and then carried out mixing and discharge simulations to define the actual operating conditions of mixer trucks. For each condition, we calculated the amount and location of wear. The reliability of our results was finally verified by comparing them with the measurement values. Overall, this study provided basic data for an optimal design of mixer trucks: one that would reduce the vehicles' weight and production costs.

2단계 배합방법으로 제조된 순환굵은골재 콘크리트의 장기재령 역학적 성능 및 내구성 평가 (Evaluation on Long-term Mechanical Performance and Durability of Recycled Coarse Aggregate Concrete Produced by Two-stage Mixing Approach)

  • 허성욱;손정진;정철우;김영찬
    • 한국건설순환자원학회논문집
    • /
    • 제10권4호
    • /
    • pp.475-481
    • /
    • 2022
  • 순환골재는 생산과정에서 발생하는 미세균열 및 순환골재 표면에 존재하는 시멘트 페이스트 성분의 존재로 인해, 천연골재에 비해 높은 흡수율을 가지며, 이로 인해 콘크리트에 골재로서 사용할 경우 작업성 저하, 강도의 저하 등의 문제가 발생하게 된다. 골재의 수급이 점점 어려워지고 있는 현 상황에, 골재 수급의 대안이 될 수 있는 순환골재를 적극적으로 활용하기 위한 방법으로, 본 연구에서는 Tam et al.이 제안한 2단계 배합과정을 활용하였고, 이를 이용해 제조한 콘크리트의 압축강도, 탄성계수, 및 염소이온 확산계수를 일반 배합과정을 적용한 천연골재 콘크리트 및 100 % 치환율의 순환골재 콘크리트와 비교 분석하였다. 실험 결과에 따르면, 압축강도와 탄성계수는 물시멘트비에 관계없이, 2단계 배합방법으로 제작한 순환골재 콘크리트가 일반배합과정으로 배합된 순환골재 콘크리트에 비해 높게 나타났으며, 천연골재 콘크리트에 근접하는 역학적 성능을 발휘하였다. 그러나 염소이온 확산계수의 경우 순환골재 콘크리트가 천연골재 콘크리트보다 높게 측정되었으며, 2단계 배합과정의 적용에 따른 염소이온 확산계수의 감소는 확인할 수 없어, 2단계 배합이 내구성에는 크게 기여하지 않는 것으로 판단된다.