• 제목/요약/키워드: mec A

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Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples

  • Can, Hayriye Yesim;Elmali, Mehmet;Karagoz, Alper
    • 한국축산식품학회지
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    • 제37권2호
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    • pp.175-180
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    • 2017
  • The objectives of this study were: i) to detect the presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk, cheese, beef minced meat, and chicken meat samples; ii) to evaluate the antimicrobial susceptibility of the isolates; and iii) to determine clonal relation among the isolates by using pulsed-field gel electrophoresis (PFGE) method. Therefore, a total of 160 food samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey. Twenty (12.5%) of the samples were found to be contaminated with S. aureus. A total of 40 isolates from the 20 positive samples were confirmed to be S. aureus by multiplex PCR based on 16S rRNA and nuc gene. The mec A gene was not detected in any of the S. aureus strains. In the present study, 39 out of 40 (97.5%) isolates were found to be resistant to one or more antibiotics. All of isolates were susceptible to gentamicin, oxacillin, and vancomycin. The highest resistance rate was detected in penicillin (95%) and ampicillin (92.5%), followed by tetracycline (30%), erythromycin (20%), ciprofloxacin (12.5%). Nine major patterns were determined by PFGE. In 6 of these patterns, thirty-six strains (90%) had identical PFGE profiles.

Effects of arsenite and variation of microbial community on continuous bio-hydrogen production from molasses using a sequence batch reactor (SBR)

  • William, Dennis Sambai;Lee, Pul-eip;Lee, Tae-jin
    • Environmental Engineering Research
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    • 제20권4호
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    • pp.370-376
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    • 2015
  • This study investigated the effects of various arsenite concentrations on bio-hydrogen production from molasses using a sequence batch reactor (SBR) operated in a series of three batch cycles. In the first batch cycle, hydrogen production was stimulated at arsenite concentrations lower than 2.0 mg/L, while inhibition occurred at arsenite concentration higher than 2.0 mg/L compared to the control. Hydrogen production decreased substantially during the second batch cycle, while no hydrogen was produced during the third batch cycle at all tested concentrations. The toxic density increased with respect to the increase in arsenite concentrations (6.0 > 1.6 > 1.0 > 0.5 mg/L) and operation cycles (third cycle > second cycle > first cycle). The presence of microorganisms such as Clostridium sp. MSTE9, Uncultured Dysgonomonas sp. clone MEC-4, Pseudomonas parafulva FS04, and Uncultured bacterium clone 584CL3e9 resulted in active stimulation of hydrogen production, however, it was unlikely that Enterobacter sp. sed221 was not related to hydrogen production. The tolerance of arsenite in hydrogen producing microorganisms decreased with the increase in induction time, which resulted in severing the inhibition of continuous hydrogen production.

Ambient Air Waste Sorting Facilities Could Be a Source of Antibiotic Resistant Bacteria

  • Calheiros, Ana;Santos, Joana;Ramos, Carla;Vasconcelos, Marta;Fernandes, Paulo
    • 한국미생물·생명공학회지
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    • 제49권3호
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    • pp.367-373
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    • 2021
  • The antimicrobial resistance of Staphylococcus spp. and Gram negative strains present in air samples from waste sorting facilities was assessed. Phenotypic studies have revealed a high percentage of strains of Staphylococcus spp. resistant to methicillin. Genotypically and by RT-PCR, it was found that the mecA gene usually associated with methicillin resistance was present in 8% of the Staphylococcus strains isolated. About 30% of the Gram negative strains from the same samples also displayed resistance to meropenem and 79% of these were resistant to multiple antibiotics from different classes, namely cephalosporins and β-lactams. The results suggest that in professional activities with high levels of exposure to biological agents, the quantification and identification of the microbial flora in the work environment, with the determination of the presence of potential agents displaying multi-resistances is of relevance to the risk assessment. The personal protection of workers is particularly important relevance in these cases, since many of the strains that exhibit multi-resistance are potential opportunistic agents.

A Survey of Computational Offloading in Cloud/Edge-based Architectures: Strategies, Optimization Models and Challenges

  • Alqarni, Manal M.;Cherif, Asma;Alkayal, Entisar
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제15권3호
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    • pp.952-973
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    • 2021
  • In recent years, mobile devices have become an essential part of daily life. More and more applications are being supported by mobile devices thanks to edge computing, which represents an emergent architecture that provides computing, storage, and networking capabilities for mobile devices. In edge computing, heavy tasks are offloaded to edge nodes to alleviate the computations on the mobile side. However, offloading computational tasks may incur extra energy consumption and delays due to network congestion and server queues. Therefore, it is necessary to optimize offloading decisions to minimize time, energy, and payment costs. In this article, different offloading models are examined to identify the offloading parameters that need to be optimized. The paper investigates and compares several optimization techniques used to optimize offloading decisions, specifically Swarm Intelligence (SI) models, since they are best suited to the distributed aspect of edge computing. Furthermore, based on the literature review, this study concludes that a Cuckoo Search Algorithm (CSA) in an edge-based architecture is a good solution for balancing energy consumption, time, and cost.

Multidrug resistance of coagulase-negative staphylococci isolated from rescued wild animals

  • Rhim, Haerin;Kim, Hong-Cheul;Na, Ki-Jeong;Han, Jae-Ik
    • 한국동물위생학회지
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    • 제42권4호
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    • pp.251-255
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    • 2019
  • Wildlife is a bio-indicator of environmental pollution by antimicrobial resistant bacteria or genes, however, there is no information on antimicrobial resistance in wildlife-origin bacteria. This study aimed to investigate the normal microbiota of staphylococci and their antimicrobial resistance in wildlife that did not take any antimicrobials. After sampling and bacterial isolation/identification, antimicrobial resistance profiles were examined by broth microdilution test, Kirby-Bauer disc diffusion test and mecA genetargeted PCR. Of 90 isolates from wildlife, 83 were coagulase-negative staphylococci while only 7 were coagulase-positive staphylococci. Methicillin-resistance was found in 63 (70%) isolates and 35 of 90 (38.9%) isolates were multidrug-resistant staphylococci. When considering that all of the animals did not take any medication or contacted any medical device before the sampling, the results indicate significantly high prevalence of antimicrobial resistance in wild environments. Further study would be necessary to investigate the transmission route of antimicrobial resistance.

tDCS와 요추 움직임 조절 운동이 만성요통환자의 정적균형과 요통장애지수에 미치는 영향 (Effect of tDCS and Lumbar Motor Control Exercise on Static Balance and Disability in Chronic Low Back Pain)

  • 정용식;신의주
    • 대한정형도수물리치료학회지
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    • 제23권2호
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    • pp.1-8
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    • 2017
  • Background: The purpose of this study was to investigate the effects on static balance and disability in chronic low back pain with lumbar rotation extension subgroup of trascranial direct current stimulation (tDCS) and lumbar motor control exercise (MCE). Methods: In 40 male low back pain with lumbar rotation extension subgroup subjects were recruited for the study. Subjects were randomly allocated into two groups. Experimental groups received tDCS and MCE, Control groups received sham-tDCS and MEC. Before and after intervention, measured in surface area, whole path length and Roland-Morris low back pain questionnaire (RMQ). Results: Showed a significant static balance and disability from the experimental groups compared to the control group. Showed a no significant RMQ score from experimental groups compared to the control group. Conclusions: tDCS and lumbar MCE showed the increased static balance in chronic low back pain with lumbar rotation extension subgroup.

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The Impact of Traditional Market Properties and Relationship Quality on Customer Value : Approach from the viewpoint of the Means-end Chain Theory

  • Cho, Hee-Young;Han, Sang-Ho;Yang, Hoe-Chang
    • 유통과학연구
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    • 제12권1호
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    • pp.13-19
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    • 2014
  • Purpose - This study investigated relationship quality and/or loyalty, from the viewpoint that merchants and consumers could develop the traditional market. It reorganized variables to find the conditions of values that could stimulate consumers' motives to revive the traditional market. Research Design, data, and methodology - This study employed 202 copies of effective questionnaires, based on the data of Yang & Ju (2012), to conduct correlation, regression, and structured equation modeling (SEM). Results - The results emphasized product and store atmosphere as store selection attributes to consider in the minimum error correction (MEC) model; service factor was not significant. Further, consumers valued relationship quality in the test of mediated effects of the sub-factors of store selection attributes, including consumers' social and emotional value. The relationship quality significantly influenced consumers' value in traditional markets that needed to improve and develop using several variables. Conclusions - This study revealed connections between attributes, consequences, and values using the causal relation model, to generate an optimal model based on a practical and theoretical background and proposed ways to obtain consumer-related information easily.

Prevalence and Characterization of Methicillin-Resistant Staphylococcus aureus in Raw Meat in Korea

  • Lim, Suk-Kyung;Nam, Hyang-Mi;Park, Hyun-Jung;Lee, Hee-Soo;Choi, Min-Jung;Jung, Suk-Chan;Lee, Ji-Yeon;Kim, Young-Cho;Song, Si-Wook;Wee, Sung-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제20권4호
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    • pp.775-778
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    • 2010
  • A total of 2,858 meat samples collected during 2003-2008 in Korea were investigated, and methicillin-resistant Staphylococcus aureus (MRSA) isolates were isolated from 1.0% (9/890) of beef, 0.3% (4/1,055) of pork, and 0.3% (3/913) of chicken meat samples, respectively. MRSA isolates showed the two sequence types (STs), ST72 from beef and pork and ST692 from chicken meat. MRSA isolates from beef and pork were Panton-Valentine leukocidin-negative, staphylococcal cassette chromosome mec type IVa strain with ST72, which is the most prevalent type of communityacquired MRSA in Korea. An identical pulse-field gel electrophoresis pattern was detected among 10 of 16 MRSA isolates: 9 strains from beef (n=5) and pork (n=4) in 2008, and one strain from beef in 2005.

냉간단조용 친환경 수용성 윤활제의 개발 (Development of Environmentally Friendly Water-Soluble Lubricant for Cold Forging)

  • 이인수;강종훈;김영량;제진수;고대철;김병민
    • 한국소성가공학회:학술대회논문집
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    • 한국소성가공학회 2006년도 춘계학술대회 논문집
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    • pp.195-198
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    • 2006
  • Zinc phosphate coating is required in nearly all steel cold forging operations. However, the chemical byproducts of this lubricant system are difficult to dispose of and have a negative environmental impact. In order to replace zinc phosphate based lubricants partially or completely, candidate lubricant has been developed in this study. The performance evaluation of these lubricants was conducted using the double cup extrusion test and spike forging test. With the use of the commercial FEM code DEFORM, friction factor calibration curves, i.e. cup height ratio vs. punch stroke and spike height vs. punch stroke, were established for different friction factor values. By matching the cup height ratio and the punch stroke and spike height vs. punch stroke from experiment to that obtained from FE simulations, the friction factor of the lubricants was determined. Three water-soluble lubricants; namely, Mec Homat, Royalcoat, and the developed lubricant were found to perform comparable to or better than zinc phosphate.

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Applications of 5G and 6G in Smart Health Services

  • Al-Jawad, Fatimah;Alessa, Raghad;Alhammad, Sukainah;Ali, Batoola;Al-Qanbar, Majd;Rahman, Atta-ur
    • International Journal of Computer Science & Network Security
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    • 제22권3호
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    • pp.173-182
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    • 2022
  • Healthcare organizations are overwhelmingly embracing smart value-based care strategies, which focuses on providing superior treatment at a significantly lower cost and quality of service (QoS). For these purposes, fifth generation (5G) of mobile service provides an innumerable improvement that clearly outperforms previous generations e.g., 3G and 4G. However, as with most advancements, 5G is projected to introduce new challenges, prompting the community to think about what comes next. This research was conducted to examine the most recent smart 5G technology applications and the solutions they provide to the healthcare industry. Finally, the paper discusses how the upcoming 6G technology has the potential to transform the future of healthcare sector even beyond the current 5G systems.