• Journal of Microbiology and Biotechnology
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• 제16권2호
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• pp.299-302
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• 2006

A combinatorial library of artificial transcription factors (ATFs) was introduced into the bacterial cells that expressed the Akt1-GFP fusion protein. By measuring the level of fluorescence generated by the transformed E. coli cells, we were able to obtain clones in which ATFs increased the solubility of the Akt1. Our results show that ATF library is a useful tool for increasing the solubility of selected recombinant proteins in E. coli.

• 한국산업융합학회 논문집
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• 제5권4호
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• pp.373-378
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• 2002

This paper presents a method to predict tool deflection induced by the thermal distribution and the cutting force using FEM in milling operation. The thermal distribution of cutting tool was predicted using FEM after measuring the temperature of the end of tool and of the tool holder. The thermal deflection of cutting tool was predicted using FEM as well. The tool deflection induced by the cutting force was analyzed with the solid model of cutting tool. An end mill tool caused most of tool deflection comparing to tool holder. Most of thermal deflection came from Z-direction and most of tool deflection induced by the cutting force came from X and Y direction. Precision cutting will be accomplished when tool locations are generated considering the thermal deflection of cutting tool and the tool deflection induced by the cutting force in CAD/CAM.

• 한국정밀공학회지
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• 제16권9호
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• pp.62-67
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• 1999

In milling, cutting tool ins directly attached to spindle and this tells that spindle can provide very useful information on the cutting tool condition such as wear or breakage. Since spindle is rotating at a high speed, measuring spindle velocity using a noncontacting measurement system gives the best information which can be obtained. Due to the force applied to spindle through cutting tool, velocity of spindle changes. And any change in cutting tool condition affects cutting force and consequently spindle vibration. With the intent of continuously monitoring cutting tool condition in intermittent machining operations in a benign manner, a noncontacting velocity measurement system using a laser Doppler velocimeter was assembled to measure spindle torsional vibration. Spindle vibration was measured and analysis of it in the frequency domain yielded a measure which corresponded to amount of cutting tool wear in milling.

• 한국정밀공학회지
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• 제11권6호
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• pp.185-199
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• 1994

This study has been performed to present a new automatic tool wear measurement by digital image processing. The purpose of this paper is to develop an automatic tool wear measuring system based on the image processing which can be applied to the quasi-real time measurement of the characteristics of insert tip in turning. Tool wear monitoring is one of the key-problems, for the development of control systems of modern unmanned factory which are not completely solved now. In oredr words at present complete qualitative and quantitative information on tool wear morphology is required, at least on the following aspects : flank wear, its dimensions and distribution on the maximum and mean values on VB pqrqmeter in the various zones of the wearland. crater wear, its main dimensions and values of KT parameters. This research has been performed to this technique made possible by designing a proper lighting system to the worn tool with following features : The flank wear is measured by observing the active cutting part from a proper direction and by lighting the wearland by a diffuser optic system. The crater wear is visualized by lighting the tool by a He-Ne gas laser system developed in this study. By means of this system it is research to evaluate classical parameters of tool wear and to have complete information about tool wear morphology.

• 간호행정학회지
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• 제13권1호
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• pp.17-23
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• 2007

Purpose: The purpose of this study was to develop the tool that can be used for measuring and appraising the performance of Oncology Advanced Practice Nurse(APN). Method: On the basis of some articles about roles of oncology APN, the researcher made a temporary tool and verified both content validity and face validity. Also the reliability and construct validity were verified. Result: The reliability of this tool was measured by internal consistency. Cronbachs' ${\alpha}$ was .92. The method of verification of construct validity was used known group technique because of small numbers of Oncology APN. Conclusions: This performance appraisal tool of Oncology APN was verified by both the reliability and construct validity. In future, this tool will be able to be used for appraisal and verifying of nursing care of Oncology APN.

• 한국생산제조학회지
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• 제7권6호
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• pp.80-89
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• 1998

Back rake angle of tool is one of the fundamental effects to the cutting ability. In this paper, for several back rake angle of lathe tool (-5$^{\circ}$ , 0$^{\circ}$ , 5$^{\circ}$ , 10$^{\circ}$ , 15$^{\circ}$ ), we experimentally examine cutting forces via orthogonal cutting. Using measured cutting forces, a formula for specific cutting resistance is derived according to the variation of tool angle. Also, the measured cutting forces are analyzed in both time and frequency domain. Cutting parameters are obtained by measuring the thickness of chip, and the effect of the back rake angle of tool is manifested. This study maintains the predicted cutting model with improved accuracy.

• 기술사
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• 제21권4호
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• pp.6-11
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• 1988

The study on the progress of automatic machining system has been active centering at the CNC machine tools recently to lead the applied production technology like unmaned machining to the practical level, and to make these application more efficient, they require detective method for tool wear and breakage while machining. In this study chiefly on turning, first derive cutting force about the tool surface by the cutting resistant sensor and with which change into electric signal and compare with the standards to make the tool life detection system to let know the tool life and study its application; the cutting resistance detected by the cutting resistant sensor has increased to the level of standards, the measuring system which can decide whether we change tools on the basis of comperative circuit is shown to be very reliable model for cutting tool life detection system.

• 한국생산제조학회지
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• 제20권1호
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• pp.53-59
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• 2011

End milling is available for machining the various shape of products and has been widely applied in many manufacturing industries. The quality of products depends on a machine tool performance and machining conditions. Recognition characteristics of the cutting condition is becoming a critical requirement for improving the utilization and flexibility of present-day CNC machine tools. The measurement of tool wear would be performed by coordinate-measuring machine(CMM). However, the usage of CMM requires much time and cost. In order to overcome the difficulties, on-line measurement(OLM) system was applied for a tool wear measurement. This study shows a reliable technique for the reduction of machining error components by developing a system using a CCD camera and machine vision to be able to precisely measure the size of tool wear in flat end milling for CNC machining. The CCD camera and machine vision attached to a CNC machine can determine tool wear quickly and easily.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2000년도 추계학술대회논문집 - 한국공작기계학회
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• pp.274-278
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• 2000

Ball end milling process is widely used in the die and mould manufacturing because of suitableness for the machining of free form surface. But, as ball end mill is long and thin, it is easily deflected by cutting force. In this study, Cutting force, tool deflection and surface precision was measured according to the change of depth and cutting location. Cutting force was acquired with tool dynamometer and a couple of eddy-current sensor measured tool deflection in x-y direction each. After machining, surface precision was measured with roundness tester and coordination measuring machine for sculptured surface angle change and cutting depth.

• 한국정밀공학회지
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• 제18권2호
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• pp.126-131
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• 2001

One of the major obstacles in testing or evaluating precisely the thermal behavior of a machine tool is the difficulty in measuring the heat transfer coefficients on the surfaces by a conventional method. This paper presents a new approach based on the inverse method to identify the values of heat transfer coefficients by using temperature changes measured on the surfaces of a machine tool during a short period in its operating. In the present method, a machine tool structure is modeled by the finite element method and the characteristic curves of the temperature change at several points on machine tool surfaces are theoretically derived in the form that they contain the heat transfer coefficient as an unfixed heat source are approximated so that the theoretical characteristic curves of temperature change fit the measured ones as closely as possible.