• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1276-1280
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• 2017

The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.

• Korean Journal of Acupuncture
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• 제26권1호
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• pp.85-109
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• 2009

Objective & Methods : The present study was to investigate the effect of Moutan Cortex Radicis Herbal-acupuncture (MCR-HA) at GB34 (Yangleungchean) on hyperlipidemia and arteriosclerosis induced by high fat diet in rats. Results : 1. Moutan Cortex Radicis herbal-acupuncture infusion solution (MCR-HAS) increased DPPH radical scavenging activity and HMG-CoA reductase inhibition rate in rat liver cells. 2. MCR-HA at GB34 decreased serum total cholesterol level, and increased serum phospholipid/total cholesterol in rats with high fat diet. 3. MCR-HA at GB34 decreased atherogenic index in rats with high fat diet. 4. MCR-HA at GB34 increased hepatic catalase activity in rats with high fat diet. Conclusion : These results suggested that Moutan Cortex Radicis herbal-acupuncture at GB34 has therapeutic effects on hyperlipidemia and related complications in rats with high fat diet.

• 대한인간공학회지
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• 제34권1호
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• pp.29-43
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• 2015

Objective: The aim of this study is to develop management guidelines and a procedure for an anthropometric suitability assessment of the main control room (MCR) in nuclear power plants (NPPs). Background: The condition of the MCR should be suitable for the work crews in NPPs. The suitability of the MCR depends closely on the anthropometric dimensions and ergonomic factors of the users. In particular, the MCR workspace design in NPPs is important due to the close relationship with operating crews and their work failures. Many documents and criteria have recommended that anthropometry dimensions and their studies are one of the foremost processes of the MCR design in NPPs. If these factors are not properly considered, users can feel burdened about their work and the human errors that might occur. Method: The procedure for the anthropometric suitability assessment consists of 5 phases: 1) selection of the anthropometric suitability evaluation dimensions, 2) establishment of a measurement method according to the evaluation dimensions, 3) establishment of criteria for suitability evaluation dimensions, 4) establishment of rating scale and improvement methods according to the evaluation dimensions, and 5) assessment of the final grade for evaluation dimensions. The management guidelines for an anthropometric suitability assessment were completed using 10 factors: 1) director, 2) subject, 3) evaluation period, 4) measurement method and criteria, 5) selection of equipment, 6) measurement and evaluation, 7) suitability evaluation, 8) data sharing, 9) data storage, and 10) management according to the suitability grade. Results: We propose a set of 17 anthropometric dimensions for the size, cognition/perception action/behavior, and their relationships with human errors regarding the MCR design variables through a case study. The 17 selected dimensions are height, sitting height, eye height from floor, eye height above seat, arm length, functional reach, extended functional reach, radius reach, visual field, peripheral perception, hyperopia/myopia/astigmatism, color blindness, auditory acuity, finger dexterity, hand function, body angle, and manual muscle test. We proposed criteria on these 17 anthropometric dimensions for a suitability evaluation and suggested an improvement method according to the evaluation dimensions. Conclusion: The results of this study can improve the human performance of the crew in an MCR. These management guidelines and a procedure for an anthropometric suitability assessment will be able to prevent human errors due to inadequate anthropometric dimensions. Application: The proposed set of anthropometric dimensions can be integrated into a managerial index for the anthropometric suitability of the operating crews for more careful countermeasures to human errors in NPPs.

• 시스템엔지니어링학술지
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• 제7권2호
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• pp.1-5
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• 2011

The purpose of the Environment Design (ED) for Main Control Room (MCR) of Nuclear Power Plant (NPP) is to provide and create an optimal working space to be free from physical, physiological and mental stress as well as environmental discomfort, based on the previous environment design experiences and to recommend the best ED including the color, the lighting and the interior design. Environment Design consists of three main areas: Human factor engineering design, Interior design with color design, and Lighting design. These design areas have been interactively cooperated in a way that each design specialist would share the objectives and concepts of the Environment Design for MCR. The specialists for Human Factors Engineering design had a corporative role in such a way to provide the guidelines for MCR design suitability of Interior and Lighting design considering the Human System Interface (HSI) safety concerns. This paper describes fruitful efforts to create the best fit for MCR ED among several design proposals with the design recommendations, impacts, and contributions to NPP environment.

• 대한환경공학회지
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• 제22권10호
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• pp.1869-1879
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• 2000

혐기성 소화공정 중 생성되는 주요 중간대사산물인 프로피온산의 분해대사에 대한 연구중 에탄올과의 산화 환원 반응인 coupling 반응으로써 혐기성 소화공정에서 프로피온산 축적을 저감시킬 수 있는 연구를 수행하였다. 따라서, 본 연구는 혐기성 공정에서 프로피온산의 전환에 따른 동력학적 반응과 에탄올과의 상호 반응에 따른 특정기질 선호영향을 모델에 적용하여 살펴보았다. 본 연구는 4단계의 실험으로 수행되었다. 1, 2, 3단계는 각기 다른 기질에 순화된 미생물들을 이용하여 프로피온산 1 g COD/L와 에탄올의 농도를 각각 0, 100, 200, 400과 1,000 mg/L로 주입하여 프로피온산과 에탄올의 혐기성 분해과정을 비교 연구하였으며, 4단계에서는 Glu-MCR과 HPr-MCR의 순화미생물의 혼합비를 조절하여 프로피온산 1 g COD/L를 주입하였을 때의 혐기성 분해를 연구하였다. 본 연구에서는 수정된 경쟁적 모델을 이용하여 특정기질 선호현상을 규명하였고, 에탄올 농도의 증가에 따라 아세트산 형성반응의 $K_{s2}$값의 증가와 메탄화 과정에서의 아세트산 생성 및 분해과정에 해당되는 $K_3$값이 일부 증가하는 결과를 얻을 수 있었다. 또한 순화미생물들에 따라 프로피온산과 에탄올의 분해에 미치는 영향이 다른 결과를 얻을 수 있었다.

• 대한전자공학회논문지TC
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• 제39권4호
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• pp.188-200
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• 2002

WRR 스케줄링 알고리즘은 계산의 단순성과 저 비용 구현의 장점을 가지고 있어서 고속 패킷 스위칭 네트워크에서 널리 쓰이고 있지만 버스티한 트래픽에 대해서는 입력 트래픽을 일정 비율로 스케쥴링하기 때문에 효율성이 낮다는 문제점이 있다. 또한, WRR 알고리즘은 ABR 서비스 클래스의 공정성 기준인 MCR plus equal share와 Maximum of MCR or Max-Min share 공평성을 만족시키지 못한다. 또한 Nabeshima et al의 기법은 여분 대역에 대한 weight 값을 보정하지 않고 RR 방식으로 각 VC에 할당을 해주기 때문에 네트워크의 변화에 신속하게 적응하지 못할 뿐 만 아니라 대역폭을 효율적으로 이용하지 못한다. 따라서 ABR 서비스를 위한 공평성 기준을 만족시키고 빠르게 적응할 수 있는 스케줄링 알고리즘이 필요하다. 본 논문에서는 ABR 서비스를 위한 6가지 공평성 기준들 중 MCR plus equal share와 Maximum of MCR or Max-Min share 두 기준을 만족시키는 스케줄링 알고리즘을 제안한다. 제안된 알고리즘이 공평성 기준을 만족시키고, 네트워크 자원을 각 ABR VC들에게 신속하고 공평하게 할당해 주는지를 컴퓨터 시뮬레이션을 통하여 WRR, Nabeshima et al 알고리즘들과 비교, 분석한다.

• International Journal of Safety
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• 제7권2호
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• pp.7-11
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• 2008

In this paper, a Magnetic Core Reactor (MCR) which forms a part of the DC reactor type three-phase high-Tc superconducting fault current limiter (SFCL) has been developed. This SFCL is more economical than other types with three coils since it uses only one high-Tc superconducting (HTS) coil. When DC reactor type three-phase high-Tc SFCL is developed using just one coil, fewer power electronic devices and shorter HTS wire are needed. The SFCL proposed in this paper needs a power-linking device to connect the SFCL to the power system. The design concept for this device was sprang from the fact that the magnetic energy could be changed into the electrical energy and vice versa. Ferromagnetic material is used as a path of magnetic flux. When high-Tc superconducting DC reactor is separated from the power system by using SCRs, this device also limits fault current until the circuit breaker is opened. The device mentioned above was named Magnetic Core Reactor (MCR). MCR was designed to minimize the voltage drop and total losses. Majority of the design parameters was tuned through experiments with the design prototype. In the experiment, the current density of winding conductor was found to be $1.3\;A/mm^2$, voltage drop across MCR was 20 V and total losses on normal state was 1.3 kW.

• Communications for Statistical Applications and Methods
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• 제26권1호
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• pp.35-46
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• 2019

Multivariate Confidence Region (MCR) cannot be used to obtain the confidence region of the mean vector of multivariate data when the normality assumption is not satisfied; however, the Quantile Confidence Region (QCR) could be used with a Multivariate Quantile Vector in these cases. The coverage rate of the QCR is better than MCR; however, it has a disadvantage because the QCR has a wide shape when the probability density function follows a bimodal form. In this study, we propose a Quantile Confidence Region using the Highest density (QCRHD) method with the Highest Density Region (HDR). The coverage rate of QCRHD was superior to MCR, but is found to be similar to QCR. The QCRHD is constructed as one region similar to QCR when the distance of the mean vector is close. When the distance of the mean vector is far, the QCR has one wide region, but the QCRHD has two smaller regions. Based on these features, it is found that the QCRHD can overcome the disadvantages of the QCR, which may have a wide shape.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.225-229
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• 2005

To investigate impediments to plasmid transformation in Brevibacterium flavum BF4 and B. lactofermentum BL1, cell surface barriers were determined by measuring growth inhibition whilst enzymatic barriers were determined by comparing DNA methylation properties. B. lactofermentum was more sensitive to growth inhibition by glycine than B. flavum. Release of cellular proteins during sonication was more rapid for B. lactofermentum than for B. flavum. Plasmid DNA (pCSL 17) isolated from B. flavum transformed recipient $McrBC^+$ strains of Escherichia coli with lower efficiency than $McrBC^-$. McrBC digestion of this DNA confirmed that B. flavum contain methylated cytidines in the target sequence of McrBc sequences but B. lactofermentum contained a different methylation pattern. DNA derived from the B. lactofermentum transformed recipient $EcoKR^+$ strains of E. coli with lower efficiency than $EcoKR^-$, indicating the presence of methylated adenosines in the target sequence of EcoK sequences. The present data describe the differences in the physical and enzymatic barriers between two species of corynebacteria and also provide some insight into the successful foreign gene expression in corynebacteria.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2003년도 춘계학술발표강연 및 논문개요집
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• pp.219-219
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• 2003

HVOF(High Velocity Oxygen Fuel)법을 사용한 MCrAlY(M=Ni, Co, Fe)계 열차폐 코팅(thermal barrier coating)은 열기관 내부의 극심한 환경 부하에 대해 구조물 표면에 열적, 화학적 장벽을 형성함으로써 구조물의 내구성을 향상시킨다 이와 동시에 열차폐 효과는 구조물의 온도상승 없이 내부 가동 온도를 높일 수 있게 함으로써 열효율을 상승시키고 연료 효율을 높여 가동비용 절감을 이룰 수 있는 동시에 고 연소를 통한 오염원의 배출을 감소시킬 수 있다. 본 연구에서는 $H_2O$$_2$=5:1 분위기 하에서 HVOF법을 사용하여 Hastelloy-X 기판위에 125$\mu\textrm{m}$의 두께로 다음 5종류의 (Ni, Co, Cr)계 MCrAlY 코팅을 용사시켰다. 준비된 (Ni, Co)-Cr-Al-(Y, Ta, Re), (Ni, Co)-Cr-Al-(Y, Re), (Ni, Co)-Cr-Al-(Y, Ta), (Ni, Co)-Cr-Al-Y, (Ni,Co)-Cr-Al-Ir 코팅시편에 대한 산화성질을 조사하기 위해 대기 중 1000, 1100, 120$0^{\circ}C$에서 50, 100, 150, 200시간 등온실험(Isothermal oxidation)을 실시하였고, XRD, SEM/EDS, EPMA를 이용하여 생성된 산화막과 코팅 시편의 조직 변화를 조사하였다. 산화온도와 산화시간이 증가할수록 산화막의 박리가 많이 발생하였으며, 분석 결과 미세하게 분포된 a-Al$_2$O$_3$ 입자, NiCr$_2$O$_4$스피넬 상, 미세한 Cr$_2$O$_3$가 관찰되었고, 코팅 조성 변화에 따라 형성되는 이들 산화물의 존재비가 달라졌으며, 산화온도가 높아질수록 산화속도가 가속화되었다.