• Title/Summary/Keyword: maximum cell density

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Characteristics of Deformation and Shear Strength of a Sandy Soil Deposited on the SAEMANKEUM Sea (새만금지역 해상에 퇴적된 사질토의 변형 및 전단강도 특성)

  • Lee, Kang-Il;Ju, Jae-Woo;Lee, Jin-Soo;Choi, Jong-Pyo
    • Journal of the Korean Geosynthetics Society
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    • v.9 no.3
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    • pp.29-37
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    • 2010
  • This study aims at evaluating the engineering properties of very poor graded fine sands deposited on the sea. Using materials sampled at SAEMANKEUM area, a series of rowe cell consolidation tests and triaxial compression tests are conducted in order to evaluate the characteristics of deformation and shear strength by the relative density. Prior to those tests, a maximum and a minimum relative densities are obtained. As a result, it appears that the minimum void ratio is 0.88, and the maximum compactible relative density is about 71%. In addition, internal frictional angle appears to increase linearly with an increase of the relative density which is similar to that of the port KUNJANG.

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Improved structures of stainless steel current collector increase power generation of microbial fuel cells by decreasing cathodic charge transfer impedance

  • Nam, Taehui;Son, Sunghoon;Kim, Eojn;Tran, Huong Viet Hoa;Koo, Bonyoung;Chai, Hyungwon;Kim, Junhyuk;Pandit, Soumya;Gurung, Anup;Oh, Sang-Eun;Kim, Eun Jung;Choi, Yonghoon;Jung, Sokhee P.
    • Environmental Engineering Research
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    • v.23 no.4
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    • pp.383-389
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    • 2018
  • Microbial fuel cell (MFC) is an innovative environmental and energy system that converts organic wastewater into electrical energy. For practical implementation of MFC as a wastewater treatment process, a number of limitations need to be overcome. Improving cathodic performance is one of major challenges, and introduction of a current collector can be an easy and practical solution. In this study, three types of current collectors made of stainless steel (SS) were tested in a single-chamber cubic MFC. The three current collectors had different contact areas to the cathode (P $1.0cm^2$; PC $4.3cm^2$; PM $6.5cm^2$) and increasing the contacting area enhanced the power and current generations and coulombic and energy recoveries by mainly decreasing cathodic charge transfer impedance. Application of the SS mesh to the cathode (PM) improved maximum power density, optimum current density and maximum current density by 8.8%, 3.6% and 6.7%, respectively, comparing with P of no SS mesh. The SS mesh decreased cathodic polarization resistance by up to 16%, and cathodic charge transfer impedance by up to 39%, possibly because the SS mesh enhanced electron transport and oxygen reduction reaction. However, application of the SS mesh had little effect on ohmic impedance.

Effect of MEA fabrication on the performance degradation of DMFC (MEA 제조 방법에 따른 직접 메탄올 연료전지의 성능저하 현상 평가)

  • Cho, Yoon-Hwan;Cho, Yong-Hun;Park, Hyun-Seo;Won, Ho-Youn;Sung, Yung-Eun
    • New & Renewable Energy
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    • v.3 no.1 s.9
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    • pp.60-67
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    • 2007
  • Catalyst coated membrane [CCM] type and catalyst coated substrate [CCS] type of membrane electrode assembly [MEA] were manufactured and evaluated their performance. Degradation test were conducted to find the difference of long term stability in two types of MEA and the factor for performance degradation problem occurred. Performance degradation test of single cell in two different types of MEA were carried out when current density was $200mA/cm^{2}$. The degradation test had proceeded for 230 hours and performance degradation was checked by I-V curve and impedance measurement at regular intervals. Also, MEA before/after operation and changes of catalyst layer were characterized by SEM, TEM, and XRD. Maximum power density of CCM type was higher than that of CCS type. Meanwhile, an increase of particle size of catalyst and an increase of impedance resistance after long term operation were observed. In the case of using CCM type MEA, the performance was deteriorated 38% of initial performance. In the case of using CCS type MEA, the performance was deteriorated 43% of initial performance. In consideration of difference of initial performance, performance of CCM type is higher than that of CCS type but both types had similar problems during degradation test.

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Enhanced reutilization value of shrimp-shell waste via fed-batch biodegradation with higher production of reducing sugar, antioxidant, and DNA protective compounds

  • Rashid, Harun Ar;Jung, Hyun Yi;Kim, Joong Kyun
    • Fisheries and Aquatic Sciences
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    • v.21 no.10
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    • pp.33.1-33.11
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    • 2018
  • As a process for commercial application, production of reducing sugar, antioxidant, and DNA protective compounds from shrimp-shell powder was investigated in a fed-batch biodegradation using Bacillus cereus EW5. The fed-batch biodegradation was operated in a 5-L bioreactor for 96 h according to three times pulse-feeding strategy. On the basis of the equal working volume (3 L), the fed-batch biodegradation showed a better production of the target compounds than the batch biodegradation, with higher cell density and shortened biodegradation period. The maximum values of the target compounds were 0.297 mg/mL of reducing sugar, 92.35% DPPH radical scavenging activity, 98.16% ABTS radical scavenging activity, and 1.55 reducing power at $A_{700}$, which were approximately 12.1, 3.4, 5.2, and 8.4% enhanced, respectively, compared with those obtained from the batch biodegradation. The fed-batch culture supernatant also showed the enhanced DNA damage inhibition activity than the batch culture supernatant. As a result, the fed-batch biodegradation accompanied by high cell density could produce more useful compounds, enabling an increase in the reutilization value of shrimp-shell waste.

An Electrical Repair Circuit for Yield Increment of High Density Memory (고집적 메모리의 yield 개선을 위한 전기적 구제회로)

  • 김필중;김종빈
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.13 no.4
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    • pp.273-279
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    • 2000
  • Electrical repair method which has replaced laser repair method can replace defective cell by redundancy’s in the redundancy scheme of conventional high density memory. This electrical repair circuit consists of the antifuse program/read/latch circuits, a clock generator a negative voltage generator a power-up pulse circuit a special address mux and etc. The measured program voltage of made antifuses was 7.2~7.5V and the resistance of programmed antifuses was below 500 Ω. The period of clock generator was about 30 ns. The output voltage of a negative voltage generator was about 4.3 V and the current capacity was maximum 825 $mutextrm{A}$. An antifuse was programmed using by the electric potential difference between supply-voltage (3.3 V) and output voltage generator. The output pulse width of a power-up pulse circuit was 30 ns ~ 1$mutextrm{s}$ with the variation of power-up time. The programmed antifuse resistance required below 44 ㏀ from the simulation of antifuse program/read/latch circuit. Therefore the electrical repair circuit behaved safely and the yield of high densitymemory will be increased by using the circuit.

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The electrochemical Characteristics on the Anode Material of Lithium Ion Secondary Batteries with Discharge Voltage (방전전압에 따른 리튬 이온 2차전지용 음극물질의 전기화학적 특성)

  • Park, Jong-Gwang;Han, Tae-Hui;Jeong, Dong-Cheol;Im, Seong-Hun;Han, Byeong-Seong
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.49 no.6
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    • pp.328-334
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    • 2000
  • A lithium ion secondary battery using carbon as a negative electrode has been developed. Further improvements to increase the cell capacity are expected by modifying the structure of the carbonaceous material. There are hopes for the development of large capacity lithium ion secondary batteries with long cycle, high energy density, high power density, and high energy efficiency. In the present paper, needle cokes from petroleum were examined as an anode of lithium ion secondary battery. Petroleum cokes, MCL(Molten Caustic Leaching) treated in Korea Institute Energy Research, were carbonized at various temperatures of 0, 500, 700, $19700^{\circ}C$ at heating rate of $2^{\circ}C$/min for lh. The electrolyte was used lM liPF6 EC/DEC (1:1). The voltage range of charge & discharge was 0.0V(0.05V) ~ 2.0V. The treated petroleum coke at $700^{\circ}C$ had an initial capacity over 560mAh.g which beyond the theoretical maximum capacity, 372mAh/g for LiC6. This phenomena suggests that carbon materials with disordered structure had higher cell capacity than that the graphitic carbon materials.

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The Effect of Glutamine on Production of Tissue-type Plasminogen Activator from Recombinant Human Melanoma Cells in Glutamine-limited Fed-batch Cultivation

  • Kim, Hyun-Goo;Kim, Tae-Ho;Kim, Dae-Seok;Park, Kyung-Yu;Park, jin-seo;Ahn, Chol;Lee, Jin-Ha;Lee, Hyeon-Yong
    • Journal of Microbiology and Biotechnology
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    • v.6 no.4
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    • pp.295-298
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    • 1996
  • Under glutamine-limited condition, $2\times10^6$ (viable cells/ml) of maximum cell density and 13.5 ($\mu g$/ml) of tissue-type Plasminogen Activators (tPA) production were maintained by spike feeding fresh medium in fed-batch cultivation of human recombinant melanoma cells. It showed that tPA production was much seriously affected than cell growth according to initial glutamine concentrations. Above 3.4 (mmol/I) of glutamine concentration both cell growth and tPA production were not much affected by increasing initial glutamine concentration. Glutamine depleted situation was occurred at latter periods of batch and fed-batch cultivations below 5.4 (mmole/I) of initial glutamine concentration. It also showed that maximum glutamine consumption and ammonia evolution rates were closely related to initial glutamine concentrations. Maximum specific tPA production rate was estimated as $8.1\times19^{-6}$ ($\mu g$/cells/h) at 3.4(mmol/I) of glutamine concentration, which is higher than that from other batch and fed-batch processes.

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Effect of the Organic and Nitrogen Removal and Electricity Production on Changing the External Resistor and the Inflow Loading in the Biocathode Microbial Fuel Cell (생물환원전극 미생물연료전지에서 외부저항 및 유입부하에 따른 유기물 및 질소 제거와 전기생산에 미치는 영향)

  • Kim, Jiyeon;Kim, Byunggoon;Kim, Hongsuck;Yun, Zuwhan
    • Journal of Korean Society on Water Environment
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    • v.31 no.5
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    • pp.556-562
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    • 2015
  • In order to remove the organic substances and the nitrate-nitrogen contained in wastewater, some researchers have studied the simultaneous removal of organics and nitrogen by using different biocathode microbial fuel cells (MFCs). The operating conditions for removing the contaminants in the MFCs are the external resistances, HRTs, the concentration of the influent wastewater, and other factors. This study aimed to determine the effect of the external resistors and organic loading rates, from the changing HRT, on the removal of the organics and nitrogen and on the production of electric power using the Denitrification Biocathode - Microbial Fuel Cell (DNB-MFC). As regards the results of the study, the removal efficiencies of $SCOD_{Cr}$ did not show any difference, but the nitrate-nitrogen removal efficiencies were increased by decreasing the external resistance. The maximum denitrification rate achieved was $129.2{\pm}13.54g\;NO_3{^-}-N/m^3/d$ in the external resistance $1{\Omega}$, and the maximum power density was $3,279mW/m^3$ in $10{\Omega}$. When the DNB-MFC was operated with increasing influent organic and nitrate loading by reducing the HRTs, the $NO_3{^-}-N$ removal efficiencies were increased linearly, and the maximum nitrate removal rate was $1,586g\;NO^3{^-}-N/m^3/d$ at HRT 0.6 h.

Effects of Pluronic F-68 on Cell Growth of Digitalis lanata in Aqueous Two-Phase Systems

  • LEE , SANG-YOON;KIM, DONG-IL
    • Journal of Microbiology and Biotechnology
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    • v.14 no.6
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    • pp.1129-1133
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    • 2004
  • The effects of Pluronic F-68, a non-ionic surfactant, on the growth and physical characteristics of Digitalis lanata suspension cultures were investigated in aqueous two-phase systems (ATPSs) composed of $4.5\%$ polyethylene glycol (PEG) 20,000 and $2.8\%$ crude dextran. In the range of 0.1-10.0 g $1^{-1}$, Pluronic F-68 enhanced the maximum cell density in a medium with ATPSs, even though Pluronic F-68 did not affect cell growth in a normal growth medium. In terms of physical properties of ATPSs with cell suspension cultures, 0.2 g $1^{-1}$ of Pluronic F-68 reduced viscosity by up to $40\%$, while 0.1 g $1^{-1}$ of Pluronic F-68 significantly enhanced the oxygen transfer rate. In addition, we successfully performed aqueous two-phase cultivation in a 5-1 stirred tank bioreactor with 0.5 g $1^{-1}$ of Pluronic F-68, and discovered that cell growth in ATPSs was similar to that in normal growth medium.

The Development of Serum Free Medium for the Economic Production of scu-PA from HEK Cells (인간 신장세포로부터 scu-PA의 경제적 생산을 위한 무혈청 배지의 개발)

  • 김현규;김현구
    • KSBB Journal
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    • v.9 no.5
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    • pp.518-524
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    • 1994
  • Serum free medium was developed to cultivate Human Embryonic Kidney cells with Cytodex III microcarriers. The cells normally attached and spreaded on the microcarriers in serum free medium, and grew well by bead to bead processes. 85% of attachment yield was obtained on Cytodex III in this medium, compared to about 93% in 1% serum containing medium. About 90% of the attached HEK cells spreaded after 6 hours of post-attachment periods on the surface of microcarrier. Maximum cell density and scu-PA concentration in a serum free medium were $9.1{\times}10^5$ cells/ml and 1790 IU/ml, respectively, with fed-batch cultivation. Maximum cell density and scu-PA concentration in this medium with perfusion cultivation were $2.5{\times}10^6$ cells/ml and 1820 IU/ml, respectively. The conversion of single chain urokinase type plasminogen activator (scu-PA) into two chain type plasminogen activator (tc-PA) was less than 5% in a serum free medium compared to about 10% in 0.5% serum containing medium.

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