• Applied Biological Chemistry
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• v.37 no.1
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• pp.43-48
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• 1994

A basic inducible protein, ICG, containing chitinase and ${\beta}-1,3-glucanase$ activity concomittantly was purified from cell suspension culture media of rice after the treatment of chitooligosaccharides. The isolated ICG enzyme gave a single band on native and SDS polyacrylamide gel electrophoresis and its molecular weight was estimated to be 52.53 kd. The optimal temperature and optimal pH of both enzyme activities in ICG were $60^{\circ}C$, pH 6.0 for chitinase activity and $37^{\circ}C$, pH 4.0 for ${\beta}-1,3-glucanase$ activity. $K_M$ and $V_{max}$ values for chitinase were 0.474 mM. 2.997 nM/min., and those for ${\beta}-1,3-glucanase$ were 1.004 mM 0.739 nM/min. respectively. TLC analysis of the chitooligosaccharide hydrolysates with ICG enzyme indicated that ICG acts as endochitinase.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.6-11
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• 1998

Bean sprout is one of the traditional vegetable in Korea and produced by sprouting soybean (Glycine max L.) cultivars. This experiments was conducted to investigate the ecological characteristics in collected small grain soybean lines from Chungnam province region. Thirty varieties were used in this study. Seed coat color of collected small soybean lines showed eight different types. Major seed coat color type was yellow as 8 lines of total 35 lines. The stem height, number of nodes and number of branches averaged 58.5 cm, 16.5 nodes and 11.8 branches, respectively. The average number of main stem pods and number of branching pod were appeared 43.1 and 62.7 respectively. One hundred seed weight averaged 9.9g which was lower than 10~12g of sprouting soybean cultivars as breeding goal of Korea. The average of node width showed 13.2cm and long stem open types and short stem close types were classified 1 line and 14 lines. The flower dates of collecting small soybean lines were between 8 Aug. and 13 Aug. and flowering duration was 74~85 days. The date of maturity were between 12 Sep. and 12 Oct., and maturity duration showed great variation among cultivated soybean lines.

• Journal of agricultural medicine and community health
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• v.35 no.2
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• pp.165-176
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• 2010

Objectives: This study is to identify the effect of detraining on lipoprotein and body composition after 8 week calorie restriction and different level of aerobic exercise among obese middle-aged women. Methods: The 30 middle-aged women whose body fat were over 30% were randomly allocated into three groups of control, exercise group of 40% $VO_2$max level, and exercise group of 60% $VO_2$max level. All the participants were taught to record their calorie intake and restrict daily 500kcal intake. After eight-week exercise and daily 500kcal restriction, their body compositions and blood lipid levels were measured again. Results: The weight, BMI, body fat, total cholesterol, triglyceride, and LDL-cholesterol levels showed significant decreases after eight-week exercise and calorie restriction in the two exercise groups. However, detraining tended to increase them again after detraining of 4 weeks, and even more after 8 weeks. Conclusions: Detrainging after calorie restriction and exercise tended to increase levels of indices for body composition and lipoprotein among obese middle-aged women.

• Applied Biological Chemistry
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• v.18 no.4
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• pp.183-187
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• 1975

The content of total and individual anthocyanins in the wild vines (Vitis amurensis Ruprecht), which were wildly grown in the mountain area of Korea, has been carried out. The pigments were extracted with 0.1% hydrochloric acid in methanol from the wild vines and the content of the total anthocyanin in the extract was determined spectrophotometrically at 538nm $({\lambda}max.)$. Individual anthocyanins in the extract were separated by paper chromatography and eluted with 0.1% HCl in methanol. The optical densities of the individual pigment solutions were determined at each absorption maxima of the pigments. The content of total anthocyanins in wild vines was 3.95mg per 1g fresh weight. The content of individual pigments were as follows: 12.5% delphinidin 3-monoglucoside. 3,5 % petunidin 3-monoglucoside, 2.1% cyanidin 3-monoglucoside, 10.1% delphinidin 3,5-diglucoside, 4.3% malvidin 3-monoglucoside, 8.3% petunidin 3,5-diglucoside, 4.1% cyanidin 3,5-diglucoside like and 55.1% malvidin 3,5-diglucoside. It was found that the most abundant pigment in wild vines studied was malvidin-3,5-diglucoside.

• Korean Journal of Microbiology
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• v.36 no.1
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• pp.26-32
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• 2000

Catechol 2,3-dioxygenase was purified from recombinant strain E. coli CNU312 carrying the tomB gene which was cloned from toluene-degrading Burkholderia cepacia G4. The purification of this enzyme was performed by acetone precipitation, Sephadex G-75 chromatography, electrophoresis and electro-elution. The molecular weight of native enzyme was about 140.4 kDa and its subunit was estimated to be 35 kDa by SDS-PAGE. It means that this enzyme consists of four identical subunits. This enzyme was specifically active to catechol, and$K_(m)$ value and $V_(max)$value of this enzyme were 372.6 $\mu$M and 39.27 U/mg. This enzyme was weakly active to 3-methylcatechol, 4-methylcatechol, and 4-chlorocatechol, but rarely active to 2,3-DHBP. The optimal pH and temperature of the enzyme were pH 8.0 and $40^{\circ}C$. The enzyme was inhibited by $Co^(2+)$, $Mn^(2+)$, $Zn^(2+)$, $Fe^(2+)$, $Fe^(3+)$, and $Cu^(2+)$ ions, and was inactivated by adding the reagents such as N-bromosuccinimide, and $\rho$-diazobenzene sulfonic acid. The activity of catechol 2,3-dioxygenase was not stabilized by 10% concentration of organic solvents such as acetone, ethanol, isopropyl alcohol, ethyl acetate, and acetic acid, and by reducing agents such as 2-mercaptoethanol, dithiothreitol, and ascorbic acid. The enzyme was inactivated by the oxidizing agent $H_(2)$$O_(2)$, and by chelators such as EDTA, and ο-phenanthroline.

• Journal of Adhesion and Interface
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• v.10 no.4
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• pp.191-198
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• 2009

UV-curable aliphatic epoxy acrylates were prepared by the reaction of glycerol diglycidyl ether (GDE) with 2-carboxyethyl acrylate (2-CEA) or 2-hydroxyethyl acrylate (2-HEA). The structures of the epoxy acrylates were characterized by FT-IR, $^1H$-NMR, and $^{13}C$-NMR and the yield was obtained by prep-LC. The UV- and the thermal-curing behaviors of the product were investigated using photo-DSC and DSC, respectively. The reactivity of 2-CEA was higher than 2-HEA and the yield of the product (GEA-C) which was prepared using 2-CEA was about 83%. The maximum UV-curing time ($T_{max}$) of the GEA-C contained non-reactive components and by-product was about 10 seconds. The GEA-C showed low color difference (${\Delta}E^*$), low viscosity, and good thermal stability - its value was 2.51, 192 cps, and $299^{\circ}C$ (at 5% weight loss), respectively. The activation energies ($E_a$) of thermal-curing reaction calculated from Kissinger and Ozawa-Flynn-Wall method were 91~92 kJ/mol.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.5
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• pp.637-644
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• 1998

[ $\beta$ ]-Galactosidase was extracted from the internal organ of sea urchin, Hemicentrotus pulcherrimus The enzyme was purified 384.6-fold over the crude extract by the sequential chromatographic methods including DEAE-Sephadex A-25, CM-Cellulose, and Con A-Sepharose 4B affinity chromatography with a recovery $1.26\%$. The molecular weight of the purified enzyme was estimated approximately 94 kDa as monomeric term by SDS-PAGE and Sephadex G-150 gel chromatography. The maximum enzymatic activity was observed at pH 3.0 and $50^{\circ}C$ but the one was stable over the ph range or 3.0$\~$5.0 and below $37^{\circ}C$. The $K_m$ and $V_{max}$ values against PNPG (P-nitrophenyl $\beta$-D-galactopyranoside) were 15.0 mM and 214 $\mu$mole/min per mg protein, respectively. The enzymatic activity was activated by $Ba^{2+}$, but significantly inhibited by $DEP,\;Hg^{2+},\;Sn^{2+}$ and galactose.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.1
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• pp.54-59
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• 1992

The characteristics of endogenous and exogenous ${\beta}-galactosidase\;({\beta}-Galase)$ produced from L. acidophilus were investigated as one of the serial studies on the fermentation of Chinese cabbage kimchi using L. acidophilus. Apparent molecular weight of endogenous and exogenous of the ${\beta}-Galase$ were investigated to be 550,000 and 740,000 daltons by the method of gel filtration and Km values of the both enzymes were 1.67mg/ml, 1.33mg/ml and $V_{max}$ were $8.5\;{\mu}\;mol/mg/30min.$, $2.65\;{\mu}\;mol/ml/30min.$, respectively. The optimum pH of the enzymes were 7 and 8, respectively. The optimum temperatures and salt concentrations of the both enzyme were the same and appeared to $30^{\circ}C$and$4{\sim}5%$, respectively. The activities of the endogenous and exogenous ${\beta}-Galase$ were decreased by increasing of temperature from $60^{\circ}C$to$90^{\circ}C$ and the decreasing rate of the enzyme activities on the processing of the heating times showed high at first 2 minutes of heating.

• KSBB Journal
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• v.6 no.4
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• pp.327-336
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• 1991

A study on the optimum hydrolysis conditions of fish skin through the aid of enzymes and the development of a natural seasoning using the hydrolysate has been carried out for the effective utilization of fish skin. Using the "pH-drop" techniques the collagenase and pronase were identified as most suitable for this purpose. The $K_m$ and $V_{max}$ values of pronase were 1.82 mgN/ml and 0.06 mgN/mL/min, respectively. The hydrolysis conditions of the cod skin for the pronase were as follows: reaction temperature, $50^{\circ}C$; reaction time, 3hrs; pH 6; enzyme concentration, 0.03%. The degree of hydrolysis at these conditions was 76.8%. But after hydrolyzing cod skin with collagenase for 1hr, when the pronase was treated, the degree of hydrolysis was 83.13%. The molecular weight of the hydrolysate was 8,000 daltons. Among the amino acids in the hydrolysate, glycine(27.95%), glutamic acid(10.94%), proline(7.39%), aspartic acid(9.47%) and serine(7.39%) were responsible for 64.23% of the total amino acids. But valine, methionine, isoleucine, leucine, phenylalanine and histidine having a bitter taste were only 13.05%. From the results of the sensory evaluation, the imitation sauce which was made of 20% fermented soy sauce prepared from the hydrolysate was at least similar to the traditional soybean sauce in product quality. The complex seasoning containing 31.7% of the hydrolysate was nearly equal to complex seasonings on the market, too.

• Korean Journal of Environment and Ecology
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• v.28 no.6
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• pp.634-641
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• 2014

In order to elucidate the ecological and phenological responses of bean under global warming situation, we investigated leafing and flowering phenomena and growth traits of Baktae and Seomoktae in control plot (ambient $CO_2$ concentration and temperature) and warmed plot (elevated $CO_2$ concentration and increased temperature than control) in green house. Average $CO_2$ concentration and temperature were set 540 ppm in treatment and higher $2.2^{\circ}C$ in treatment than control. The appearance of leaf unfolding and the initiation of blooming and fruit maturing in Seomoktae were late in treatment than control. In case of Baktae, the number of total pods and seeds per plant in treatment was fewer than that in control. For Seomoktae, the number of total pods per plant and weight of total seeds per plant were decreased in treatment than in control. Thus, the number of pods and seeds of Baktae declined and the production of Seomoktae was decreased under global warming condition. This result indicates that site selection and crop yield for cultivating of the bean may be changed into new area due to global warming condition.