• The Korean Journal of Malacology
• /
• v.4 no.1
• /
• pp.30-41
• /
• 1988

The gonadal development, the annual reproductive cycle and the first sexual maturity of surf clam, Mactra veneriformis Reeve were studied histologically. Speciemens were monthly collected at the intertidal zone of Naechodo, Chollabuk-do, Korea, for one year from March 1986 to February 1987. Sexuality of the clam is dioecious. The gonads were located between the subregion of mid-intestinal gland in the visceral cavity and the reticular connetive tissues of the foot, The ovary is composed of a number of ovarian sacs, and the testis comprise several testiculat lobules. The undifferentiated mesenchymal tissues and eosinophilic granular cells function as nutritive cells in the early stage. The ripe eggs were about 50-60${\mu}{\textrm}{m}$ in diameter, and they were wurroundedby the gelatinous membranes. The spawing period was from early June to September the main spawning occurred beetween July and August when the water temperature reached above 24$^{\circ}C$. The annual reproductive cycle of this species could be classified into five successive stages: multiplicative(January to March), growing(March to May), mature(April to August), spent(June to September), degenerative and resting(September to February). The monthly changes of fatness coefficient closely correlated with the annual reproductive cycle. Percentages of the first sexual maturity of female and male clams were over 50% among those individals ranging from 2.1 To 2.5cm, and 100% in those over 2.6cm in shell length.

• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.1
• /
• pp.369-372
• /
• 2016

Background: Acute myeloid leukemia is an acquired clonal heterogeneous stem cell disorder. Hence, various parameters are sought out to categorize this disease into subtypes, so that as a consequence specific treatment modalities can be offered. Conventionally, the practically used method for classification utilizes French American British (FAB) criteria based on morphology and cytochemistry. The aim of present study was to determine the current spectrum of AML sub types in patients in Karachi. Materials and Methods: This single centre cross sectional study was conducted at Liaquat National Hospital, Karachi, extending from January 2010 to December 2014. Data were retrieved from archives were analyzed with SPSS version 22. Results: A total of 125 patients were diagnosed at our institution with de novo AML during five years period, 76 males and 49 females. Median age was 34.5 years. AML-M1 was the predominant FAB subtype (23.2%) followed by M2 (18.4%), M3 and M4 (16% each), M0 (14.4%), M5 (7.2%), M6 (3.2%) and M7 (1.6%). Conclusions: AML in Pakistani patients is seen in a relatively young population. The most common FAB subtype observed in our study was acute myeloblastic leukemia, without maturation (M1).

• Korean Journal Plant Pathology
• /
• v.13 no.2
• /
• pp.105-112
• /
• 1997

Didymella bryoniae, gummy stem blight fungus of cucurbits, has been known not to produce its pycnidium in vitro without irradiation. Various methods for producing pycnidiospores of the fungus as an inoculum have been used. However, those methods have not been verified in terms of efficiency of the productivity, activity and synchronous maturation of the inoculum. Therefore, a pycnidiospore production method in vitro that is highly reliable and reproducible has to be developed to obtain a large amount of inoculum for screening disease resistant varieties or effective fungicides. Here we standardized a mass-production technique for pycnidiospores of D. bryoniae in vitro by comprehensively finding the optimal conditions such as kinds and thickness of cultural medium, growing temperature, and quality and duration of irradiation as well as examining the activity and pathogenicity of the pycnidiospores reproduced. In brief, mycelial colony on the PDA plate was cultured at 26$^{\circ}C$ for 2 days under the darkness, and then the plate was irradiated under the UV light (12 hr/a day) for 2~3 days at the same temperature(26$^{\circ}C$). Two days after UV irradiation, a great number of pycnidia was simultaneously formed. This plate was subjected to darkness again for 4~5 days to mature pycnidiospores. We could obtain a large amount of inoculum that is synchronously matured in a short period of time through the above procedures.

• Journal of Embryo Transfer
• /
• v.25 no.2
• /
• pp.93-96
• /
• 2010

The isolation of preantral follicles from the ovaries of bovine was performed under mechanical and enzymatic methods. A significant increase in the total number of follicles retrieved was detected when tissue chopper was used. Micro-dissection could supply good quality, larger sized follicles (400 to $700{\mu}m$) but with the lowest yield ($9.0{\pm}1.0$). The isolated preantral and early antral follicles were cultured for 14 days. Follicles isolated by the mechanical method had a greater growth during a culture period than follicles collected enzymatically. Morphologically normal bovine oocytes from early antral follicles after 14 days culture were 59.6% after culture and after 24 h of maturation culture, 12.9% of in vitro-grown oocytes reached the second metaphase. In conclusion, this study showed that mechanical method can be used effectively to isolate intact preantral follicles from bovine ovaries.

• The Korean Journal of Zoology
• /
• v.26 no.2
• /
• pp.83-93
• /
• 1983

Vitellogenin was purified from the haemolymph of female pupae during egg formation of Bombyx mori using DEAE-cellulose column and its quantitative change in various organs with age ws examined by electrophoresis and immunodiffusion. Vitellogenin is distributed in the haemolymph, fat body, and over the egg maturation and especially maintainsa constant level in the haemolymph until just before emergence, indicating that vitellogenin in released into the haemolymph at the same amount as it is taken up by oocytes during pupal period. Immunologically vitellogenin was confirmed to be in the fat body until 7 days after pupation and to undergo a drastic decline thereafter. Also, the interaction of anti-ovary proteins with haemolymph proteins showed at least 3 homogeneous proteins, indicating that other proteins as well as vitellogenin are involved in egg formation.

• Microbiology and Biotechnology Letters
• /
• v.5 no.1
• /
• pp.5-12
• /
• 1977

As a basic study for the application of the spore-tearing lactic acid bacteria to foods, the effects of the sporulating conditions on the growth and sporogenesis were studied were observed. The results obtained are as follow. 1. All carbohydrates added to sporulation media except dextrin decreased the sporulation rate and the thermal resistance of spores. Dextrin stimulated the growth, however, there in no effect on the thermal resistance. 2. As nitrogen source, the protein hydrolysates such as peptone, casamino acid were effective to obtain were spores of the increased thermal resistance. 3. Ca$\^$＋＋/, Mn$\^$＋＋/ of the metal ions added to casamino acid containing medium validly increased the total growth, sporulation rate and thermal resistance. Its optimum concentration was 40 ppm each. 4. Biotin of vitamines had an effect on the total growth, sporulation and thermal resistance of spores. Its optimum concentration was 30${\gamma}$/ml. 5. The resistant spores required the adequate maturation period, more than 36 hours, sufficient aeration. and optimum temperature, 37∼45$^{\circ}C$.

• Journal of Fisheries and Marine Sciences Education
• /
• v.24 no.6
• /
• pp.854-861
• /
• 2012

To investigate the role of water temperatures and photoperidos as environmental cues regulating reproductive rhythm in banded catfish Pseudobagrus fulvidraco, rearing experiments were conducted using sveral rearing regimes conbined with changes in photoperiods and water temperatures during growing and spawning periods. GSI of $23^{\circ}C$ was significantly higher than $18^{\circ}C$ in female, but 9 light of 18 and $23^{\circ}C$ were no difference in male. In case of estradiol, $18^{\circ}C$ and 15 L was higher than other experintal precinct after 20 days. But, end of the experiment, all of experimental precinct were no difference. Testosteron of female was no difference in $18^{\circ}C$-9 and 15 L after 20 days. Testosteron of male was no difference in $18^{\circ}C$-9 L. In case of 11-KT, control level was $0.39{\pm}0.03$ ng/mL and $18^{\circ}C$-9 L was no significantly difference after 20 days. But, 15 L was higher than other experimental precinct.

• Korean Journal of Ichthyology
• /
• v.29 no.2
• /
• pp.109-116
• /
• 2017

We investigated maturation and spawning of Miichthys miiuy in the south-western water of Korea, based on samples collected by stow net from August 2015 to December 2016. We analysed monthly changes in maturity stage, gonadosomatic index (GSI), total length (TL) at 50% group maturity. The spawning period was August to October. We estimated the TL at 50% group maturity as 54.8 cm for females and 49.0 cm for males.

• Applied Microscopy
• /
• v.28 no.4
• /
• pp.539-549
• /
• 1998

The silk glands of the spiders are of several types. Among the several types of spider's silk glands, the principal fibers used in constructing the eggcase are products of the tubuliform glands (TBG), which are present only in females. Development of these glands parallels maturations of the ovaries and fat bodies. In order to understand the mechanism of eggcase-silk production, this paper has examined the fine structural changes of the TBG during the period of egg maturation in the garden spider, Argiope aurentia. Between the two kinds of secretory granules observed in the glandular epithelium of the mature TBG, the electron-dense granules which have paracrystalline structure are revealed to be the precursors of the eggcase silk fibers. During the production of eggcase, rapid release of the secretory product occurs at apical surface by the mechanism of apocrine secretion. Moreover, secondary lysosomes appear due to the rapid disorganization of cellular components during the eggcase formation. Examinations of formed fibers indicate a multicomponent internal structure, and electron micrographs reveal each fiber contains numerous electron lucent fibrils embedded in an amorphous electron dense matrix. The secretory precursors are produced as separated vesicles via well-oriented rER, and no Golgi complex has been found in the glandular epithelial cells.

• The Korea Journal of Herbology
• /
• v.38 no.2
• /
• pp.9-16
• /
• 2023

Objectives : Many people use natural soap instead of commercially available bar type general soap for skin care. They tend to prefer cp (cold process) type soaps that go through a sufficient maturation process in formulation. The purpose of this study was to investigate the efficacy of PCPS (placenta cp soap). Methods : The researcher selected 36 subjects, 28 as the experimental group and 8 as the control group. I asked participants to wash their face twice a day in the morning and evening, and the period was set for 6 weeks. The researcher had the people who were selected as the control group use a commercially available foam cleanser, meanwhile the experimental group use PCPS. They performed a skin test before the start of the test, and the change status was continuously investigated after 2 weeks, 4 weeks, and 6 weeks after using the soap. Results : In the case of U zone oil and pores, there was a significant decrease and in the case of skin tone, there was a significant increase in the group of using PCPS. In T zone oil change, there was no significant effect in both the experimental group and the control group. Conclusion : In the case of PCPS, it is recommended to use it in the effect of reducing the size of pores and increasing skin tone, but it can reduce u-zone oil content.