• The Korean Journal of Physiology and Pharmacology
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• 제27권1호
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• pp.113-125
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• 2023

It has been reported that stressful events in early life influence behavior in adulthood and are associated with different psychiatric disorders, such as major depression, post-traumatic stress disorder, bipolar disorder, and anxiety disorder. Maternal separation (MS) is a representative animal model for reproducing childhood stress. It is used as an animal model for depression, and has well-known effects, such as increasing anxiety behavior and causing abnormalities in the hypothalamic-pituitary-adrenal (HPA) axis. This study investigated the effect of MS on anxiety or aggression-like behavior and the number of GABAergic neurons in the hippocampus. Mice were separated from their dams for four hours per day for 19 d from postnatal day two. Elevated plus maze (EPM) test, resident-intruder (RI) test, and counted glutamic acid decarboxylase 67 (GAD67) or parvalbumin (PV) positive cells in the hippocampus were executed using immunohistochemistry. The maternal segregation group exhibited increased anxiety and aggression in the EPM test and the RI test. GAD67-positive neurons were increased in the hippocampal regions we observed: dentate gyrus (DG), CA3, CA1, subiculum, presubiculum, and parasubiculum. PV-positive neurons were increased in the DG, CA3, presubiculum, and parasubiculum. Consistent with behavioral changes, corticosterone was increased in the MS group, suggesting that the behavioral changes induced by MS were expressed through the effect on the HPA axis. Altogether, MS alters anxiety and aggression levels, possibly through alteration of cytoarchitecture and output of the ventral hippocampus that induces the dysfunction of the HPA axis.

• 아동학회지
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• 제37권1호
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• pp.17-36
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• 2016

Objective: Taking note of the fact that North Korean refugee children experience frequent separation from their mothers and long-term maternal deprivation during their childhood, this study examined the relationship between children's separation experiences and attachment. Methods: A total of 37 children aged from 5 to 9 were assessed on their attachment using the Separation Anxiety Test, and their mothers reported on their child's separation experiences. Results and Conclusion: The major findings of this study were as follows: first, the result of evaluating their attachment pattern showed North Korean refugee children had a high level of insecure attachment with a high tendency for avoidant attachment. This avoidant attachment tendency is probably due to growing up in a repressed emotional environment by frequently experiencing maternal separation in North Korea, China, and even after settling down in South Korea. Second, children's secure attachment level was higher if they did not experience separation from their mother, if their mother had a high level of education in South Korea, or if they lived with a big family.

• 아동학회지
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• 제27권4호
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• pp.247-263
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• 2006

Recruited from Korean-Chinese elementary schools in Shenyang and Harbin, China, 100 children living apart from their parents and experiencing non-maternal care provided information about the separation (reason and duration of parent-child separation, present location of parents, etc.), their adjustment to separation, and their perception of attachment with their caregivers. Results showed that although the separated children adjusted positively to parent-child separation in general, over 55% of them reported loneliness. Attachment with caregiver was the most significant influential variable on children's behavior problems. Duration of separation from father was related to children's hostility/aggression and hyperactivity/attention deficit problem, while duration of separation from mother influenced children's anxiety.

• Korean Journal of Acupuncture
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• 제21권3호
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• pp.77-88
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• 2004

Acupuncture has been shown to have therapeutic effects on many mental disorders such as depression, anxiety, and substance abuse in human and animal studies. Maternal separation (MS) is a risk factor in the development of mood-related disorders such as depression. This study investigated the effects of acupuncture during MS by evaluating locomotion as behavioral markers of depression and by observing changes in c-Fos expression in the limbic system (hippocampus, amygdala and cingulate cortex) to elucidate the mechanism of the therapeutic effect. The employed acupoint, Shinmun (HT7), has been clinically used to treat mental disorders. MS for 7 days beginning on postnatal day 14 induced a significant decrease in locomotion, while acupuncture treatment at acupoint HT7 resulted in a significant increase in locomotion. c-Fos immunoreactive cells in area dentate gyrus (DG) of the hippocampus were increased in the MS group, but significantly decreased in the acupuncture group. These findings suggest that acupuncture has an effect on the depression-like disorder caused by MS, possibly by modulating c-Fos expression in DC of the hippocampus.