• Korean Journal of Medicinal Crop Science
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• v.19 no.2
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• pp.111-116
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• 2011

The composition analysis and electron donating activity of 4 kinds of Korean wild sannamuls[Cacalia firma Komar. (CFK), Aster scaber Thunb. (AST), Ligularia fischeri (Ledeb.) Turcz. (LFT), Synurus palmatopinnatifidus (Makino) Kitam., SDN)] were investigated. The Korean edible mountainous vegetable was named a sannamul or chwinamul in Korea, generally. Most of them, the characterization of the each vegetable was reported, individually without comparative research. This study was compared the proximate compositions, mineral, dietary fiber and electron donating abilities on 2,2-diphenyl-1-picrylhydrazyl (DPPH) of sannamuls. In the chemical analysis of sannamuls, CFK was shown higher value in ash ($5.4{\pm}0.18%$), crude fat ($2.5 {\pm}0.14%$), carbohydrate ($8.1{\pm}0.21%$), potassium ($475.45{\pm}0.34mg$) and magnesium ($64.70{\pm}0.48mg$) than others. Each mineral of the 3 other samples was a similar value but phosphorus in LFT was contained $62.91{\pm}0.43%$. The compositions of total dietary fiber of the samples were listed CFK LFT SDN AST and the $RC_{50}$ Value on DPPH radicals, LFK CFT AST SDN. These results have susggest that CFK and LFT may be useful for a funtional material to food development.

• Korean Journal of Food Science and Technology
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• v.30 no.2
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• pp.397-406
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• 1998

Hen egg white lysozyme (HEWL) is very valuable as a natural preservative in food processing due to its selective bactericidal activity. HEWL which traditionally isolated by crystallization or freeze drying was simply separated from 13 different hen egg white (HEW) proteins by a single-step ultrafiltration. Freeze dried HEW (0.25%, w/v) dissolved in a citrate-phosphate buffer (pH 4.6) was ultrafiltered with a PM30 membrane under various operating conditions, by changing concentration, temperature, transmembrane pressure $({\triangle}P_T)$, and stirring speed. Optimum separation conditions were decided when maximal flux was obtained. Under the optimum separation conditions, the effect of membrane material and fouling on flux as time passed as well as lysozyme concentration, protein concentration, specific activity (SA) in the permeate were measured. Best separation conditions of HEWL with PM30 membrane were sample concentration 0.25%, temperature $35^{\circ}C$, ${\Delta}P_T\;30\;psi$, and stirring speed 300 rpm. During the first 12 min, the flux of YM30 was higher, but at the steady-state it was lower than that of PM30. The SA of the PM30 permeate was over 2 times higher in spite of the lysozyme and protein concentration being lower than that of YM30 permeate. The flux of 5 times used PM30 decreased 30% compared to a new PM30, but both had the same tendency in flux decrease when time passed. Both of them reached a steady-state after 35 min and remained at 70% of the initial flux. In the PM30 permeate, the lysozyme concentration and SA were 110 units/mL and 2,821 units/mg protein, respectively. Therefore, PM30 membrane separation was very effective for separation of antimicrobial lysozyme.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1520-1520
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• 2001

Food adulteration is a serious consumer fraud and a potentially dangerous practice. Regulatory authorities and food processors require a rapid, non-destructive test to accurately confirm authenticity in a range of food products and raw materials. Olive oil is prime target for adulteration either on the basis of the processing treatments used for its extraction (extra virgin vs virgin vs ordinary oil) or its geographical origin (e.g. Greek vs Italian vs Spanish). As part of an investigation into this problem, some preliminary work focused on the ability of near infrared spectroscopy to discriminate between virgin olive oils from separate regions of the Mediterranean i. e. Crete and the Peloponese. A total of 46 oils were collected: 18 originated in Crete and 28 in the Peloponese. Oils were stored in a temperature-controlled room at 2$0^{\circ}C$ prior to spectral collection at room temperature (15-18$^{\circ}C$). Samples (approximately 0.5$m\ell$) were placed in the centre of the quartz window in a camlock reflectance cell; the gold-plated baking plate was then gently placed into the cell against the glass so as to minimize the formation of air bubbles. The rear of the camlock cell was then screwed into place producing a sample thickness of 0.5mm. Spectra were recorded between 400 and 2498nm at 2nm intervals on a NIR Systems 6500 scanning monochromator. Spectral collection took place over 2-3 days. Data were analysed using both WINISI and The Unscrambler software to investigate the possibility of discriminating between the oils from Crete and the Peloponese. A number of data pre-treatments were used and discriminant models were developed using discriminant PLS (WINISI & Unscrambler) and SIMCA (Unscrambler). Despite the small number of samples involved, a satisfactory discrimination between these two oil types was achieved. Graphical examination of principal component scores for each oil type also holds out the possibility of separating oils from either Crete and the Peloponese on the basis of districts within each region. These preliminary data suggest the potential of near infrared spectroscopy to act as a screening technique for the confirmation of geographic origin of extra virgin olive oils. The sample presentation strategy adopted uses only small volumes of material and produces high quality spectra.

• Resources Recycling
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• v.19 no.3
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• pp.52-61
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• 2010

In general, the waste concrete is simply crushed and reused as a recycled aggregate at a low value application such as back filling material. It because that the quality of recycled aggregate is lower than one of natural aggregate due to the insufficient liberation of aggregate and cement mortar. So in this study, the liberation characteristics of liberation of aggregate and cement mortar is analyzed to investigate the limitation of conventional crushing stage at waste concrete processing circuit. In this process, thermal treatment method is evaluated for the enhancement of liberation. From test results, the preferential breakage along the grain boundary is not accomplished by the conventional crushers. It leads a low quality of recycled aggregate and a fracture of aggregate. To solve these problems, gentle breakage is used as a breakage mechanism to induce preferential breakage along the grain boundary. The recycled aggregate produced from the free fall test, which adopts a gentle breakage, shows a better liberation characteristics and a higher quality.

• Resources Recycling
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• v.20 no.3
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• pp.62-67
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• 2011

This study has been investigated for characteristic of removal on the effect of changes in soil moisture, microwave power, and temperature through the decomposition of oil-contaminated soil under high temperature conditions with high temperature heating elements and microwave. A lubricating oil having long carbocyclic(C18-C50) commonly known as a non-resolvable material was treated and the efficient for removal of TPH(Total Petroleum Hydrocarbon) obtained 85.2% at 6 kW and $700^{\circ}C$ and thus the contaminant was found to be removed to 1788 mg/kg for 40 minutes. In case of contaminated soil by light oil and gasoline, the amount of removal was achieved with 567 mg/kg at 6 kW, 500 for 20 minutes that corresponds to reach 98.4% of treatment efficient. In addition, non-resolvable materials contaminated by oil reached TPH concentration on 2,000 mg/kg of worrisome level of soil contamination in the 3-zone at 6kW, $700^{\circ}C$, and 30 minutes, and processing cost at this time was about 8,173 won per ton.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.31 no.5
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• pp.440-453
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• 2005

Objectives : To develop a bioactive membrane for guided bone regeneration (GBR), the biocompatibility and bone regenerating capacity of the cellulose membrane obtained from the Ascidians squirt skin were evaluated. Materials and methods : After processing the pure cellulose membrane from the squirt skin, the morphological study, amino acid analysis and the immunoreactivity of the cellulose membrane were tested. Total eighteen male Spraque-Dawley rats (12 weeks, weighing 250 to 300g) were divided into two control (n=8) and another two experimental groups (n=10). In the first experimental group (n=5), the cellulose membrane was applicated to the 8.0 mm sized calvarial bone defect and the same sized defect was left without cellulose membrane in the first control group (n=4). In the another experimental group (n=5), the cellulose membrane was applicated to the same sized calvarial bone defect after femoral bone graft and the same sized defect with bone graft was left without cellulose membrane in the another control group (n=4). Each group was sacrificed after 6 weeks, the histological study with H&E and Masson trichrome stain was done, and immunohistochemical stainings of angiogenin and VEGF were also carried out. Results : The squirt skin cellulose showed the bio-inductive effect on the bone and mesenchymal tissues in the periosteum of rat calvarial bone. This phenomenon was found only in the inner surface of the cellulose membrane after 6 weeks contrast to the outer surface. Bone defect covered with the bioactive cellulose membrane showed significantly greater bone formation compared with control groups. Mesenchymal cells beneath the inner surface of the bioactive cellulose membrane were positive to the angiogenin and VEGF antibodies. Conclusion : We suppose that there still remains extremely little amount of peptide fragment derived from the basement membrane matrix proteins of squirt skin, which is a kind of anchoring protein composed of glycocalyx. This composition could prevent the adverse immunological hypersensitivity and also induce bioactive properties of cellulose membrane. These properties induced the effective angiogenesis with rapid osteogenesis beneath the inner surface of cellulose membrane, and so the possibilities of clinical application in dental field as a GBR material will be able to be suggested.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.13 no.1
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• pp.15-22
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• 1980

It has been well known that histamine is presumably the causative material of an outbreak of allergy-like food Poisoning from eating of the meat of dark-fleshed fishes. The present paper was conducted to elucidate the changes in histamine content in the muscle of the dark-fleshed fishes, such as, common mackerel, Scomber japonicus, gizzard-shad, Konosirus punctatus and small sardine, Sardinops melanosticta, under different condition of storage and process. In general, the formation of histamine was markedly different depending upon the kind of fish and the storage temperature. The highest value of the amount of histamine was observed in common mackerel showing thirty folds of gizzard-shad, and gizzard-shad showed the lowest value. Referring to temperature, the formation of histamine at $10^{\circ}C$ was observed more rapid than that of at $25^{\circ}C$. According to the each products, the higher value observed in the sample of sun dried, and those of salted and hot-air dried were similar and boiled-dried of small sardine Was observed the lower value.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.1
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• pp.15-22
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• 1995

To study ecological properties of Vibrio cholerae non-Ol and Vibrio mimicus which have been described as new food poisoning bacteria recently, the influence of factors such as temperature, salinity, pH and chemical oxygen demand (COD) on detection rate and density of these bacteria were evaluated. Fifty four seawater samples and 49 bottom deposit samples from estuary of Kum river from March 26th, 1993 to February 22nd, 1994 were used for this study. The detection rate of V cholerae non-O1 were $16.7\%$ for seawater and $10.2\%$ for bottom deposit, respertively. The total detertion rate of V. cholerae non-O1 $(11.7\%)$ was a little higher than V mimicus $(10.7\%)$. Both V choierae non-O1 and V. mimicus were mainly detected in estuary water of which showed temperature $24^{\circ}C$ above and salinity $10\%o$ below. These bacteria were also detected in bottom deposit on January when the water temperature was $3.5^{\circ}C$. From these results, we supposed that temperature, salinity and organic material were important factors to growth of V. cholerae non-O1 and V. mimicus. V cholerae non-O1 might be grown better than V. mimicus under the fluctuating aquatic environmental condition such as salinity.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.2
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• pp.107-117
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• 1975

Alaska pollack caught in the Northern Pacific Ocean and frozen aboard vessel are skipped to the plant and processed into frozen fillets. In the present paper quality changes during thwaing, refreezing and storage at $-20^{\circ}C$ are discussed. Natural, running-water, vacuum and steam thawing were employed as thawing methods. And contact plate, air blast, immersion in dry ice-alcohol solution freezing and storage at $-5^{\circ}C$ were applied to refreeze the thawed fillets. As quality factors content of drip released, salt-extractable protein, VBN, DNA in the drip and pH were determined. In addition, bacteriological tests were also carried out along with the whole process. In thawing of round material, the vacuum thawing was more effective than any other method, resulting in drip, salt-extractable protein $(N\%)$, VBN and DNA as $4.4\%,\;1.82\%,\;16.21mg\%$ and $13.70\;{\mu}g/ml$, respectively. Storage at $-5^{\circ}C$ as refreezing method yielded lower in drip and DNA content but similar to or slightly higher in both salt-extractable protein and VBN, which might postulate that the quality of the frozen fillet depends not largely on the secondary freezing but on the conditions of thawing and primary freezing. It seemed that most of the bacterial flora in thawed fillet came from skin and viscera of fish, worker's hands, utensils and other processing facilities, since sanitary indicative bacteria were not detected in the frozen flesh of round Alaska pollack. Bacterial quality of fillet varied with thawing methods, vacuum thawing appeared more sanitative compared with other methods as natural, running-water, and steam thawing. Bacterial colonies isolated from the thawed fillet were composed of $73.8\%$ Gram negative rod shape, $4.9\%$ Gram positive rod shape, $18.0\%$ cocci, and $3.3\%$ yeast. Decreasing rate of coliform group of the fillet during the storage at $-20^{\circ}C$ for 30 days was more than $70\%$ and that of plate count was less than of coliform group.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2002.05a
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• pp.23-28
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• 2002

This paper describes the machining characteristics of the $MoSi_2$ based composites by electric discharge drilling with various tubular electrodes, besides, Hardness characteristics and microstructures of $Nb/MoSi_2$ laminate composites were evaluated from the variation of fabricating conditions such as preparation temperature, applied pressure and pressure holding time. $MoSi_2$ -based composites has been developed in new materials for jet engine of supersonic-speed airplanes and gas turbine for high- temperature generator. Achieving this objective may require new hard materials with high strength and high temperature-resistance. However, With the exception of grinding, traditional machining methods are not applicable to these new materials. Electric discharge machining (EDM) is a thermal process that utilizes a spark discharge to melt a conductive material, the tool electrode being almost non-unloaded, because there is no direct contact between the tool electrode and the workpiece. By combining a nonconducting ceramics with more conducting ceramic it was possible to raise the electrical conductivity. From experimental results, it was found that the lamination from Nb sheet and $MoSi_2$ powder was an excellent strategy to improve hardness characteristics of monolithic $MoSi_2$. However, interfacial reaction products like (Nb, Mo)$SiO_2$ and $Nb_2Si_3$ formed at the interface of $Nb/MoSi_2$ and increased with fabricating temperature. $MoSi_2$ composites which a hole drilling was not possible by the conventional machining process, enhanced the capacity of ED-drilling by adding $NbSi_2$ relative to that of SiC or $ZrO_2$ reinforcements.