• Journal of Microbiology and Biotechnology
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• v.23 no.10
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• pp.1445-1453
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• 2013

The scale-up criterion of constant oxygen mass transfer coefficient ($k_La$) was applied for the production of itaconic acid (IA) in a 50 L pilot-scale fermentor by the fungal cells of Aspergillus terreus. Various operating conditions were examined to collect as many $k_La$ data as possible by adjusting the stirring speed and aeration rate in both 5 L and 50 L fermentor systems. In the fermentations performed with the 5 L fermentor, the highest IA production was obtained under the operating conditions of 200 rpm and 1.5 vvm. Accordingly, we intended to find out parallel agitation and aeration rates in the 50 L fermentor system, under which the $k_La$ value measured was almost identical to that ($0.02sec^{-1}$) of the 5 L system. The conditions of 180 rpm and 0.5 vvm in the 50 L system turned out to be optimal for providing almost the same volumetric amount of dissolved oxygen (DO) into the fermentor, without causing shear damage to the producing cells due to excessive agitation. Practically identical fermentation physiologies were observed in both fermentations performed under those respective operating conditions, as demonstrated by nearly the same values of volumetric ($Q_p$) and specific ($q_p$) IA production rates, IA production yield ($Y_{p/s}$), and specific growth rate (${\mu}$). Specifically, the negligible difference of the specific growth rate (${\mu}$) between the two cultures (i.e., $0.029h^{-1}$ vs. $0.031h^{-1}$) was notable, considering the fact that ${\mu}$ normally has a significant influence on $q_p$ in the biosynthesis of secondary metabolites such as itaconic acid.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.17 no.1
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• pp.48-56
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• 2018

Due to the growing concerns of energy resource depletion and environmental destruction, the mass production of microalgae has been studied. The scale-up of a photobioreactor (PBR) is required for the mass production of biomass. In this paper, the geometric parameters and oxygen transfer rate (OTR) are considered, to scale up a flat panel photobioreactor (FP PBR). The PBR is designed using the goal-driven optimization (GDO) method to accomplish the scale-up. The local sensitivity of each output parameter with respect to the input parameter is analyzed through the design of experiment (DOE), and the design candidates are evaluated with the screening sampling method. The volumetric mass transfer coefficient is measured by the dynamic method.

• Journal of the Korean Society for Precision Engineering
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• v.11 no.6
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• pp.109-116
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• 1994

Quickness and precision are the two most important requirements for an industrial scale used in production lines. In this paper, a new approach, "Dynamic-Mass measurement control System of Acceleration and Displacement(DMS-AD) sensing", is presented to improve some of drowbacks in conventional scales. The system, consisted of acceleration and displace- ment sensors, spring scale and microcomputer, is based on full utilization of dynamic mass measurement of acceleration and displacement via microcomputer-assisted real time monitoring. The rsulting system, when combined with appropriate dynamic mass estimation algorithm software, has shown its effectiveness in terms of two desirable characteristics required. required.

• Korean Journal of Plant Resources
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• v.19 no.3
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• pp.432-435
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• 2006

A rapid and mass propagation method for multiple shoots and plant regeneration using bulb scales of Muscari comosum var. plumosum were developed. In vitro different parts of bulb scale as explants were cultured on 11 kinds of MS (1962) media supplemented with various plant growth regulators to induce shoot and callus. A combination of 2.0 mg/L 6-BA and 2.0 mg/L IBA on MS medium was the most favorable and induced the highest production (80%) of shoot formation after 30 days. We also found that the middle part of bulb scale was the best for mass propagation of Muscari comosum var. plumosum of which production could reach 64.4%.

• Transactions of the Korean Society of Automotive Engineers
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• v.7 no.7
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• pp.1-14
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• 1999

In the present study, the flame factor which primarily influence the simulation accuracy of the combustion process in a gasoline engine was modeled as a nonlinear function of turbulent intensity to laminar flame speed ratio. Multi-length-scale production rate model for turbulent kinetic energy equation was introduced to consider the different length scales of the swirling and tumbling motions in cylinder on the production rte of turbulent kinetic energy. By7 introducing the multi-length-scale production rate model for the turbulent kinetic energy equation, the predictions of turbulent burning velocity , cylinder pressure, mass burning rate and engine performance of a gasoline engine can much be improved.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.201-207
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• 2000

The strategies of commercial development have been focused on the economy of scale for a process. The design of media has been recognized as a key in assuring mass production of entomopathogenic nematodes. Media optimization was conducted with insect host, proteins, lipids, and symbiotic bacteria mass. G. mellonella (insect host) produced about 290,000 infective juveniles per one. Complex media produced about 250,000 infective juveniles / ml in liquid culture within 8 days (one generation).

• Applied Science and Convergence Technology
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• v.27 no.5
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• pp.79-85
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• 2018

Many studies have been conducted on large-scale graphene synthesis by chemical vapor deposition. Furthermore, numerous researchers have attempted to develop processes that can continuously fabricate uniform and high-quality graphene. To compete with other types of carbon materials (carbon black, carbon fiber, carbon nanotubes, and so on), various factors, such as price, mass manufacturing capability, and quality, are crucial. Thus, in this study, we examine various large-scale graphene production methods focusing on cost competitiveness and productivity improvements for applications in various fields.

• The Journal of the Acoustical Society of Korea
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• v.19 no.5
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• pp.71-78
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• 2000

Quickness and precision are the two most important requirements for an industrial scale used in production lines. In this paper, a new approach, "Mass-estimation algorithm by vibration-response measurement of dynamic balance", is presented to improve some of drawbacks in conventional scales. The system, consisted of velocity and displacement sensors, spring scale, analog-digital converter and microcomputer, is based on full utilization of dynamic mass measurement of velocity and displacement via microcomputer-assisted real time monitoring. The resulting system, when combined with appropriate mass estimation algorithm software, has shown its effectiveness in terms of two desirable characteristics required.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.6
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• pp.528-534
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• 2005

Hairy root cultures have demonstrated great promise in terms of their biosynthetic capability toward the production of secondary metabolites, but continue to constitute a major challenge with regard to large-scale cultures. In order to assess the possibility of conducting mass production of biomass, and the extraction of useful metabolites from Panax ginseng. P. ginseng hairy roots, transformed by Rhizobium rhizogenes KCTC 2744, were used in bioreactors of different types and sizes. The most effective mass production of hairy roots was achieved in several differently Sized air bubble bioreactors compared to all other bioreactor types. Hairy root growth was enhanced by aeration, and the production increased with increasing aeration rate in a 1 L bioreactor culture. It was determined that the hairy root growth rate could be substantially enhanced by increases in the aeration rate upto 0.5vvm, but at aeration rates above 0.5vvm, only slight promotions in growth rates were observed. In 20 L air bubble bioreactors, with a variety of inoculum sizes, the hairy roots exhibited the most robust growth rates with an inoculum size of 0.1% (w/v), within the range 0.1 to 0.7% (w/v). The specific growth rates of the hairy root decreased with increases in the inoculum size.

• Proceedings of the Plant Resources Society of Korea Conference
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• 1996.09a
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• pp.31-33
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• 1996