• Title/Summary/Keyword: mass propagation system

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ACTUAL STATE AND PRACTICAL USE OF THE FACTORY-STYLE PLANT PRODUCTION SYSTEM USING TISSUE CULTURE

  • Holdgate, D.P.;Zandvoort, E.A.
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 1996.05a
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    • pp.41-62
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    • 1996
  • Since 1966 tissue culture has been used as a tool for the production of disease indexed stocks from selected plants and their rapid (clonal) mass propagation through the procedure now referred to as micropropagation. The major advantages have been the rapid introduction of new plant cultivars, created within conventional and mutation breeding programmes, as healthy stock for beneficial distribution and the expansion of the world wide horticultural industry. (omitted)

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Mass-production of Eleutherococcus seoulensis Seedlings Through Somatic Embryogenesis (체세포배 형성을 통한 서울오갈피(Eleutherococcus seoulensis) 묘목의 대량생산)

  • Lee, Su-Gwang;Kang, Ho-Duck
    • Journal of Korean Society of Forest Science
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    • v.98 no.6
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    • pp.719-725
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    • 2009
  • This study was conducted to establish the optimal condition for acclimatization from somatic embryos of Eleutherococcus seoulensis. Torpedo-shaped embryos of Eleutherococcus seoulensis were cultured on 1/3 MS and WPM media supplemented with $GA_3$ (3.0, 5.0 mg/L) for 4 weeks. Plentlets were transferred to 1/2 SH solid medium with 1.0 mg/L $GA_3$ and 0.2% activated charcoal for shoot and root elongation and them elongated plantlets further developed on 1/2 SH medium for 4 weeks. Developed plantlets further elongated into well-shaped leaf and root system on 1/3 SH medium under ventilation condition for 4 weeks. Plantlets grew normally on 1/3 SH basal medium, were acclimated on various soil. Survival frequency of plantlets was influenced by soil type(peatmoss+perlite, perlite, soil on Nam mountain). The highest survival rate to soil was more than 70% when plantlets were 1/3 SH medium under ventilation condition in Nam mountain soil. These results indicate that the systematic procedure of plant production in Eleutherococcus seoulensis could be practically applicable for mass propagation.

Mass Propagation of Dicentra spectabilis L. Lemaire Through In vitro Suspension Culture (현탁배양을 통한 금낭화(Dicentra spectabilis L. Lemaire)의 대량증식)

  • Lee, Kang-Seop;Sim, Ock-Kyeong;Shin, Jeong-Sun;Choi, Yong-Eui;Kim, Ee-Yup
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.121-126
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    • 2004
  • Bleeding heart (Dicentra spectabilis L. Lemaire) is one of the most valuable wild flower in Korea. This work was conducted for the mass production of somatic embryos through suspension culture and more effective plant regeneration system in Dicentra spectabilis. High-frequency embryogenic callus proliferation was achieved in SH liquid medium supplemented with 1 mg/L 2,4-D. Half-strength SH medium was suitable concentration for somatic embryo induction and germination. About 5,000 embryos were produced per 250$m\ell$ flask after 4 weeks of culture. Germination rate of somatic embryos was decreased when GA$_3$ was added in medium. The plantlets showed a 58% survival rate when transferred to pots after 1 month of culture. The results indicate that micropropagation procedure via somatic embryogenesis can be applied for an efficient mass propagation of Dicentra spectabilis.

LIFE-SPAN SIMULATION AND DESIGN APPROACH FOR REINFORCED CONCRETE STRUCTURES

  • An, Xuehui;Maekawa, Koichi;Ishida, Tetsuya
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2007.04a
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    • pp.3-17
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    • 2007
  • This paper provides an introduction to life-span simulation and numerical approach to support the performance design processes of reinforced concrete structures. An integrated computational system is proposed for life-span simulation of reinforced concrete. Conservation of moisture, carbon dioxide, oxygen, chloride, calcium and momentum is solved with hydration, carbonation, corrosion, ion dissolution. damage evolution and their thermodynamic/mechanical equilibrium. Coupled analysis of mass transport and damage mechanics associated with steel corrosion is presented for structural performance assessment of reinforced concrete. Multi-scale modeling of micro-pore formation and transport phenomena of moisture and ions are mutually linked for predicting the corrosion of reinforcement and volumetric changes. The interaction of crack propagation with corroded gel migration can also be simulated. Two finite element codes. multi-chemo physical simulation code (DuCOM) and nonlinear dynamic code of structural reinforced concrete (COM3) were combined together to form the integrated simulation system. This computational system was verified by the laboratory scale and large scale experiments of damaged reinforced concrete members under static loads, and has been applied to safety and serviceability assessment of existing structures. Based on the damage details predicted by the nonlinear finite element analytical system, the life-span-cost of RC structures including the original construction costs and the repairing costs for possible damage during the service life can be evaluated for design purpose.

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Multiple Shoot Induction and Bulb Mass Proliferation System by in Vitro Immature Spathe Culture of Elephant Garlic (Allium ampeloprasum L.) (코끼리마늘(Allium ampeloprasum L.)의 기내 미숙총포 배양을 통한 다신초유도와 종구대량증식 시스템)

  • Kwon, Young Hee;Jeong, Jae Hyun;Lee, Jae Sun;Jeon, Jong Ok;Park, Young Uk;Min, Ji Hyun;Chang, Who Bong;Lee, Sang Young;Youn, Cheol Ku;Kim, Ki Hyun
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.355-362
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    • 2018
  • This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 cm length was cultured right after harvesting. In MS medium supplemented with 2 mg/L kinetin and 0.5 mg/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 cm, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 mg/L kinetin and 0.5 mg/L NAA, the addition of 30 mg/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture.

A Plastic Analysis of Structures under the Impact Loading (충격하중(衝擊荷重)을 받는 구조물(構造物)의 소성(塑性)모델에 따른 거동분석(擧動分析))

  • Ahn, Byoung Ki;Lee, Sang Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.12 no.2
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    • pp.21-33
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    • 1992
  • Under the intense-impulsive loading, structures are subjected to the wide range of pressures at an instantaneous time. The constitutive laws capable to describe the material behavior under the extreme pressure as well as the low pressure are necessary for the analysis of the structural behavior under the intense -impulsive loadings. In this study, two plastic models, the pressure independent Von-Mises model and the pressure dependent Drucker-Prager model, are employed for the wave propagation analysis. Governing equations of this study are conservation equations of momentum and mass in Lagrangian coordinate system which is fixed to the material. Due to the shock-front which violates the continuity assumptions inherent in the differential equations numerical artificial viscosity is used to spread the shock front over several computational zones. These equations are solved by Finite Difference Method with discretized time and space coordinates. The associate normality flow rule as a plastic theory is implemented to find the plastic strains.

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Centrifuge Simulation of Wave Propagation and Isolation Method (환경진동의 지반내 전파특성과 차단에 관한 원심모형실험)

  • Lee, Kang-Il;Kusakabe, O.;Kim, Chan-Kee;Kim, Tae-Hoon;Sul, Jin-Sung
    • Proceedings of the Korean Geotechical Society Conference
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    • 2004.03b
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    • pp.738-745
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    • 2004
  • There are a number of ways to reduce the ground vibrations, one of which is by installing underground walls. Model tests for ground vibration have been conducted in recent years, but limited attention has been paid to underground wall which can reduce high vibrations. Up to date, only barriers have been actually installed in dry sand because of many unknown factors subsisting on the behavior of the ground. The characteristics of vibration sources, ground conditions and wall barriers have not been well understood yet, therefore centrifugal modeling was adopted to examine all these characteristics. This paper describes a ball dropping system, which can generate a pulse wave propagation through soil mass, and the test results show the effectiveness of underground wall barrier in reducing mechanical vibration.

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The design of neural network adaptive control system (신경회로망 적응제어시스템의 설계)

  • 김용택;김용호;이홍기;전홍태
    • 제어로봇시스템학회:학술대회논문집
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    • 1993.10a
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    • pp.150-155
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    • 1993
  • The neural network MRAC system is presented. The purpose of this paper is applied to a plant that is to be controlled in a strongly nonlinear environment. The proposed system has a learning and adaptive ability in the varying environment by using the back-propagation learning algorithm based on Lyapunov stability theory. N.N. regulator is a part of overall system and is guaranteed to be stable in initial stage. Nonlinear terms of the varying mass, colilori, centifugal, and gravity are compensated for by feedforward N.N. regulator. And the feedback controller (adaptive mechanism) works to eliminate errors of position, velocity which the feedforward controller cannot compensate for. Finally, the proposed system will be demonstrated by simulation of a two d.o.f robot manipulator.

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Cyberattack Tracing System Operational Architecture (사이버공격 추적시스템 운용아키텍처)

  • Ahn, Jae-hong
    • Journal of the Korea Institute of Military Science and Technology
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    • v.26 no.2
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    • pp.179-187
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    • 2023
  • APT cyber attacks have been a problem for over a past decade, but still remain a challenge today as attackers use more sophisticated techniques and the number of objects to be protected increases. 'Cyberattack Tracing System' allows analysts to find undetected attack codes that penetrated and hid in enterprises, and to investigate their lateral movement propagation activities. The enterprise is characterized by multiple networks and mass hosts (PCs/servers). This paper presents a data processing procedure that collects event data, generates a temporally and spatially extended provenance graph and cyberattack tracing paths. In each data process procedure phases, system design considerations are suggested. With reflecting the data processing procedure and the characteristics of enterprise environment, an operational architecture for CyberAttack Tracing System is presented. The operational architecture will be lead to the detailed design of the system.