• 한국전기전자재료학회논문지
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• 제22권5호
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• pp.448-454
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• 2009

Hydrogen was produced from water plasma excited in high frequency (HF) inductively coupled tubular reactor. Mass spectrometry was used to monitor gas phase species at various process conditions, Water dissociation rate depend on the process parameters such as ICP power, $H_{2}O$ flow-rate and process pressure, Water dissociation percent in ICP reactor decrease with increase of chamber pressure, while increase with increase of ICP power and $H_{2}O$ flow rate. The effect of $CH_4$ gas addition to a water plasma on the hydrogen production has been studied in a HF ICP tubular reactor. The main roles of $CH_4$ additive gas in $H_{2}O$ plasma are to react with 0 radical for forming $CO_x$ and CHO and resulting additional $H_2$ production. Furthermore, $CH_4$ additives in $H_{2}O$ plasma is to suppress reverse-reaction by scavenging 0 radical. But, process optimization is needed because $CH_4$ addition has some negative effects such as cost increase and $CO_x$ emission.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1430-1437
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• 2021

Cronobacter sakazakii is an opportunistic pathogenic bacterium found in powdered infant formula and is fatal to neonates. Antibiotic resistance has emerged owing to overuse of antibiotics. Therefore, demand for high-yield bacteriophages as an alternative to antibiotics has increased. Accordingly, we developed a modified mass-production method for bacteriophages by introducing a two-stage self-cycling (TSSC) process, which yielded high-concentration bacteriophage solutions by replenishing the nutritional medium at the beginning of each process, without additional challenge. pH of the culture medium was monitored in real-time during C. sakazakii growth and bacteriophage CS01 propagation, and the changes in various parameters were assessed. The pH of the culture medium dropped to 5.8 when the host bacteria reached the early log phase (OD₅₄₀ = 0.3). After challenge, it decreased to 4.65 and then recovered to 4.94; therefore, we set the optimum pH to challenge the phage at 5.8 and that to harvest the phage at 4.94. We then compared phage production during the TSSC process in jar-type bioreactors and the batch culture process in shaker flasks. In the same volume of LB medium, the concentration of the phage titer solution obtained with the TSSC process was 24 times higher than that obtained with the batch culture process. Moreover, we stably obtained high concentrations of bacteriophage solutions for three cycles with the TSSC process. Overall, this modified TSSC process could simplify large-scale production of bacteriophage CS01 and reduce the unit cost of phage titer solution. These results could contribute to curing infants infected with antibiotic-resistant C. sakazakii.

• 한국가금학회지
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• 제18권2호
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• pp.67-84
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• 1991

사료의 단백질 수준이 산란계의 산란기별 생산성에 미치는 영향을 구명하기 위하여 methionine수준을 0.32%, lysine수준을 0.64%로 동일하게 하였을 때 단백질 수준을 달리하는 4개 처리(CP 12%. 13%, 14% 및 15%)에 22주령의 갈색산란계 384수를 공시하여 1989년 1월 28일부터 1990년 3월 23일까지 60주간에 걸친 사양시험과 대사시험 결과를 요약하면 다음과 같다. 1. 산란율은 산란초기에는 단백질 15%, 산란중기에는 단백질 14%, 산란말기에는 단백질 13%수준에서 가장 높았으며, 산란초기와 산란중기 및 산란전기간에서는 처리간에 통계적인 유의성이 인정되었다 (P＜0.05). 2. 난중은 단백질 15%와 14%수준간에는 유의적인 차이가 없었으나 그 이하의 수준에서는 단백질 수준이 낮아질수록 점차 감소하였으며, 산란초기(P＜0.01), 산란중기 및 산란말기에 통계적인 유의성이 인정되었다(P＜0.05). 3. 1일 1수당 산란량은 단백질 수준이 높아질수록 점차 증가하는 경향이었으며 산란초기와 산란중기에서는 고도의 유의성이 인정되었으나(P＜0.01), 단백질 15%수준에 비하여 산란초기에는 14%, 산란중기에는 13% 및 산란말기에는 12%수준까지 큰 차이가 없었다. 4. 사료섭취량은 단백질 15%와 14%수준간에는 차이가 없었으나 13% 이하에서는 점차 감소하는 경향이었으며, 산란초기와 산란중기에서는 고도의 유의성이 인정되었다(P＜0.01). 5. 사료요구율은 단백질 수준이 높아질수록 점차 개선되는 경향이었으며, 산란초기 (P＜0.01)와 산란중기 및 산란전기간에서는 통계적인 유의성이 인정되었다(P＜0.05). 6. 성계생존율은 단백질 수준이 높아질수록 점차 향상되는 경향이었으나 처리간에 통계적인 유의성은 인정되지 않았다. 7. 시험사료의 건물, 조단백질, 조지방 및 에너지의 이용율은 처리간에 차이가 없었으나, 탄수화물 이용율은 단백질 수준이 높아질수록 유의적으로 증가하는 경향이었다 (P＜0.05). 8. 도체율과 복강지방 축적율은 처리간에 일정한 경향이나 유의차가 없었다. 9 난각질은 사료의 단백질 수준간에 유의적인 차이가 없었다. 10. 계란의 일반성분과 아미노산 조성은 사료중의 단백질 수준에 영향을 받지 않았다. 11. 산란 kg당 사료비는 단백질 13%수준에서 가장 절감되었으나 처리간 통계적인 유의성은 인정되지 않았다.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.952-959
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• 2007

His-His-Leu (HHL), a tripeptide derived from a Korean soybean paste, is an angiotensin-I-converting enzyme (ACE) inhibitor. We report here a method of producing this tripeptide efficiently by expressing tandem multimers of the codons encoding the peptide in E. coli and purifying the HHL after hydrolysis of the peptide multimers. The HHL gene, tandemly multimerized to a 40-mer, was ligated with ubiquitin as a fusion gene (UH40). UH40 was inserted into vector pET29b; the UH40 fusion protein was then produced in E. coli BL21. The recombinant UH40 protein was purified by cation-exchange chromatography with a yield of 17.3mg/l and analyzed by matrixassisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry and protein N-terminal sequencing. Leucine aminopeptidase was used to cleave a 405-Da HHL monomer from the UH40 fusion protein and the peptide was purified using reverse-phase high-performance liquid chromatography (HPLC) on a C18 HPLC column, with a final yield of 6.2mg/l. The resulting peptide was confirmed to be HHL with the aid of MALDI-TOF mass spectrometry, glutamine-TOF mass spectrometry, N-terminal sequencing, and measurement of ACE inhibiting activity. These results suggest that our production method is useful for obtaining a large quantity of recombinant HHL for functional antihypertensive peptide studies.

• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1393-1400
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• 2009

A bacteriocin-producing strain PI80 was isolated from the gut of Penaeus indicus (Indian white shrimp) and identified as Streptococcus phocae PI80. The bacteriocin was purified from a culture supernatant to homogeneity as confirmed by Tricine SDS-PAGE. Reverse-phase HPLC analysis revealed a single active fraction eluted at 12.94 min, and MALDI-TOF mass spectrometry analysis showed the molecular mass to be 9.244 kDa. This molecular mass does not correspond to previously described streptococcal bacteriocins. The purified bacteriocin was named phocaecin PI80 from its producer strain, as this is the first report of bacteriocin production by Streptococcus phocae. The bacteriocin exhibited a broad spectrum of activity and inhibited important pathogens: Listeria monocytogenes, Vibrio parahaemolyticus, and V. fischeri. The antibacterial substance was also sensitive to proteolytic enzymes: trypsin, protease, pepsin, and chymotrypsin, yet insensitive to catalase, peroxidase, and diastase, confirming that the inhibition was due to a proteinaceous molecule (i.e., the bacteriocin), and not due to hydrogen peroxide or diacetyl. Phocaecin PI80 moderately tolerated heat treatment (up to $70^{\circ}C$ for 10 min) and resisted certain solvents (acetone, ethanol, and butanol). A massive leakage of $K^+$ ions from E. coli $DH5\alpha$, L. monocytogenes, and V. parahaemolyticus was induced by phocaecin PI80, as measured by Inductively Coupled Plasma Optical Emission Spectrometry (ICPOES). Therefore, the results of this study show that phocaecin PI80 may be a useful tool for inhibiting L. monocytogenes in seafood products that do not usually undergo adequate heat treatment, whereas the cells of Streptococcus phocae PI80 could be used to control vibriosis in shrimp farming.

• 한국광학회지
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• 제8권3호
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• pp.189-193
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• 1997

광 pick-up용 비구면 대물 렌즈는 컴퓨터와 멀티미디어 기기 등의 발달로 수요가 급증하고 있으며 사출성형에 의하여 대량 생산되고 있다. 광 pick-up용 비구면 대물 렌즈의 생산성 향상을 위해서는 생산공정중의 전수검사를 요구하며 이는 측정환경에 강인한 측정기를 필요로 하고 있다. 본 연구에서는 생산공정중에 광 pick-up용 비구면 대물 렌즈를 측정할 수 있는 개선된 층밀리기 간섭계를 제안하고자 한다. 본 층밀리기 간섭계는 3개의 직각 프리즘과 1개의 광분할 코팅된 프리즘으로 구성되며 index matching oil에 의하여 상대이동이 가능하도록 조합되어 있다. 간섭무늬를 정확히 분석하기 위한 위상천이와 층밀림량의 조절은 프리즘 간의 상대 이동에 의하여 가능하며 이를 위한 구동 기구부를 제작하였다. 또한, 본 연구에서는 반복연산에 의한 일반 알고리즘을 도입하여 위상천이 시에 발생하는 index matching oil의 유막 두께변화에 의한 기준위상 오차를 보상하였다. 광 pick-up용 비구면 대물 렌즈의 수차량을 정량적으로 산출하기 위하여 Zernike 다항식 맞춤을 수행하였다. 본 간섭계는 측정환경에 매우 강인하며 방진과 밀폐가 없는 열악한 측정환경하에서 반복측정을 수행하였을 때 .lambda./100 이하의 반복능을 얻었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권4호
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• pp.301-305
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• 2001

The co-expression of the arg U gene in a double-vector expression system of recombi-nant Escherichia coli BL22(DE3)[pET-IEN2a+pAC-argU] significantly enhanced the production level of reconminant human interferon -$\alpha$2a(rhIFN-$\alpha$2a) in high cell density cultures, compared to a recombinant E. coli culture containing only the single expression vector, pET-IEN2a. The dry cell mass concentration increased to almost 100 g/L, and more than 4 g/L of rhIFN-$\alpha$2a was accumu-lated in the culture broth. Evidently, the synthesis of rhIFN-$\alpha$2a was strongly dependent on the pre-induction growtih rate and more efficient at a higher specific growth rate. The additional sup-ply of tRN $A^{Arg(AGG/AGA)}$ enhanced the expression level of the rhIFN-$\alpha$2a gene in the early stage of the post-induction phase, yet thereafter the specific production rate of rhIFN-$\alpha$2a rapidly de-creased due to severe segregational instability of plasmid vector pET-IEN2a. It would appear that the plasmid instability with only occurred to pET-IEN2a in the double vector system, was re-lated to the effect of translational stress due to the over expression of rhIFN-$\alpha$2a.

• Journal of Microbiology and Biotechnology
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• 제25권6호
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• pp.803-813
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• 2015

The growth of Irpex lacteus F17 and manganese peroxidase (MnP) production in a selfdesigned tray bioreactor, operating in solid-state conditions at a laboratory scale, were studied. The bioreactor was divided into three layers by three perforated trays. Agroindustrial residues were used both as the carrier of bound mycelia and as a nutrient medium for the growth of I. lacteus F17. The maximum biomass production in the bioreactor was detected at 60 h of fermentation, which was consistent with the CO₂ releasing rate by the fungus. During the stationary phase of fungal growth, the maximum MnP activity was observed, reaching 950 U/l at 84 h. Scanning electron microscopy images clearly showed the growth situation of mycelia on the support matrix. Furthermore, the MnP produced by I. lacteus F17 in the bioreactor was isolated and purified, and the internal peptide sequences were also identified with mass spectrometry. The optimal activity of the enzyme was detected at pH 7 and 25℃, with a long half-life time of 9 days. In addition, the MnP exhibited significant stability within a broad pH range of 4-7 and at temperature up to 55℃. Besides this, the MnP showed the ability to decolorize the polymeric model dye Poly R-478 in vitro.

• The Plant Pathology Journal
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• 제40권4호
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• pp.346-357
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• 2024

This study was carried out to screen the antifungal activity against Colletotrichum acutatum, Colletotrichum dematium, and Colletotrichum coccodes. Bacterial isolate GP-P8 from pepper soil was found to be effective against the tested pathogens with an average inhibition rate of 70.7% in in vitro dual culture assays. 16S rRNA gene sequencing analysis result showed that the effective bacterial isolate as Bacillus siamensis. Biochemical characterization of GP-P8 was also performed. According to the results, protease and cellulose, siderophore production, phosphate solubilization, starch hydrolysis, and indole-3-acetic acid production were shown by the GP-P8. Using specific primers, genes involved in the production of antibiotics, such as iturin, fengycin, difficidin, bacilysin, bacillibactin, surfactin, macrolactin, and bacillaene were also detected in B. siamensis GP-P8. Identification and analysis of volatile organic compounds through solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) revealed that acetoin and 2,3-butanediol were produced by isolate GP-P8. In vivo tests showed that GP-P8 significantly reduced the anthracnose disease caused by C. acutatum, and enhanced the growth of pepper plant. Reverse transcription polymerase chain reaction analysis of pepper fruits revealed that GP-P8 treated pepper plants showed increased expression of immune genes such as CaPR1, CaPR4, CaNPR1, CaMAPK4, CaJA2, and CaERF53. These results strongly suggest that GP-P8 could be a promising biocontrol agent against pepper anthracnose disease and possibly a pepper plant growth-promoting agent.

• KSBB Journal
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• 제22권3호
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• pp.162-167
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• 2007

암모니아 가스는 환경오염 중 가장 피해가 큰 물질로 가축 뿐만 아니라 사람에게도 많은 피해를 준다. 본 연구의 목적은 Bacillus subtilis IB101을 이용하여 암모니아 가스의 제거효율이 좋은 조건과 최적으로 배양할 수 있는 생산배지의 최적화에 대하여 알아보는 것이다. 암모니아 제거 효과가 우수한 균주인 B. subtilis IB101의 암모니아 가스 제거 효능이 좋은 성장단계를 알아보았으며, 기본배지의 구성성분 중 $(NH_4)_2SO_4$의 농도에 변화를 주어 각각 다른 $(NH_4)_2SO_4$ 농도의 배지에서 배양된 cell이 암모니아 가스 제거에 미치는 영향을 알아보았다. 그리고 온도 및 pH에 따른 암모니아 가스 제거 효능의 최적조건을 알아보았다. B. subtilis IB101의 대량 배양을 위한 배지 조성을 얻기 위하여 Placket-Burman design과 one factor at a time method를 이용하여 기본배지의 구성성분의 영향에 대하여 살펴보고 최적의 배지조성을 알아보았다. Exponential phase 단계인 B. subtilis IB101을 접종한 것이 stationary phase 단계인 B. subtilis IB101을 접종한 것보다 암모니아 가스 제거 효율이 약 20% 정도 우수하였다. 30$^{\circ}C$, pH 4의 배양조건에서 암모니아 가스 제거 효율이 가장 높았다. 기본 배지 구성성분 중 $(NH_4)_2SO_4$의 농도의 변화에 따른 암모니아 가스 제거 효율에는 영향이 없었다. 최적화된 배지의 구성 성분은 yeast extract 10 g/l, soluble starch 2.5 g/1, $MgSO_4$ 6 g/l, $CaCl_2$ 1.55 g/1, $(NH_4)_2SO_4$ 5 g/1, $KH_2PO_4$ 0.75 g/l, 대두분 8 g/l이었다.