• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.717-724
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• 2016

Methane (CH₄) is the most abundant component in natural gas. To reduce its harmful environmental effect as a greenhouse gas, CH₄ can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. In this study, several methanotrophs were examined for their ability to produce methanol from CH₄; including Methylocella silvestris, Methylocystis bryophila, Methyloferula stellata, and Methylomonas methanica. Among these methanotrophs, M. bryophila exhibited the highest methanol production. The optimum process parameters aided in significant enhancement of methanol production up to 4.63 mM. Maximum methanol production was observed at pH 6.8, 30℃, 175 rpm, 100 mM phosphate buffer, 50 mM MgCl₂ as a methanol dehydrogenase inhibitor, 50% CH₄ concentration, 24 h of incubation, and 9 mg of dry cell mass ml^-1 inoculum load, respectively. Optimization of the process parameters, screening of methanol dehydrogenase inhibitors, and supplementation with formate resulted in significant improvements in methanol production using M. bryophila. This report suggests, for the first time, the potential of using M. bryophila for industrial methanol production from CH₄.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.19 no.2
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• pp.139-144
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• 2006

A fabrication condition of the cathode electrode was optimized in a lithium secondary battery. The $LiNi_{1/3}Mn_{1/3}Co_{1/3}O_2$ powders were used as a cathode material. The $LiNi_{1/3}Mn_{1/3}Co_{1/3}O_2$/Li cells were prepared with a certain formulation and their cycleability and rate-capability were evaluated. Optimum electrode composition simulated from the evaluated value was 86.3: 5.6: 8.1 in mass $\%$ of active material: binder: conducting material. Discharge capacity decreased markedly as the press ratio exceeded $30\%$ during preparation of the electrode. Discharge performance at a high current rate deteriorated abruptly as the electrode thickness was over $120{\mu}m$.

• Macromolecular Research
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• v.14 no.2
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• pp.209-213
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• 2006

The ring-opening polymerization of D,L-lactide initiated by tin(II) 2-ethylhexanoate $(Sn(Oct)_2)$ on the surface-initiated magnetite $(Fe_{3}O_4)$ nanoparticles was performed at $130^{\circ}C$. The effects of the polymer molar mass and concentration on the amount of surface polymer were investigated. The number average molecular weights, $M_n$, obtained by both NMR and GPC methods fit well within the accuracy of the applied methods and ranged from 1,100 to $4,040g\;mol^{-1}$. A surface functionalization density of up to 625 initiation sites per particle was obtained. The composition of various core-shell particles was determined by TGA, with results indicating magnetite $(Fe_{3}O_4)$ contents, ${\mu}m$, between 17 and 59 wt%. Under the influence of a magnetic field, the heating generated by superparamagnetic core-shell particles suspended in toluene presented guidelines for an optimization of magnetic particle systems with respect to an application for hyperthermia.

• Journal of Korean Society on Water Environment
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• v.33 no.2
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• pp.187-196
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• 2017

To ensure hygienic safety of drinking water in a water storage tank, the concentrations of residual chlorine should be above a certain regulation level. In this study, we conducted model simulations to investigate the effects of temperature on residual chlorine in water storage tank conditions typically used in Seoul. For this, values of model parameters (decomposition rate constant, sorption coefficient, and evaporation mass transfer coefficient) were experimentally determined from laboratory experiments. The model simulations under continuous flow conditions showed that the residual chlorine concentrations were satisfied the water quality standard level (0.1 mg/L) at all the temperature conditions ($5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$). Meanwhile, when the tanks had a no flow condition (i.e., no tap-water influent due to a sudden shut-down), the concentrations became lower than the regulatory level after certain periods. The findings from this modeling works simulating Seoul's water storage tanks suggested disappearance rate of residual chlorine could be reduced through the tanks design optimization with maintenance of low water temperature, minimization of air flow and volume, suppression of dispersion and the use of wall materials with low sorption ability.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.1
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• pp.24-30
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• 2016

1-Deoxynojirimycin (DNJ) have been extensively investigated for their α-glucosidase inhibitor on postprandial hyperglycemia, and applied in nutraceuticals and medicine for preventing or delaying progression of type 2 diabetes. However, the amount of DNJ in mulberry leaves is low (about 0.1%), therefore, more effective extraction method is needed. This study was performed to develop microbial DNJ for biological methods of DNJ as an alternative to the chemical methods. In this study, we obtained evidence for Bacillus subtilis that produce DNJ in large quantities by high performance liquid chromatography. Inhibition of α-glucosidase activity was determined to DNJ production or non-production. Investigation of the effect of mulberry leaves powder concentration (1~5%), using the DNJ high-production bacteria, provided evidence for microbial mass production of DNJ. When the 4% mulberry leaf powder for 9 days was used, the α-glucosidase inhibitory activity was over the 85%. Also, the results presented in this study confirm DNJ yield's increasement in microbes using the various of nutrients and provide insight of ways to improve DNJ yields in microorganisms.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.22 no.3
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• pp.251-258
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• 2009

At the stage conceptual design, combat vehicle is classified into three general categories of fire power, mobility and physical properties of system. The present research is restricted to fire power and its optimization. At the stage of conceptual designing of system, it is appropriate to consider major variables affecting fire power - including the weight of bullet, which exerts a direct influence on destroying effect, maximum range which takes long range firing in consideration. To estimate the maximum firing range, a simple interior ballistic and an exterior ballistic model were built by using the lumped parameter method, Le Duc method and point mass trajectory model. Design of experiment and regression analysis was used to derive simulations of fire power. Finally, response surface models were built and design variables were analyzed.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2002.07a
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• pp.67-73
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• 2002

The development of high performance HTS wire is a key factor for various electrical applications of coils and cables. The purpose of this paper is to review and consider the main manufacturing technologies of HTS wire and its current status. A lot of efforts have been focused on the optimization of PIT parameters for Bi-2223/Ag wire. According to this, long Bi-2223 wires having Ic of 130 A were recently produced and their mass production has been underway in US. The current status performance of Bi-2223 wire is supposed to be used in power transmission cable because of its lower self-field property. Y-123 second generation conductor is extensively being developed throughout the world and many fabrication processes are competed with each other. 30 m-long Y-123 wire with Ic of 0.8 MA/$\textrm{cm}^2$ was recently fabricated using IBAD and PLD techniques in Japan. This result offers promise of scalable processing of practical multi-layer coated conductor.

• 유체기계공업학회:학술대회논문집
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• 2000.12a
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• pp.163-168
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• 2000

A method to search the design parameters for optimum stage matching has been used based on a 1-D mathematical model of a compressor, which uses the data obtained from the preliminary test to identify the design parameters. This methodology was applied with a two-stage axial compressor, which was originally designed for a helicopter gas turbine engine. After Identifying design parameters using preliminary test data, an optimization process has been employed to achieve the best matching between the stages (i.e., maximum efficiency of the compressor at its operation modes within a given range of the rotor speed under given restrictions for required stall margins and mass flow). 3-D flow calculations have been performed to confirm the usefulness of the corrections based on 1-D mathematical model. Calculational results agree well with the experimental data in view of the performance characteristics. Some promising results were produced through the methodology proposed in this paper in conjunction with flow calculations.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.12
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• pp.1997-2003
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• 2003

Recently, there have been many researches and developments in optical disc drive by high density of track and high access speed. Therefore, the optical pick-up should guarantee highly accurate dynamic characteristics for the wide bandwidth in order to cope with this trend. These demands for optical pick-up actuator can be solved by improvements of lens-holder through the following methods. The first way is the analysis of the sensitivity matrix of design variables for vibration modes after appropriate design parameters are selected like shapes and local dimensions of a lens-holder. The second way is the optimization of design variables by calculating sequential linear programming after the problem of extending bandwidth are converted to problem of minimizing adequate objective function. In the result, modified FE model is obtained through several iterations by finite difference scheme(FDS). While results of the first way show better convergence of the target frequency, the second result shows better reduction of mass increase.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.6
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• pp.687-693
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• 2011

Spring-mass models have been widely accepted to explain the basic dynamics of human gait. Researchers found that the leg stiffness increased with gait speed to increase energy efficiency. However, the difference of leg stiffness change with gait speed between the young and the elderly has not been verified yet. In this study, we calculated the lower limb stiffness of the elderly using walking model with an axial spring. Vertical stiffness was defined as the ratio of the vertical force change to the vertical displacement change. Seven young and eight elderly subjects participated to the test. The subjects walked on a 12 meter long, 1 meter wide walkway at four different gait speeds, ranging from their self-selected speed to maximum speed randomly. Kinetic and kinematic data were collected using three force plates and motion capture cameras, respectively. The vertical stiffness of the two groups increased as a function of walking speed. Maximum walking speed of the elderly was slower than that of the young, yet the walking speed correlated well with the optimal stiffness that maximizes propulsion energy in both groups. The results may imply that human may use apparent limb stiffness to optimize energy based on spring-like leg mechanics.