• Nuclear Medicine and Molecular Imaging
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• v.41 no.4
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• pp.299-308
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• 2007

Purpose: The aim of this study was to develop a bioinformatics software and to test it in serum samples of papillary thyroid cancer using mass spectrometry (SELDI-TOF-MS). Materials and Methods: Development of 'Protein analysis' software performing decision tree analysis was done by customizing C4.5. Sixty-one serum samples from 27 papillary thyroid cancer, 17 autoimmune thyroiditis, 17 controls were applied to 2 types of protein chips, CM10 (weak cation exchange) and IMAC3 (metal binding - Cu). Mass spectrometry was performed to reveal the protein expression profiles. Decision trees were generated using 'Protein analysis' software, and automatically detected biomarker candidates. Validation analysis was performed for CM10 chip by random sampling. Results: Decision tree software, which can perform training and validation from profiling data, was developed. For CM10 and IMAC3 chips, 23 of 113 and 8 of 41 protein peaks were significantly different among 3 groups (p<0.05), respectively. Decision tree correctly classified 3 groups with an error rate of 3.3% for CM10 and 2.0% for IMAC3, and 4 and 7 biomarker candidates were detected respectively. In 2 group comparisons, all cancer samples were correctly discriminated from non-cancer samples (error rate = 0%) for CM10 by single node and for IMAC3 by multiple nodes. Validation results from 5 test sets revealed SELDI-TOF-MS and decision tree correctly differentiated cancers from non-cancers (54/55, 98%), while predictability was moderate in 3 group classification (36/55, 65%). Conclusion: Our in-house software was able to successfully build decision trees and detect biomarker candidates, therefore it could be useful for biomarker discovery and clinical follow up of papillary thyroid cancer.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.4
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• pp.348-353
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• 2008

Oxygen isotope has not been used actively in water mass studies because of difficulties on the analysis though it has advantages as a water mass tracer. The most popular method to analysis the oxygen isotope ratio in water samples is equilibration method: isotopic equilibrium of water with $CO_2$ at constant temperature. The precision of oxygen isotope analysis using commercial automatic $H_2O/CO_2$ equilibrator is ${\pm}0.1%o$. This value is not sufficient for studies in open ocean. The object of this study is to improve the analytical precision enough to apply open ocean studies by modification of the instrument. When sample gas is transferred by the pressure difference, the fractionation which is preferential transportation of light isotope can be occurred since the long transportation path between the equilibrator and mass spectrometer. And the The biggest source of error during the analysis is long distance and large volume of the pathway of sample gas between. Therefore, liquid nitrogen trap and high vacuum system are introduced to the system. The precisions of 14 time analysis of same seawater sample are ${\pm}0.081%o$ and ${\pm}0.021%o$ by built-in system and by modified system in this study, respectively.

• The Journal of Engineering Geology
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• v.7 no.2
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• pp.151-159
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• 1997

Since healed microcracks in quartz grain of grantic rocks within the same mass have identical preferred orientations, the oreintations of granitic cores may be determined if the distinctive feature of healed microcracks can be used.In this study, the possibility of determining orientations of granitic cores using healed microcrack orientations were examined using samples from the borehole drilled to 200 m in depth at the Hongcheon. Eight sections whose core recoveries are 100% were selected. Two to six samples were collected in each section and orientations of healed microcracks in each sample were measured. Healed microcracks in samples from each section show almost identical orientations. The error range for sections with only one preferred orientations is within $\pm$5$^{\circ}$, indicating that correct orientations of core can be determined. However, orientations of cores in sections which have 2 or more healed microcrack orientations should be determined using orientations as well as distribution of peaks of orientations. The error range for this case is lager than former one and is within $\pm$15$^{\circ}$. The orientations of joint which is very impontant factor for designing tunnel and slope stability can be determined using healed microcrack orientation in cores.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.999-1004
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• 2005

An algorithm for a hybrid controller consists of a sliding mode control part and a fuzzy logic part which ar purposely for nonlinear systems. The sliding mode part of the solution is based on "eigenvalue/vector"-type controller is used as the backstepping approach for tracking errors. The fuzzy logic part is a Mamdani fuzzy model. This is designed by applying sliding mode control (SMC) method to the dynamic model. The main objective is to keep the update dynamics in a stable region by used SMC. After that the plant behavior is presented to train procedure of adaptive neuro-fuzzy inference systems (ANFIS). ANFIS architecture is determined and the relevant formulation for the approach is given. Using the error (e) and rate of error (de), occur due to the difference between the desired output value (yd) and the actual output value (y) of the system. A dynamic adaptation law is proposed and proved the particularly chosen form of the adaptation strategy. Subsequently VSC creates a sliding mode in the plant behavior while the parameters of the controller are also in a sliding mode (stable trainer). This study considers the ANFIS structure with first order Sugeno model containing nine rules. Bell shaped membership functions with product inference rule are used at the fuzzification level. Finally the Mamdani fuzzy logic which is depends on adaptive neuro-fuzzy inference systems structure designed. At the transferable stage from ANFIS to Mamdani fuzzy model is adjusted for the membership function of the input value (e, de) and the actual output value (y) of the system could be changed to trapezoidal and triangular functions through tuning the parameters of the membership functions and rules base. These help adjust the contributions of both fuzzy control and variable structure control to the entire control value. The application example, control of a mass-damper system is considered. The simulation has been done using MATLAB. Three cases of the controller will be considered: for backstepping sliding-mode controller, for hybrid controller, and for adaptive backstepping sliding-mode controller. A numerical example is simulated to verify the performances of the proposed control strategy, and the simulation results show that the controller designed is more effective than the adaptive backstepping sliding mode controller.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.33 no.4
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• pp.271-277
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• 2020

There are increasing cases of monitoring the structural response of structures using multiple sensors. However, owing to cost and management problems, limited sensors are installed in the structure. Thus, few structural responses are collected, which hinders analyzing the behavior of the structure. Therefore, a technique to predict responses at a location where sensors are not installed to a reliable level using limited sensors is necessary. In this study, a numerical study is conducted to predict the seismic response of low-rise buildings using limited information. It is assumed that the available response information is only the acceleration responses of the first and top floors. Using both information, the first natural frequency of the structure can be obtained. The acceleration information on the first floor is used as the ground motion information. To minimize the error on the acceleration history response of the top floor and the first natural frequency error of the target structure, the method for predicting the mass and stiffness information of a structure using the genetic algorithm is presented. However, the constraints are not considered. To determine the range of design variables that mean the search space, the parameter prediction method based on artificial neural networks is proposed. To verify the proposed method, a five-story structure is used as an example.

• Journal of Astronomy and Space Sciences
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• v.13 no.2
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• pp.173-180
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• 1996

This paper briefly describes the KITSAT-1 and KITSAT-2 spacecrafts and presents the functions, calibration procedures and in-orbit results of the KITSAT-2 analog sun sensors have been flown as an experimental payload for the future mission. We have two constraints in their design: small size and very low power consumption due to the tight mass and power budget of the spacecraft. Two one-dimensional analog sun sensors are mounted on the top facet of the KITSAT-2 spaceraft. Each has $\pm$60 degrees of view angle and they cover 210 degree field of view in total as the 30 degree view angles are overlapped. Only the relative sun angle around the Z-axis (yaw-axis) and the spin rate of the spacecraft can be achieved as the one dimensional sun sensors are used and they are aligned with the Z-axis. The calibration formulae are obtained using the fifth order line fitting algorithm for each sun sensor on the ground and they are applied to the obtained in-orbit data. ASS-1 with silicon solar cells has maximum error of 1.5 degree and ASS-2 with silicon photocells manufactured at KAIST has maximum error of 0.5 degree except near 0 degree of sun ray incident anagle where random reflection of incident sun ray is maximum in orbit. The results are presented in chapter 4. The performance of each sun sensor and the possible mounting errors are stated in chapter 5.

• Journal of Animal Science and Technology
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• v.50 no.2
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• pp.177-184
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• 2008

Acid-labile subunit(ALS) is a 85-kDa glycosylated plasma protein which forms a 150-kDa ternary complex with 7.5-kDa insulin-like growth factor(IGF) and 40~45-kDa IGF-binding protein-3. In a previous study, the present authors prepared a porcine ALS(pALS) expression construct by inserting a pALS coding sequence into a plasmid vector following synthesis of the sequence by reverse transcription-polymerase chain reaction(RT-PCR). The expression construct, however, was subsequently found to have a mis-sense mutation at two bases of the pALS coding sequence which is presumed to have occurred through a PCR error. In the present study, the correct coding sequence was synthesized by the site-directed mutagenesis and inserted into the pET-28a(+) plasmid expression vector containing the His-tag sequence flanking the last codon of the insert DNA. After induction of the expression construct in E. coli BL21(DE3) cells, the resulting presumptive recombinant peptide was purified by the Ni-affinity chromatography. Upon SDS- PAGE, the affinity-purified peptide was resolved as a single band at a 66-kDa position which is consistent with the expected molecular mass of the presumptive recombinant pALS. Collectively, results indicate that a recombinant pALS peptide was successfully expressed and purified in the present study.

• Journal of the Korean Chemical Society
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• v.27 no.4
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• pp.279-286
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• 1983

An apparatus was made for the electrodeposition of alpha emitting actinide nuclides, $^{207}Bi$ and $^{210}Po$. The electrodeposition was made on a polished stainless steel plate cathode. The anode was made of platinum wire and to stir the solution. With the ammonium chloride as electrolyte initial pH = 4, chloride concentration = 0.6M and solution volume = 15ml, a current of 1.5 ampere(current density = 0.59A/$cm^2$) was flowed for 100 minutes for the quantitative recovery of electrodeposition and on average recovery of 98.3% was obtained within ${\pm}$0.7% uncertainty. Alpha spectrometry of the electrodeposited sample showed alpha peaks from $^{210}Po, ^{234}U$ and $^{239}Pu$ having energy resolution (FWHM) of 18.3, 21.8 and 36.0 keV respectively. The electrodeposition and alpha spectrometry for a natural uranium sample of domestic origin gave $^{238}U : ^{234}U = 1 : 6.1{\times}10^{-5}$ and for a neutron-irradiated uranium sample did $^{238}U : ^{239}Pu : ^{241}Am = 100 : 0.0263 : 5.20{times}10^{-5}$. The result of $^{238}U$ determination in the irradiated sample by electrodeposition-alpha spectrometry was in accord within ${\pm}1.6%$ of relative error with the results of solid fluorimetry and mass spectrometry. For $^{239}Pu$ the result of electrodeposition-alpha spectrometry was in accord within ${\pm}$4.0% of relative error with the results of anion exchange separation and the thenoyltrifluoroacetone(TTA) extraction both followed by alpha spectrometries.

• Tuberculosis and Respiratory Diseases
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• v.84 no.1
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• pp.46-54
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• 2021

Background: The aim of this study was to investigate inhaler device handling in elderly patients. Inhaler devices with respect to misuse and error correction were also compared. Methods: Inhaler use technique was assessed using standardized checklists at the first visit and 3-month follow-up visit after retraining. The primary outcome was difference in the acceptable use ratio among inhaler devices. Secondary outcomes included differences in error correction, the most common step of misuse, and factors affecting the accuracy of inhaler use. Results: A total of 251 patients (mean age, 76.4 years) were included. The handling of 320 devices was assessed in the study. All patients had been trained before. However, only 24.7% of them used inhalers correctly. Proportions of acceptable use for Evohaler, Respimat, Turbuhaler, Ellipta, and Breezhaler/Handihaler were 38.7%, 50.0%, 61.4%, 60.8%, and 43.2%, respectively (p=0.026). At the second visit, the acceptable use ratio had increased. There were no significant differences among inhaler types (Evohaler, 63.9%; Respimat, 86.1%; Turbuhaler, 74.3%; Ellipta, 64.6%; and Breezhaler/Handihaler, 65.3% [p=0.129]). In multivariate analysis, body mass index, Turbuhaler, and Ellipta showed positive correlations with acceptable use of inhalers, whereas Chronic Obstructive Pulmonary Disease Assessment Test score showed a negative correlation. Conclusion: Although new inhalers have been developed, the accuracy of inhaler use remains low. Elderly patients showed more errors when using pressurized metered-dose inhalers than using dry powder inhalers and soft-mist inhalers. However, there were no significant differences in misuse among inhaler devices after individual training. Results of this study suggests that repeat training is more important than inhaler type.

• Journal of The Korean Association For Science Education
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• v.27 no.5
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• pp.404-411
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• 2007

The purpose of this study was to examine the types of explanation about different experimental data on students' preconceptions on physical experiment. 45 students were selected from P University by examining their pre-test. The subjects had a scientific conception about a simple pendulum's period of movement, and expected that there was no change in the movement even when the mass of water increased in the can. The students were presented data about pendulum movement inside a can. They recorded this in a blank table and drew a graph about the movement of the pendulum. The responses of students were tested by presenting written anomalous experimental data. Students' recognition, trust about the anomalous data, cognitive conflict, belief change about their preconceptions were examined with written tests also. To measure students' cognitive conflict levels, CCLT (Cognitive Conflict Levels Test) developed in a previous study was used. Some students responded based on preconceptions and regarded the data as manufactured data or experimental error. The students who recognized the anomaly in the situation showed higher cognitive conflict levels than those who didn't recognize the anomaly in the situation. The students who changed their beliefs about preconceptions recognized the data as anomalous situations and showed high cognitive conflict levels than those who didn't abandon their preconceptions. The students who have unchanged beliefs about their preconceptions regarded the data as experimental error.