• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.704-709
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• 1996

This experiment was carried out to establish the system of mass propagation through tissue culture using sucker and stem in Ramie. The sterilization for tissue culture of Ramie was the better treatment of 2% NaClO for 20 minute into ultrasonic cleaner than the others, and so rate of contamination was 3.3%, and it was able to produce 96% healthy plant. The effect of growth regulator was superior to mixed treatment of 0.02mg/$\ell$ NAA, 1.5mg/$\ell$ BA, 0.lmgmg/$\ell$ GA$_3$, which it was not formed callus and but produced 96% healthy plant. The effect of propagation was higher in culturing of the stem node than the sucker in cultural part, local variety than improved ones. The effect of acclimatization was superior to pretreatment of 30 minute after soaking in 100ppm NAA, transplanting on bed soil which mixed to ratio of vermiculite : soil : sand =1 : 2 : 1, the transplanted plants were grown all normal.

• Magazine of the Korean Society of Agricultural Engineers
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• v.38 no.5
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• pp.106-115
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• 1996

This study was carried out to provide fundamental data for reuse of resources and prevention of environmental pollution Actual states of environmental pollution by biological production facilities were investigated. Various pollution loads and quantity of wastes from greenhouse and animal housings estimated. In greenhouse contents of investigation were wastes of plastic cover, noise of heater and fan, air pollutant of heater, and wastes of soilless culture system, etc. In animal housings, those were actual state of livestock waste treatment and reuse, pollutant mass unit discharge, noise pollution, and malodorant, etc. The main pollutants discharged from greenhouses were wastes of plastic cover, rockwool, and waste fluid of nutrient solution. Developments of waste disposal methods for plastic cover and rockwool are required. And recycle technique of nutrient solution for soilless culture should be developed and propagated. With the buildup of legal control, pollutant mass discharged from livestock wastes are, on the decrease. The other side, reuse of livestock excreta increased. Most animal housings are located near the dwelling house. Malodorant and noise from animal housings have bad effects on the rural living conditions. So developments of integrated complex for small scale livestock farms are required.

• The Research Journal of the Costume Culture
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• v.16 no.4
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• pp.634-645
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• 2008

The purpose of this study was to develop cruise look design inspired by the ocean life motif using by computer CAD system. Domestic and international brands were selected for this study researching cruise line collection. A variety of literature and online site for concept of cruise look and sustainable design was studied. Adobe Illustrator CS2 and Adobe Photoshop CS2 program were used for cruise look design through analysis of 07/08 S/S trend. The diffusion of cruise look reflects a societal trend to be changed life pattern. The results of this study are as below. First, as this society has become modernized with advancement of technology, people have been increasingly interested in the pursuit of happiness and the concept of 'quality of life', thereby creating a new trend of consumption culture. As a result, fashion market place changed targeting sensible and self-enrichment customers. Second, as combining rapid technological innovation and mass production, industrial design caused environmental pollution and the problems are being worsen. Fashion design has same problems and led to eco-friendly design by the large. Third, as the change of style influenced by economic and social condition, cruise look re-defined and became more common. The change of various fields led to fashion market place should be changed. The cruise look design using computer program will give more benefits to many designers.

• KSBB Journal
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• v.4 no.2
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• pp.143-149
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• 1989

A cell sedimentaion system was designed and employed for the high culture of hydridoma. An upward divering cell settler allowed a good sedimentation of hybridoma but the accumulation of cell mass on the settler's wall side was a potential prolem. Although a cyinderical cell settler was useful to solve this problem, this device was employable only at low dilytion rate. A modified cell settler could support the high density culture of hybridoma at a concentration of $5{\times}10^6$ cell/ ml during 1 week, producting 380mg of monclonal antibody.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.37-37
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• 2021

Calystegia soldanella L. is a perennial herbaceous halophyte belonging to the convolvulaceae family, which mainly grows in coastal sand dunes in Korea. Shoots and rhizomes are edible, and roots called 'Hyoseon Chogeun' are known to have medicinal effects such as antipyretic, sterilization, and diuretic. In addition, physiological activities of antioxidant, anti-inflammatory, antiviral, antifungal and PTP-1B (protein tyrosine phosphate-1B) inhibition have been reported. In this study, in vitro induction cell lines of C. soldanella L. collected from the coastal sand dunes in Jeju island was redifferentiated into adventitious roots that can be used as medicinal resources. Also the biomass of mass-proliferated adventitious roots using a bioreactor were evaluated. Plants of C. soldanella L. were collected from the crevice of the seashore in the coastal area of Taeheung 2-ri, Namwon-eup, Seogwipo-si. Then, it was separated into leaves, stems, rhizomes, and roots, and surface sterilized with 70% ethyl alcohol and 2% NaOCl (sodium hypochlorite). After washing with sterilized water, each organ section was cultured in Hormone-free MS medium (Murashige & Skoog Medium). As a result, the induction response rates were evaluated at 85% and 55%, respectively, in terms of callus formation and shoot generation in the rhizome segment. In the case of the adventitious roots morphological characteristics induced by single-use treatment of auxin-based plant growth regulators IBA and NAA from redifferentiated shoots were compared. Most efficient adventitious root culture method as a rooting rate, number, length, and biomass proliferation in the bioreactor system was confirmed when treated by culturing in MS salts, Sucrose 30 g·L^-1, and IBA 1mg·L^-1 for 4 weeks. In this study, the medium composition and culture period were confirmed using a bioreactor system to mass-proliferate adventitious roots derived from C. soldanella L. in Jeju island. Also this adventitious root line developed a new medicinal material could increase value of the bio-industry ingredient through quantitative and qualitative screening of phyto-bioactive compounds.

• Korean Journal of Medicinal Crop Science
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• v.20 no.6
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• pp.461-465
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• 2012

This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of $NH_4NO_3$ and $KNO_3$ at the ratio of 1650 (mg/${\ell}$) : 1900 (mg/${\ell}$) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with $170mg/{\ell}$ $KH_2PO_4$. The addition of 1 and 3% sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1566-1573
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• 2014

Although most algae cultivation systems are operated in suspended culture, an attached growth system can offer several advantages over suspended systems. Algal cultivation becomes light-limited as the microalgal concentration increases in the suspended system; on the other hand, sunlight penetrates deeper and stronger in attached systems owing to the more transparent water. Such higher availability of sunlight makes it possible to operate a raceway pond deeper than usual, resulting in a higher areal productivity. The attached system achieved 2.8-times higher biomass productivity and total lipid productivity of $9.1g\;m^{-2}day^{-1}$ and $1.9g\;m^{-2}day^{-1}$, respectively, than the suspended system. Biomass productivity can be further increased by optimization of the culture conditions. Moreover, algal biomass harvesting and dewatering were made simpler and cheaper in attached systems, because mesh-type substrates with attached microalgae were easily removed from the culture and the remaining treated wastewater could be discharged directly. When the algal biomass was dewatered using natural sunlight, the palmitic acid (C16:0) content increased by 16% compared with the freeze-drying method. There was no great difference in other fatty acid composition. Therefore, the attached system for algal cultivation is a promising cultivation system for mass biodiesel production.

• The Research Journal of the Costume Culture
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• v.1 no.1
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• pp.33-68
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• 1993

The purpose of this paper is to survey the influence of the culture change on the dress and personal adornments, and to survey the change of factors from the August 15. 1945 to 1960, in connection with the social and cultural background, applying B. Malinowski′s "Theory of Culture Change" which means an acculturation is the change′s elements of the dress and its ornaments. It is remarkable that the dress and personal adornments of Korean women after August 15, 1945 has great change from the traditional dress of the thousands years. Through the Liveration on August 15, 1945, American military government and the establishment of the Korean government, American democracy has brought many changes on Korean society. Advancement of education, an society with developed economic life, and introduction of scientific skill have influenced on the women′s way of thinking and a style of behavior and the view of value. The acculturation, the supply of mass communication, introduction of new institution and the reorganization of the old institution, the change of the economic situation, the change of the educational system, the change of the point of value, the development of scientific skill have greatly influenced on the dress and personal adornments. Modern dress and personal adornments of Korean women has the following developing procedure. The Liberation from Japan on the 15th, of August, 1945, the stationing of American Soldiers during hte Koran War, returning of the oversea′s brethern, and the mass communication have the direct and indirect contact with western civilization, and bring the occasion of development of the modern dress of Korean women. The increase of mass communication, such as radio, TV, newspaper, magazines and the frequent fashion shows showed fine design and practical western style dress to the women. This was the real beginning of the western style dress in Korea. By the increase of employment and the improvement of the economic situation, the consumption of clothing and the adornments was increased, so that a wide variety of fashions was set, and numerous kind and form of dress came in. As the increase of the number of women students, their practical and free dress life demanded western style dress. After the Liberation from Japan, the open-door policy of sex, public morals and traditional ethics became lax, and in this disordered society, indecent expose of the body was common. By the different kind of technological development, the form, fabrics, colors and patterns in clothes were much influenced and the hair style, make up, shoes and adornments were much influenced too. As shown above, the change of the dress has the same connection with that of society and culture. This shows that the dress and personal adornments represent clearly the cultural phenomenon of the society and cultural change of the society.

• Journal of Bio-Environment Control
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• v.23 no.2
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• pp.144-147
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• 2014

It was intended to closely examine an effect that a change in the concentration of culture medium had on the potato(Solanum tuberosum L.) plantlet growth in the microponic system so as to mass-produce the virus-free plant of new variety 'Saebong' for potato processing. The adjusted concentration of potato culture medium was 0.2, 0.6, 1.0, 1.4, 1.8, and $14.0dS{\cdot}m^{-1}$. And potato seedling was cut into pieces of 1.5 cm in length, which included 2 growth points and leaves. And each was explanted in glass vial of 50 mL. And experiments were carried out twice for 18 days or 21days. Culture medium of 2ml was put in the container respectively. And 1 mL was added after 10 days. And in terms of cultivation environment, the experiment was carried out at the day length of 16 hours at the temperature of $23{\pm}1^{\circ}C$ under the white LED light of $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The concentration of culture medium in the experiment I was EC 0.2, 1.0, $14dS{\cdot}m^{-1}$ and was adjusted to 0.6, 1.0, 1.4, $1.8dS{\cdot}m^{-1}$ in the experiment II. The results showed that the survival rate of plantlet was 90% at $0.2dS^2m^{-1}$, 100% at $0.6dS^2m^{-1}$, 100% at $1.0dS^2m^{-1}$. 0% at $1.4dS{\cdot}m^{-1}$, 0% at $1.8dS{\cdot}m^{-1}$. and 0% at $14.0dS{\cdot}m^{-1}$ after 7 days. With regard to the explanted potato seedling, in case of the treatment where the electrical conductivity of culture medium was adjusted to $1.0dS{\cdot}m^{-1}$, root developed 2 days after transplantation. And the plantlet vigorously grew into strong plant that had 7 leaves, length of 5cm, and fresh weight of 0.5 g after 18 days. In case of the treatment where the concentration of culture medium was adjusted to $0.6dS{\cdot}m^{-1}$, the root plantlets developed 4 days after transplantation. And those grew into plant that had 7 leaves and fresh weight of 0.2 g after 21 days. Therefore, we found that it is effective to control potato culture medium by adjusting its electrical conductivity to $0.6{\sim}1.0dS{\cdot}m^{-1}$ for the mass production of virus-free potato seedling in the microponic system.

• Korean Institute of Interior Design Journal
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• v.19 no.6
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• pp.188-196
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• 2010

The Commercial space are changed gradually large scalized and complexed to introduce different culture exchanges and experience. However, the mass-complex commercial space are limited as of their complexed space composition or degree of space depth, which may become factors causing people to get tired easily. Thus, having a design strategy is important to create economical effectiveness in order to overcome these limitations and for the people to stay longer and do their consumption activities through more experiences and social interchanges. Recently, Jerde Partnership's who designed many complex commercial spaces made a commercial success by their unique design strategy to setup a new space and lead the interaction between space and people. The purpose of this study is based on Jerd's design concept to analyze Jerd Partnership's design strategy and space related structure to propose their space construction and design method for mass-complex commercial spaces. The results are as follows. (1)Jerde Partnership's causes the abundant space experience of the user through mass and space constitution to have a theme and a story. (2)They builds an excursion type line of flow system with an organic curve, and a non-daily experience by the change of the space scale and the application of various programs is enabled and guides the stay for the long time. (3)They builds the doorway of various courses and a circulation system through the open space and controls depth of the space. In addition, various events are performed in the center of such a circulation system, and this event makes a unique place.