• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.791-792
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• 2003

• Ocean and Polar Research
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• v.36 no.3
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• pp.247-254
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• 2014

In our continuing study on the antioxidant activity of Polysiphonia stricta, its crude extract was fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions according to solvent polarity. The solvent fractions were evaluated for their potential to inhibit lipid peroxidation and reactive oxygen species (ROS) production in HT 1080 cells. The n-BuOH fraction most strongly inhibited both lipid peroxidation and ROS production in HT 1080 cells. The n-BuOH fraction was further separated by repeated silica gel column chromatography and RP-HPLC to give methyl aconitates (2 and 3). The chemical structure of isolated compounds was determinated by NMR spectral analysis.

• Proceedings of KOSOMES biannual meeting
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• 2017.11a
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• pp.171-171
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• 2017

• Journal of the Korean Applied Science and Technology
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• v.33 no.2
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• pp.278-285
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• 2016

The discovery of various activities of natural plants has increased interest in halophytes. Suaeda australis and S. maritime are perennial halophytes that belong to the Chenopodiaceae family. Extracts of S. australis and S. maritime plants were investigated for concentration and time-dependent antibacterial and antioxidant activities using bacterial species and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, respectively, as well as total phenolic content. The S. australis extract ($500{\mu}g/mL$) showed activity against all the bacterial species including P. aeruginosa, P. mirabilis, A. baumannii, and VRE with 61.1, 42.3, 44.49, and 40.38%, respectively, inhibition and suppressed of these four species for 12 h. Overall, the S. australis extract showed marked antibacterial activities while, in contrast, the S. maritime extract had excellent antioxidant effects. However, the effects of the two extracts were much lower than that of quercetin. The present study identified antibacterial activities of S. australis, and it would be necessary to perform further phytochemical studies of S. australis.

• KSBB Journal
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• v.29 no.4
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• pp.271-277
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• 2014

In this study, crude extract of the seagrass Zostera japonica, and its solvent-partitioned fractions were evaluated for their antioxidant activity. The crude extract was successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. These include DPPH radical scavenging, hydroxyl radical scavenging in HT-1080 cells, peroxynitrite scavenging, and protective effect on DNA damage caused by hydroxyl radicals generated. In all assays, except for DPPH radical, 85% aq.MeOH and n-BuOH fraction showed the strong antioxidant activity. These results suggest that Z. japonica may be used as a potential source of natural antioxidants for the development of cosmetic product or functional food in the future.

• Journal of the Korean Chemical Society
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• v.59 no.6
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• pp.545-550
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• 2015

• Journal of Applied Biological Chemistry
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• v.50 no.2
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• pp.74-77
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• 2007

Crude extracts of 69 marine organisms (27 salt marsh plants and 42 seaweeds) were screened for the inhibitory activity against the protein tyrosine phosphatase 1B (PTP1) in vitro. The most active extracts were methanol extracts from Derbesia marina (80.6% in inhibitory activity) and Symphycladia latiscula (85.6%) at the concentration of $15{\mu}g/mL$. Methanol extracts of Codium adhaerens and Hisikia fuziformis were moderately inhibitory with 71.2 and 69.1% inhibition, respectively. It was peculiar that only the extracts from seaweeds show inhibitory activity where those from salt marsh plants do not show any significant effect.

• Ocean and Polar Research
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• v.33 no.3
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• pp.255-263
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• 2011

The aim of this investigation was to evaluate antioxidant activity of crude extracts from the halophyte Vitex rotundifolia, their solvent fractions, and isolated compounds (1-3). Antioxidant capacity was determined by measuring DPPH radical, and authentic $ONOO^-$ and $ONOO^-$ generated from 3- morpholinsydnonimine (SIN-1) in vitro as well as degree of occurrence of intracellular ROS, NO and GSH in mouse macrophage Raw 264.7 cells. From comparative analysis, MeOH extract, n-BuOH, and 85% aq. MeOH solvent fractions showed significant antioxidant effect in DPPH radical and $ONOO^-$ assay systems. Activity-guided purification of n-BuOH and 85% aq. MeOH fractions led to the isolation of flavonoids 1-3. Among them, compound 1 exhibited excellent antioxidant effect in all bioassay systems tested. On the other hand, compounds 2 and 3 revealed potent inhibitory effect against $ONOO^-$ generated from SIN-1, comparable with the positive control penicillamine.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.3
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• pp.212-216
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• 2004

Peroxynitrite formation in vivo is implicated in numerous human diseases and there is considerable interest in the use of antioxidants and natural products for their treatment. The three components (1-3) isolated from Sargassum thunbergii as well as the organic solvent-soluble fractions and the aqueous layer of S. thunbergii were evaluated for their potential to scavenge authentic ONOO$\^$-/ and ONOO$\^$-/ derived from 3-morpholinosydnonimine (SIN-1). The antioxidant activity of the individual fractions was in the order of 85% aqueous (aq.) MeOH＞n$\^$-/ BuOH＞n-hexane＞H$_2$O. The three known compounds, sargahydroquinoic acid (1), sargaquinoic acid (2) and sargachromenol (3) showed peroxynitrite-scavenging activities comparable to those of L-ascorbic acid and penicillamine. These results showed a possible antioxidant activity in major constituents of S. thunbergii.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.1
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• pp.71-78
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• 2015

In order to deduce an objective evaluation method of motion seasickness incidence (MSI) by ship motions during underway in irregular waves and to present the fundamental data of passenger comfort on the yacht and the passenger ship according to the result, the MSI of the trainees by the questionnaires was analysed and compared with the rate of variation of salivary ${\alpha}$-amylase activity (VSAA) on the training ship "A-ra ho" of Jeju national university. Relationship between rate of variation (x) by salivary ${\alpha}$-amylase activity and motion seasickness incidence (y) was described by the equation, MSI(%) = 0.6073 x + 12.189 including the correlation coefficient ($R^2=0.9853$). The result obtained through the rate of variation of salivary ${\alpha}$-amylase activity which was the quantitative evaluation method for ship motions causing seasickness was most affected by z-vertical acceleration and occurred within the frequency range 0.1 to 0.3Hz centered on 0.2Hz, and the simulation result based on this finding showed the motion seasickness rate at approximately 4% lower than the rate obtained through the survey.