Core samples, drilled in the middle region of Bavaria, were analyzed to study the characteristics of organic matter in the Upper Jurassic Solnhofen limestone of southern Germany. The core (48$^{\circ}$53'N, 1-1$^{\circ}$19'E) contains Upper Jurassic Solnhofen strata ranging from the upper part of the Geisental Formation throughout the Solnhofen Formation to the lower part of the Mornsheim Formation. In the core, the Upper Jurassic lithologies consist of platy limestone, bedded limestone and massive limestone often interbedded with some chert layers. Geochemical variations (Carbon, Nitrogen and Total Organic Carbon) and Rock-Eval pyrolysis parameters (S$_2$ peak and Hydrogen Index) indicate that the organic matter in the Upper Jurassic limestone is mostly of marine origin. Particularly, the relation-ship of Hydrogen Index and S$_2$ as a function of Total Organic Carbon suggests that the upper formation of the core (Mornsheim Formation) was more influenced by terrigenous influx than the Solnhofen and Geisental Formations.
Suspension culture is a useful tool for culturing embryonic stem (ES) cells in large-scale, but the stability of pluripotency and karyotype has to be maintained $in$$vitro$ for clinical application. Therefore, we investigated whether the chromosomal abnormality of ES cells was induced in suspension culture or not. The ES cells were cultured in suspension as a form of aggregate with or without mouse embryonic fibroblasts (MEFs), and 0 or 1,000 U/ml leukemia inhibitory factor (LIF) was treated to suspended ES cells. After culturing ES cells in suspension, their karyotype, DNA content, and properties of pluripotency and differentiation were evaluated. As a result, the formation of tetraploid ES cell population was significantly increased in suspension culture in which ES cells were co-cultured with both MEFs and LIF. Tetraploid ES cell population was also generated when ES cells were cultured alone in suspension regardless of the existence of LIF. On the other hand, the formation of tetraploid ES cell population was not detected in LIF-free condition, in which MEFs were included. The origin of tetraploid ES cell population was turned out to be E14 ES cells and not MEFs by microsatellite analysis and the basic properties of them were still maintained despite ploidy-conversion to tetraploidy. Furthermore, we identified the ploidy shift from tetraploidy to near-triploidy as tetraploid ES cells were differentiated spontaneously. From these results, we demonstrated that suspension culture system could induce ploidy-conversion generating tetraploid ES cell population. Moreover, optimization of suspension culture system may make possible mass-production of ES cells.
Journal of the Korean Society of Fisheries and Ocean Technology
/
v.43
no.4
/
pp.355-361
/
2007
A giant jellyfish (Nemopilema nomurai), which is presumed developing in the East China Sea, is recently one of major issues in the Northeast Asia region due to its fatal damage to the fishery. The biomass estimates have generally been conducted by trawl sampling and sighting survey methods. The biological research is also needed to clarify such environmental origin or diurnal migration patterns. While trawl sampling or sighting survey methods are effective to investigate its density estimates in its distributed community of near bottom or surface, they have a problem in investigation on the vertical distribution of jellyfishes. In this case, an echo sounding detection would have an advantage to survey it more extensively and effectively. This trial was conducted to observe the vertical distribution of giant jellyfish, where thermocline strongly formed, during mooring at each station of the East China Sea and southern coastal area using acoustical and optical methods. By the results, they were observed to exit and move at the water column under the thermocline using the optical camera and echo sounder system, and the information was analyzed to find out the acoustical sound scattering characteristics relatives to 120kHz frequency. These results can be utilized effectively to estimate the vertical distribution and biomass of Giant jellyfish with comparing results from trawl sampling and sighting survey methods, hereafter.
The coal-bearing metapelites from the Hoenam area are interbedded within the Ogcheon Supergroup, which are composed mainly of graphite, quartz, muscovite and associated with trace amount of biotite, chlorite, pyrite, rutile and barite. Although the formation was mined for coal, and the contents of V, U and Mo are a higher grade in coal formations than the host metapelites. The ratios of Al/Na and K/Na in coal formation are very heterogeneous and wide range from 10.28 to 90.91 and from 3.73 to 36.60, respectively. The V content increase with increasing Al and Ba contents, but the U and Mo are not related with other elements. Those are suggested that controlled of mineral compositions in coaly metapelites due to substitution, migration and reequilibrium of elements by regional metamorphism. These coal formation were deposited in basin of marine environments and the REE of these rocks are not influenced with metamorphism and hydrothermal alterations on the basis of Al content versus La, La against Ce, the ratios of La/Ce (0.23 to 0.73) and Th/U (0.03 to 16.6). These rocks also show much variation in $La_N/Yb_N$ (0.53 to 14.19), Th/Yb (0.51 to 6.00) and La/Th (0.15 to 18.92), and their origin is explained by derivation from a mixture of sedimentary and metasedimentary rocks. The wide range in trace and REE element characteristics as Co/Th (0.07 to 3.00), La/Sc (0.04 to 23.48), Sc/Th (0.06 to 7.57), V/Ni (2 to 3319), Cr/V (0.03 to 1.06) and Ni/Co (1.00 to 79.85) of these coaly metapelites argues for inefficient mixing of the various source lithologies during sedimentation.
The middle Cretaceous stratigraphec section of Deep Sea Drilling Project (DSDP) Site 530 in the Angola Basin is characterized by cyclic interbeds of organic-carbon-rich black shales and organic-carbon-poor red and green claystones, namely the black shale sequence. A number of samples from the black shale sequence were analyzed for the typesand distribution of insoluble sedimentary organic matter(kerogen) in order to give more information on the depositional conditions of the black shales in the Angola Basin. The dominant type of kerogen in the black shale sequence at Site 530 is amorphous organic matter mainly of marine planktonic algal origin. It probably consists of remains of some unfossiliqed dinoflagellates. The cyclic preservation of organic-carbon-rich black shales in the Angola Basin during the mid-Cretaceous could be explained by the low dissolved-oxygen concentration in the warm, saline deep and bottom waters combined with the sluggish circulation within the highly restricted basin, and the periodic high productivity in the surface waters.
A long core (RS15-LC48) was collected at a site in the continental rise between the Southern Ocean and the Ross Sea (Antarctica) during the 2015 Ross Sea Expedition. The mineralogical characteristics and the origin of clay minerals in marine sediments deposited during the Quaternary in the Ross Sea were determined by analyzing sedimentary facies, variations in grain size, sand fraction, mineralogy, clay mineral composition, illite crystallinity, and illite chemical index. Core sediments consisted mostly of sandy clay, silty clay, or ice rafted debris (IRD) and were divided into four sedimentary facies (units 1-4). The variations in grain size distribution and sand content with depth were very similar to the variations in magnetic susceptibility. Various minerals such as smectite, chlorite, illite, kaolinite, quartz, and plagioclase were detected throughout the core. The average clay mineral composition was dominated by illite (52.7 %) and smectite (27.7 %), with less abundant clay minerals of chlorite (11.0 %) and kaolinite (8.6 %). The IC and illite chemical index showed strong correlation trends with depth. The increase in illite and chlorite content during the glacial period, together with the IC and chemical index values, suggest that sediments were transported from the bedrocks of the Transantarctic Mountains. During the interglacial period, smectite may have been supplied by the surface current from Victoria Land, in the western Ross Sea. High values for IC and the illite chemical index also indicate relatively warm climate conditions during that period.
The Arctic Ocean is very sensitive to global warming and Arctic Ocean sediments provide a records of terrestrial climate change, analyzing their composition helps clarify global warming. The gravity core sediment ARA07C-St02B was collected at the East Siberian margin during an Arctic expedition in 2016 on the Korean ice-breaking vessel ARAON, and its provenance was estimated through sedimentological, mineralogical and geochemical analysis. The core sediment was divided into four units based on sediment color, sand content and ice-rafted debris content. Units 1 and 3 had higher sand and ice-rafted debris contents than units 2 and 4, and contained a brown layer, whereas units 2 and 4 were mainly composed of a gray layer. Correlation analysis using the adjacent core sediment ARA03B-27 suggested that the sediment units were deposited during marine isotope stage 1 to 4. The bulk mineral, clay mineral, and geochemical compositions of units including a brown layer differed from units including a gray layer. Bulk and clay mineral compositions indicated that coarse and fine sediments had a different origin. Coarse sediments might have been deposited mostly by the East Siberian Coastal Current from the Laptev Sea and the East Siberian Sea or by the Beaufort Gyre from the Chukchi Sea, whereas fine sediments might have been transpoted mostly by currents from the East Siberian Sea, the Chukchi Sea and the Beaufort Sea. Some of the coarse sediments in unit 1 and fine sediments in unit 3 might have been deposited by iceberg ice, sea ice or current from the Beaufort Sea and the Canada Archipelago. Investigating the geochemical composition of the potential origins will elucidate the origin and transportation of the study area's core sediments.
Kim Jinho;Park Soo-chul;Kang Moo-hee;Kim Kyong-O;Han Hyun-chul
Economic and Environmental Geology
/
v.38
no.6
s.175
/
pp.643-656
/
2005
The East Sea, a marginal sea or back-arc basin, consists of Japan Basin, Yamato Basin, and Ulleung Basin and is surrounded by the Pacific Plate and Philippine Sea Plate. Ulleung Basin locates in the southwestern part of the East Sea and shows the depth of 1,500 m in average and 2,500 m in maximum, connecting to the Japan Basin along 2,000 m contour. The slope of the seafloor is greater in the western side of the basin than in the southern and the eastern side. The crustal thickness of the Ulleung Basin from the OBS tends to get thicker toward the north and the west side and the sediment thickness of the Ulleung Basin is getting thicker toward the southeast side and reaches up to 12 km. The crustal type of the Ulleung Basin was variously suggested as like as a rifted continental crust, an extended continental crust, and an incipient oceanic trust. The origin of the crustal formation and the Ulleung Basin, however, is still controversial. Based on the bathymetry and gravtiy anomaly data for this study, the axis of the Ulleung Basin shows that the basin develops along the axis trending NW-SE direction and reveals a general symmetry of the bathymetry. And also the free-air gravity anomalies show a very similar pattern to the bathymetry of the basin. The sediment thickness is relatively thicker in the southeastern side of the basin than in the northwestern side. Although the crustal age of the Ulleung Basin is supposed to be younger than them of the Japan Basin and the Yamato Basin, the free-air gravity anomalies of the Ulleung Basin ranging -40 to 50 mGals are lower than the other basins, which suggests that the densities of crust and sediment of the Ulleng Basin are lower than the Japan Basin and the Yamato Basin.
Metal concentrations in creek water, sewer outlets and core sediments were analyzed to identify the potential origin of metal pollution and to evaluate the extent of metal pollution and potential toxicity of Lake Shihwa. Mean concentrations for dissolved metals in creek water and sewer outlets were 1.6~136 times higher than those in the surface seawater of Lake Shihwa. Metal concentrations in creek water from an industrial region were also higher than those from municipal and agricultural regions, indicating that the potential source of metal pollution in the study area might be mainly due to industrial activities. The vertical profiles of metals in core sediments showed an increasing trend toward the upper sediments. Extremely higher concentrations of metals were observed in the vicinity of Banweol industrial complex. The results of a geo-accumulation index indicated that Cu, Zn and Cd were highly polluted. By comparing the sediment quality guidelines such as TEL and PEL, six metals such as Cr, Ni, Cu, Zn, Cd and Pb levels in core sediments nearby industrial complex exceeded the PEL value. Mean PEL quotient (mPELQ) was used to integrate the estimate of potential toxicity for measured metals in the present study. Mean PELQs in core sediments from Lake Shihwa ranged from 0.2~2.3, indicating that benthic organisms nearby the industrial complex may have been adversely affected.
Journal of the Korean Society of Marine Environment & Safety
/
v.23
no.5
/
pp.513-523
/
2017
We evaluated the viability of phytoplankton along the salinity gradient in the flood and ebb tides of spring tide of February and the ebb tide of neap tide of March 2017 in the Seomjin River Estuary. Additional laboratory experiments were also conducted to determine the reason of the pH changes along the salinity gradient using the field natural sample in February. In field, saltwater was well mixed at downstream vertically and the salinity gradient was horizontally appeared toward upstream of freshwater zone. There were strong negative correlations between salinity and nutrient (nitrate + nitrite R=0.99, p<0.001, and silicate R=0.98, p<0.001), implying that those two nutrients of freshwater origin were gradually diluted with mixing the saltwater. On the other hands, relatively high phosphate concentration was kept in the stations of saltwater over 15 psu, indicating that it was caused by resuspended sediments of Gwangyang Bay and downstream by tidal water mixing.Among phytoplankton community structure in winter, Eucampia zodiacus have occupied to be c.a. 70 % in the most stations. Based on the field survey results for survivability of phytoplankton by phytoPAM instrument, there was positive correlations between salinity and chlorophyll a (R=0.82, p<0.001) and, salinity and active chlorophyll a (R=0.80, p<0.001), implying that the dominant marine diatom species may have significantly damaged in low salinity conditions of upstream. Also, maximum mortality rate of phytoplankton caused by low salinity shock was appered to be 75% in the upstream station. In particular, the pH in spring tides of February had tended to increase with high phytoplankton accmulated stations, suggesting that it was related with absorption of $CO_2$ by the photosynthesis of dominant diatom. In laboratory experiments, phytoplankton mass-mortality caused by low salinity shock was also occurred, which is confirmed with reducing the photosynthetic electron transport activity. Following the phytoplankton mass-mortality, bacteria abundance was significantly increased in 24 hours. As a result, the mass-proliferating bacteria can produce the $CO_2$ in the process of biodegradation of diatoms, which can lead to pH decrease. Therefore, marine phytoplankton species was greatly damaged in freshwater mixing area, depending on along the salinity gradient that was considered to be an important role in elevating and reducing of pH in Seomjin River Estuary.
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