• Animal cells and systems
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• v.7 no.2
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• pp.133-137
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• 2003

Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast-based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.

• Ocean and Polar Research
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• v.45 no.1
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• pp.1-9
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• 2023

Marine microalgae have different collection methods depending on their habitat. In the case of adhesive microalgae, it is difficult to separate organisms from the substrate surface, and contamination is likely to occur during the sampling process. In this study, we analyzed the collection efficiency of adhesive microalgae using three artificial fiber materials (nylon, blend fabric, and viscose rayon). Each fiber showed different fiber diameter and pore characteristics (nylon 26.09 ㎛, blend fabric 56.6 ㎛, viscose rayon 101.3 ㎛). In addition, attached organisms were collected on the surface of artificial substrates at Bukseong Port in Incheon using each tested fiber material. After that, we investigated the population and species composition. The highest number of cells was found in nylon, which was 8 times higher than in the least collected viscose rayon material. In addition, we identified 24 microalgal species from the substrate, demonstrating that the species composition differed from that of surface water. The number of collected microalgae species varied depending on the fiber materials, with nylon containing all the adhesive microalgae. In contrast, only a few microalgae were observed in other fibers. These results suggest that, of the tested fibers, nylon material may be suitable for collecting adhesive microalgae. As a result, this study may be useful for future research on adhesive microalgae.

• Korean Journal of Ichthyology
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• v.18 no.4
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• pp.293-299
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• 2006

Copper,zinc superoxide dismutase (Cu,Zn-SOD) plays a key role to the first antioxidant defense system against oxidative stress in diverse aerobic organisms. Due to the housekeeping action of Cu,Zn-SOD, it was reported that the structure and function have been conserved during evolution. In this study Cu,Zn-SOD from cloudy catshark Scyliorhinus torazame was subjected to phylogenetic analyses to know its evolutionary relationship in the vertebrate lineage. Molecular phylogenetic trees inferred by NJ, MP, ML and/or Bayesian analyses showed two shark species, Prionace glauca and S. torazame grouped together with high statistical supports. In general, they placed at the separated position from bony vertebrates. Thereafter, bony vertebrates composed of teleosts and birds/mammals (amniotes) formed a monophyletic group. Each teleost and amniote clade was also supported by relatively high statistical values. These phylogenetic relationships are well congruent with the phylogenetic hypothesis of the ancestral position of cartilaginous fishes to bony vertebrates.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.26 no.1
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• pp.14-19
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• 1990

This paper describes on the characteristics of the acoustic echoes from the scattering layers by the aggregation of marine organisms which was measured by using a 50kHz echo sounder on board of the training ship SAEBADA belong to National Fisheries University of Pusan during the summer, 1989 in the East China Sea. A data acquisition system was used to record digitally the envelope of the echoes and the frequency distribution of echo amplitudes on the whole water column was examined as a function of time during the sunrise and sunset periods. Acoustic data showed that the abundance distribution of marine organisms according to depth in the servery area changed with time and that the organisms were most active during the twilight of morning, and the echo strength on the water column or scattering layer was also strongest during this time. Additional results of this survey suggest that other interest, such as the relationship between environmental conditions and biomass depth distribution, could be also investigated in hydroacoustic method.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.4
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• pp.305-311
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• 1993

Marine organisms were investigated to identify the marine actinomycetes that produced noble bioactive compounds. Microorganism counts range from $2.1{\times}10^3\;to\;1.2{\times}10\;CFU/g$ of marine organisms. Actinomycetes constituted 0.01 to $0.5\%$ of culturable microbial community. We identified the marine actinomycetes that produced novel bioactive compounds. During the course of screening for bioactives from the marine microorganisms, we found that the strain in sponge had antimicrobial activities. From the morphological, cultural and various physiological characteristics, this strain was identified for Actinomycetes No. 101. The optimal compositions of culture medium for Actinomycetes No. 101 were starch 30g/l as carbon source, casamino acid 10g/l as nitrogen source. The optimal pH of medium and fermentation temperature were $6.5{\sim}7.0$ and $30^{\circ}C$, respectively. Fermentation has been conducted in the marine broth at $30^{\circ}C$ for 72 hour. The yield of fermentation got about 3g as dry weight(per liter of broth). The distribution of antimicrobial activity of Actinomycetes No. 101 was screened by paper disc. The extract of cultured cell and broth inhibited the growth of Staphylococcus aureus and Bacillus subtilis, but the inhibition action was week against yeast and mold.

• Journal of the Korean Society of Marine Environment & Safety
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• v.29 no.5
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• pp.456-461
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• 2023

The Korea Maritime Environment Corporation is conducting a comprehensive survey of the national marine ecosystem under the commission of the Ministry of Oceans and Fisheries (MOF) to ensure continuous use of the ocean, preserve and manage the marine ecosystem. The survey has set major peaks to investigate changes in the marine ecosystem around the Korean Peninsula. However as the peak has been set around the coast, it is necessary to expand the scope of investigation to encompass offshore areas. Meanwhile, the Aids to Navigation Division of the MOF supports a comprehensive national marine ecosystem survey providing photographs of fouling organisms during the Aids to Navigation lifting inspection, however, the photographs are provided only in consultation with the Korea Maritime Environment Corporation. Therefore, a study was conducted to generate information on fouling organisms using deep learning-based image processing algorithms by the lifting Aids to Navigation and dorsal buoys so that Aids to Navigation could be used as the major component of a comprehensive national marine ecosystem. If the Aids to Navigation are used as the peak of the survey, they could serve as fundamental data to enhance their own value as well as analyze abnormal marine conditions and ecosystem changes in Korea.

• The Korean Journal of Malacology
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• v.31 no.3
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• pp.203-212
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• 2015

This study was performed to describe the influence of temperature on the clearance rate and ingestion rate of the blue mussel, Mytilus galloprovincialis with three food organisms and habitat location (shell size) of mussel. Food organisms used in this experiments were Isochrysis galbana, Chaetoceros didymus and Prorocentrum dentatum. The size of mussels inhabiting higher midlittoral zone was smaller than those of lower midlittoral zone. Regardless of the kind of food organisms, filtration rates and ingestion rates of higher midlittoral mussels were higher than those of lower midlittoral mussels in experiment temperature conditions. The variation of filtration rate and ingestion rate showed same tendency with temperature. Filtration rates and ingestion rates increased with temperature, and recorded maximum values at $20-25^{\circ}C$ of temperature, and thereafter decreased gradually. Theoretical optimum temperatures showing maximum filtration rates and ingestion rates estimated from polynomial regression curves were also in the range of $20-25^{\circ}C$. Blue mussels showed different variation of filtration rate and ingestion rate with the kind of food organisms. Filtration rates and ingestion rate based on cell number were similar regardless of habitat location(tidal elevation) and food organisms. Ingestion rates based on carbon content showed very high values in case of P. dentatum beside I. galbana and C. didymus as food organism.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.6 no.3
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• pp.383-391
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• 2016

Ballast water provides stability and manoeuvrability to a ship. Foreign harmful aquatic organisms, which were transferred by ballast water, cause disturbing ecosystem. In order to minimize transference of foreign harmful aquatic organisms, IMO(International Maritime Organization) adopted the International Convention for the Control and Management of Ship's Ballast Water and Sediments in 2004. If the convention take effect, a port authority might need to check that ballast water is properly disposed of. In this paper, we propose a method of counting harmful aquatic organisms in ballast water thorough image processing. We extracted three samples from the ballast water that had been collected at Busan port in Korea. Then we made three grey-scale images from each sample as experimental data. We made a comparison between the proposed method and CellProfiler which is a well known cell-counting program based on image processing. Setting of CellProfiler is empirically chosen from the result of cell count by an expert. After finding a proper threshold for each image at which the result is similar to that of CellProfiler, we used the average value as the final threshold. Our experimental results showed that the proposed method is simple but about ten times faster than CellProfiler without loss of the output quality.

• Journal of Ecology and Environment
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• v.40 no.3
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• pp.136-143
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• 2016

Background: Mussels are stubborn organisms attached to solid substrata by means of byssus threads. The abundance of marine mussel Mytilus edulis in marine facilities like power stations was reason to select among fouling animals. Methods: Mortality patterns as well as physiological behavior (oxygen consumption, foot activity, and byssus thread production) of two different size groups (14- and 25-mm shell length) of M. edulis were studied at different hydrogen peroxide concentrations ($1-4mg\;l^{-1}$). Results: Studied mussels showed progressive reduction in physiological activities as the hydrogen peroxide concentration increased. Mussel mortality was tested in 30 days exposure, and 14 mm mussels reached the highest percentage of 90% while 25 mm mussels reached 81%. Produced data was echoed by Chick-Watson model extracted equation. Conclusions: This study points that, while it could affect the mussel mortality moderately in its low concentrations, hydrogen peroxide has a strong influence on mussels' physiological activities related to colonization. Therefore, hydrogen peroxide can be an alternative for preventing mussel colonization on facilities of marine environment.

• Journal of Food Hygiene and Safety
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• v.10 no.2
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• pp.73-79
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• 1995

Incidents of seafood and massive fish kills have been rapidly increasing in both frequency and geographical distribution and the socioeconomic impacts brought by those incidents. However, the biological origins of those marine toxins have not been well clarified. Most of the marine organisms investigated are filter-feeder, which accumulate toxins from their food and/or their symbiotic microalgae. We have examined the action on rabbit platelets of marine toxins isolated from cultured dinoflagellates and sponge collected at Okinawa. Maitotoxin (MTX) is a water-soluble toxin isolated from the cultured dinoflagellate Gambierdiscus toxicus which causes a seafood poisoning in tropical regions. Zooxanthellatoxin A (ZT-A) was isolated from exteracts of cultured symbiotic dinoflagellate Symbiodinium sp. (socalled zooxanthella) from flatworms of the genus Amphiscolops collected at Okinawan marine sponge Theonella sp. MTX caused a disaggregation and a dissolution of large aggregates. ZT-A caused a dissolution of small aggregates followed by a increment of light tranmission. TZ-A caused an initial and transient shape change followed by a sustained aggregation and a increment of large aggregates. In conclusion, marine toxins exert unique patterns on the light trasmission and the size of aggregates in rabbit platelets by their concentrations and kinds