• Ocean Science Journal
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• 제42권1호
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• pp.9-17
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• 2007

The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semi-enclosed, inner area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. $0.4\;mg\;L^{-1}$) and high concentrations of inorganic nutrients (nitrogenous nutrients $>36\;{\mu}M$, phosphate $>4\;{\mu}M$) in the bottom layer. Higher densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed into more than $10^6\;cells\;L^{-1}$ at the surface layer of the inner area, while its abundance was much lower ($10^3-10^4\;cells\;L^{-1}$) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p < 0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to the T. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates.

• Ocean and Polar Research
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• 제33권4호
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• pp.457-472
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• 2011

To elucidate the trophic role of heterotrophic nano- and microplankton (HNMP), we investigated their biomass, community structure, and herbivory in three different water masses, namely, south of Polar Front (SPF), Polar Front Zone (PFZ), the Sub-Antarcitc Front (SAF) in the Drake Passage in the Southern Ocean, during the austral summer in 2002. We observed a spatial difference in the relative importance of the dominant HNMP community in these water masses. Ciliates accounted for 34.7% of the total biomass on an average in the SPF where the concentration of chlorophyll-a was low with the dominance of pico- and nanophytoplankton. Moreover, the importance of ciliates declined from the SPF to the SAF. In contrast, heterotrophic dinoflagellates (HDFs) were the most dominant grazers in the PFZ where the concentration of chlorophyll-a was high with the dominance of net phytoplankton. HNMP biomass ranged from 321.9 to 751.4 $mgCm^{-2}$ and was highest in the PFZ and lowest in the SPF. This result implies that the spatial dynamic of HNMP biomass and community was significantly influenced by the composition and concentration of phytoplankton as a food source. On an average, 75.6%, 94.5%, and 78.9% of the phytoplankton production were consumed by HNMP in the SPF, PFZ, and SAF, respectively. The proportion of phytoplankton grazed by HNMP was largely determined by the composition and biomass of HNMP, as well as the composition of phytoplankton. However, the herbivory of HNMP was one of the most important loss processes affecting the biomass and composition of phytoplankton particularly in the PFZ. Our results suggest that the bulk of the photosynthetically fixed carbon was likely reprocessed by HNMP rather than contributing to the vertical flux in Drake Passage during the austral summer in 2002.

• Animal Systematics, Evolution and Diversity
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• 제28권3호
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• pp.192-207
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• 2012

To supply the morphological and ecological information of oligotrich ciliates in Korea, water samples were seasonally collected in Gwangyang Bay and Jinhae Bay from August, 2010 to February, 2012 and processed by quantitative protargol staining method. As a result, six species belonging to the genus Strombidium Clapar$\grave{e}$de and Lachmann, 1859 are identified: Strombidium emergens Kahl, 1932; S. dalum Lynn et al., 1988; S. epidemum Lynn et al., 1988; S. tressum Lynn et al., 1988; S. bilobum Lynn and Gilron, 1993; S. pollostomum Lynn and Gilron, 1993. These six species were newly reported from Korean coastal waters. Strombidium emergens is a middle sized Strombidium ($20-50{\mu}m$ in length) and has open and deep oral groove to girdle portion. Strombidium dalum is a small sized Strombidium (${\geq}20{\mu}m$ in length), that has torch-like spiral anterior membranelles and an inverted triangles-shaped macronucleus on the posterior pole. The small sized S. epidemum has conspicuous trichites surrounding the girdle portion and ventral membranelles distinctly separated from anterior membranelles. The small sized S. tressum has torch-like spiral and extremely long trichites among the cilia of anterior membranelles. The middle sized S. bilobum has a bilobed macronucleus. Strombidium pollostomum is also a small sized Strombidium but its ventral membranelles are continuously connected with anterior membranelles. The five species except S. emergens occurred frequently over the wide range of water temperatures and salinities.

• 한국어병학회지
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• 제22권1호
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• pp.45-52
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• 2009

Zebrafish was firstly applied to an experimental model for scuticociliatosis caused by Philasterides dicentrarchi, a facultative parasitic ciliate in cultured marine fish. The susceptibility of zebrafish to infection of P. dicentrarchi was assessed by intraperitoneal injection of the ciliates, which produced typical symptoms of scuticociliatosis and significant mortality. The potential use of zebrafish as a model to evaluate the vaccine efficacy against scuticociliatosis was analyzed by immunization of zebrafish with the ciliates lysate. Furthermore, the effect of different adjuvants, such as Quillaja saponin (QS), Montanide, and Freund’s incomplete adjuvant (FIA) on the protective efficacy of the vaccine was investigated. Groups of zebrafish injected with QS or Montanide alone showed higher survival of fish against challenge test compared to control fish. The results suggest that adjuvant-mediated enhancement of innate immune responses play important roles in protection of fish against scuticociliatosis. The considerably high survival in the fish immunized with the antigen alone indicates that the ciliate lysate itself is highly immunogenic to zebrafish, which can elicit protective immune responses. The protective potential of the antigen, ciliate lysate, was enforced through combined administration with adjuvants including QS, Montinide and FIA. No or low mortalities in the groups of fish immunized with the antigen plus adjuvants suggests that the adaptive immune responses of zebrafish might be accelerated by the adjuvants or the protective potential of the antigen and adjuvants might synergistically interact. In spite of several shortcomings such as difficulties in sampling of serum and leucocytes enough to routine immunological analyses, zebrafsih might be the most convenient experimental animal for scuticociliatosis.

• Animal Systematics, Evolution and Diversity
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• 제27권2호
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• pp.115-122
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• 2011

The morphology of the two marine hypotrichous ciliates Apokeronopsis bergeri and A. ovalis, isolated from the Yellow Sea, Korea, are described based on live and protargol-impregnated specimens. It is the first time that these species have been recorded in Korea. In addition, the small subunit ribosomal RNA gene was sequenced for comparison with the public database. The genus Apokeronopsis has recently been established in the family Pseudokeronopsidae, and the two congeners of the Korean population share the following characteristics: one row of one or more buccal cirri; usually two frontoterminal cirri; midventral complex composed of two distinctly separated rows; one left and one right marginal row; number of transverse cirri, more than eight; absence of caudal cirri; two types of cortical granules. Apokeronopsis bergeri differs from A. ovalis primarily in body shape (fusiform vs. oval form), size (usually $260{\times}80{\mu}m$ vs. $160{\times}55{\mu}m$), type II cortical granules (oval vs. round shape; yellow-green vs. mostly colourless and only a few yellow-green in colour), and morphometric data (75-106 vs. 53-70 in adoral membranelles; 37-47 vs. 24-36 in frontal cirri; 9-15 vs. 1-2 in buccal cirri), as well as molecular data (2.87% of pairwise distance).

• Journal of Species Research
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• 제8권4호
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• pp.380-388
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• 2019

We collected ciliate species from diverse habitats (brackish water, marine water, and terrestrial ecosystems) composed of estuarine, coast, and moss samples during 2017-2018 in Korea. The morphology and infraciliature of these species were examined and identified based on the observations of living cells and protargol impregnated specimens. Of the ciliates we identified, we described eight unrecorded species in Korea as followings: class Heterotrichea - Condylostoma vorax, Peritromus kahli; class Protocruziea - Protocruzia labiata; class Spirotrichea - Hemiamphisiella granulifera; class Litostomatea - Loxophyllum chinense; class Phyllopharyngea - Trochilioides recta; and class Oligohymenophorea -Cohnilembus verminus, Uronemella filificum. Among them, Protocruzia labiata is a poorly known species. In this paper, we provide the improved diagnoses and brief descriptions of the species with their microphotographs. The taxonomic classifications of the eight species are summarized as six classes, eight orders, five families, and eight genera. These voucher slides with protargol impregnated specimens have been deposited in the National Institutes.

• ALGAE
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• 제39권3호
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• pp.129-147
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• 2024

The species complex Mesodinium rubrum / major, common red tide-forming ciliates, has been intensively studied with regards to its ecological roles in global marine ecosystems and the evolutionary aspects of its "stolen" organelles (kleptoplasty and karyoklepty). Nonetheless, the taxonomy of the species within the complex remains unclear. A new marine Mesodinium species isolated from Gomso Bay, Korea, was cultivated under mixotrophic conditions by providing Teleaulax amphioxeia, a red cryptomonad, as prey. Cells of the new isolate consisted of two portions separated by two types of polykinetids. The number of polykinetid associated with the equatorial ciliary belt was approximately 38, and each consisting of two rows of up to 18 alternating kinetosomes each. There was an equal number of cirral polykinetids, each consisting of 16 kinetosomes organized into four longitudinal rows having five, five, four, and two kinetosomes, respectively (in anti-clockwise direction). The two kinds of kinetids and their associated microtubules and fibers were structurally similar to those of M. rubrum from Denmark. However, the Korean Mesodinium species was characterized by its broad posterior portion, 20-22 tentacles, and a cytopharyngeal annulus. Molecular phylogeny based on internal transcribed spacer sequences placed the Korean isolate in clade B of the M. rubrum / major species complex, rather than in clade F representing the neotype of M. rubrum. Based on morphological, ultrastructural, and molecular data, we propose the Korean strain as a new marine Mesodinium species, M. annulatum.

• 환경생물
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• 제33권4호
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• pp.403-411
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• 2015

본 연구는 외부 해수가 계속적으로 유입되는 개방형 인공생태계와 해수의 유입이 없는 폐쇄형 인공생태계에서 미소생태계의 변화를 파악하고자 실험실 내에서 100 L 크기의 인공해양소형생태계 연구를 수행하였다. 수온은 폐쇄형 및 개방형 인공생태계에서 큰 차이가 없었다. 염분은 폐쇄형 생태계에서 수체의 증발에 따라 증가를 보였고 용존산소 및 용존무기질소 농도는 폐쇄형에서 감소하는 반면, 개방형에서는 초기농도와 큰 차이를 보이지 않았다. 용존무기인 및 용존규소는 두 시스템에서 차이가 없었다. 식물플랑크톤은 폐쇄형에서 감소를 하였던 반면, 개방형에서는 증가 양상을 보였으나 Autotrophic nanoflagellates는 식물플랑크톤 개체수의 변동과 반대되는 양상을 보였다. 타가영양세균은 폐쇄형에서 증가하는 양상을 보였고, 이와 함께 heterotrophic nanoflagellates 및 섬모충이 시간차를 두어 증가하는 양상을 보였다. 그러나, 개방형 인공생태계에서는 특이한 변화를 나타내지 않았다. 결론적으로, 폐쇄형 인공생태계와 개방형 인공생태계에서 미소생물상 및 환경요인들의 변화의 연구는 향후 연구자들이 인공생태계 연구에 있어서 기초자료로 활용할 수 있을 것으로 기대하며, 신뢰성 있는 인공생태계 연구를 수행할 수 있을 것으로 판단된다.

• Journal of the korean society of oceanography
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• 제37권4호
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• pp.201-211
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• 2002

We investigated the abundance and biomass of dinoflagellates and factors controlling their abundance in marine planktonic ecosystems in Korean coastal waters. The abundance of photosynthetic (PDNF) and heterotrophic dinoflagellates (HDNF) was in the range of 0.7${\times}$10$^2$ cells/1-14.0${\times}$10$^6$ cells/1 and in the range of 3.0${\times}$10$^2$ cells/1-6.47${\times}$10$^5$ cells/I, respectively. Their biomass was 0.5${\times}$10$^{-1}$-2.56${\times}$10$^4$ ${\mu}gC/I$ and 2.0${\times}$10$^{-1}$-1.5${\times}$10$^{2}$ ${\mu}gC/I$, respectively. In order to find factors controlling their abundance, stepwise regression and best subsets regression analyses were used. We found that during the summer the most important factors controlling PDNF abundance are DO, P, N and S (abiotic factors), and for HDNF, the abundance of zooplankton, ciliates and HF (biotic factors), and that high turbidity may effect the distribution of dinoflagellate species.

• Ocean and Polar Research
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• 제31권1호
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• pp.77-95
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• 2009

Ciliate abundance and biomass were determined at three anchored stations in the Han River estuary trimonthly from May 2006 to February 2008. The total abundance of ciliates ranged from 0-13,853 cells $l^{-1}$ and biomass ranged from $0.00-73.98\;ugC\;l^{-1}$. Owing to the large seasonal temperature variation (range 0.20-$27.2^{\circ}C$), ciliate abundance displayed marked seasonal variation, being higher in summer than in other seasons. Tintinnids dominated the ciliate communities at station YC where suspended sediment was highest. Difference of ciliate biomass between ebb tide and flood tide were irregular. Seasonal variations of ciliate carbon biomass at each station were related to temperature, suspended sediment and chl.a, especially nano chl.a. Geographical variations of ciliate carbon biomass in each season were related to suspended sediment and salinity.