• Title/Summary/Keyword: marine biotechnology

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Production of Bioemulsifier from a Marine Bacterium Achromobacter sp. M-1220 (해양세균 Achromobacter sp. M-1220 균주를 이용한 생물유화제 물질의 생산)

  • 박중연;홍용기
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.252-256
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    • 1989
  • A marine bacterium which was isolated from the enrichment culture for the emulsification of Bunker-C oil produced a bioemulsifier potently. The strain identified as an Achromobacter sp. M-1220. The bioemulsifier was produced during mid-logarithmic phase in hexadecane oil medium at 18$^{\circ}C$. It appeared to be a cationic peptidolipid substance and showed an active stabilizing effect on the emulsion of crude oils and a few vegetable oils.

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New record of a sea star of genus Solaster (Asteroidea: Valvatida: Solasteridae) from the East Sea, Korea

  • Ubagan, Michael Dadole;Shin, Sook
    • Korean Journal of Environmental Biology
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    • v.37 no.4
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    • pp.503-507
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    • 2019
  • Asteroid specimens were collected using fishing nets from the East Sea, Korea. The specimens were identified as Solaster paxillatus Sladen, 1889, belonging to the family Solasteridae of the order Valvatida. This species can be distinguished from three other Korean Solaster species by having 10 arms; subambulacral spines comprising four or five near base of arm, six or seven in middle part, and five or six in distal part of arm; furrow spines comprising four or five near basal part and three or four in distal part of arm; and suboral spines comprising more than ten spinules. The morphological characteristics of this species were described with illustrations. Solaster paxillatus is first reported in marine fauna of Korea.

Enzyme-Linked, Biotin-Streptavidin Bacterial-Adhesion Assay for Helicobacter pylori Lectin-Like Interactions with Cultured Cells

  • Murillo, Guzman;Antonia, Maria;Ascencio, Felipe
    • Journal of Microbiology and Biotechnology
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    • v.11 no.1
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    • pp.35-39
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    • 2001
  • A simple method for studying the lectin-like interactions between Helicobacter pylori and cultured human epithelial cell lines was developed using an enzyme-linked, biotin-streptavidin bacterial-adhesion assay. The present study suggests that this method is suitable for evaluating the participation of lectin interactions in the adhesion of H. pylori to cultured HeLa S3 and Kato III cells, both fixed and glycosidase-treated cells, as well as assessing glycoconjugated binding inhibition studies. The time-course and dose-dependent kinetics of the biotin-labeled H. pylori adhesion th the formaldehyde-fixed Hela S3 and Kato III cell lines exhibited saturation. In addition, the binding of the biotin-labeled H. pylori to the formaldehyde-fixed cultured cells was partially blocked by pre-incubation with glycoconjugates and polyclonal antibodies against a heparan sulfate binding protein from H. pylori.

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Radical Scavenging Hydroxyphenyl Ethanoic Acid Derivatives from a Marine-Derived Fungus

  • Li Xifeng;Kim Se-Kwon;Kang Jung-Sook;Choi Hong-Dae;Son Byeng-Wha
    • Journal of Microbiology and Biotechnology
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    • v.16 no.4
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    • pp.637-638
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    • 2006
  • Bioassay-guided fractionation of an organic extract of the culture broth from an unidentified marine-derived fungus led to the isolation of a new metabolite, N-[2-(4-hydroxyphenyl) acetyl]formamide (1), along with four known polyketides, 4-hydroxyphenyl acetamide (2), 4-hydroxyphenyl acetic acid (3), 3,4-dihydroxyphenyl acetic acid (4), and N-[2-(4-hydroxyphenyl)ethenyl]formamide (5). The structures of 1-5 were elucidated by spectral data analyses. Among them, compounds 1, 4, and 5 exhibited significant radical scavenging activity against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) with $IC_{50}$ values of 8.4, 11.9, and $0.2{\mu}M$, respectively.

Redoxcitrinin, a Biogenetic Precursor of Citrinin from Marine Isolate of Fungus Penicillium sp.

  • Zhang, Dahai;Li, Xianguo;Kang, Jung-Sook;Choi, Hong-Dae;Jung, Jee-H.;Son, Byeng-Wha
    • Journal of Microbiology and Biotechnology
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    • v.17 no.5
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    • pp.865-867
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    • 2007
  • A chemical analysis of the fermentation of the marine-derived fungus Penicillium sp. led to the isolation of a biogenetic precursor of citrinin, redoxcitrinin(1), together with polyketide mycotoxins, phenol A(2), citrinin H2(3), 4-hydroxymellein(4), citrinin(5), and phenol A acid(6). The structures of compounds 1-6 were determined on the basis of physicochemical data analyses. Among them, compounds 1-3 exhibited a potent radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl(DPPH) with $IC_{50}$ values of 27.7, 23.4, and $27.2{\mu}M$, respectively.

Fisheries Resources -A Brief Introduction of the Republic of Senegal

  • Mbengue, Modou
    • Journal of Marine Bioscience and Biotechnology
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    • v.3 no.1
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    • pp.38-41
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    • 2008
  • Fish is a major source of protein for the Senegalese population. Fishing plays a dominant role in the Government's policy towards generating employment. It currently generates about 100,000 direct jobs (fishermen) for nationals, of which more than 90% are in small-scale fishing. The fishing industry also contributes to Government revenue through different agreements. In addition to associated dues, fishing agreements imply a series of economic, trade and technical counterparts. Under the latest fishing agreement concluded by Senegal and the European Union (1997-2001), direct financial compensation amounts to about CFAF 32 billion. Despite its economic and social importance, the sector has to face serious disequilibria both in resource exploitation and market supply: the coastal demersal (deep lying fish) stocks with high market value (mostly exported) are fully and even over-exploited, with a serious risk of local market supply shortages looming ahead as the fishing effort shifts from locally consumed species to export-oriented ones.

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New records of three endophytic green algae from Grateloupia spp. (Rhodophyta) in Korea

  • Kim, Chansong;Kim, Young Sik;Choi, Han Gil;Nam, Ki Wan
    • ALGAE
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    • v.29 no.2
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    • pp.127-136
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    • 2014
  • Endophytic green algae growing in fronds of Grateloupia spp. were examined for infection frequency from their field populations of Jeju, Wando, and Uljin, Korea in August and September 2013. Three endophytes were isolated in laboratory culture from a G. lanceolata thallus collected in Jeju. Unialgal cultures were made from the endophytes, and their morphological characteristics were observed with light microscopy. In addition, a phylogenetic analysis based on chloroplast-encoded elongation factor tufA gene sequences was performed to identify the G. lanceolata endophytes. Three filamentous green endophytic species, Ulvella leptochaete, Blastophysa rhizopus, and Bolbocoleon piliferum were reported for the first time in Korea. General biological information for the three endophytes was also described.

The Sediment-Water Interface Increment due to the Complex Burrows of Macrofauna in a Tidal Flat

  • Koo, Bon-Joo;Kwon, Kae-Kyoung;Hyun, Jung-Ho
    • Ocean Science Journal
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    • v.40 no.4
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    • pp.221-227
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    • 2005
  • The architecture of macrofaunal burrows and the total area of the sediment-water interface created by biogenic structure were investigated in the Donggeomdo tidal flat on the west coast of Korea. Resin casting methods were applied to recover burrows of four dominant species, Macrophthalmus japonicus, Cleistostoma dilatatum, Perinereis aibuhitensis, and Periserrula leucophryna, and whole burrows within the casting area at three sites in different tidal levels. P. leucophryna excavated the largest burrow in terms of a surface area among them. In the case of whole burrow casting, the space occupied by the biogenic structure was extended into deeper and expanded more greatly at the higher tidal level. In the uppermost flat, the burrow wall surface area within sediment was more extensive than the sediment surface area. Increased oxygen supply through the extended interface could enhance the degradation rates of organic carbon and also change the pathways of degradation. Quantifying the relationship between the extended interface and mineralization rate and pathway requires more extensive study.

Genetic Variation of the Three Pampus spp. (Pisces: Stromateidae) using Amplified Fragment Length Polymorphism (AFLP) (AFLP 분석에 의한 병어속 (Pampus) 3종의 유전 변이)

  • Yoon, Young-Eun;Park, Sang-Yong;Bae, Joo-Seung;Bang, In-Chul
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.2
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    • pp.146-150
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    • 2009
  • Genetic variation and relationship of two wild (Pampus argenteus and P. echinogaster) and one cultured (P. chinesis) pomfret fish belonging to the genus Pampus were assessed. Specimens were collected from Korea and China and subjected to amplified fragment length polymorphism (AFLP) DNA fingerprinting. Four primer combinations generated a total of 304 DNA fragments ranging from 153 to 251 bands. Polymorphism and genetic diversity of cultured P. chinensis (22.9% and 0.038) were significantly lower than the two wild species of P. argenteus (93.6% and 0.311) and P. echinogaster (94.0% and 0.290). Genetic distance ranged from 0.335 (P. argenteus and P. echinogaster) to 0.646 (P. argenteus and P. chinensis) and showed a congeneric relationship within this genus. Twenty one of specific AFLP markers from four primer combinations bands were produced. These results suggest that AFLP polymorphism may be a useful marker for genetic identification among the three species studies here.