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A Study on the Successful Operation of B2B e-Marketplaces in the Korea Industry (한국산업의 B2B e-Marketplace 성공적 운영 관한 연구)

  • Lee, Jae-Kyu;Shin, Seung-Man
    • International Commerce and Information Review
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    • v.9 no.3
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    • pp.59-79
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    • 2007
  • This paper is to identify whether the mechanism of the real market as an off-line market developed from the transaction cost theory from previous research is applied to on-line market, such as usiness to Business e-marketplaces. With three factors(parties, product, environments) selected from previous research, four cases picked up from all B2B e-marketplace sites as of April 2007, in the view of Business to Business Matrix which presented by Kaplan & Sawhney(2000), were studied. They were accompanied by interview with CEO and team manager, and they explained their business and revenue model of the company. From the interview, the relation between three factors and usiness to Business e-marketplace was observed by taking sub-factors into consideration; parties -asset specificity, information asymmetry, product factors - standardization, price stability, and environments factors - competition, market uncertainty. The implications of this study are to analyze the relation of the transaction theory in offline and online. Also, this is the first study that analyzed it. In the future, another research based on this research will be studied.

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Fine Mutational Analysis of 2B8 and 3H7 Tag Epitopes with Corresponding Specific Monoclonal Antibodies

  • Kim, Tae-Lim;Cho, Man-Ho;Sangsawang, Kanidta;Bhoo, Seong Hee
    • Molecules and Cells
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    • v.39 no.6
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    • pp.460-467
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    • 2016
  • Bacteriophytochromes are phytochrome-like light-sensing photoreceptors that use biliverdin as a chromophore. To study the biochemical properties of the Deinococcus radiodurans bacteriophytochrome (DrBphP) protein, two anti-DrBphP mouse monoclonal antibodies (2B8 and 3H7) were generated. Their specific epitopes were identified in our previous report. We present here fine epitope mapping of these two antibodies by using truncation and substitution of original epitope sequences in order to identify minimized epitope peptides. The previously reported original epitope sequences for 2B8 and 3H7 were truncated from both sides. Our analysis showed that the minimal peptide sequence lengths for 2B8 and 3H7 antibodies were nine amino acids (RDPLPFFPP) and six amino acids (PGEIEE), respectively. We further characterized these peptides in order to investigate their reactivity after single deletion and single substitution of the original peptides. We found that single-substituted 2B8 epitope (RDPLPAFPP) and dual-substituted 3H7 epitope (PGEIAD) showed significantly increased reactivity. These two antibodies with high reactivity for the short modified peptide sequences are valueble for developing new peptide tags for protein research.

Development of Pretreatment Method for Analysis of Vitamin B12 in Cereal Infant Formula using Immunoaffinity Chromatography and High-Performance Liquid Chromatography

  • Park, Jung Min;Koh, Jong Ho;Kim, Jin Man
    • Food Science of Animal Resources
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    • v.41 no.2
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    • pp.335-342
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    • 2021
  • Vitamin B12 deficiency may lead to serious health issues in both infants and adults. A simple analytical method involving sample pretreatment with enzyme, followed by cyanide addition under acidic conditions; separation on an immunoaffinity column; and high-performance liquid chromatography (HPLC) was developed for the rapid detection and quantitation of vitamin B12 in powdered milk. Detection limit and powdered milk recovery were determined by quantitative analysis. The limits of detection and quantitation were 2.71 and 8.21 ㎍/L, respectively. Relative standard deviations of the intra-day and inter-day precisions varied in the ranges of 0.98%-5.31% and 2.16%-3.90%, respectively. Recovery of the analysis varied in the range of 83.41%-106.57%, suggesting that the values were acceptable. Additionally, vitamin B12 content and recovery in SRM 1849a were 54.10 ㎍/kg and 112.24%, respectively. Our results suggested that the analytical method, including the sample pretreatment step, was valid. This analytical method can be implemented in many laboratory-scale experiments that seek to save time and labor. Therefore, this study shows that immunoaffinity-HPLC/ultraviolet is an acceptable technique for constructing a reliable database on vitamin B12 in powdered milk containing starch as well as protein and/or fat in high amounts.

Properties of SiC-$ZrB_2$ Electroconductive Ceramic Composites by Spark Plasma Sintering (방전플라즈마 소결에 의한 SiC-$ZrB_2$ 도전성 세라믹 복합체 특성)

  • Shin, Yong-Deok;Ju, Jin-Young;Jo, Sung-Man;Lee, Jung-Hoon;Kim, Cheol-Ho;Lee, Hee-Seung
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.1252_1253
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    • 2009
  • The composites were fabricated by adding 0, 15, 20, 25[vol.%] Zirconium Diboride(hereafter, $ZrB_2$) powders as a second phase to Silicon Carbide(hereafter, SiC) matrix. The physical, mechanical and electrical properties of electroconductive SiC ceramic composites by spark plasma sintering(hereafter, SPS) were examined. Reactions between $\beta$-SiC and $ZrB_2$ were not observed in the XRD analysis The relative density of mono SiC, SiC+15[vol.%]$ZrB_2$, SiC+20[vol.%]$ZrB_2$ and SiC+25[vol.%]$ZrB_2$ composites are 90.97[%], 74.62[%], 77.99[%] and 72.61[%] respectively. The XRD phase analysis of the electroconductive SiC ceramic composites reveals high of SiC and $ZrB_2$ and low of ZrO2 phase. The electrical resistivity of mono SiC, SiC+15[vol.%]$ZrB_2$, SiC+20[vol.%]$ZrB_2$ and SiC+25[vol.%]$ZrB_2$ composites are $4.57{\times}10^{-1}$, $2.13{\times}10^{-1}$, $1.53{\times}10^{-1}$ and $6.37{\times}10^{-2}[{\Omega}{\cdot}cm]$ at room temperature, respectively. The electrical resistivity of mono SiC, SiC+15[vol.%]$ZrB_2$, SiC+20[vol.%]$ZrB_2$ and SiC+25[vol.%]$ZrB_2$ are Negative Temperature Coefficient Resistance(hereafter, NTCR) in temperature ranges from 25[$^{\circ}C$] to 100[$^{\circ}C$]. It is convinced that SiC+20[vol.%]$ZrB_2$ composite by SPS can be applied for heater above 1000[$^{\circ}C$].

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Workers' Exposure to Airborne Fibers in the Man-made Mineral Fibers Producing and Using Industries (인조광물섬유 제품 제조 및 취급 근로자의 공기중 섬유 노출 평가 및 노동부 노출기준 고찰)

  • Shin, Yong Chul;Yi, Gwang Yong
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.15 no.3
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    • pp.221-231
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    • 2005
  • In this study, occupational exposures to man-made mineral fibers (MMMFs) including glass wool, rock wool, and continuous glass filament fibers were determined and evaluated on the American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value (TLV). A total of 171 personal samples collected from 4 glass wool fiber, 2 rock wool fibers, 4 continuous filament glass fiber products manufacturing and a glass fiber and rock wool insulations using industries, and determined respirable fibers concentrations using the National Institute for Occupational Safety and Health (NIOSH) Method 7400, "B counting rule. The fiber concentrations of samples from workers installing thermal insulations in a MMMF using industry showed the highest value: geometric mean (GM) = 0.73 f/cc and maximum = 2.9 f/cc, 70% of them were above the TLV, 1 f/cc. Workers' exposure level (GM= 0.032 f/cc) in the rock wool manufacturing industries was significantly higher than those of glass wool (GM=0.012 f/cc) and continuous filament glass fibers (GM=0.010 f/cc) manufacturing industries (p<0.01). No samples were more than the TLV in the MMMF manufacturing industries. There was a significant difference among companies in airborne fiber levels.

General Pharmacology of CJC-50100, a Recombinant Hepatitis B Virus Surface Antigen (유전자 재조합 B형 간염 바이러스 표면 항원, CJC-50100의 일반약리작용)

  • 정성학;최재묵;이남중;전형수;김연희;김재승;하석훈;김영훈;이나경
    • Biomolecules & Therapeutics
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    • v.9 no.1
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    • pp.55-62
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    • 2001
  • CJC-50100 is a recombinant hepatitis B virus surface antigen (HBsAg) expressed in yeast. The general pharmacological properties of CJC-50100 were evaluated in mice, rats, dogs and isolated guinea pig ileum. The doses were 0.33~33.3 $\mu$g/kg i.m. for mice and rats and 3.3~9.9 $\mu$g/kg i.v. for dogs. The concentrations of 0.002~0.02 $\mu$g/ml were used for the assay with guinea pig ileum. Intramuscular administration of CJC-50100 at the doses did not alter general behavior and the responses for central nervous system, smooth muscle, gastrointestinal system, cardiovascular and respiratory system, and water and electrolytes excretion. In summary, CJC-50100 had no pharmacological effect in these studies even up to the 100-fold of the expected clinical dose, 20 $\mu$g/man/60 kg.

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Least Square B-Spline Fitting For Surface Measurement (곡면 측정을 위한 최소 자승 비-스플라인 Fitting)

  • Jung, Jong-Yun;Lisheng Li;Lee, Choon-Man;Chung, Won-Jee
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.12 no.2
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    • pp.79-85
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    • 2003
  • An algorithm for fitting with Least Square is a traditional and an effective method in processing with experimental data. Due to the lack of definite representation, it is difficult to fit measured data with free curves or surfaces. B-Spline is usefully utilized to express free curves and surfaces with a few parameters. This paper presents the combination of these two techniques to process the point data measured from CMM and other similar instruments. This research shows tests and comparison of the simulation results from two techniques.

RELATIONS AMONG THE FIRST VARIATION, THE CONVOLUTIONS AND THE GENERALIZED FOURIER-GAUSS TRANSFORMS

  • Im, Man-Kyu;Ji, Un-Cig;Park, Yoon-Jung
    • Bulletin of the Korean Mathematical Society
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    • v.48 no.2
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    • pp.291-302
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    • 2011
  • We first study the generalized Fourier-Gauss transforms of functionals defined on the complexification $\cal{B}_C$ of an abstract Wiener space ($\cal{H}$, $\cal{B}$, ${\nu}$). Secondly, we introduce a new class of convolution products of functionals defined on $\cal{B}_C$ and study several properties of the convolutions. Then we study various relations among the first variation the convolutions, and the generalized Fourier-Gauss transforms.

$^{11}B$ Quadrupole Interaction Studies of Boron-doped Graphite Electrode for Lithium Secondary Battery

  • Lee, Youngil;Han, Duk-Young;Lee, Donghoon;Woo, Ae-Ja;Lee, Sam-Hyeon;Kim, Kyung-Han;Lee, Man-Ho
    • Journal of the Korean Magnetic Resonance Society
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    • v.3 no.2
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    • pp.90-99
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    • 1999
  • Doping of boron atoms in graphite has been well known method to increase the discharge capacity as the negative electrode material for lithium secondary battery. Herein, the boron-doped graphites are prepared by mixing 1, 2.5, 5, and 7 wt. % of boron carbide in carbon during the graphitizing process. The structural states of boron in boron-doped graphites are investigated by solid-state 11B NMR spectroscopy. The resonance lines for substitutional boron atoms are identified as the second order quadrupolar powder pattern with the quardrupole coupling constant, QCC = 3.36(2) MHz. The quantitative analysis of 11B NMR spectra with boron-doped graphite has also been performed via simulation.

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Microwave-assisted Sintering of Amorphous Powders

  • Lee, Hoi-Kwan;Kim, Ki-Min;Park, Man-Kyu;Kang, Won-Ho
    • Journal of the Korean Ceramic Society
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    • v.48 no.1
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    • pp.14-19
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    • 2011
  • Microwave heating (MW) was studied for the sintering of amorphous powders. In comparison to conventional heating (CV), the results show that microwave heating has the potential to substantially accelerate the sintering process and allow for highly densified solidification by eliminating the pores and increasing the shrinkage rate. In the heat treatment to synthesize aluminum borate ($Al_{18}B_4O_{33}$) whiskers from precursors, it was found that microwave heating helps the formation of $Al_{18}B_4O_{33}$ crystal as well as the decrease of crystallization temperature of $Al_{18}B_4O_{33}$ to a level significantly lower than the previously reported value of $1050^{\circ}C$.