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Comparison of Required and Additional Man Power's Implemental Task Elements between Present and Future-oriented Duties of School Nutrition Teachers (영양교사의 현재와 미래지향적 직무에서 필수 업무와 추가 인력을 통해 수행 가능한 업무의 비교)

  • Lee, Ho-Jin;Kim, Youngshin;Kim, Seoyoung;Cha, Jina;Ham, Sunny
    • Journal of the Korean Dietetic Association
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    • v.23 no.2
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    • pp.155-179
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    • 2017
  • The purpose of this study was to compare the task elements categorized into required and additional man power's implemental between present and future-oriented duties of school nutrition teachers. The survey consisted of five duties, 27 tasks, 93 task elements, and 270 work details in the task elements of school nutrition teachers. A pilot-test was first conducted on nutrition teachers to confirm the survey contents, and then a main survey was performed on 240 school nutrition teachers, using a self-administrated online method, from July 16 to September 5, 2016. To compare present and future-oriented tasks, frequency analyses were conducted. Work details in the task elements were categorized into 'required' and 'additional man power's implemental', depending on school nutrition teachers' responses, based on a 50% cut-off percentage. The results showed that 13 work details among 60 work details (21.7%) in the 'Duty C. Safety and hygiene management of school foodservice', and 15 work details out of 106 work details (14.2%) in 'Duty B. Foodservice management practices' were identified as additional man power's implemental in future oriented duties. As to 'Duty A. Nutrition management', only three work details among 55 work details (5.5%) were identified as additional man power's implemental. On the other hand, all work details in 'Duty D. Nutrition diet education and counseling and 'Duty E. Reinforce professionalism' were identified as "required" as school nutrition teachers' duties. These findings imply that school nutrition teachers perceive nutrition management and education as their primary duties to the fulfill school foodservice' mission of promoting students' health and fostering students' dietary behaviors. The study offers practical and governmental implications, which can encourage school nutrition teachers to perform their primary duties.

Characterization of Leuconostoc mesenteroides B-742CB Dextransucrase Expressed in Escherichia coli

  • Park, Mi-Ran;Ryu, Hwa-Ja;Kim, Do-Man;Choe, Jun-Yong;John F. Robyt
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.628-635
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    • 2001
  • Recombinant E. coli DH5$\alpha$ harboring a dextransucrase gene (dsrB742) produced an extracellular dextransucrase in a 2% sucrose medium. The enzyme was purified by DEAE-Sepharose and Phenyl-Sepharose column chromatographies upto a 142.97-fold purification with a 11.11% recovery to near homogeneity. The enzyme had a calculated molecular mass of 168.6 kDa, which was in good agreement with the activity band of 170 kDa on a nondenaturing SDS-PAGE. An expression plasmid was constructed by inserting the dsrB742 into a pRSET expression vector. The activity after expression in E. coli BL21(DE3)pLysS increased about 6.7-fold compared to the extracellular dextransucrase from L. mesenteroides B-742CB. The expressed and purified enzyme from the clone showed similar biochemical properties (acceptor reaction, size of active dextransucrase, optimum pH, and temperature) to B-742CB dextransucrase, however, the ability to synthesize ${\alpha}$-(1$\rightarrow$3) branching decreased in comparison to that of L. mesenteroides B-742CB dextransucrase.

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A case of anemia caused by combined vitamin B12 and iron deficiency manifesting as short stature and delayed puberty

  • Song, Seung-Min;Bae, Keun-Wook;Yoon, Hoi-Soo;Im, Ho-Joon;Seo, Jong-Jin
    • Clinical and Experimental Pediatrics
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    • v.53 no.5
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    • pp.661-665
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    • 2010
  • Anemia caused by vitamin B12 deficiency resulting from inadequate dietary intake is rare in children in the modern era because of improvements in nutritional status. However, such anemia can be caused by decreased ingestion or impaired absorption and/or utilization of vitamin B12. We report the case of an 18-year-old man with short stature, prepubertal sexual maturation, exertional dyspnea, and severe anemia with a hemoglobin level of 3.3 g/dL. He had a history of small bowel resection from 50 cm below the Treitz ligament to 5 cm above the ileocecal valve necessitated by midgut volvulus in the neonatal period. Laboratory tests showed deficiencies of both vitamin B12 and iron. A bone marrow examination revealed dyserythropoiesis and low levels of hemosiderin particles, and a cytogenetic study disclosed a normal karyotype. After treatment with parenteral vitamin B12 and elemental iron, both anemia and growth showed gradual improvement. This is a rare case that presented with short stature and delayed puberty caused by nutritional deficiency anemia in Korea.

Cloning and Sequencing of the ${\alpha}-1{\rightarrow}6$ Dextransurcrase Gene from Leuconostoc mensenteroides B-742CB

  • Kim, Ho-Sang;Kim, Do-Man;Ryu, Hwa-Ja;Robyt, John-F.
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.559-563
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    • 2000
  • A dextransucrase gene (dsrB742) that expresses a dextransucrase to synthesize mostly ${\alpha}-1{\rightarrow}6$ linked dextran with a low amount (3-5%) of ${\alpha}-1{\rightarrow}3$ branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1-kb PstI fragments were ligated with pGEM-3Zf(-) and transformed into E. coli $DH5{\alpha}$. The recombinant clone (pDSRB742) synthesized dextran on an agar plate containing 2% (w/v) sucrose. The dextran synthesized was hydrolyzed with Penicillium endo-dextranase. The hydrolyzate was composed of glucose, isomaltose, isomaltotriose, and branced pentasaccharide. The nucleotide sequence of dsrB742 showed one open reading frame (ORF) composed of 4,524 bp encoding dextrasnsucrase. The deduced amino acid sequence revealed a calculated molecular mass of 168.6 kDa. It also showed an activity band of 184 kKa on a non-denaturing SDS-PAGE (10%). The amino acid sequence of DSRB742 exhibited a 50% similarity with DSRA from L. mesenteroides B-1299, a 70% similarity with DSRS from L. mesenteroides B-512 (F, FMCM) and a 45-56% similarity with Streptococcal GTFs.

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A Study on the Emotional Evaluation Model of LED Colors in Architectural Pacade (건축파사드 LED칼라의 감성평가모형 연구)

  • Yoo, Yong-Woo
    • Korean Institute of Interior Design Journal
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    • v.21 no.2
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    • pp.101-111
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    • 2012
  • The purpose of this study is to draw emotional evaluation model related to LED lighting colors and suggest basic informations that enable planned directions of architectural pacade lighting to simulate beforehand in a laboratory. As experimental methods, extracted PAD emotional evaluation model and then directed a experimental model of 10 LED lighting colors, studied and analyzed survey questions. The results are as follows; First, in the case of P(Pleasure-Unpleasure), Red, Yellow, Green, Cyan, Magenta, White and Combination of $G{\leftrightarrow}B$ Colors are satisfying, vibrant and positive distribution chart has shown. Second, in the case of A(Arousal-Unarousal), Red, Yellow, Green, Cyan, Blue, White and Combinations of $R{\leftrightarrow}G$, $G{\leftrightarrow}B$, $R{\leftrightarrow}B$ Colors, it makes a little difference, they have shown useful distribution chart to induce sight in a aroused state. Third, in the case of D(Dominance-Submissiveness), Red, Cyan, Green and Combinations of $G{\leftrightarrow}B$ Colors are free, controlling and extent of effect was excellent. On the contrary, in the case of Yellow and Magenta and Combinations of $R{\leftrightarrow}B$, they are gentle and arouse the protective instinct or feminine distribution chart has shown. Fourth, it showed difference of preference that difference of select ratios of both men and women in Orange, Blue, Greenish-Yellow (preference of man) and Magenta, Combinations of $R{\leftrightarrow}B$ Colors(preference of woman).

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Adjuvant Effects of Iota-Carrageenan on the Immunoglobulin G Production (면역글로부린 G 생성에 대한 아이오타 - 카라기난의 어쥬번트 효과)

  • Koo, Bon-Woong;Lee, Kwang-Jae;Kim, Man-Suk;Kim, Ha-Hyung
    • YAKHAK HOEJI
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    • v.44 no.6
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    • pp.588-594
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    • 2000
  • To investigate the effects of iota-carrageenan (CAR) and/or alum on the adjuvancity as well as the structural difference of oligosaccharide on the IgG2b in the adjuvant effect, C57BL/6 mice were immunized twice with fetuin as a model antigen. CAR alone showed no significant effect on induction of antibody except IgG1. In contrast, Alum-CAR (after mixing of antigen-Alum, CAR adjuvant was prepared) and CAR-Alum (after formulation of antigen-CAR, Alum adjuvant was prepared) enhanced production of antibody, especially, IgG2b. After separation of IgG2b, changes of glycosylation were investigated using enzymelinked lectin assay. High affinity of IgG2b to N-acetylneuraminic acid, galactose and mannose-specific lectin were induced by CAR-Alum adjuvant, however, the affinity of IgG2b induced by CAR-Alum to GlcNAc and GalNAc-specific lectin were much less than that induced by Alum-CAR.

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Printed Reflectarray Antenna Design for Parabolic Reflector Volume Reduction (파라볼릭 반사기 체적 축소용 프린트 리플렉트어레이 안테나 설계)

  • Moon, Sang-Man;Kim, In-Kyu
    • Aerospace Engineering and Technology
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    • v.12 no.2
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    • pp.140-146
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    • 2013
  • In this study, we discuss about the printed reflectarray antenna design for parabolic reflector volume reduction. For this, we simulated and measured the phase characteristics of the unit array element of reflectarray antenna using waveguide simulator. As a results, the maximum phase variation is $298^{\circ}$ by simulation, the average phase variation is $309^{\circ}$ by measurement in 10GHz. And the printed Reflectarray antenna gain is 28.3dBi, 3dB beamwidth is E-plane $5.1^{\circ}$, H-plane $5.2^{\circ}$, sidelobe level is E-plane -11.4dB, H-plane -17.6dB.

Characteristics of Erythemal Ultraviolet Irradiance operating at Korea Meteorological Administration (기상청에서 운용 중인 지역별 지표 홍반자외선(EUV-B) 복사의 특성)

  • Hong Gi-Man;Choi Byoung-Cheol
    • Journal of Korean Society for Atmospheric Environment
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    • v.22 no.2
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    • pp.223-233
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    • 2006
  • We analyzed the monthly and seasonal mean of the daily Erythemal Ultraviolet-B (EUV-B, $280{\sim}320nm$) irradiance operating in Pohang, Anmyeon, Gosan, Mokpo and Kangnung with UV-Biometer (Solar Light Co., Model No. 501) at clear-sky noon during the period from 1999 to 2004. Also, we investigated the seasonal and regional characteristics for the UV index over the Korean Peninsula. The daily maximum occurred near solar southing time and the highest monthly accumulated EUV-B irradiance appeared in July and August at each regional observatory. The monthly mean value of the clear-sky EUV-B irradiance in Pohang, Anmyeon, Gosan, Mokpo and Kangnung showed 196.6, 161.8, 221.9, $171.5mWm^{-2}\;and\;179.7mWm^{-2}$ near noon in July respectively. The annual mean value of the daily accumulated EUV-B irradiance in Pohang, Anmyeon, Gosan, Mokpo and Kangnung were 1.8, 2.1, 2.2, $1.8kJm^{-2}\;and\;1.5kJm^{-2}$ respectively. The UV Index (UVI) showed above UVI 7(High) more than 90 days during one year over the Korean Peninsula.

A Study of Immunogenicity and Reactogenicity of Hepatitis B Vaccine Made by Recombinant DNA Techniques in Yeast (효모재조합 DNA B형 간염백신의 면역효과에 관한 연구)

  • Min, Chang-Hong;Kim, Kyo-Myung;Lee, Kyu-Man
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.2
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    • pp.243-249
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    • 1986
  • A study of the immunogenicity and reactogenicity of two doses of lot H(10, 20 mcg), two doses of lot L (20, 40 mcg) of the Smith Kline-RIT recombinant DNA yeast-derived hepatitis B vaccine and a 20-mcg dose of the Merck Sharp and Dohme plasma-derived hepatitis B vaccine was conducted in young adults under randomized, double-blind conditions. Immunization was carried out according to a 0-, 1-, and 6-month vaccination schedule. Results indicated that the yeast-derived hepatitis B vaccine was well tolerated and immunogenic. Reactogenicity to both yeast- and plasma-derived vaccines was mild in severity and low in incidence with no significant differences appearing between the study groups. One month after the third dose, the yeast-derived vaccines induced a high degree of soroconversion ranging between 95.0% and 100%. The response was not lot or dose-dependent. The administration of the plasma-derived vaccine resulted in anti-HBs geometric mean titres statistically signifirantly higher than those elicited by the different yeast-derived hepatitis B vaccines one month after the third dose of vaccine but the difference was not large enough to be of great clinical significance.

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A Study on the Crash Characteristics and Analysis of Spot+adhesive Welds in Automobile B-pillar Parts (자동차 B-pillar부품의 스폿용접 및 접착 혼용 용접부의 충돌특성 및 해석에 관한 연구)

  • Choi, Young-Soo;Yun, Sang-Man;Cho, Yong-Joon;Rhee, Se-Hun
    • Journal of Welding and Joining
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    • v.29 no.5
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    • pp.72-81
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    • 2011
  • In the present day, the needs of new steel for lightweight car-body have been increased in the automotive industry. however, the resistance spot welding is difficult to apply to the new steel because of the narrow weld current range and defects. As the solutions to these problems, adhesive bonding process is proposed. Adhesive bonding which reduce noise and vibration can be applied to joining the new steel. In this study, crash tests of b-pillar applied the resistance spot welding, structural adhesive bonding, the mixture of the structural adhesives and resistance spot welding were performed. And FEM crash model for b-pillar applied the structural adhesive bonding was developed. The results of experiment and analysis on b-pillar crash test were compared to verify the validity.