• 한국산림과학회지
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• 제102권4호
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• pp.530-536
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• 2013

본 연구는 산림환경이 인체 내 과산화지질 농도 변화에 영향을 줄 수 있는지 확인하고, 산림치유 효과 측정을 위한 생리적 지표로써 과산화지질의 활용가능성을 검토하고자 하였다. 피험자 29명을 대상으로 도시지역인 제주도의 제주시청 앞 인도와 산림지역인 삼나무 숲길에서 심리적 지표인 기분상태척도와 생리적 지표인 과산화지질수준 변화를 걷기 전후에 분석하였다. 도시지역에 비해 산림지역에서 기분상태척도의 총기분장애점수가 유의하게 낮아져 심리적으로 안정되는 효과를 보였다. 또한 과산화지질 수준은 도시지역에서 2.9에서 3.5 수준(5점 수준, 만점)으로 유의하게 높아졌고, 산림지역에서는 2.4에서 1.9 수준으로 낮아지는 경향을 보였는데, 위와 같은 결과는 과산화지질이 단기간 환경노출에 의한 인체에 미치는 영향을 파악하는데 적합한 지표라는 것을 보여준다. 산림과 도시에서 대기환경 및 심리적 안정도가 달라 과산화지질 수준의 변화 경향도 서로 다르게 나타난 것으로 보인다. 향후 연구에서는 장기간의 환경노출이 인체에 미치는 영향을 파악하기 위한 생리적 지표로써 과산화지질의 적합성을 분석하고, 새로운 생리적 지표를 지속적으로 발굴하는 것이 필요하다.

• 한국환경과학회지
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• 제1권2호
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• pp.81.1-86
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• 1992

The amounts of ascorbic acid in chloroplasts treated with light and light+paraquat (PQ) were reduced by 81 and 82% of initial level, respectively at 24 hr at incubation. And those treated with dark and dark+PQ were decreased by W and 55% of the original level, respectively. Malondialdehyde (MDA) contents at 24 hr of dark and dark+PQ treatment were increased by 6 and 31% of the initial level, respectively. When chloroplasts were treated with light and light+PQ, MDA contents after 24 hr were increased by 88 and 146% of the initial level, respectively. SOD activities treated with light and light+PQ were increased by 10 and 20% of the initial level, respectively for 3 hr and thereafter reduced by 46 and 49% of the original level, respectively at 24 hr. However, the SOD activities treated with dark and dark+PQ were decreased by 37 and 30% of the initial level, respectively. It is considered that PQ triggers the oxidation of ascorbic acid, the induction of lipid peroxidation and the inactivation of SOD under light so that PQ has inhibitors effect on the pathway of plant metabolism. Key word: ascorbic acid, malondialdehyde, superoxide dismutase, paraquat, lipid peroxidation.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제1권2호
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• pp.81-86
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• 1997

The amounts of ascorbic acid in chloroplasts treated with light and light+paraquat (PQ) were reduced by 81 and $82\%$ of initial level, respectively at 24 hr at incubation. And those treated with dark and dark+PQ were decreased by 46 and $55\%$ of the original level, respectively. Malondialdehyde (MDA) contents at 24 hr of dark and dark+PQ treatment were increased by 6 and $31\%$ of the initial level, respectively. When chloroplasts were treated with light and light+PQ, MDA contents after 24 hr were increased by 88 and $146\%$ of the initial level, respectively. SOD activities treated with light and light+PQ were increased by 10 and $20\%$ of the initial level, respectively for 3 hr and thereafter reduced by 46 and $49\%$ of the original level, respectively at 24 hr. However, the SOD activities treated with dark and dark+PQ were decreased by 37 and $30\%$ of the initial level, respectively, It is considered that PQ triggers the oxidation of ascorbic acid, the induction of lipid peroxidation and the inactivation of SOD under light so that PQ has inhibitory effect on the pathway of plant metabolism.

• Journal of Forest and Environmental Science
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• 제26권3호
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• pp.171-179
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• 2010

Effect of chromium (Cr) stress on antioxidant enzyme activities and malondialdehyde (MDA) content were investigated in leaves and roots of mangrove (italic (L.) Druce) seedlings. Cr toxicity effects were also assessed on young seedlings. The seedlings were grown in green house condition for three months in nutrient solution with 0, 0.5, 1, 1.5, 2, 2.5, and 3 mg $L^{-1}$ $CrCl_3$. This study showed that Cr led to the change of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and activities at different concentrations. The activity of antioxidant enzymes in leaves of K. candel seedlings indicates that enzymes engaged in antioxidant defense in certain level especially in low concentration of Cr treatments. The activities of SOD and POD were activated by Cr in the root level, while CAT activity was inhibited. CAT activity decreased in response to high concentrations of Cr. In the present study indicated that SOD in root was active in scavenging the superoxide produced by Cr. Both in roots and leaves, an increase in malondialdehyde (MDA) content was observed with increase in metal concentration and exposure periods. Our finding indicated that the high concentration of excessive Cr supply may interfere with several metabolic processes of seedlings, causing toxicity to plants as exhibited by chlorosis, necrosis, photosynthetic impairing and finally, plant death.

• Biomolecules & Therapeutics
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• 제2권3호
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• pp.298-302
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• 1994

Wistar albino rats were injected subcutaneously with mercuric chloride (5 mg/kg) to define the early biochemical determinants that participate in the pathogenesis of mercuric chloride-induced hepatotoxicity, especially focusing on oxygen free radicals, we studied malondialdehyde(MDA) level and the activities of catalase and superoxide dismutase in the liver of rats at 24, 48 and 72 hr after the injection of mercuric chloride. MDA levels at 24, 48 and 72 hr after the injection of mercuric chloride increased as compared with that of control group. The activities of catalase and superoxide dismutase at 24, 48 and 72hr after the injection of mercuric chloride decreased as compared with that of control group. These results suggest that the depression of the activities of catalase and superoxide dismutase resulting from excessive oxygen free radicals is an important determinant in pathogenesis of mercuric chloride-induced hepatotoxicity.

• 대한의생명과학회지
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• 제28권4호
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• pp.307-311
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• 2022

The extract of EA lacks studies showing its efficacy other than that it contains caffeic acid, an active compound that has antioxidant and neuroprotective effects on nerve cells. Therefore, in this study, we attempted to determine the effectiveness of EA extraction. In this study, we performed a DPPH assay to determine the antioxidant potential of EA. And then, the cytotoxic concentration of EA in HaCaT keratinocytes was determined, and the antioxidant effect was determined by measuring the malondialdehyde (MDA). The results of DPPH, a chemical antioxidant assay, clearly demonstrated the antioxidant capacity of EA extracted with distilled water. In addition, cell-based assays provide useful information on the protective effect of EA on oxidative stress-induced apoptosis.

• Journal of Ginseng Research
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• 제35권3호
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• pp.339-343
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• 2011

Polycyclic aromatic hydrocarbons (PAHs) are well known environmental carcinogens. PAH metabolites, especially BaP-7,8- dihydrodiol, 9,10 epoxide, initiate carcinogenesis via high specificity binding to DNA to form DNA adducts. The Korean red ginseng (KRG) from Panax ginseng has been suggested to protect against damages due to PAH exposure but the mechanism is unknown. Therefore, we investigated effects of KRG on PAH exposure using toxicokinetic methods and changes of PAH-induced oxidative damage during a 2 week-clinical trial (n=21 healthy young female, $23.71{\pm}2.43$ years). To analyze antioxidative effects of KRG, we measured changes in the levels of urinary malondialdehyde (MDA) before and after KRG treatment. We observed a significant positive association between levels of urinary MDA and 1-hydroxypyrene, a biomarker of PAH exposures (slope=1.47, p=0.03) and confirmed oxidative stress induced by PAH exposures. A reverse significant correlation between KRG treatment and level of urinary MDA was observed (p=0.03). In summary, results of our clinical trial study suggest that KRG plays a significant role in antioxidative as well as toxicokinetic pathways against PAHs exposure.

• 한국환경과학회지
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• 제1권2호
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• pp.87-91
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• 1992

Leaf discs were excised from spinach leaves (Spinaia oleracea L.) and floated in phosphate buffer (pH 6.8) containing paraquat solutions (0, 0.1, 1.0, 10, 20, 50 and 100 ppm), and incubated in the growth chamber under 5, 500 lux illumination at $25^{\circ}C$ for 24 hr. Treatment with paraquat caused the formation of malondialdehyde (MDA), an indicator of lipid peroxidation in leaf discs. When 1.0, 10, 20, 50 and 100 ppm of paraquat solutions were applied to leaf discs, the contents of MDA were increased by 63, n6, 100, 140 and 150% of the level without paraquat treatment, respectively. 1.0, 10, 20, 50 and 100 ppm of paraquat treatments reduced the amounts of ascorbic acid in leaf discs by 23, 35, 38, 42 and 56% of the level without paraquat treatment respectively. Activities of superoxide dismutase (SOD) in leaf discs of 1.0, 10, 20, 50 and 100 ppm of paraquat treatments were decreased by 23, 42, 48, 61 and 70% of the level of SOD in non-treated group, respectively. The results suggest that paraquat may cause peroxidation of membrane lipid in spinach leaves as a result of paraquat-induced destruction of physiological defense against oxygen phytotoxicity.

• Journal of Nutrition and Health
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• 제33권3호
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• pp.289-295
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• 2000

In the present work we investigated the effect of regular physical exercise on the activities of erythrocyte antioxidant enzyme, plasma total radical-trapping antioxidant potential(TRAP) and plasma level of lipid peroxidation(malondialdehyde, MDA) in 64 healthy male, aged 34-67 years. The study population were divided in two groups: small amount of exerciser(exercise time less than 10min/d) and moderate amount of exerciser(exercise time more than 20min/d) according to their physical exercise habits measured by a questionnaire. Erythrocyte superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and catalase(CAT), plasma TRAP, as well as plasma MDA were determined. Erythrocyte GSH-Px and plasma TRAP were higher in moderate amount of exercisers than those in small amount of exercisers by 17% and 26%, respectively. No significant differences were observed in erythrocyte SOD, CAT and plasma MDA between the two groups. Mean exercise time was positively correlated with the erythrocyte GSH-Px activity and plasma TRAP significantly. The results would sugest that regular moderate exercise enhances antioxidant defences against reactive oxygen species and may increase the likelihood of a healthier life span.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제1권2호
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• pp.87-91
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• 1997

Leaf discs were excised from spinach leaves (Spinacia oleracea L.) and floated in phosphate buffer (pH 6.8) containing paraquat solutions (0, 0.1, 1.0, 10, 20, 50 and 100 ppm), and incubated in the growth chamber under 5,500 lux illumination at $25^{\circ}C$ for 24 hr. Treatment with paraquat caused the formation of malondialdehyde (MDA), an indicator of lipid peroxidation in leaf discs. When 1.0, 10, 20, 50 and 100 ppm of paraquat solutions were applied to leaf discs, the contents of MDA were increased by 63, 86, 100, 140 and $150\%$ of the level without paraquat treatment respectively. 1.0, 10, 20, 50 and 100 ppm of paraquat treatments reduced the amounts of ascorbic acid in leaf discs by 23, 35, 38, 42 and $56\%$ of the level without paraquat treatment, respectively. Activities of superoxide dismutase (SOD) in leaf discs of 1.0, 10, 20, 50 and 100 ppm of paraquat treatments were decreased by 23, 42, 48, 61 and $70\%$ of the level of SOD in non-treated group, respectively. The results suggest that paraquat may cause peroxidation of membrane lipid in spinach leaves as a result of paraquat-induced destruction of physiological defense against oxygen phytotoxicity.