• BMB Reports
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• 제32권1호
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• pp.25-27
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• 1999

Otitis media with effusion (OME) is an inflammatory disease of the middle ear cleft. Oxygen free radicals have been implicated in a variety of inflammatory disorders. Oxygen free radicals may also be involved in the pathogenesis of OME. To evaluate the involvement of oxygen free radicals in the pathogenesis of OME, the level of malondialdehyde, which gives an index of lipid peroxidation by oxygen free radicals, was measured by the reaction with thiobarbituric acid. Malondialdehyde level in the middle ear fluid from the OME group was higher than that in the normal control group. Malondialdehyde level in the middle ear fluid from a mucoid subgroup was higher than that in the serous subgroup. Malondialdehyde levels in the middle ear fluid from the serous subgroup was significantly correlated with symptom duration. The Pearson correlation coefficient between malondialdehyde levels in the middle ear fluid from the serous subgroup and symptom duration was 0.842 (P<0.05). These results indicate that lipid peroxidation by oxygen free radicals may be involved in the pathogenesis of human OME.

• 대한한방부인과학회지
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• 제27권3호
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• pp.116-123
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• 2014

Objectives: This study aims to research correlation of the malondialdehyde level in urinalysis with cold hypersensitivity. Methods: We studied 128 patients visiting ${\bigcirc}{\bigcirc}$ Korean Hospital from March, 2013 to May, 2013. The subjects were categorized into two groups: cold hypersensitivity group (n=46) and control group (n=79). Patients in cold hypersensitivity group had been stressed because of severe cold hypersensitivity. First, We investigated the difference of the level of malondialdehyde between two groups. In addition, temperature of the two points, ST32 and LR3, was measured by using Digital Infrared Thermal Imaging (DITI) to find out whether the extent of cold hypersensitivity is related to the level of malondialdehyde. Results: There is no different characteristics between two groups. The average of malondialdehyde in cold hypersensitivity group ($1613.15{\pm}1260.71$) is significantly higher than in control group ($1170.01{\pm}1015.66$)(p=0.04). But there is no correlation between level of malondialdehyde and the extent of cold hypersensitivity (r=-0.22, p=0.13). Conclusions: Although the level of malondialdehyde in cold hypersensitivity group is higher than in control group, there is no significant correlation between level of malondialdehyde and the extent of cold hypersensitivity.

• Asian-Australasian Journal of Animal Sciences
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• 제25권8호
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• pp.1190-1196
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• 2012

This study was conducted to evaluate effects of storage temperatures ($4^{\circ}C$ and $20^{\circ}C$) and pig breeds (Laiwu pig and Large White pig) on the main antioxidative enzymes (superoxide dismutase, catalase, and glutathione peroxidase) activity and lipid oxidation in porcine Longissimus dorsi muscle. Activities of antioxidative enzymes (AOE) decreased slightly during storage, regardless of storage temperatures. Muscle antioxidative enzymes activities stored at $4^{\circ}C$ were higher than that stored at $20^{\circ}C$. Laiwu pig's enzymes activities were significantly (p<0.01) higher than Large White's. The level of malondialdehyde is a direct expression of the grade of lipid oxidation in meat. In our study, the malondialdehyde contents increased after 6 days storage. However, malondialdehyde contents of Laiwu pig were significantly (p<0.01) lower than Large White's. A lower content of malondialdehyde corresponds to a lower oxidation of lipids. These results indicated the muscle antioxidative ability of Laiwu pig was higher than Large White pig. It also implied that antioxidative enzymes were involved in the essentials and deciding mechanisms of meat quality by quenching oxygen free radicals and inhibiting lipid oxidation in muscle.

• Archives of Pharmacal Research
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• 제9권3호
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• pp.189-191
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• 1986

The effects of diterpene acids (i. e. pimaradienoic acid, kaurenoic acid, hydroxy cembratrienoic acid and dihydroxycembratetraenoic acid) on malondialdehyde generation by rat platelets in response to thrombin were studied. All the compounds inhibited the generation of MDA.

• Biomolecules & Therapeutics
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• 제7권2호
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• pp.121-126
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• 1999

In an attempt to define the effects of hyperbaric oxygen treatment on the lipid peroxidation and oxygen free radical reactions in rats exposed to carbon monoxide, we studied malondialdehyde (MDA) level and activities of catalase and superoxide dismutase in the kidney of the rats exposed to carbon monoxide. Male Sprague-Dawley albino rats weighing 240 to 260 gm were used. Experimental groups consist of Control group (=breathing with air), HBO group (=exposed to hyperbaric oxygen 〔HBO, 3ATA, 100%〕 after air breath), CO group (=exposed to CO〔3,970 ppm〕after air breath), CO-Air group (=exposed to CO after air breath followed by air breath) and CO-HBO group (=exposed to CO after air breath followed HBO treatment). The CO group showed significantly higher MDA level, catalase activity and SOD activity as compared to that of control group. The CO-HBO group showed significantly lower MDA level as compared to that of CO group, and did not show significantly lower catalase activity and SOD activity as compared to that of CO group. These results suggest that the excessive oxygen free radicals is an important determinant in pathogenesis of CO-induced nephrotoxicity and HBO inhibits the lipid peroxidation caused by excessive oxygen free radicals in the kidney of the rats exposed to carbon monoxide.

• Biomolecules & Therapeutics
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• 제5권4호
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• pp.331-335
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• 1997

In a preliminary screening of plant extracts for the antigastritic and antiulcer actions in rats, the methanol extracts of Taheebo showed positive activity in HCI . ethanol-induced gastric lesion. Among the systematic fractions of hexane, chloroform, butanol and water, the most potent $H_2O$ fraction reduced significantly HCI . ethanol-induced gastric lesion at the oral dose of 300 mg/kg. In pylorus ligated rats chloroform and butanol fraction showed decreases in the volume of gastric secretion and acid output of which effects were stronger in chloroform fraction. Further assays with hexane butanol and $H_2O$ fraction disclosed that it significantly suppressed the aspirin-induced ulcer. The butanol fraction reduced significantly acetic acid induced ulcer at the dose of 400 mg/kg. The butanol and $H_2O$ fraction reduced the malondialdehyde level in HCI . ethanol-induced gastric lesion. In pylorus ligated rats, chloroform and butanol fraction reduced the malondialdehyde level and in aspirin-induced ulcer, chloroform fraction reduced that levle. These results might suggest that the butanol and $H_2O$ fraction of Taheebo had inhibitory action in gastric lesion and ulceration through inhibition of gastric acid secretion and the decrease malondialdehyde level.

• 한국식품영양과학회지
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• 제27권2호
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• pp.344-349
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• 1998

The purpose of this study was carried out to investigate the effect of scoparone(6, 7-dimethoxyco-umarin) on liver function. Sprague-Dawley rats were treated with scoparone at a dose of 20mg/kg body weight for 5 days. Hepatic bile flow, liver weight, BSP(bromosulfophthalein) biliary excretion, alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activities, malondialdehyde production and lactate dehydrogenase(LDH) release were assayed. Among them, ALT and AST activities, malondialdehyde production and LDH release were assayed by using primary hepatocyte cultures at a concentration of 0.1mg/ml. Scoparone treatment had no effect on liver weight and hepatic bile flow. Scoparone treatment not only increased BSP biliary excretion, but also recovered the decreased BSP biliary excretion by CCl4, Also scoparone significantly decreased with the increases of ALT and AST activities, malondialdehyde production and LDH release induced by CCl4. These results suggested that scoparone could protect the liver damage by chemicals via promoting the liver excretory function.

• 한국환경농학회지
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• 제20권2호
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• pp.93-98
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• 2001

저온에 내성인 자포니카의 일품벼와 감수성인 인디카의 태백벼 품종간에 저온처리기간 동안 및 저온처리 후 회복동안에 나타날 수 있는 생리적 반응을 비교하였다. $5^{\circ}C$의 저온처리 3일 동안에 잎의 생체중 및 엽록소 함량은 두 품종간에 차이가 적거나 없었으나 저온처리 후 $25^{\circ}C$에 회복시킨 동안에는 태백벼에 비해 일품 벼가 더 빨리 회복되었다. 상대적 수분함량, 지질과산화 작용의 지표인 malondialdehyde 생성량 및 엽록소 형광은 저온처리기간 동안 및 저온처리 후 회복기간동안에도 두 품종간에 유의적인 차이를 보였고, 이들 생리적 지표들은 생체중 및 엽록소 함량보다는 더 민감한 생리적 지표이었다. 두 품종간에 상대적 수분함량, malondialdehyde 생성량 및 엽록소 형광의 차이는 저온처리 후 회복기간동안보다는 저온처리동안에서 더 적었다. 따라서 이들 결과를 통해서 볼 때 상대적 수분함량, malondialdehyde 생성량과 엽록소 형광은 저온에 내성을 선발하는데 중요한 생리적 지표로 이용할 수 있으리라 생각한다.

• Journal of Nutrition and Health
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• 제35권1호
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• pp.24-29
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• 2002

본 연구에서는 녹차 건분 식이가 뇌 조직에서 알코올에 의한 산화적 스트레스 감소효과를 나타내는 지를 규명하고 자, 생후 9개월령 Sprague-Dawley에게 1% 녹차 건분식이를 4주간 공급하고 희생 12시간 전메 급성으로 알코올을 투여한 후 뇌 조직을 cortex, cerebellum, striatum, hippocampus로 나누어 malondialdehyde(MDA)의 함량과 catalase. superoxide dismutase(SOD), glutathione peroxidase(GSH-Px)의 활성을 측정하였다. 본 실험 결과를 종합하여 볼 때. 1%녹차 건분 식이의 공급은 striatum과 hippocampus 내 MDA 함량을 유의적으로 낮추고 알코올에 따른 hippocampus 내 catalase 활성 증가를 유의적으로 억제하였다. 이러한 곁과는 녹차 건분의 공급이 알코올로 산화적 stress를 가한 동물에서 일부 뇌 조직의 지질 산화를 감소시키고 알코올에 의한 뇌 조직 손상으로부터 보호할 수 있음을 보여준다.

• Parasites, Hosts and Diseases
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• 제50권4호
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• pp.375-377
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• 2012

Malaria is still a leading cause of morbidity and mortality. The increase in lipid peroxidation reported in malaria infection and antioxidant status may be a useful marker of oxidative stress during malaria infection. The aim of this study was to investigate the role of antioxidant enzymes against toxic reactive oxygen species in patients infected with Plasmodium vivax and healthy controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were determined in 91 P. vivax patients and compared with 52 controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were $8.07{\pm}2.29$ nM/ml, $2.69{\pm}0.33$ U/ml, and $49.6{\pm}3.2$ U/g Hb in the patient group and $2.72{\pm}0.50$ nM/ml, $3.71{\pm}0.47$ U/ml, and $62.3{\pm}4.3$ U/g Hb in the control group, respectively. Malondialdehyde levels were found statistically significant in patients with vivax malaria higher than in healthy controls (P<0.001). On the other hand, superoxide dismutase and glutathione peroxidase activities were found to be significantly lower in vivax malaria patients than in controls (P<0.05). There was an increase in oxidative stress in vivax malaria. The results suggested that antioxidant defense mechanisms may play an important role in the pathogenesis of P. vivax.