• Title/Summary/Keyword: male sterile

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Breeding for Potato Virus Y Resistant Male-sterile $F_1$ Hybrid KB 109 in Nicotiana tabacum L. (연초의 감자바이러스 Y 저항성 웅성불임 일대잡종 KB 109 육성)

  • 조천준;김대송;정석훈;최상주;조명조
    • Journal of the Korean Society of Tobacco Science
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    • v.16 no.2
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    • pp.134-138
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    • 1994
  • Potato Virus Y(PVY), vein necrosis strain, in Korea causes severe symptoms on burley tobacco(Nicotiana tabacum L.). As the results, programs to incorporate PVY resistance into commercial cultivars were initiatEd. But the development of the homozygous fertile line resistant to PVY is time consumming. This study was conducted whether the Fl hybrid could be used to reduce the yield losses caused by PVY. Four F1 hybrids were made between male - sterile(ms) NC 107 and KB 107 as maternal parent, and TC 612 and TC 613 as Pollen donor, respectively, and were evaluated for their PVY resistance and negatively associated traits. (ms NC 107 X TC 612) F1, named as KB 109, Ivas applied to yield trial and compared with commercial cultivars for the level of disease resistance, agronomic characteristics, chemical contents and physical properties. All Fl hybrid could be used commercially as the PVY resistant cultivar. Especially KB 109 have the resistance against PVY, tobacco mosaic virus and black shank(Phytophthora parasitica var. nicotianae). It had wider leaves, flowered one day later, and yield of acceptable quality was higher than that of Burley 21, standard cultivar in Korea.

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Studies on the Rice Stem Borer Control Using Sterile-Male Technique 1. On the Radiosensitivity of Rice Stem Bore. (Chilo suppressalis Walker) (웅성불임을 이용한 이화명충 방제에 관한 연구 1. 이화명충의 방사선 감수성에 관한 연구)

  • Chung K. H.;Ryu J.
    • Korean journal of applied entomology
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    • v.10 no.2
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    • pp.117-120
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    • 1971
  • This experiment was carried out to obtain basic informations on the development in sterile-male technique of rice stem borer. The species considered is Chile suppressalis Walker. Different developmental stages of the insect namely; larval, pupae and adult were irradiated with $Co^{60}$ gamma ray. The results obtained were as follows: 1) A 1:1 sex ratio in the pupal stage was observed. 2) In the pupal stage sub-lethal dosages$(LD_{50})$ were 26 KR and 26.7 KR for female and male, respectively. 3) The total number of egg masses per female increased with the increasing gamma ray dosages and average number of eggs produced per female decreased in the cross between normal female and irradiated male. 4) The sterile dosage $(SD_{90})$ of the gamma ray irradiation showed 23 KR in the male. 5) The pupal stage is most convenient developmental stage for irradiation.

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Sterile Necrosis of the Sternum: A Rare Complication Following Coronary Artery Bypass Surgery

  • Papadakis, Emmanouel;Konstantinidou, Maria Kalliopi;Kanakis, Meletios A.
    • Journal of Chest Surgery
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    • v.50 no.6
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    • pp.460-462
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    • 2017
  • We herein present the unique case of a 68-year-old male diabetic patient who developed sterile necrosis of the sternum 1 month after myocardial revascularization with the use of bilateral internal thoracic artery grafts. The sternum had been closed by the bilateral Robicsek wiring technique. The sternum was removed, and bilateral pectoralis major flaps were used to cover the defect. The patient had an uneventful recovery.

Utilization of Male Sterility Type and Frequency of Male Sterile Genes in Carrots (당근 웅성부임성(雄性不稔性)의 형태별(形態別) 이용현황(利用現況)과 핵내(核內) 웅성부임(雄性不稔) 유전자(遺傳子)의 분포(分布))

  • Suh, Young Ki;Youn, Goang Hyeon;Cho, Young Hwan;Paek, Kee Yoeop
    • Horticultural Science & Technology
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    • v.17 no.4
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    • pp.470-472
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    • 1999
  • When the type and ratio of male-sterile plants in $F_1$ hybrids collected from several seed companies were investigated, there were differences in the male-sterile type depending upon region, seed company and variety group. The differences were inferred due to the easiness in breeding of maintainer line among the variety groups. American seed companies mainly used petaloid type male sterility with Imperater group varieties. European companies used brown anther type male sterility mainly with Nantes group but some companies used petaloid type also in varieties that were different from Nantes group. Asian companies (Japan, Korea) used both types with Chantaney and Kuroda group varieties, but one type was mainly used depending on individual seed company. Only one type of male-sterility in one variety was observed and the results were agreed well with other's results that male-sterility type was determined by cytoplasm factor. Some breeding lines were backcrossed to both types of cytoplasm (Sa, Sp) for maintainer line selection. We could select 15 maintainer lines from 20 lines in petaloid cytoplasm (Sp) and 3 from 4 lines in brown cytoplasm (Sa). In petaloid cytoplasm, maintainer lines can be selected at considerably high frequency. But in brown anther cytoplasm, the used materials are too restricted to tell general frequency maintainer lines.

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Studies on Breeding of Hybrid Rice Using the Korean Cytoplasmic and Genetic Male Sterile Rice III. Yield and Grain Quality of the $F_1$ Rice Hybrids Developed by Using the Genetic Male Sterile Lines Having the Backgrounds of Korean Variety (세포질적 유전자적 웅성불임을 이용한 벼 일대잡종 육성방안 III. 유전자적 웅성불임계통을 이용한 일대잡종 벼의 수량과 미질)

  • Suh, Hak-Soo;Cho, Young-Chan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.2
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    • pp.151-156
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    • 1987
  • Eleven F$_1$ rice hybrids were developed by using the genetic male sterile lines having the backgrounds of Korean variety. The average heterosis, heterobeltiosis and standard heterosis for grain yield were 15.2%, 8.7% and 17.9%, respectively. Significant heterobe1tiosis for number of grains per panicle was observed. The leaf blast reaction of the hybrids was mostly similar to that of the more resistant parent. Amylose content of the hybrids ranged from 20.5% to 22.5% and protein content from 10.4% to 11.1%. The degrees of alkali digestion value, white center or white belly, clearance and heading date of the hybrids were almost same as their parents.

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Studies on the Heterosis Breeding in Rape by Using Cytoplasmic Genetic Male Sterile lines 3. Development of Complete Cytoplasmic Genetic Male Sterile Line "MOKPO-MS" Having Improved Quality of Oil and Oil Cake (세포질.유전자적 웅성불임 계통을 이용한 유채 Heterosis 육종에 관한 연구 제3보 유채 세포질 유전자적 웅성불임 계통의 불임안전성과 유.박의 성분 개량)

  • Lee, J.I.;Kwon, B.S.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.25 no.3
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    • pp.50-58
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    • 1980
  • A cytoplasmic-genetic male sterile line(MS) in rapes of which fertility is not restored under any temperature regimes and have the better quaility of and oil cake was developed. A MS line named Mokpo-MS, which is form the cross between Tower and Isuzu and its stamens is degenerated completely by interact~g with cytoplasmic and nuclear genes was selected. This MS line was found as a complete cytoplasmic-genetic male sterile having the quality of oil and oil cake were greatly improved by introducing Zero-erucic and Zero-glucosinolate gene from Tower.

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Inheritance Study of Male Sterile Transformants Containing Pollen-specific Promoter and Diphtheria Toxin A Gene (수술특이프로모터와 디프테리아 독성 유전자에 의한 웅성불임 형질전환체의 후대 유전분석)

  • Park, Young-Doo;Kim, Hyun-Uk;Park, Beom-Seok;Jin, Yong-Moon
    • Horticultural Science & Technology
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    • v.18 no.3
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    • pp.342-347
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    • 2000
  • The objectives of this study were to investigate the genetic and phenotypic features of male sterile transformants by pollen-specific expression of diphtheria toxin gene and to find out inheritance patterns of transgene to the next generation. When backcrossed (BC) progenies were tested for expression of kanamycin resistance ($Km^R$), 9 lines out of 13 lines, except 4 lines ($BC_{1}5-13,\;BC_{1}5-23,\;BC_{1}5-28,\;BC_{1}5-32$), showed the ratio of $Km^R$ to kanamycin sensitive ($Km^S$), from 1:30 to all $Km^S$. As a result, they were much lower than Mendelian segregation of a dominant gene. To determine whether male sterility is a heritable and stable trait, 5 male sterile plants ($BC_{1}5-13,\;BC_{1}5-14,\;BC_{1}5-23,\;BC_{1}5-32,\;BC_{1}5-33$ lines) which had different transgene copy numbers were backcrossed as female parents with pollens from wild type. To confirm the existence of the DTx-A gene in the genome of the progenies, PCR was conducted using specific primers of the DTx-A coding region. A PCR band of 428 bp was obtained from each generation, which is the predicted size of the DTx-A gene fragment. Trangenes were inherited to the next $BC_4T_0$ progenies and showed male sterility, however, based on the copy numbers of DTx-A gene male sterile plants did not show predicted ratio. When male sterile plants were backcrossed with fertile plants, fruit capsule sizes and seed settings were relatively reduced from those of selfing wild type plants. The fruit sizes and seed settings were reduced in proportion to the increase in the copy number of DTx-A gene.

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