• 대한화장품학회지
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• 제23권3호
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• pp.82-85
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• 1997

To develop an active material for skin darkening, we examined the effect of 300 plants on tyrosinase activity and found only Caesalpinia sappan has an ability to increase tyrosinase activity highly and melanin contents in B-16 melanoma cells. A compound increasing tyrosinase activity and melanin production was isolated from Caesalpinia sappan Lignum. Brazilin was identified as a new active agent. Brazilin increases the tyrosinase activity and malanin production of B-16 melanoma cells. In conclusion, it seems that brazilin cal be used as a new sunless tanning agent.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.157-167
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• 1987

The have been many reports that phenoloxidase are correlated with development in many fungi. C. congregatus, one of nushroom-forming basidiomycetes, which requires light for its development also has phenoloxidases. In C. congragatus, there are two sets of membrane-associated phenoloxidase (PHO I and PHO II) which are differentiated by their isozyme patterns, and each enzyme set consists of two different subtrate specific enzyme protein; o-tolidine reacting enzyme, and DOPA reacting enzyme. PHO I which is localized by a protoplast-concanavalin A technique by using a new solidifying agent, Pluronic Polyol F 127, instead of agar appears in the vegetative hyphae, and PHO II appears at the early primordial stage on agar and at the sclerotial stage of liquid shake cultures. Inhibition of PHO I with the enzyme inhibitors inhibits mushroom formation as well as melanization of the vegetative hyphae at concentrations which do not inhibit the vegetative growth. PHO I deficient mutants do not form mushrooms or melanins, and the mutants show abnormal nuclear migration patterns. PHO II has roles; possibly cementing the adjacent hyphae during the actual three dimensonal structure formation, and melanizing mushrooms and sclerotia. The possible roles of PHO I in the light reception complex and in melanin formation, the function of malanin, and possible roles of postulated post translational modifying enzymes which regulate the phenoloxidases, nuclear migration pattern, and self-nonself recognition mechanism are discussed.

• 대한화장품학회지
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• 제33권2호
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• pp.69-77
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• 2007

본 연구에서는 제주도 자생 감귤 17종에 대하여 탈색소효과(depigment effect)를 연구하였다. B16 멜라닌 생성세포(B16 melanoma cell)를 이용하여 tyrosinase 및 멜라닌 생성억제 효과를 연구한 결과 대조군 대비하여 농도 의존적으로 감소하는 3종의 미성숙 온주밀감(immature Citrus unshue), 팔삭(Citrus hassaku), 진지향(Citrus sinensis ${\times}$ reticulata)을 도출하였다. 또한, 이와 같이 도출된 감귤 추출물을 이용하여 멜라닌 세포 생성(melanogenesis)에 영향을 미치는 tyrosinase, DOPAchrome tautomerase (TRP-2)와 DHICA oxidase (TRP-1) 단백질 발현을 실험한 결과 tyrosinase, TRP-1, TRP-2 단백질 발현이 감소함을 나타내었다. 특히 이들 중 미성숙 온주밀감은 tyrosinase와 TRP-1 단백질 발현에서 유의적으로 감소하는 결과를 보였다. 본 연구 결과로써 미성숙 온주밀감은 모든 실험에서 우수한 효과를 나타내어 UV에 의해서 야기되는 피부 색소 침착을 방지할 수 있는 물질로서 기대할 수 있다.