• Journal of Nutrition and Health
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• 제54권4호
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• pp.335-341
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• 2021

Purpose: Muscle mitochondria play a key role in regulating fatty acid and glucose metabolism. Dysfunction of muscle mitochondria is associated with metabolic diseases such as obesity and type 2 diabetes. Isorhamnetin (ISOR), also known as 3-O-methylquercetin, a quercetin metabolite, is a naturally occurring flavonoid in many plants. This study evaluated the effects of ISOR on the regulation of the mitochondrial function of C2C12 muscle cells. Methods: C2C12 muscle cells were differentiated for 5 days, and then treated in various concentrations of ISOR. Cytotoxicity was determined by assessing cell viability using the water-soluble tetrazolium salt-8 assay principle at different concentrations of ISOR and time points. Levels of the mitochondrial DNA (mtDNA) content and gene expression were measured by quantitative real-time polymerase chain reaction. The citrate synthase (CS) activity was quantified by the enzymatic method. Results: ISOR at a concentration of 10 µM did not show any cytotoxic effects. ISOR increased the mtDNA copy number in a time- or dose-dependent manner. The messenger RNA levels of genes involved in mitochondrial function, such as peroxisome proliferator-activated receptor-γ coactivator-1α, and uncoupling protein 3 were significantly stimulated by the ISOR treatment. The CS activity was also significantly increased in a time- or dose-dependent manner. Conclusion: These results suggest that ISOR enhances the regulation of mitochondrial function, which was at least partially mediated via the stimulation of the mtDNA replication, mitochondrial gene expression, and CS activity in C2C12 muscle cells. Therefore, ISOR may be useful as a potential food ingredient to prevent metabolic diseases-associated muscle mitochondrial dysfunction.

• Human Ecology Research
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• 제61권1호
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• pp.123-139
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• 2023

The study examined the mediating effect of internalized shame on the relationship between affiliate stigma and interpersonal anxiety among adolescents with siblings who had autism spectrum disorder (ASD) and the moderated mediating effect of mother-adolescent communication openness. The participants consisted of 139 adolescents (boys 48.9%, high-school students 79.8%) who had siblings with ASD. Interpersonal anxiety, affiliate stigma, internalized shame, and mother-adolescent communication openness were measured using the Social Anxiety Scale for Adolescents (La Greca & Lopez, 1998), the Affiliate Stigma Scale (Mak & Cheung, 2008), the Internalized Shame Scale (Cook, 1988), and the Parent-Adolescent Communication Scale (Barnes & Olson, 1982), respectively. The data were analyzed using descriptive statistics and Pearson's correlation coefficients. Process Macro Models 4 and 7 were used to examine the mediating effect and the moderated mediating effect. The results indicated that internalized shame mediated the effect of affiliate stigma on interpersonal anxiety among adolescents who had siblings with ASD. However, there was no significant moderated mediating effect of mother-adolescent communication openness on the relationship between affiliate stigma, internalized shame and interpersonal anxiety. These findings suggest that it is necessary to improve social awareness of individuals with ASD and their family members to prevent adolescents who have siblings with ASD from having affiliate stigma and to help them reduce interpersonal anxiety. The results also highlight the importance of counseling programs for adolescents with siblings with ASD as a way of preventing or alleviating their interpersonal anxiety by reducing internalized shame, even where they experience affiliate stigma.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.77-77
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• 2020

Purple loosestrife-Lythrum anceps (Koehne) Makino is a herbaceous perennial plant belonging to the Lythraceae family. It has been used for centuries in Korea and other Asian traditional medicine. It has been showed pharmacological effects, including anti-oxidant and anti-microbial effects. However, the mechanisms underlying its anti-cancer mechanisms are not yet understood. In this study, we investigated the mechanism of apoptosis signaling pathways by ethanol extract of Lythrum anceps (Koehne) Makino (ELM) in human leukemia U937 cells. Treatment with ELM significantly inhibited cell growth in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies (ApoBDs), DNA fragmentation and increased populations of sub-G1 ratio. Induction of apoptosis by ELM was connected with up-regulation of death receptor (DR) 4 and DR5, pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic Bcl-2 protein, and inhibitor of apoptosis protein (IAP) family proteins (XIAP, cIAP-1, survivin), depending on dosage. This induction was associated with Bid truncation, mitochondrial dysfunction, proteolytic activation of caspases (-3, -8 and -9) and cleavage of poly(ADP-ribose) polymerase protein. Therefore, our data indicate that ELM suppresses U937 cell growth by activating the intrinsic and extrinsic apoptosis pathways, and thus may have applications as a potential source for an anti-leukemic chemotherapeutic agent.

• Korean Journal of Radiology
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• 제22권7호
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• pp.1132-1141
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• 2021

Objective: To investigate changes in the median nerve, retinaculum, and carpal tunnel on ultrasound after successful endoscopic carpal tunnel release (ECTR). Materials and Methods: This prospective study involved 37 wrists in 35 patients (5 male, 30 female; mean age ± standard deviation [SD], 56.9 ± 6.7 years) with primary carpal tunnel syndrome (CTS). An in-house developed scoring system (0-3) was used to gauge the clinical improvement after ECTR. Ultrasound was performed before ECTR, and at 1, 3, and 12 months post-ECTR. Changes in the median nerve, flexor retinaculum, and carpal tunnel morphology on ultrasound after ECTR were analyzed. Ultrasound parameters for different clinical improvement groups were compared. Results: All patients improved clinically after ECTR. The average clinical improvement score ± SD at 12 months post-ECTR was 2.2 ± 0.7. The median nerve cross-sectional area proximal and distal to the tunnel decreased at all time intervals post-ECTR but remained swollen compared to normal values. Serial changes in the median nerve caliber and retinacular bowing after ECTR were more pronounced at the tunnel outlet than at the tunnel inlet. The flexor retinaculum had reformed in 25 (68%) of 37 wrists after 12 months. Conclusion: Postoperative changes in median nerve and retinaculum parameters were most pronounced at the tunnel outlet. Even in patients with clinical improvement after ECTR, nearly all ultrasound parameters remain abnormal at one year post-ECTR. These ultrasound parameters should not necessarily be relied upon to diagnose persistent CTS after ECTR.

• 생물환경조절학회지
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• 제14권2호
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• pp.89-94
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• 2005

참외 무가온 재배 시 보온부직포 무게별 참외의 생육, 품질 및 수량에 미치는 영향을 검토하기 위하여 신토좌 대목에 금싸라기은천을 접목하여 정식 전부터 6온스, 12온스, 15온스 처리구와 대조구인 9온스를 4월 20일까지 덮어서 재배한 결과는 다음과 같다. 보온력이 높을수록 터널내 야간 기온이 높았는데, 6온스 $4.8^{\circ}C$, 9온스 $6.0^{\circ}C$, 12온스 $7.9^{\circ}C$ 그리고 15온스는 $8.0^{\circ}C$로 12온스와 15온스간에는 큰 차이는 없었다. 보온력이 높을수록 초장, 경경, 엽수, 엽면적, 생체중 및 건물중 등 정식 30일 후의 초기생육이 빨랐는데 특히 엽면적은 대조구인 9온스의 $370cm^2$ 비하여 12온스에서는 $116\%$, 15온스에서는 $129\%$였다. 정식 30일 후 일비액량은 9온스의 10.1mg에 비하여 12온스는 1.2배, 15온스는 1.9배 많았다. 9온스에서는 정식 47일 후 암꽃이 개화되었으나 15온스 및 12온스에서는 6일 빨랐으나 6온스에서는 3일 늦었고 첫 수확은 12 및 15온스에서 각각 3일, 4일 단축되었으나 6온스에서는 3일 늦었다. 평균과중, 과장, 과폭, 과육두께 및 당도는 15온스에서 가장 좋았고 12온스, 9온스, 6온스의 순이었다. 10a당 총수량은 15온스에서 가장 많았고 12온스, 9온스, 6온스의 순이었고, 발효과는 6온스에서 가장 많았고 9온스, 12온스, 15온스의 순이었고, 상품과 율은 15온스에서 가장 많았고 12온스, 9온스, 6온스의 순이었다.

• Applied Biological Chemistry
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• 제21권2호
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• pp.84-102
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• 1978

대맥(大麥) 무배아반편종자(無胚芽半片種子)에 $10{\mu}M$ GA를 처리(處理)하여 배양(培養) 10시간(時間)에 배지(培地)에서 ABA의 농도(濃度)가 각각(各各) $0.1{\mu}M,\;5{\mu}M$ 및 $10{\mu}M$이 되도록 ABA 용액(溶液)을 첨가하여 ${\alpha}-amylase$ 생성(生成)의 소장(消長)을 그리고 $10{\mu}M$ GA와 $10{\mu}M$ ABA 혼합액(混合液) 처리(處理)하여 10시간(時間) 배양(培養) 후(後) 핵산(核酸)의 거동을 $10{\mu}M$ GA처리구(處理區)의 그것과 비교(比較)하였다. 대맥(大麥) 초엽(？葉)에 $10{\mu}M$ GA와 $10{\mu}M$ ABA를 처리(處理)하여 초엽의 생육(生育), chlorophyll, RNase의 활성(活性), 단백질(蛋白質) 및 총(繼) RNA 함량(含量)의 소장(消長)을 처리구(處理區)의 그들과 비교(比較)하였고 이들 hormone 처리(處理) 20시간(時間) 배양후(培養後) 핵산(核酸)의 거동 및 polysome과 menosome의 상대적(相對的) 분포(分布)를 조사(調査)하여 몇 가지 결론(結論)을 얻었으며 그 결과(結果)를 요약(要約)하면 다음과 같다. 1) GA 처리(處理)에 의(依)한 총(總) ${\alpha}-amylase$의 생성(生成)은 시간(時間)의 경과(經過)에 따라 직선적(直線的)으로 증가(增加)하였으며 ${\alpha}-amylase$의 분비(分泌)는 배양(培養) 18시간(時間)부터 활발(活潑)하였다. 2) $0.1{\mu}M$ ABA 첨가는 ${\alpha}-amylase$의 생성(生成)을 부분적(部分的)으로 저해(沮害)시켰으나 $5{\mu}M$ 및 $10{\mu}M$ ABA 첨가는 다같이 첨가 4시간(時間) 후(後)에 완전(完全)히 ${\alpha}-amylase$의 생성(生成)을 저해(沮害)시켰으며, 배양과정중(培養過程中) $5{\mu}M$ ABA 첨가는 GA의 농도(濃度)에 관계(關係)없이 완전(完全)히 ${\alpha}-amylase$의 생성(生成)을 저해(沮害)시켰다. 3) ABA는 무배아반편종자(無胚芽半片種子)에서 ${\alpha}-amylase$의 분비(分泌)에 별(別) 영향(影響)을 주지 않았다. 4) 무배아반편종자(無胚芽半片種子)에서 GA 단독(單獨) 처리구(處理區)와 GA-ABA 처리구간(處理區間)에 핵산(核酸)의 분획(分劃)에서 별(別) 이(異)가 없었다. 5) 초엽에서 GA의 처리(處理)는 r-RNA 획분(劃分)을 증가(增加)시킨 반면(反面) ABA 처리(處理)는 r-RNA 획분(劃分)을 감소(減少)시킴과 동시(同時)에 s-RNA 획분(劃分)을 증가(增加)시켰는권(權) 이는 이들 hormone이 RNase의 활성(活性)에 상이(相異)한 영향(影響)을 준 것으로 보였다. 6) 초엽에서 ABA 처리(處理)는 RNA-DNA 획분(劃分)의 성분비(成分比)를 감소(減少)시켰다. 7) 초엽에서 GA 처리(處理)는 RNase의 활성(活性)을 감소(減少)시켰으나 ABA 처리(處理)는 이의 활성(活性)을 증대(增大)시켰다. 8) 초엽에서 GA 처리(處理)는 총(總) RNA에 큰 영향(影響)을 주지 않았으나 ABA 처리(處理)는 이를 현저히 감소(減少)시켰다. 9) 초엽에 있어서 GA 처리(處理)는 초엽의 생장(生長) 및 chlorophyll 함량(含量)을 증가(增加)시켰으나 ABA 처리(處理)는 이들을 감소(減少)시켰다. 10) 초엽에서 GA 처리(處理)는 단백질(蛋白質) 및 polysome의 형성(形成)을 촉진(促進)시켰으나 ABA 처리(處理)는 이들을 감소(減少)시켰다. 11) ABA 처리(處理)는 polysome의 형성(形成)이 저해(沮害)되는 까닭은 ABA가 r-RNA의 합성(合成)을 저해(沮害)하는 것으로 보였다.

• 공연문화연구
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• 제20호
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• pp.5-30
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• 2010

허규는 80~90년대 창극 연출을 담당하며 왕성한 활동을 전개한 바 있다. 그는 전승 5가 뿐만 아니라 실전 판소리를 창극화 하였으며, 창작 창극 작품도 다수 무대에 선보였다. 특히 '완판 창극'이라는 이름으로 1982년 <흥보전>을 무대에 올린 후 1985년 <적벽가>를 공연함으로써 전승 5가의 창극화 작업을 완결지은 것은 그가 남긴 큰 업적 가운데 하나이다. 허규는 주체적 민족문화의 정립과 한국 전통연희의 창조적 계승 문제에 많은 관심을 기울였던 실천적인 연극인이다. 그는 창극을 한국의 대표적인 공연예술로 정립하고자 노력했다. 창극 작품은 그 연원에 따라, (1)전승 5가의 창극화 (2)실전 7가의 창극화 (3)창작 창극으로 세분해 볼 수 있다. 허규가 연출한 작품에는 이 3가지 유형이 모두 포함되어 있다. 그 가운데 전승 5가를 창극화한 작품이 가장 큰 비중을 차지하고 있다. 허규가 시도한 '완판창극'은 한국의 전통유산 가운데 빼어난 예술적 성과를 거둔 요소들을 집대성하여 무대에 올림으로써, 창극을 한국의 대표적인 공연예술로 정립해 보고자한 것이다. '완판창극'에 나타난 특징은 다음 네 가지로 정리할 수 있다. (1) 전통을 중시하는 연출 태도, (2) 전통연희 요소의 적극적 수용, (3) 격조와 윤리의식의 중시, (4) 해학의 강조와 보조인물의 적극적 활용이 그것이다. 허규가 시도한 '완판창극'은 창극이 성취할 수 있는 예술적 수준의 한 정점을 보여주는 것이다. 판소리 유산을 망라하고 나아가 전통연희를 적극적으로 수용하여, 창극을 한국의 대표적인 공연예술로 정립해 보고자 했던 것이다. 허규는 '완판창극'에서 판소리의 진정성을 그대로 살리려고 노력했으며, 처음부터 끝까지 한 대목도 빠뜨리지 않고 장면화 하려고 했다. '완판창극'의 공연 시간이 4~5시간이나 소요되었다는 것이 그 점을 잘 보여준다. 허규가 완판창극에서 거둔 성과는 이후 창극에 상당한 영향을 끼친 것으로 보인다. 1990년대에 시도된 '완판장막창극'도 그 모태는 허규의 '완판창극'에 두고 있다. 창본을 종합해 내고 판소리의 좋은 점을 모두 보여주고자 하는 의도가 일치한다는 점에서 특히 그러하다. 그렇지만 90년대 '완판장막창극'은 대형 무대화를 지향했으며 화려한 무대장치와 의상 그리고 버라이어티한 요소를 부각시켰다는 점에서 '완판창극'과 대비된다. 허규의 완판창극이 끼친 중요한 영향 가운데 하나는 판소리의 '열린 형식'을 창극의 공연 문법으로 적극 활용했다는 점이다. 허규는 극의 전개 과정에서 필요하다고 판단되면 전통연희의 요소를 적극적으로 수용하여, 극적 표현 영역을 확장하고 작품의 완성도를 높이고자 했다. 이러한 그의 시도는 창극 극작술의 한 방식으로 인식되어, 이후 창극연출에도 지속적으로 영향을 미치고 있다. 요즘 창극은 어떻게 하면 청중들의 호응을 얻을 수 있을까에 관심을 집중하기 때문에, '감동받는 창극' 보다는 '재미있는 창극'을 만드는 일이 중요하다고 생각하는 듯하다. 공연 시간도 최대 2시간을 넘지 않으려고 하며, 관현악 반주를 중시하는 경향을 보여준다. 이런 관점에서는 허규가 '완판창극'을 통해 구현하고자 했던 창극의 지향점은 극복의 대상으로 인식되고 있는 것으로 보인다.

• 한국산림과학회지
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• 제48권1호
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• pp.59-63
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• 1980

삽목후(揷木後) 발근(発根)하기 까지에 있어 발근용역(発根容易)한 수종(樹種)과 곤란(困難)한 수종(樹種)간의 통수저항(通水抵抗)이 어떻게 변화(变化)하고 있는가를 삽수(揷穗)의 부의별(部位別)로 측정(測定)하였다. 1. 삽수(揷穗)의 전체(全体) 저항(抵抗)(RT)은 수종(樹種)에 의해 크게 달랐으며 발근용역(発根容易)한 수종(樹種) (Euonymus japonica. Viburnum Awabuki)에서는 저항(抵抗)의 증대(増大)가 완만하였지만, 발근곤란(発根困難)한 수종(樹種)(Quercus glauca, Pasania edulis, Rhaphiolepis umbellata Mak var integerreima)에 있어서는 삽목후(揷木後) 시일(時日)이 지남에 따라 급격(急激)히 증가하였다. 2. 잎을 제거한 경전체(茎全体)의 저항(抵抗)(RS)은 발근용역(発根容易)한 수종(樹種)에 있어서는 삽목후(揷木後) 시일(時日)이 지남에 따라 급격(急激)히 증가하였으며 발근곤란(発根困難)한 수종(樹種)의 경전체(茎全体)의 저항(抵抗) (RS)은 거의 증가하지 않았다. 3. 절단면부위(切断面部位)의 경의 저항(抵抗)(RC)은 발근용역(発根容易)한 수종(樹種)에서는 RC는 급격(急激)히 증가하고 있지만 발근곤란(発根困難)한 수종(樹種)에서는 거의 증가(増加)하지 않았고, 잎을 제거한 경전체(茎全体)의 저항(抵抗)(RS) 절단면부위(切断面部位)의 경(茎)의 저항(抵抗)(RC)의 변화(变化)에 기인하고 있었다. 4. 발근곤란(発根困難)한 수종(樹種)에 있어서는 엽(葉)의 엽액부(葉腋部)의 저항(抵抗)(RL)이 가장 컸으며, 삽수(揷穗)의 전체(全体) 저항(抵抗)(RT)의 대부분(大部分)을 RL가 점하고 있었다.

• 복식문화연구
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• 제8권2호
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• pp.237-260
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• 2000

According to the study of Jik-Ryoung(直領) consulting chronicles of the Chosun Era, Jik-Ryoung had been worn for various uses as official outfit, ordinary attire or clothes for the celebration of their coming of age, wedding ceremonies, funeral rites, and religious ceremonies, etc. from the beginning to the end of the Chosun Era. The conclusions are shown briefly as follows. 1. There are several terms of Po(袍) related to Jik-Ryoung in the chronicles under the name of Jik-Ryoung Ui(直領衣), Ui-Sal Jik-Ryoung(衣撒直領), and Jik-shin(直身). Jik-Ryoung Ui is the other name of Jik-Ryoung that they called it when it was used for funeral rites and religious ceremonies. The chinese Ye-Sal(曳撒) was called the Ui-Sal Jik-Ryoung in Korea, but this is different with Jik-Ryoung regarding its divided up and bottom style. Jik-Shin is almost same as Jik-Ryoung. 2. During the latter period of the Chosun Era, we can find diferent frequency in use of the Jik-Ryoung. Jik-Ryoung was shown constantly in the cases of that ding, Chinese Prince and lower-level constantly in the cases of that king, Crown Prince and lower-level officials wore it for funeral rites and lower-level officials, artisans, merchants, humbles and slaves wore it for official outfit. Uses of the Jik-Ryoung increased for military officers'outfits, in contrast to decreasing of uses for ordinary attires of king, Crown Prince, and the commons, and official outfits of civil officials. 3. These different aspects mean the change of estate and role. For the basic four ceremonial occasions the ceremonies of coming of age, marriage, funeral, and ancestor memorial-, it appeared constantly. Therefore the social role had been maintained also by then. As an official garb, the role for official uniform of petty official maintained by the end of the Dynasty. But from the latter 1600's to the former 1700's, the roles for official garbs of civil officials and military officers decreased and increased respectively. Before the Hideyoshi's Invasion of Korea in 1592, ordinary social clothes had orders by people's social status who wore them ; those were Dan Ryoung(團領), Hong Jik Ryoung(紅直領), Jik Ryoung(直領), Cho'l Rick(철릭) in the order named. After the war, various Po(袍), Shim Ui(深衣), Jung Chi Mak(中致莫), Chang Ui(　衣), Jang Ui(長衣), Ju Ui(周衣) and so on had been worn until the King Young Jo(英祖)·Jung Jo(正祖) period. In result, the social role of Jik-Ryoung was reduced as the uses decreased more and more. For a mourning dress, it had a same aspect as the case of ordinary social wear. 4. Considering the color, they used blue for the clothes for doing-up-the-hair ceremony, white for mourning clothes, and white, black for ancestor memorial ceremony clothes. On the official outfits of officials, dark blue and black were used mostly. And lower-level officials'clothes had white, red, and green on them. They used red and green for the plain dresses. 5. Examining the materials, clothes for the celebration of one's coming of age were made of high quality silks, Kwang Hwa Dan(廣禾緞). Also, they made clothes for funeral rites of rough and thick linen, and made clothes for religious ceremonies of linen and hemp. The official outfits were made of practical materials like cotton, hemp and ramie. Cotton, pongee and satin were used to make ordinary attire.

• 복식문화연구
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• 제8권6호
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• pp.915-929
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• 2000

As a result of research, the character of the general dress-costumes from the late 17th century to the early 18th century in Genre Paintings of DuSeo Yun and YoungSeok Cho is as follows. Firstly young women wore braided hair at the back of head and married women wore hair in the style of Unjeun-mori with Gache. The length of Jeogori (Korean traditional jacket) covered waistor shortened to waist length. The width of Jeongori was suitable, because side-seam line was straight or oblique as it comes into inner line. The width of Git was enough and Mokpan-git (shape of board) and Kal-kit (shape of knife) appeared, and sometimes used other color fabric. Sleeve was narrow and ostly folded up. Chima (Korean traditional skirt) used darker color fabric than Jeogori and the width of Chima was narrow and its length was short. Sokbaji (Korean traditional underpants) shown below were narrow and its end narrower. They were barefoot or they wore Hye (Korean traditional shoes) after putting on Beoseon (Korean traditional socks). Secondly general man's hair tie a topknot (sangtu) and put on headdres, 'Bang-lip', 'Mang-geon', hairband, 'Tang-geon' on head. The length of Jeogori became shorter from the line which covered hip to the line which covered waist. The width of Jeogori was suitable and sometimes it had a slit of side-seam line. The width of Git (neckand) was wide and the length of Git was long. 'Kal-git'appeared and it used other color fabric. The shape of sleeve was straight and narrow. They folded up their sleeves. They folded up their sleeves. They folded up their slack that look like 'Jam-bang-i'and the width of slacks was not suitable, and it was narrow. Baji (Korean traditional pants) were with or without knot, worn 'Hangjeon'(ankle band). They were barefoot and wore 'Hye'or 'Jipsin'(Korean traditional straw shoes). Thirdly a person of high birth or a low-ranked official put 'Yu-geon', 'Mang-geon', 'Gat', 'Tang-geon', 'Bok-du', 'Bok-geon', 'Whi-hang'on their head on a topknot. They wore 'Po (Shim-ui, Jick-ryeong'Jung-chi-mak, Do-po, etc)'on Baji and Jeogori. 'Po'was long and wide, it knot with 'Se-jo-dae'(string belt) or 'Po-baek-dae'(band belt). It had a slit of sideline and 'Mu'which had or had not or which were hard to confirm. The shape of sleeve was straight or very wide and its length was long. The width of Baji was wide and knotted with 'Hangjeon'and wore'Beoseon'and 'Hye'. Fourthly child's hair was short or knotted to the back of the head. The length of Jeogori reached waist line and its width was wide. It had a 'Jeogori'which had s slit of sideline. The shape of sleeve was 'Tong-su'(straight), and the length of sleeve was diverse. They put 'Baeja'on 'Jeogori'. The width of Baji was not wide. They wore them straight without or with knot, 'Hangjeon'. They were barefoot or put on 'Jipsin'.