• 한국식품조리과학회지
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• 제33권3호
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• pp.292-299
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• 2017

Purpose: The present study explored cookie making performance using Jeju magma seawater to elucidate the effects of minerals in water on quality of baked goods. Methods: Seven water samples were analyzed for their mineral content, pH and water hardness. Starch pasting properties of flour in water samples was analyzed using RVA, and cookie making performance using water samples was evaluated with the AACCI wire-cut cookie baking method. Quality of cookies was measured by weight loss during baking, cookie geometry, color, and firmness. Results: Hardness of water samples ranged from 0-4200, and mineral content was in the order of magma seawater > 100% ED mineral water > 50% ED mineral water > 10% ED mineral water > tap water > Samdasoo > distilled water. RVA results showed that water hardness exhibited significant relationships with pasting temperature (p<0.05, R=0.863), peak viscosity (p<0.001, R=0.944), final viscosity (p<0.05, R=0.861), and setback (p<0.05, R=0.782). Cookie baking results showed that cookie diameter increased in the order of magma seawater < 100% ED mineral water < 50% ED mineral water < 10% ED mineral water $\approx$ tap water < Samdasoo < distilled water. Conclusion: As mineral content in water increased, flour pasting temperature and viscosity increased, whereas cookie diameter decreased with color fading. However, cookies formulated with 50% ED mineral water showed similar cookie geometry and texture to those with tap water. Therefore, controlling the mineral content of water can be successfully applied to produce mineral-enriched cookies.

• ALGAE
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• 제34권2호
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• pp.177-185
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• 2019

Ecklonia cava is popular in Korea as a marine functional materials. E. cava is generally collected and used on the coast of Jeju Island. However, the continuous use of collected natural E. cava may be limited because difficult to secure throughout the year and may be exposed to environmental pollution. Jeju magma seawater (MSW) was known to be significant advantages such as safety, cleanness, stability, and functional improvement. Attempts have been reported on application of MSW to the culturing of macro- and microalgae and showed improved results. Thus, the objective of the present study was to explore the anti-melanogenesis activity of brown seaweed E. cava (E. cava cultured with MSW [MSWE]) extract cultured in tanks with MSW of Jeju Island to evaluate the possibility of cosmeceutical industrial application. MSWE extract showed the higher polyphenolic and dieckol contents than natural E. cava (NE) extract. Anti-melanogenesis activity of MSWE extract and NE extract are tested and compared using tyrosinase and dihydroxyphenylalanine (DOPA) oxidation inhibition assay. MSWE extracts evidenced more effective tyrosinase and DOPA oxidation inhibition activity than that of the NE extracts and the commercial whitening agent, arbutin. MSWE extracts also markedly inhibited melanin synthesis and decreased the expression of melanogenesis-related protein in ${\alpha}$-melanocyte stimulating hormone-stimulated B16F10 melanoma cells without cytotoxicity. These results suggest that MSW cultivation process would be more effective in releasing bioactive compounds with whitening effect from seaweed such as E. cava at an industrial scale.

• Journal of Plant Biotechnology
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• 제39권4호
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• pp.281-287
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• 2012

본 연구에서는 용암해수가 지닌 독특한 미네랄 구성을 바탕으로 자란의 생육에 적합한 농도를 규명하기 위해 실험을 수행하였다. 자란의 기내배양용 고체배지구성을 위해 30 g/L sucrose와 3.0과 8.0 g/L의 gelrite 및 plant agar를 동일하게 처리한 MS 배지와 RO(Reverse Osmosis) 탈염수, ED(Electrodialsis) 탈염수 배지를 각각 제조하여 배지경도를 측정한 결과, plant agar가 첨가된 MS, RO, 및 ED 배지의 경도는 각각 60,000, 40,000 및 $38,000g/cm^2$의 경도가 측정되었다. 하지만 gelrite가 첨가된 배지는 MS를 제외한 RO, ED배지는 $2,000g/cm^2$ 내외의 경도를 보였다. 제조된 배지에서 식물체의 생육을 확인한 결과, RO 탈염수 0%에서는 88%, RO 50%에서는 57%, RO 100%에서는 31%의 생존율을 보였다. 하지만 잎 길이, 엽폭은 모두 RO 탈염수 100%에서 높았다. ED배지는 ED 100%를 제외한 배지에서 모두 양호한 생장을 보였다. 엽록소 함량 분석을 통한 생리적 건전도를 측정한 결과, 대조구에서는 총엽록소 함량이 66.3 ug/ml인데 비해 RO 10%, RO 50%, RO 100%, ED 10%, ED 50%, ED 100%에서는 각각 77.3, 69.3, 76.4, 79.0, 75.7, 74.4 ug/ml로 모두 대조구보다 높은 총엽록소 함량을 나타냈다. 또한 용암해수 내 유용성분의 식물체내로의 축적은 스토론튬, 바나듐, 셀레늄 등이 원활하게 이루어짐을 확인한 바, 용암해수를 이용한 식물조직배양용 배지로의 산업적 이용가치가 높을 것으로 보이고 더 나아가 용암해수가 원예작물뿐만 아니라 유용성분 함유 약용작물의 생산에도 충분히 활용 가능할 것으로 사료된다.

• 한국응용과학기술학회지
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• 제37권3호
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• pp.448-462
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• 2020

식이 영양에 의한 충분한 영양분 섭취는 치매에 의한 뇌 인지 기능의 저하의 위험성을 줄일 수 있는 중요한 수단이다. 설포라판은 뇌 인지 기능 개선효과가 있는 것으로 알려져 있는 영양 성분으로, 설포라판이 다량으로 포함된 브로콜리 추출물은 인지 기능 개선 효과가 좋을 것으로 기대된다. 본 연구에서는 제주도 탈염 용암해수 재배로 제조한 브로콜리 추출물이 뇌 인지 기능 개선 효과를 가지고 있는지 시험하였다. 제주도 용암해수는 유용 미네랄 (아연, 바나듐, 게르마늄)이 풍부해서, 용암 해수에서 재배한 작물은 유용미네랄 함량이 높은 것으로 알려 져 있다. 제주도 탈염 용암 해수를 사용해서 재배한 브로콜리 추출물의 화학 조성 중 설포라판과 글루코라판 성분을 분석하기 위해 LC-Q-orbitrap 질량 분석기를 사용하였고, 설포라판의 정량 분석을 위해 HPLC 를 사용하였다. 브로콜리 추출물의 신경 세포 사멸 억제 효과와 항 염증 효과를 시험하기 위해 SH-SY5Y 세포를 사용한 실험을 수행하였고, 시냅스 가소성 촉진 효과를 시험하기 위해 SH-SY5Y 세포에서 시냅스 가소성 관련 단백질의 발현 변화와 아세틸콜린 분해 효소의 활성 변화를 측정하였다. 이러한 실험들을 수행한 결과 탈염 염지하수로 재배한 브로콜리 추출물은 신경 세포 사멸 억제 효과와 항염증 효과가 있음을 확인 하였고, 시냅스 가소성 관련 단백질 발현을 증가 시키고 아세틸콜린 분해 효소의 활성을 억제해서 시냅스 가소성을 증가 시키는 효과가 있는 것을 확인 하였다. 이상의 결과들은 제주도 탈염 용암해수로 재배한 브로콜리 추출물이 치매에 의한 뇌 인지 기능 저하를 억제하는 좋은 식이 영양분으로 사용될 수 있는 가능성을 제시하였다.

• Nutrition Research and Practice
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• 제4권1호
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• pp.3-10
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• 2010

This study was performed to investigate the effect of desalinated underground seawater (named as 'magma seawater', MSW) of Jeju Island in Korea on lipid metabolism and antioxidant activity. MSW was collected from underground of Han-Dong in Jeju Island, and freely given to high fat diet (HFD)-fed C57BL/6 mice for 10 weeks. Although there were no significant differences in the body weight changes and plasma lipid levels, hepatic triglyceride levels were significantly lower in the MSW group than in the normal tap water (TW)-drunken control group. Furthermore, the activity of fatty acid synthase (FAS) was significantly decreased and carnitine palmitoyltransferase (CPT) activity was increased in MSW group compared to TW group. Similarly, real-time PCR analysis revealed that mRNA expressions of lipogenic genes were lowered in MSW groups compared to the control group. In a morphometric observation on the liver tissue, accumulation of fats was remarkably reduced in MSW group. Meanwhile, in vitro assay, tree radical scavenging activity measured by using diphenylpicrylhydrazyl (DPPH) was increased in MSW group. The 2'-7'-dichlorofluorescein diacetate (DCF-DA) staining followed with fluorescent microscopy showed a low intensity of fluorescence in MSW-treated HepG2 cells, compared to TW-treated HepG2 cells, which indicated that the production of reactive oxygen species by tert-butyl hydroperoxide (t-BHP) in HepG2 cells was decreased by MSW treatment. The antioxidant effect of MSW on t-BHP-induced oxidative stress in HepG2 cells was supported by the increased activities of intracellular antioxidant enzymes such as catalase and glutathione reductase. From these results, we speculate that MSW has an inhibitory effect on lipogenesis in liver and might play a protective role against cell damage by t-BHP-induced oxidative stress.