• Journal of Applied Biological Chemistry
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• 제42권3호
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• pp.119-124
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• 1999

About two thirds of the naturally occurring antibiotics have been discovered from actinomycetes. Therefore, the probability of discovering further new antibiotics from actinomycetes is declining as many known metabolites are isolated repeatedly. However, various efforts leave been made in order to enhance the probability of discovering novel compounds. In the present study, we have developed new screening strategies based on the antibiotic biosynthetic pathway, and the genetic information, utilizing polymerase chain reaction. We have selected macrolide type polyketides. In order to divide the ansamycin group antibotic of macrolide type polyketides, we have selected 3-amino-5-hydroxybenzoic acid (AHBA) moiety which contains a biosynthetically unique structural element in the group as a target molecules. Oligonucleotide primers were designed to amplify DNA fragments of macrolide type polyketide synthase and AHBA synthase genes from fourteen actinomycetes species. This method was successfully applied to all three of the known macrolide type polyketide produccing actinomycetes tested. In addition, it also identified the presence of potential macrolide type polyketide producing genes from seven actinomycetes that were known to produce none of macrolide type polyketides, and AHBA biosynthetic genes in one actinomycetes. This technique is potentially useful for the screening of new antibiotices and cloning of their biosynthetic genes.

• KSBB Journal
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• 제22권4호
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• pp.218-221
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• 2007

본 연구에서는 초음파 파쇄기를 이용하여 폴리엔 항진균 활성을 갖는 방선균 배양액으로부터 pellet을 회수하여 미생물농약으로 사용이 용이한 포자를 확보하기 위한 최적화 연구를 수행하였다. 그 결과, Streptomyces sp. MMBL001, MMBL003 그리고 Pseudonocardia autotrophica는 각각 12%, 18%, 12% 출력 (100%, 700 W)에서 최적의 포자 전환율을 보임을 확인하였다. 이렇게 확보한 포자를 10% skim milk 보호제를 사용하여 동결건조한 후 각각의 생균수를 측정한 결과, MMBL003이 MMBL001과 P. autotrophica에 비해 3배 이상의 생균수가 유지됨이 측정되었다. C. albicans를 대상으로 항진균 효과를 확인한 결과, 동결건조된 3종의 방선균주 모두 우수한 항진균 활성을 유지함으로써 신규 폴리엔 생산 방선균을 이용한 미생물농약으로서의 활용 가능성을 제시하였다.