• 미생물학회지
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• 제50권3호
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• pp.249-253
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• 2014

THOC7/Mft1는 mRNA가 전사되는 동안 mRNP의 포장과 mRNA 방출에 관여하는 진화적으로 잘 보존된 THO 복합체의 구성인자이다. 분열효모 Schizosaccharomyces pombe에서 THOC7/Mft1의 이종상동체(spThoc7)가 합성치사 돌연변이체 SLRsm1의 생장 결함을 부분적으로 상보하는 것으로 선별되었다. 이배체 S. pombe 균주에 하나의 spthoc7 유전자만을 결실시킨 후 4분체 분석을 수행한 결과, 이 유전자는 생장에 필수적이지 않았다. 하지만, ${\Delta}thoc7$ 결실돌연변이는 생장과 mRNA의 핵에서 세포질로의 방출에 약간의 결함을 보였다. 기능을 하는 spThoc7-GFP단백질은 주로 핵 안에 존재하였다. 이와 같은 결과들은 spThoc7도 mRNA 방출에 관여하고 있음을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제24권5호
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• pp.714-718
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• 2014

Apoptosis is the process of programmed cell death executed by specific proteases, the caspases, which mediate the cleavage of various vital proteins. Elucidating the consequences of this endoproteolytic cleavage is crucial to understanding cell death and other related biological processes. Although a number of possible roles for caspase-6 have been proposed, the identities and functions of proteins that interact with caspase-6 remain uncertain. In this study, we established a cell line expressing tandem affinity purification (TAP)-tagged caspase- 6 and then used LC-MS/MS proteomic analysis to analyze the caspase-6 interactome. Eight candidate caspase-6-interacting proteins were identified. Of these, five proteins (hnRNP-M, DHX38, ASPP2, MTA2, and UACA) were subsequently examined by co-immunoprecipitation for interactions with caspase-6. Thus, we identified two novel members of the caspase-6 interactome: hnRNP-M and MTA2.

• 미생물학회지
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• 제51권2호
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• pp.181-185
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• 2015

Tho2/THOC2는 전사 과정을 mRNA 성숙 및 방출과 연결함으로써 mRNP의 생성에 중요한 역할을 담당하는 THO 복합체의 구성인자이다. 분열효모 Schizosaccharomyces pombe의 유전체 데이터에서 Tho2/THOC2의 이종상동체를 찾아 기능을 분석하였다. 4분체 분석 결과 이 유전자는 생장에 필수적이었다. S. pombe tho2 유전자의 발현을 억제하거나 과발현시키면 생장이 저해되는데, 세포의 길이가 길어지고 비정상적인 DNA분포와 $poly(A)^+$ RNA가 핵 안에 축적되는 표현형을 보였다. 또한 정상적인 기능을 가진 GFP-Tho2 단백질은 주로 핵 안에 존재하였다. Yeast two-hybrid 분석에서 Tho2는 THO 복합체의 또 다른 구성인자인 Tex1과 상호작용을 하였다. 이와 같은 결과들은 S. pombe의 Tho2 상동체도 THO 복합체의 구성인자로 mRNA 방출에 관여하고 있음을 시사한다.

• 대한화학회지
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• 제43권3호
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• pp.307-314
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• 1999

대장균의 RNase P의 RNA 성분인 M1 RNA는 대장균 rnpB 유전자의 주요한 일차전사물인 선구-M1 RNA로부터 3'가공으로 생성된다. 이 가공 활성을 가지고 있는 효소 분획을 부분 정제하고 그 특성을 조사하였다. 이 활성 분획을 높은 염농도에 노출시키면 가공 활성이 불활성화하는 것으로 보아, 가공효소는 여러 효소로 이루어진 효소 복합체인 것으로 추정된다. 이 효소 분획은 화학적 핵산 가수분해효소인 납(II) 이온으로 처리하면 효소 활성을 잃지만, 효소 분획 자체에서 추출한 RNA를 가하면 효소 활성을 되찾는다. 이 결과는 효소 활성에는 RNA 분자가 필요하다는 것을 시사한다. 부분 정제한 효소로 형성되는 절단자리들의 분석 결과도, 3'가공과정이 여러 효소에 의하여 일어나고, 적어도 두 가지 다른 경로로 일어난다는 것을 암시한다.

• Journal of Veterinary Science
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• 제22권5호
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• pp.62.1-62.13
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• 2021

Background: Canine mammary gland tumor (MGT) is the most common cancer in aged female dogs. Although it's important to identify reliable metastasis or prognostic factors by evaluating related to cell division, adhesion, and cancer stem cell-related transcription factor (TF) in metastasis-induced canine MGT, but there are limited studies. Objectives: We aimed to identify metastasis prognostic factors and cancer stem cell-TFs in canine MGTs. Methods: Age-matched female dogs diagnosed with MGT only were classified into metastatic and non-metastatic groups by histopathological staining of MGT tissues. The mRNA levels of cancer prognostic metastasis molecular factors (E-cadherin, ICAM-1, PRR14, VEGF, HPRT1, RPL4 and hnRNP H) and cancer stem cell-related TFs (Oct4, Sox2, and Nanog) were compared between metastatic and non-metastatic canine MGT tissues using qRT-PCR analysis. Results: The mRNA levels of ICAM-1, PRR14, VEGF, hnRNP H, Oct4, Sox2, and Nanog in metastatic MGT group were significantly higher than those in non-metastatic MGT group. However, mRNA level of RPL4 was significantly lower in metastatic MGT group. Loss of E-cadherin and HPRT1 was observed in the metastatic MGT group but it was not significant. Conclusions: Consistent expression patterns of all metastasis-related factors showing elevation in ICAM-1, PRR14, VEGF, hnRNP H, Oct4, Sox2, and Nanog, but decreases in RPL4 levels occurred in canine MGT tissues, which was associated with metastasis. Thus, these cancer prognostic metastasis factors and TFs of cancer stem cells, except for E-cadherin and HPRT1, can be used as reliable metastasis factors for canine MGT and therapeutic strategy.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.198.1-198
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• 2000

No Abstract, See Full Text

• Molecules and Cells
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• 제39권12호
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• pp.841-846
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• 2016

Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.

• 대한안전경영과학회지
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• 제6권1호
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• pp.11-23
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• 2004

The modem industrial society is developing while growing more diverse and gigantic. Accordingly, occupational injuries or accidents can be caused in various situations, not just in the limited range of workplaces but also in the surroundings, and interest has increased in the prevention of occupational accidents with respect to occupational health and safety, and environment. Thus, this thesis will consider 4MlE (Man, Machine, Method, Material, Environment) as the fundamental causes of accidents and introduce a model of system in which the output of the process control system is replaced by accidents with its input by 4M1E. Furthermore, it will demonstrate how occupational hazardousness can be measured, whereby it can also be rated, by examining the relationship between 4M1E and types of accident in terms of the categories of severity, frequency, and detectability, based on the application of the model to the framework of FMEA.

• Asian Pacific Journal of Cancer Prevention
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• 제17권12호
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• pp.5229-5235
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• 2016

Prostate cancer (PCa) remains one of the most widespread and perplexing of all human malignancies. Assessment of gene expression is thought to have an important impact on cancer diagnosis, prognosis and therapeutic decisions. In this context, we explored combined expression of PCa related target genes AMACR and PCA3 in 126 formalin fixed paraffin embedded prostate tissues (FFPE) from Moroccan patients, using quantitative real time reverse transcription-PCR (RT-qPCR). This quantification required data normalization accomplished using stably expressed reference genes (RGs). A panel of twelve RG was assessed, data being analyzed using GenEx V6 based on geNorm, NormFinder and statistical methods. Accordingly, the hnRNP A1 gene was identified and selected as the most stably expressed RG for reliable and accurate gene expression quantification in prostate tissues. The ratios of both PCA3 and AMACR gene expression relative to that of the hnRNP A1 gene were calculated and the performance of each target gene for PCa diagnosis was evaluated using receiver-operating characteristics. PCA3 and AMACR mRNA quantification based on RT-qPCR may prove useful in PCa diagnosis. Of particular interesting, combining PCA3 and AMACR quantification improved PCa prediction by increasing sensitivity with retention of good specificity.

• Molecules and Cells
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• 제27권6호
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• pp.657-665
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• 2009

The ovomucoid pre-mRNA has been folded into mini-hairpins adaptable for the RNA recognition motif (RRM) protein binding. The number of mini-hairpins were 372 for pre-mRNA and 83-86 for mature mRNA. The spatial arrangements are, in average, 16 nucleotides per mini-hairpin which includes 7 nt in the stem, 5.6 nt in the loop and 3.7 nt in the inter-hairpin spacer. The constitutive splicing system of ovomucoid-pre-mRNA is characterized by preferred order of intron removal of 5/6 > 7/4 > 2/1 > 3. The 5' splice sites (5'SS), branch point sequences (BPS) and 3' splice sites (3'SS) were identified and free energies involved have been estimated in 7 splice sites. Thermodynamic barriers for splice sites from the least (|lowest| -Kcal) were 5, 4, 7, 6, 2, 1, and 3; i.e., -18.7 Kcal, -20.2 Kcal, -21.0 Kcal, -24.0 Kcal, - 25.4 Kcal, -26.4 Kcal and -28.2 Kcal respectively. These are parallel to the kinetic data of splicing order reported in the literature. As a result, the preferred order of intron removals can be described by a consideration of free energy changes involved in the spliceosomal assembly pathway. This finding is consistent with the validity of hnRNP formation mechanisms in previous reports.