• Plant Biotechnology Reports
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• 제3권1호
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• pp.67-74
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• 2009

An efficient micropropagation protocol based on multiple shoot induction and callus regeneration has been standardized in Sarcostemma brevistigma, a rare medicinal plant. The nodal cuttings were cultured on MS medium supplemented with BA ($0.5-8{\mu}M$) or Kn ($0.5-8{\mu}M$) alone or in combination with NAA ($0.5-1.5{\mu}M$). Maximum multiple shoot induction was observed on MS medium supplemented with $4{\mu}M$ BA. On this medium, 100% cultures responded with an average number of 11.3 shoots per explant. However, the average shoot length was limited to only 0.9 cm on this medium. The addition of $1{\mu}M$ NAA along with $4{\mu}M$ BA gave rise to an average number of 10.9 shoots with an average shoot length of 1.8 cm. Luxuriantly growing callus was obtained on MS medium supplemented with BA ($5{\mu}M$) and 2,4-D ($2{\mu}M$). The callus was subcultured on MS medium supplemented with BA ($2-15{\mu}M$) or Kn ($2-15{\mu}M$) alone or in combination with NAA ($0.5-2{\mu}M$) for shoot organogenesis. Optimum callus regeneration was obtained on MS medium supplemented with $10{\mu}M$ BA and $1{\mu}M$ NAA. On this medium, 100% cultures responded with an average number of 13.4 shoots per culture. The shoots obtained via multiple shoot induction and organogenesis were rooted on half-strength MS medium supplemented with NAA ($1-7{\mu}M$) or IBA ($1-7{\mu}M$). IBA was better than NAA in terms of both the percentage of cultures that responded and the average number of roots per explant. The rooted shoots were successfully transplanted to soil with 86% success. This standardized protocol will help to conserve this rare medicinal plant.

• 농업과학연구
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• 제23권1호
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• pp.159-176
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• 1996

This study is aimed at reviewing the methods of joint cost allocation and allocating the joint cost of estuary dam with specially repect to Kumgang Large-scale Agricultural Comprehensive Development Project. Apart from the water resource development project propelled by Water Resource Development Corporation in connection with Law of Multipurpose Dam Development, the Largescale Comprehensive Agricultural Development Projects couldn't ins-titutionally be carried out cost allocation of common facilities, even though it were concerned with irrigation, municipal and industrical water supply, flood control, sightseeing and industrial zone development components. To decrease farmer's burden of the project costs and, operation and maintenance costs, the joint costs of common facilities like estuary dam included in agricultural development projects have to be allocated by suitable method as alternative cost-remaining benefit method and the analytical activity should be supported by revising the concerned laws as Rural Development and Promotion and, Rural Rearrangement conpatible with the law for multipurpose dam development. Kumgang Agricultural Comprehensive Development Project was selected as a case study for the estimation of socio-economic benefits by project components and joint cost allocation of the estuary dam. The main results of the study are as follows; Joint cost allocation and unit charges by components 1. The project area will be 25,554ha with total project cost of 624,860 million won including the estuary dam cost of 120,843 million won. The project costs were ex-pressed by 1994 constant price. 2. Total quantity of water was estimated 365 million tons which were consisted of 245 million tons for irrigation, 73 million tons for municipal water and 47 million tons for industrial water. 3. The rates of joint cost allocation were amounted to 34.2% for agriculture, 2.5% for sightseeing, 45.7% for transportation, 11.8% for M & I water supply and 5.8% for flood control respectively. 4. The unit financial charges by project components were estimated at 7.88 won per ton for irrigation, 16.11won for M & I water, 1,686won per vehicle one pass, 977won per Pyeong according to the capital recovery method. The financial charges using straitline method for depreciation were estimated at 7.88won per ton for irrigation, 9.12won per ton for M & I water, 624won per vehicle one pass for transportation and 331won per Pyeong for sightseeing area. 5. The unit economic charges by project components were estimated at 21.1 won per ton for irrigation, 15.2won for M & I water, 977won per vehicle one pass, 977won per Pyeong according to the capital recovery method. The economic charges using straitline method for depreciation were estimated at 11.72won per ton for irrigation, 8.61won per ton for M & I water, 331won per vehicle one pass for transportation. Policy recommendation 1. The unit operation and maintenance costs for irrigation water in the paddy field couldn't be imposed as the water resource cost untreated. 2. The dam costs including investment cost and O & M cost, as a joint cost, had to be allocated by each benefited components as transportation, M & I water supply, flood control, irrigation and drainage, and sightseeing. But the agricultural comprehensive project have been dealt as an irrigation project without any appraisal socio-economic benefits and any allocating the joint cost of estuary dam. 3. All the associated project benefits and costs must be evaluated based on accounting principle and rent recovery rate of the project costs and O & M costs should be regulated by the laws concerned. 4. The rural development and promotion law and rural rearrangement law have to be revised comprising joint cost allocation considering free rider problems. 5. The government subsidy for the agricultural base development project has to be covered all the project costs. In case of common facilities representing joint cost allocation problems, all the allocated casts for other purposes like transportation and M & I water supply etc. should be recovered for formation in investment fund for agricultural base development and to procure O & M costs for irrigation facilities.

• Clinical and Experimental Reproductive Medicine
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• 제24권2호
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• pp.217-223
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• 1997

This study was performed to determine if women with day 3 serum inhibin-B concentrations <45pg/ml (conversion factor to SI unit, 1.00) demonstrate a poor response to ovulation induction and assisted reproductive technology outcome to women with inhibin-B${\ge}45pg$/ml, independant of day 3 FSH, E2 and patient age. From Jan 1996 to Dec 1996, 16 volunteers patients who underwent 25 IVF cycles with luteal phase GnRH agonist suppression and HMG stimulation were allocated to the study group. We evaluated day 3 serum inhibin-B, FSH, E2, peak E2, cancellation rate per initiated cycle (%) and clinical pregnancy rate per initiated cycle (%) according to the above two groups and independent of patient age, day 3 FSH, day 3 E2 and all of above combined. Women with day 3 serum inhibin-B${\ge}45pg$/ml demonstrated higher average day 3 inhibin-B level, clinical pregnancy rate per initiated cycle ($20.3{\pm}2.5$ pg/ml vs $80.9{\pm}5.0$ pg/ml, p<0.05; 24.8% vs 8.5%, p<0.05) and lower day 3 FSH level, cancellation rate per initiated cycle ($6.9{\pm}0.3$ mIU/ml vs $8.5{\pm}0.5$ mIU/ml, p<0.05; 1.5% vs 9.0%, p<0.05). Women with day 3 serum inhibin${\ge}45pg$/ml and age<40 year demonstrated higher pregnancy rate per initiated cycle (28.2% vs 7.4%, p<0.05) and lows. day 3 FSH level, cancellation rate per initiated cycle ($6.9{\pm}0.5$ mIU/ml vs $8.2{\pm}0.7$ mIU/ml, p<0.05; 1.0% vs 9.0%, p<0.05). Women with day 3 serum inhibin${\ge}45pg$/ml and day 3 FSH<15mIU/ml demonstrated higher pregnancy rate per initiated cycle (33.5% vs 9.5%, p<0.05) and lower day 3 FSH level, cancellation rate per initiated cycle ($7.7{\pm}0.2$ mIU/ml vs $8.5{\pm}0.5$ mIU/ml, p<0.05; 1.5% vs 10.0%, p<0.05). Women with day 3 serum inhibin${\ge}45pg$/ml and day 3 E2<50pg/ml demonstrated higher pregnancy rate per initiated cycle (30.0% vs 9.5%, p<0.05) and lower cancellation rate per initiated cycle (1.5% vs 9.5%, p<0.05). Women with day 3 serum inhibin${\ge}45pg$/ml, age<40 year, day 3 FSH<15mIU/ml and day 3 E2<50pg/ml demonstrated higher pregnancy rate per initiated cycle (30.0% vs 10.8%, p<0.05) and lower day 3 FSH level, cancellation rate per initiated cycle ($6.8{\pm}0.6$ mIU/ml vs $8.4{\pm}0.9$ mIU/ml, p<0.05; 1.5% vs 7.8%, p<0.05). Therefore women with low day 3 serum inhibin-B concentrations demonstrate a poorer response to ovulation induction and are less likely to conceive a clinical pregnancy though ART relative to women with high day 3 inhibin-B and day 3 serum inhibin-B, in addition to a day 3 FSH, E2 and patient age, appears helpful in prediction in IVF-ET outcome.

• 한국수산과학회지
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• 제13권2호
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• pp.45-56
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• 1980

한산${\cdot}$거제만 굴 양식장에 관한 $1970\~1979$년까지 사이에 조사된 여러 가지 기존 자료와 1978년에 조사한 몇 가지 저질에 관한 조사자료를 토대로 한 굴양식장의 양식밀도에 관한 연구 결과는 다음과 같다. 1. 각 양성장에 있어 양성중인 굴밀도와 양성장 저이중에 함유되어 있는 COD, 페오파이틴의 양으로부터 구한 저이오염도간에는 상관이 있었다. 저이오염도 12를 한산${\cdot}$거제만의 한계치로 하여 평균 수심에 따른 단위면적당 굴 뭉치 수(C)와 저이오염도 (M)간의 회선직선식은 뗏목식의 경우 M=2.7341+1.8945C, 로우프식의 경우 M=6.4565+1.0988C에 적용하여 추정된 적정 양식밀도는 수하연수로 $m^2$당 뗏목식 0.12연, 로우프식 0.25연이었다. 2. $1970\~1978$년간의 양식결과에서 뗏목당 수하연수가 $350\~558$연 사이에서는 수하연수가 많을 수록 뗏목당 생산량은 많았고, 뗏목 1태가 점유한 수면적이 $1,000\~6,000m^2$까지는 태당 점유면적의 증가에 따라 생산량도 증가하였다. 뗏목당수하연수$(S_R)$와 뗏목당 생산량$(P_R)$ 관계식인 $P_R=-1.1272+0.009823S_R$와 뗏목 점유 면적$(D_R)$과 수하연당 생산량$(A_R)$의 관계식인 $A_R=4.7254+0.000604D_R$에 출하중심시기의 뗏목당 생산량 3.2t을 대입하여 구한 적정 양식밀도는 $m^2$당 약 0.11 연이다. 3. 단위면적당 수하연수(S)와 수하연당 생산량 (P/S)의 관계식인 $\frac{P}{S}=11.7213-44.2472S$에서 단위 면적당 수하연수가 많을수록 수하연당 생산량은 직선적으로 감소하였고, 이 식의 변형인 포물선식인 $P=11.7213\;S-44.2472S^2$에 의하면 수하연당 생산량은 $m^2$당 0.13연 수준에 달하기 까지는 증가하고 이 수준을 넘으면 오히려 감소한다. 이때 기대되는 최대 생산량은 약 5,600t으로 추정된다. 4. 양식밀도와 저이오염도, 뗏목당 수하연수와 뗏목당 점유면적에 따른 생산량 및 수하연 밀도와 연당 생산량의 3가지 측면에서 검토한 결과를 종합하건데, 한산${\cdot}$거제만 굴 양식장에 있어, 적정 양식밀도는 수하연수로 $m^2$당, 뗏목식에서 $0.11\~0.13$연, 로우프식에서 0.25연으로 추정된다. 5. 현재 한산${\cdot}$거제만의 굴 양식밀도는 수하연수로 $m^2$당, 뗏목식에서 0.24연, 로우프식에서 0.28연으로서 적정 양식밀도의 한계치를 초과하고 있다.

• 원예과학기술지
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• 제34권6호
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• pp.840-844
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• 2016

Quinoa (Chenopodium quinoa Willd.) is a grain crop with high nutritional value. The leaves and sprouts of quinoa can also be consumed either raw or cooked, providing considerably nutritional value as well as high antioxidant and anticancer activities. This study was carried out to obtain basic data to assist in the practical design of a plant factory with artificial lighting for the cultivation of quinoa as a leafy vegetable. We estimated the energy content of the quinoa and the electrical energy required to produce this crop. The yield was 1,000 plants per day, with a planting density and light intensity of $0.015m^2$ ($15{\times}10cm$) and $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. The total number of plants, cultivation area, and electricity consumption were estimated to be 25,000, $375m^2$, and $93,750{\mu}mol{\cdot}s^{-1}$, respectively. White fluorescent lamps were used at a power of 20.4 kW from 1,857 fluorescent lamps (FL, 55 W), and the cost for electricity was approximately 1,820 dollars (exchange rate of $1 = 1,200 won) per month. For a daily harvest of 1,000 plants per day in a closed plant factory, the estimated light installation cost, total installation cost, and total production cost would be 15,473, 46,421, and 55,704 dollars, respectively. The calculated production cost per plant, including labor costs, would be 27 cents for the 25-day cultivation period, with a marketable ratio of 80%. Considering the annual total expenses, income, and depreciation costs, the selling price per plant was estimated to be approximately 56 cents.

• Journal of Yeungnam Medical Science
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• 제6권1호
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• pp.151-163
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• 1989

건강한 성인 83명을 무작위로 선정하여 실시한 신경전도속도 검사에서 다음과 같은 결과를 얻었다. 1) 상지의 정중신경에서는 운동신경의 TL이 3.0-4.2msec 이고, MNCV는 각각 52.1-70.3m/sec(W-E), 44.6-71.0m/sec(E-Ax), 56.6-70.8m/sec(W-E), 50.5-75.1m/sec(E-Ax)이며, CNAP의 진폭은 $6.5-46.1{\mu}V$였다. 2) 척골신경에서는 운동신경의 TL이 2.4-3.4msec이고, MNCV는 각각 54.6-72.8m/sec(W-E), 41.1-64.9m/sec(E-Ax)이며, 진폭은 3.1-12.0mV였다. 척골신경의 MNCV는 각각 31.1-44.7m/sec(F-W)m, 55.9-70.9m/sec(W-E), 46.9-67.1m/sec(E-Ax)이며, CNAP의 진폭은 4.8-42.9${\mu}V$범위였다. 3) 요골신경에서는 운동신경의 TL이 1.9-2.7msec이고, MNCV는 53.1-77.5m/sec(W-E)이며, CMAP의 진폭은 1.1-6.6mV범위였다. 요골신경의 SNCV는 각각 38.5-52.1m/sec(F-V), 53.2-75.2m/sec(W-E) 이며, CNAP의 진폭은 $2.5-9.2{\mu}V$범위였다. 4) 하지의 비골신경에서는 운동신경의 TL이 3.5-5.7msec이며, MNCV는 각각 44.4-58.6m/sec(A-FH), 42.8-65.8m/sec(FH-PF)이며, CMAP의 진폭은 0.6-12.7mV 범위였다. 5) 후경골신경에서는 TL이 4.0-6.2m/sec이며, MNCV는 40.6-60.6m/sec이며, CMAP의 진폭은 3.9-29.2mV범위였다. 6) 비골신경의 SNCV는 37.5-49.5m/sec이며, CNAP의 진폭은 $0.7-17.1{\mu}V$범위였다. 7) H-반사의 평균 잠복기는 28.4msec였다.

• 한국축산시설환경학회지
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• 제1권2호
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• pp.145-153
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• 1995

두당돈방면적과 돈군의 크기 등 비기후적 환경요인이 육성돈의 생산과 각종 행동유형에 미치는 영향을 구명하기 위하여 체중 30kg부터 60kg까지의 육성돈을 18돈방에 총 270두를 공시하여 시험하였다. 두당돈방면적을 0.33$m^2$, 0.44$m^2$ 및 0.66$m^2$로 설정하였고 돈군의 크기는 12두, 15두 및 18두로 하였다. 증체량, 사료섭취량, 사료요구율 및 휴식, 채식, 공격, 사회성, 유희행동 등의 각종 행동유형을 조사하였으며 각 조사항목에 대하여 요인별로 주요인 효과와 자 요인들간의 상호작용 효과를 분석하였으며 그 결과를 요약하면 다음과 같다. 1. 두당 돈방면적을 0.44$m^2$로 한 것이 육성돈의 증체 및 사료요구율(p<0.01)면에서 가장 우수한 것으로 나타났다. 2. 돈군의 크기에 따라서는 15두군이 12두와 18두군에 비하여 증체와 사료요구율이 가장 우수한 것으로 나타났다(p<0.05). 3. 육성돈의 행동에 대한 두당돈방면적의 효과는 두당돈방면적이 좁아지면 휴식행동과 비공격적 사회성 친화 행동이 감소되나 섭취행동과 공격행동은 그 빈도가 증가되었다(p<0.01). 4. 돈군의 크기는 육성돈의 행동에 크게 영향을 미치고 있으며 돈군의 크기가 커질수록 육성돈의 공격행동과 섭취행동은 증가되나 휴식행동은 감소되었다(p<0.01). 5. 육성돈의 생산과 행동면에서 두당 0.44$m^2$로 하고 돈군의 크기는 15두로 하는 것이 최적조건인 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제7권1호
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• pp.49-55
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• 1994

This study was conducted to compare the of rearing native and cross-bred dairy cows and returns received from them. For this purpose, 144 cows of 132 household from 24 villages were randomly selected. of them, 96 were native cows and 48 were cross-bred cows. The study revealed that among the structure of cost components, labour charge occupied the major share in the total cost of milk production per litre. The total cost of rearing native and cross-bred cows was Tk. 14,155 and Tk. 19,854 per annum, respectively. The average net cost of milk production per litre was Tk. 14.12 for native cows and Tk. 0.52 and for cross-bred cows were Taka 3.40. The benefit-cost ratio of milk per litre was higher (1:1.33) in cross-bred cows than native ones (1:1.04). The study further showed that in comparing with bulk line cost, the price of milk per litre received by the farmers was higher in cross-bred cows than native cows. Therefore, the study recommends dairying with cross-bred cows as encouraging and viable commercial enterprise in Banfladesh.

• 한국농공학회지
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• 제15권4호
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• pp.3170-3180
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• 1973

This study of the subject will review past and present irrigation development in Korea. Particular attention will be given to water pricing structure and a case study on the purpose of rational operation and management of irrigation water and organizations, and the optimum irrigation water and organizations, and the optimum irrigation water fee inorder to reduce farmers burden and to rationalize the farmland associations management so as to achieve development of the rural environment. In 1971, the reservoir of the Farmland Improvement A sociation (FIA) produced only 775 millison $m^3$ of irrigation water or 77% of planned capacity of 1,015 million $m^3$. It was caused by inefficient maintenance of irrigation facilities; for instance, about 21% of reservoirs, pumping stations and weirs in Korea have been silted by soil erosion which hinder to water production according to an ADC survey. The first Irritation Association was established in 1906, whcih was renamed the Farmland Assoeiation by the Rural Development Enouragement Law in 1970. By the end of 1971, 411,000 ha of rice paddies were under the control of 267 associations nationwide. The average water price assessed by Associations nationwide rose from 790 won per 0.1 ha. in 1966 to 1,886 won in 1971. The annual growth rate was 20%. The highest water price in 1971 was 4,773 won her 0.1 ha. and the lowest was 437 won. This range was caused by differences in debt burden, geographic conditions and management efficiency among the Associations. In 1971, the number of Associations which exceeded the average water price of 1,886 won per 0.1 ha. was 144, or 55.1% of all Association. In determination of water price, there are two principles; one is determined by production cost such as installation cost of irrigation facilities, maintenance cost, management cost and depreciation ect. For instance, the Yong San River Development project was required 33.7 billion won for total construction and maintenance cost is 3.1 billion won for repayment, maintenance and management cost per year. The project produces 590 million $m^3$ of irrigation water annually. Accordingly, the water price per $m^3$ is 5.25 won. The other principle is determined by water value in the crop products and in compared with production of irrigated paddy and non-irrigated paddy. By using this method, water value in compared with paddy rice vs. upland rice(Average of 1967-1971) was 14.15 won per $m^3$ and irrigated paddy vs. non-irrigated paddy was 2.98 won per $m^3$. In contrast the irrigation fee in average association of 1967-1971 was 1.54 won per $m^3$. Accordingly, the current national average irrigation fee(water price) is resonable compared with its water value. In this study, it is found that the ceiling of water price in terms of water value is 2.98 won per $m^3$ or 2,530 won per 0.1 ha. However, in 1971 55% of the associations were above the average of nationwide irrigation fees. which shows the need for rationalization of the Association's management. In connection with rationalization of the Association's management, this study recommends the following matters. (1) Irrigation fee must be assessed according to the amount of water consumption taking intoaccount the farmer's ability. (2) Irrigation fee should be graded according to behefits and crop patterns. (3) Training personnel in the operation and procedures of water management to save O&M costs. (4) Insolvent farmland association should be integrated into larger, sound associations in the same GUN in order to reduce farmers' water cost. (5) The maintenance and repair of existing irrigation facilities is as important as expansion of facilities. (6) Establishment of a new Union of Farmland Association is required to promoted proper maintenance and to protect the huge investment in irrigation facilities by means of technical supervision and guidance.

• 한국작물학회지
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• 제40권1호
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• pp.86-91
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• 1995

벼 생력 직파재배양식으로 최근에 개발 보급된 무논골뿌림재배의 파종 피술체계확립의 일환으로 파종량에 따른 생육 및 수량반응을 검토하고자 시험을 실시하였던바, 그 결과를 요약하면 다음과 같다. 1. 파종량이 증가할수록 $m^2$당 입모수는 직선적으로 증가하였으나, 개체당 수수는 입모수 증가에 따라 완만하게 감소하는 경향이었다. 2. 유효경 비율 및 출수기 경당엽면적은 파종량이 증가함에따라 감소하였으며 출수기는 큰차이가 없었다. 3. 도복관련 형질중 간장, 수장, 좌절량, 줄기굵기 등이 파종량 증가와 함께 감소하는 경향이었고, 10a당 7kg이상에서는 포장도복이 심하게 발생되었다. 4. $m^2$당의 수수증가에 따라 수당립수와 등숙비율이 감소하였으며, 수량은 10a당 5kg을 기점으로하여 점차 감소하는 경향이었다. 5. 수량에 대한 수량구성요소의 경로계수 분석결과에 의하면 등숙비율의 직접효리가 가장컸으며, 최대수량을 얻기위한 $m^2$당 입모수는 105개 정도, 이때의 10a당 파종량은 4.5kg 수준이었다.