• Biomedical Science Letters
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• v.11 no.4
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• pp.493-501
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• 2005

Circadian rhythms, time on a scale of about 24 hours, are present in a number of organisms including animals, plants, and bacteria. The control of the biochemical, physiological and behavioral processes is regulated by endogenous clocks in the suprachiasmatic nucleus (SCN). At the core of this timing mechanism is molecular machinery that are present both in the brain and in the peripheral tissues throughout the body, and even in a single cultured cell. In this study, we performed two-dimensional gel electrophoresis to figure out any correlation between protein expression patterns and the requirement of two canonical clock proteins, either mPER1 or mPER2, by comparing global protein expression profiles in livers from wildtype or mPer1/mPer2 double mutant mice. We could identify several differentially expressed protein candidates with respect to time and genotypes. Further analysis of these candidate proteins in detail in vivo will lead us to the better understanding of how circadian clock functions in mammals.

• Journal of Animal Environmental Science
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• v.5 no.3
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• pp.149-156
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• 1999

The experiment was conducted to determine the optimum stocking density for Korean Native Chicken. The experiment was carried out with 810 Korean Native Chickens for 16weeks from April 22. 1987 to August 11. 1987. The chickens were housed in pens with varying stocking densities; T1(20 birds per 3.3$m^2$), T2(30 birds per 3.3$m^2$), T3(40 birds per 3.3$m^2$), T4(50 birds per 3.3$m^2$), T5(60 birds per 3.3$m^2$) and T6(70 birds per 3.3$m^2$). Each treatment contained three replicates. At the end of the trial, the average body weight of T1 was significantly heavier than that of T5 (P<0.05), but there were no statistical differences among the treatments in fed intake. The feed conversion of T3 was improved significantly in comparison with that of T5(P<0.05), and the viability of T1 showed a significantly difference with that of T5, T1 showed the highest production number, whereas T5 the lowest one.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.2
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• pp.57-63
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• 1979

This study has been carried out to find out the optimum plant density and number of seedling per hill in the late season culture of rice at Chung Buk. P.O.R.D. Up to the level of 33 hills per m2 and 6 number of seedling per hill, rice yield was increased as the number of seedling per hill and plant density per unit areas increased. However it is recommendable to have a plant density of 28~33 hills per m$^2$ with 4~6 number of seedling per hill for the late season culture.

• BMB Reports
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• v.28 no.5
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• pp.375-381
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• 1995

Two forms of glucoamylase (GI and GII) from starch-grown Lipomyces kononenkoae CBS 5608 mutant were purified to apparent homogeneity by means of ultrafiltration, Sephacryl S-200 gel filtration and DEAE Sephadex A-50 chromatography. The apparent molecular weight was calculated as ca. 150 kDa for GI and ca. 128 kDa for GII, respectively. Both enzymes were glycoproteins with isoelectric points of 5.6 (GI) and 5.4 (GII). They had a pH optimun of 4.5 and were stable from pH 5 to 8. The temperature optimum for both enzymes was $60^{\circ}C$, but they were rapidly inactivated above $70^{\circ}C$. The $K_m$ values toward starch were estimated to be 6.57 mg per ml for GI and 4.52 mg per ml for GII, and the $V_{max}$ values were 16.28 ${\mu}M$ per mg for GI and 32.25 ${\mu}M$ per mg for GII, respectively. The $K_m$ and $V_{max}$ values of GII for ${\alpha}-$ or ${\beta}-cyclodextrin$ were estimated to be 0.15 mg per ml and 2.0 mg per ml, respectively ($K_m$) and 1.02 ${\mu}M$ per mg or 1.02 ${\mu}M$ per mg, respectively ($V_{max}$). Neither enzyme exhibited pullulanase activity but they released only glucose from starch or cyclodextrin. Amino acid analysis indicated that both glucoamylases were enriched in proline and acid amino acids. Glucoamylase GII strongly cross-reacted with a monoclonal antibody raised against GI enzymes, and the two enzymes shared very similar amino acid composition. Western blot analysis indicated that L. kononenkoae CBS 5608 mutant produced two forms of glucoamylase on starch, and that synthesis of them was subject to glucose repression.

• Journal of Cadastre & Land InformatiX
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• v.45 no.2
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• pp.217-235
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• 2015

The study has analyzed the determinants of retail rents for the franchised coffee shop with the Multi-Regression analysis in Seoul. Central place, agglomeration, and demand-externality theories provide the basis for empirical models. The data used in this study consisted of 2,000 retail units in Seoul. As the results, the results show that coffee shop of retail sales per $3.3m^2$ and characteristic of retail trade-areas, and characteristic of coffee shop affects on the coffee shop's rents per $3.3m^2$. Coffee Shop's sales are the major determinant of coffee shop's rents. Also, coffee shop's goodwill per $3.3m^2$ and rental bond per $3.3m^2$ are positively correlated with a coffee shop's rents. Additionally, number of wholesale company and manufacturers in the retail trade-areas influences coffee shop's rent per $3.3m^2$. In summary, the study shows that coffee shop's brand identity, location and retail mix in the retail trade areas affects a coffee shop's rent per $3.3m^2$.

• Journal of the Korean housing association
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• v.15 no.5
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• pp.51-58
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• 2004

After 1980's, the ratio of car possessions has been increased rapidly. Nowaday, most large cities have suffered a lot of problems about traffic and car parking. Specially in apartment complexes, they have parking problem which is caused by the parking demand. The ratio of parking demand at multi-family housing sites has been increased significantly, therefore several parking problems have occurred. The goal of this study is to investigate the parking demand per housing unit size of apartment complex in Daegu city. The results of this study is as follows. (1) The parking demand of 60 $m^2$ below sized housing unit is 1.09 car per the unit. (2) The parking demand of 60 $m^2$ over 85 $m^2$ below sized housing unit is 1.31 car per the unit. (3) The parking demand of 85 $m^2$ over 135 $m^2$ below sized housing unit is 1.74 car per the unit. (4) The parking demand of 135 $m^2$ over sized housing unit is 2.10 car per the unit.

• Journal of the Korean Society of Radiology
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• v.11 no.5
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• pp.321-328
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• 2017

The purpose of the study is to provide basic data for the management of individual exposure and the monitoring of natural radiation dose using D-Shuttle dosimeter (Chiyoda Technol Corporation, Tokyo, Japan). The dose was calculated using D-Shuttle dosimeter. The dose was 1.346 mSv when exposed for 400 days, the annual dose per year was 1.228 mSv/year and the average dose per hour was $0.014{\mu}Sv/hr$. Domestic individual external dose (1.295 mSv/year = Korea average natural individual external dose) and domestic additional dose per year is -0.0663 mSv/year. D-Shuttle is a personal dosimeter for radiation monitoring. It can be used as a very useful dosimeter for ALARA because of its excellent detection capability of radiation, real-time radiation exposure management, alarm function of radiation work, and efficient and easy to use personal radiation dose management.. Radiation monitoring equipment for radiation workers and local residents can be used for radiation monitoring in hospitals, industry, medical sites, nuclear accident areas and hazardous areas in non-destructive areas.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.1-10
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• 2015

This study was conducted in three years (7-9 years after planting) to investigate vegetative growth, yield, fruit quality, and return bloom for optimum crop load based on different planting densities of adult 'Fuji'/M.9 apple trees. As plant materials, 'Fuji'/M.9 apple trees planted at $3.5{\times}1.5m$ (190 trees per 10 a), $3.5{\times}1.2m$ (238 trees per 10 a), and $3.2{\times}1.2m$ (260 trees per 10 a) spacing and trained as slender spindles were used. The crop load was assigned to five different object ranges as follows: 55-64, 65-74, 75-84, 85-94, and 95-104 fruit per tree. TCA increment, total shoot growth, return bloom, yield per tree, and yield efficiency tended to increase as planting density decreased, and fruit weight and soluble solid content tended to increase as the object range of crop load decreased. Fruit red color tended to increase as shoot growth decreased. For apple trees planted with 238 trees and 260 trees per 10a, biennial bearing occurred when the crop load was over 85-94 and 75-84 fruits, respectively. However, biennial bearing did not occur when the crop load was 95-104 fruits in apple trees planted with 190 trees per 10a. Accumulated yield tended to increase as planting density and crop load increased, but that of biennial bearing did not show such a difference. Based on our results, optimum crop load recommendations are to set 95-104 fruits per tree in 'Fuji'/M.9 mature apple trees planted at 190 trees per 10a, 75-84 fruits per tree at 238 trees per 10a, and 65-74 fruits per tree at 260 trees per 10a.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.3
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• pp.234-243
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• 2000

This paper is to study population ecological characteristics, including growth parameters, survival rate, instantaneous coefficients of natural and fishing mortalities, and age at first capture of the soft-shelled clam, Mya japonioa in the intertidal zone of South Sea in Korea. For describing growth of the clam a von Bertalanffy growth model was adopted, The von Bertalanffy growth curve had an additive error structure and the growth parameters estimated from a non-linear regression were SH/sub ∞/=79.83mm, K=0.26, and t/sub 0/= -0.01. Survival rate (S) of the soft-shelled clam was 0.26 (SD=0.02). The instantaneous coefficients of natural mortality (M) was estimated to be 0.78/year and fishing mortality (F) 0.57/year for the soft-shelled clam. The age at first capture (t/sub c/) was estimated as 2.69 year. The mean densities of the soft-shelled clam by bottom type were 3.40 inds./m²(SE=0.18) in the sand, 63.4 inds./m²(SE= 0.53) in the muddy sand, and 0 inds./m2 (SE=0) in the gravelly sand. The mean densities of the soft-shelled clam by 3 different areas were 4.88 inds./m²(SE=0.09), 2.61 inds./m²(SE=0.13), 7.20 inds./m²(SE=0.18), respectively and the biomass of the clam were estimated as 131mt, 121mt, 665mt, respectively. An yield-per-recruit analysis showed that the current yield-per-recruit of about 8.30g with F=0.57/year and the age at first capture (t/sub c/) 2.69 year, was lower than the maximum possible yield-per-recruit of 9.60g. Fixing to at the current level and increased fishing intensity (F) could produce an increase in the predicted yield-per-recruit from 8.30g to about 9.40. However, estimated yield-per-recruit increased to 1.30g by decreasing to from the current age (2.69 year) to age two with F fixed at the current level. Yield-per-recruit was estimated under harvest strategies based on F/sub max/ and F/sub 0.1/.

• Journal of Magnetics
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• v.4 no.3
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• pp.76-79
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• 1999

We report results on metastable CuCo ribbons at low Co contents (2 and 10 at %), which were prepared by conventional melt-spinning technique and subsequent annealing. The properties of these materials cannot consistently be described by those of an assembly of superparamagnetic single-domain particles. Magnetic measurements related to magnetic dynamics reveal spin-glass-like properties. Especially, we find very slow nonequilibrium relaxation processes in Co10Cu90, which depend on prehistory, when probing the relaxation of the resistivity. The results are clear evidence for frustrated interaction effects due to magnetic couplings between Co clusters or precipitates in these alloys.