• Toxicological Research
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• 제29권2호
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• pp.115-120
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• 2013

To investigate the effects of short-term exposure of beryllium on the human immune system, the proportion of T-lymphocytes such as CD3+, CD4+, CD8+, CD95, and NK cells, and the proportion of B cells and $TNF{\alpha}$ level in peripheral blood and immunoglobulins in the serum of 43 exposed workers and 34 healthy control subjects were studied. External exposure to beryllium was measured by atomic absorption spectrometer as recommended by the NIOSH analytical method 7300. T lymphocyte subpopulation analysis was carried out with flow cytometer. The working duration of exposed workers was less than 3 months and the mean ambient beryllium level was $3.4{\mu}g/m^3$, $112.3{\mu}g/m^3$, and $2.3{\mu}g/m^3$ in molding (furnace), deforming (grinding), and sorting processes, respectively (cited from Kim et al., 2008). However, ambient beryllium level after process change was non-detectable (< $0.1{\mu}g/m^3$). The number of T lymphocytes and the amount of immunoglobulins in the beryllium-exposed workers and control subjects were not significantly different, except for the total number of lymphocytes and CD95 (APO1/FAS). The total number of lymphocytes was higher in the beryllium-exposed individuals than in the healthy control subjects. Multiple logistic regression analysis showed lymphocytes to be affected by beryllium exposure (odd ratio = 7.293; p<0.001). These results show that short-term exposure to beryllium does not induce immune dysfunction but is probably associated with lymphocytes proliferation.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2011년도 춘계학술대회 초록집
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• pp.201.2-201.2
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• 2011

A water-source heat pump system has been developed for cooling and heating of a green house on the waterfront in Jinju. In order to supply a heat source/sink of water in alluvium aquifer to the heat pump system, the riverbank filtration facility (two pumping wells and one recharge well) for water intake and injection has been constructed. To pump and recharge water sufficiently, the geometric design such as depth and diameter for the wells have been completed, and details of the well such as slot size and length of the screen and filter pack size have been designed based on the practical and theoretical design method including D30 technique. For the investigation of the hydrogeological characteristics, step-drawdown test, long-term pumping test, and recovery test have been carried out for two developed pumping wells. Step-drawdown test has been performed on 4 step flowrates of 150, 300, 450, $600m^3$/day for 1 hour, and long-term pumping test on flowrate of $500m^3$/day for 24 hours, and recovery test for 6 hours. Since the underground water filtrated by riverbank is flowing smoothly into the well, the water level goes down slightly for the long-term test. Consequently, the stable pumping flowrate for two pumping well has been predicted at least over $1,647m^3$/day which is larger than the flowrate of $1,000m^3$/day for a 60 RT heat pump system.

• The Korean Journal of Physiology and Pharmacology
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• 제8권6호
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• pp.295-300
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• 2004

Serotonin (5-hydroxytroptamine, 5-HT) has been shown to affect the induction of long-term potentiation (LTP) in the cortex such as the hippocampus, the visual cortex and the prefrontal cortex. Fluoxetine, as a selective serotonin reuptake inhibitor, is used in the management of a wide variety of psychological diseases. To study the effect of fluoxetine on the induction of LTP, we recorded the field potential in layer II/III of the frontal cortex from 3-wk-old. LTP was induced in horizontal input by theta burst stimulation (TBS). TBS with two-folds intensity of the test stimulation induced LTP, which was blocked by application of D-AP5 $(50 {\mu}M)$, an NMDA receptor antagonist. Whereas bath application of 5-HT $(10 {\mu}M)$ inhibited the induction of LTP, treatment with the 5-HT depleting agent, para-chloroamphetamine $(PCA,\;10{\mu}M)$, for 2hr did not affect the induction of LTP. Bath application of fluoxetine (1, 3, and $10 {\mu}M)$ suppressed the induction of LTP in concentration-dependent manner, however, fluoxetine did not inhibit the induction of LTP in 5-HT-depleted slices. These results indicate that fluoxetine may inhibit the induction of LTP by modulating serotonergic mechanism in the rat frontal cortex.

• 한국도로학회논문집
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• 제7권4호
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• pp.69-77
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• 2005

줄눈 콘크리트포장에서 적정한 줄눈간격은 슬래브의 거동에 의해 발생하는 인장응력 줄눈채움재의 파손과 다웰바의 하중전달율(LTE)의 저하를 방지하여 도로포장의 장기 공용성 증가에 큰 영향을 미친다. 하지만 국내 줄눈간격은 경험적이고 획일적으로 이루어진다. 그러므로 본 연구는 국내 권역별 기후조건을 토대로 합리적인 줄눈간격 산정에 대한 방안을 제시하였다. 합리적인 줄눈간격은 초기거동에서 환경하중의 영향에 따른 건조수축과 수화열에 의해 무작위 균열 발생하지 않도록 산정하였다. 그리고 장기거동에서 줄눈폭의 과도한 움직임에 따른 LTE 저하로 인해 포장파손이 발생하지 않도록 줄눈간격을 산정하였다. 본 연구에서 산정된 줄눈간격 잠정안은 6$\sim$8m이며, 포장파손 예측 모델을 통해 줄눈간격을 8m로 증가시켜도 포장 공용성엔 큰 차이가 없었다.

• 대한토목학회논문집
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• 제32권6A호
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• pp.389-398
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• 2012

이 연구에서는 빠른 조류 환경에서의 재킷식 해양구조물인 울돌목 시험조류발전소 구조물에 대한 시공 중 및 운영 중 변형률을 장기 계측하여, 계측 자료를 다양하게 분석하였다. 우선 구조물 시공 단계별 변형률 응답의 변화를 분석하여, 양중, 블록재하, 파일 인발 및 근입 등의 작업 시 변형률에 큰 변화가 발생함을 제시하였다. 또한 재킷 레그 절단과 같은 구조변경 후의 고정하중 및 조류하중에 의한 변형률 변화를 분석하여, 조류하중보다는 고정하중에 의한 변형률의 변화가 크게 발생함을 확인하였으며, 운영 중 계측 결과로부터 주요 변동 주기 성분을 분석하여, 변형률 계측 자료에 M2 및 M4 조석 성분과 동일한 주기를 갖는 장주기 성분이 가장 크게 존재함을 확인하였다. 마지막으로 변형률 기반의 구조 건전성 모니터링에 있어 중요한 변형률 예측 모델을 신경망 기반으로 작성하여, 조류 유속 및 조위에 따른 변형률을 합리적인 수준에서 예측할 수 있음을 확인하였다.

• Animal Bioscience
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• 제36권12호
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• pp.1889-1897
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• 2023

Objective: 'Cultured meat' has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained in vitro over long periods. Fibronectin is a glycoprotein that affects biological processes such as cell adhesion, differentiation, and migration. Unfortunately, the characteristics of porcine satellite cells grown in a long-term culture when exposed to fibronectin-coated dishes are unknown. The objective of this study was to investigate the appropriate concentration of fibronectin coated dishes for proliferation and maintenance of porcine satellite cells at long-term culture. Methods: In this study, we isolated the satellite cells and fibroblast cells with pre-plating method. We next analyzed the cell doubling time, cell cycle, and rate of expressed paired box 7 (Pax7) and myogenic differentiation 1 (MyoD1) in porcine satellite cells cultured with 20 ㎍/mL of fibronectin-, gelatin-, and non-coated dishes at early and late passage. We then analyzed the proliferation of porcine satellite cells with various concentrations of mixed gelatin/fibronectin. We next determined the optimal concentration of fibronectin that would encourage proliferation and maintenance of porcine satellite cells in a long-term culture. Results: Doubling time was lowest when 20 ㎍/mL of fibronectin was used (as tested during an early and late passage). Levels of expressed Pax7 and MyoD1, assessed using immunocytochemistry, were highest in cells grown using fibronectin-coated dishes. The proliferation of gelatin/fibronectin mixed coatings had no significant effect on porcine satellite cells. The concentration of 5 ㎍/mL fibronectin coated dishes showed the lowest doubling time and maintained expression of Pax7. Conclusion: Fibronectin with 5㎍/mL effectively maintains porcine satellite cells, a discovery that will be of interest to those developing the next generation of artificial meats.

• 동아시아식생활학회지
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• 제22권5호
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• pp.657-666
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• 2012

The purpose of this study was to improve the quality of Korean traditional sauce products by producing short-term green gochujang with cheongyang pepper powder. To determine its antioxidant compounds and activities, we examined vitamin C, capsaicinoid, total phenolic, and total flavonoid contents, as well as electron-donating, SOD-like, ferrous ion-chelating, and nitrite-scavenging activities. Vitamin C content of short-term green gochujang was higher in CON-M than in the sample, whereas capsaicinoid content increased as the amount of cheongyang pepper powder increased. Total phenolic content, total flavonoid content, electron-donating activity, and nitrite-scavenging activity were higher in the sample than in CON-M, and the contents increased as the amount of meju powder increased. Green gochujang containing the highest amount of cheongyang pepper powder showed the highest SOD-like, and ferrous-ion chelating activities as well as superior nutrient contents, compared to red gochujang.

• 한국태양에너지학회 논문집
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• 제32권6호
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• pp.37-43
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• 2012

Long-term variations in wind speed and capacity factor(CF) on Seongsan wind farm of Jeju Island, South Korea were derived statistically. The selected areas for this study were Subji, having a year wind data at 30m above ground level, Sinsan, having 30-year wind data at 10m above ground level and Seongsan wind farm, where long-term CF was predicted. The Measure-Correlate-Predict module of WindPRO was used to predict long-tem wind characteristics at Seongsan wind farm. Eachyear's CF was derived from the estimated 30-year time series wind data by running WAsP module. As a result, for the 30-year CFs, Seongsan wind farm was estimated to have 8.3% for the coefficien to fvariation, CV, and-16.5% ~ 13.2% for the range of variation, RV. It was predicted that the annual CF at Seongsan wind farm varied within about ${\pm}4%$.

• 한국해양공학회지
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• 제21권4호
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• pp.15-20
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• 2007

The harbor siltation by longshore sediment transports has become a serious problem on the East Coast of Korea. A reasonable prediction of the longshore sediment rate is important to approach the siltation problem effectively. In the recently developed 1-line model, the empirical constants of the sediment transport formula, which include the absolute quantity of sediment transport rate and the spatial distribution of breaking wave height by wave deformation, are treated as calibration parameters. Since these constants should be determined by the very long-term shoreline data, the longshore sediment rates are much more reasonable values. The method was applied to Hupo Beach, which has experienced heavy siltation. The authors also discuss long-term shoreline change using aerial photos and the observed wave-induced current patterns. According to the result, the SW-direction sediment transport rate was $146,892m^3/year$, and the NE direction was $2,694,450m^3/year$ at Hupo Beach for the last 11 years. The siltation in Hupo Harbor might be affected by the NE-direction sediment transport from Hupo Beach.

• Korea-Australia Rheology Journal
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• 제18권4호
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• pp.199-207
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• 2006

Measurements by Luap et al. (2005) of elongational viscosity and birefringence of two nearly monodisperse polystyrene melts with molar masses $M_{w}$ of $206,000g{\cdot}mol^{-1}$ (PS206k) and $465,000g{\cdot}mol^{-1}$ (PS465k) respectively are reconsidered. At higher elongational stresses, the samples showed clearly deviations from the stress optical rule (SOR). The elongational viscosity data of both melts can be modeled quantitatively by the MSF model of Wagner et al. (2005), which is based on the assumption of a strain-dependent tube diameter and the interchain pressure term of Marrucci and Ianniruberto (2004). The only nonlinear parameter of the model, the tube diameter relaxation time, scales with $M_{w}^{2}$. In order to get agreement with the birefringence data, finite chain extensibility effects are taken into account by use of the $Pad\'{e}$ approximation of the inverse Langevin function, and the interchain pressure term is modified accordingly. Due to a selfregulating limitation of chain stretch by the FENE interchain pressure term, the transient elongational viscosity shows a small dependence on finite extensibility only, while the predicted steady-state elongational viscosity is not affected by non-Gaussian effects in agreement with experimental evidence. However, deviations from the SOR are described quantitatively by the MSF model by taking into account finite chain extensibility, and within the experimental window investigated, deviations from the SOR are predicted to be strain rate, temperature, and molar mass independent for the two nearly monodisperse polystyrene melts in good agreement with experimental data.