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Norepinephrine induces MAIL mRNA expression in primary cultured hepatocytes through IL-1β released from non-parenchymal cells

  • Kim, Hyeon-Cheol;Jung, Bae Dong
    • Korean Journal of Veterinary Research
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    • v.50 no.2
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    • pp.79-84
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    • 2010
  • The molecule possessing ankyrin-repeats induced by lipopolysaccharide (MAIL) protein is a novel member of the $Ikappa{\beta}$ family. In the present study, we examined the effect of norepinephrine (NE) on MAIL mRNA expression in primary cultured mouse hepatocytes and non-parenchymal liver cells. MAIL mRNA expression in hepatocytes and non-parenchymal liver cells was not directly influenced by NE. However, MAIL mRNA expression in hepatocytes was significantly induced by incubation with a culture medium of non-parenchymal liver cells, treated with NE. Pretreatment with an interleukin (IL)-1 receptor antagonist significantly attenuated the stimulatory effect of the medium. Moreover, exogenous $IL-1{\beta}$ induced MAIL mRNA expression in hepatocytes, while IL-6 and tumor necrosis factor $\alpha$ did not. The concentration of $IL-1{\beta}$ in the medium of non-parenchymal liver cells was significantly increased after NE-treatment. These results suggest that NE can induce MAIL mRNA expression in hepatocytes through $IL-1{\beta}$, released from non-parenchymal liver cells.

A Study on the Parking Demand of Apartment Complex in Daegu city (대구시 아파트단지의 주차수요에 관한 연구)

  • Park Chan-Don;Ha Jae-Myung
    • Journal of the Korean housing association
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    • v.15 no.5
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    • pp.51-58
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    • 2004
  • After 1980's, the ratio of car possessions has been increased rapidly. Nowaday, most large cities have suffered a lot of problems about traffic and car parking. Specially in apartment complexes, they have parking problem which is caused by the parking demand. The ratio of parking demand at multi-family housing sites has been increased significantly, therefore several parking problems have occurred. The goal of this study is to investigate the parking demand per housing unit size of apartment complex in Daegu city. The results of this study is as follows. (1) The parking demand of 60 $m^2$ below sized housing unit is 1.09 car per the unit. (2) The parking demand of 60 $m^2$ over 85 $m^2$ below sized housing unit is 1.31 car per the unit. (3) The parking demand of 85 $m^2$ over 135 $m^2$ below sized housing unit is 1.74 car per the unit. (4) The parking demand of 135 $m^2$ over sized housing unit is 2.10 car per the unit.

A Study on the Support and Infill System of Flexible Housing Units in Apartment Houses for Meeting the Residents' Variety - Focused on 126.6m$^2$-Sized Apartment in Busan - (거주자의 다양성 수용을 위한 가변형 공동주택의 Support와 Infill 시스템에 관한 연구 - 부산시 전용면적 126.6 m$^2$ 아파트를 중심으로 -)

  • Kim, Min-Kyoung;Oh, Chan-Ohk
    • Proceeding of Spring/Autumn Annual Conference of KHA
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    • 2005.11a
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    • pp.171-175
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    • 2005
  • The purpose of this study was to suggest the Support and Infill system for 126.6m$^2$-sized housing unit which meets residents' variety. The subjects were 100 housewives who lived in 126.6m$^2$-sized housing unit in the apartment complex, Busan. The data was collected by using model and questionnaire. The model was made to be same as the current 126.6m$^2$-sized floor plan. The residents preferred the flexible housing unit apartment complex. Also, the residents' needs for floor plan of housing unit were various. Therefore, the development of flexible housing unit is needed. On the basis of these findings, the support and infill system for 126.6m$^2$-sized housing unit was suggested.

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Relationship between University students' Marriage View and Quality of Life

  • Kim, Jungae;Cho, Euiyoung
    • International Journal of Advanced Culture Technology
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    • v.8 no.4
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    • pp.129-136
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    • 2020
  • This study was a cross-sectional descriptive study that analyzed the effect of quality of life on marriage views in college students. The purpose of this study was to identify the relationship between quality of life and marriage view. The study targets were 195 male and female university students aged between 20 and 25. The data collection was done only by those who voluntarily agreed to the study using online questionnaires, and the data collection period was from October 1 to October 30, 2020. Data analysis was performed using SPSS 18.0 for frequency analysis, χ2 analysis, Pearson correction, t-test, and simple regression analysis. The result of the study that men had higher conservative marriage view (M=3.22, SD=.716) than women (M=2.21, SD=.903, p<0.01), women had higher active marriages (M=3.94, SD=.955) than men (M=3.41, SD=.933, p<0.01), and women had higher exclusive marriage views (M=3.94, SD=.955) than men (M=3.41, SD=.933, p<0.01). And people with high quality of life positively influenced romantic marriage view (R2=.029, p<0.05), but negatively affected passive marriage view (R2=.029, p<0.05) and conservative marriage view (R2=.034, p<0.05). Based on the results of this study, a program is needed to create a positive marriage view for the married age group, create an environment for married people to positively engage in social and economic activities, and propose a program for creating healthy family relationships.

Control Variables of Remote Joint Analysis Realization on the M2M Case

  • Lim, Sung-Ryel;Choi, Bo-Yun;Lee, Hong-Chul
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.6 no.1
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    • pp.98-115
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    • 2012
  • New trend called ubiquitous leads the recent business by standardization and integration. It should be the main issue how to guarantee the integration and accountability on each business, especially in mission critical system which is mainly supported by M2M (Machine to Machine) control mechanism. This study is from the analysis of digital forensics case study that is from the M2M Sensing Control Mechanism problem of the "Imjin River" case in 2009, where a group of family is swept away to death by water due to M2M control error. The ubiquitous surroundings bring the changes in the field of criminal investigation to real time controls such as M2M systems. The needs of digital forensics on M2M control are increasing on every crime scene but we suffer from the lack of control metrics to get this done efficiently. The court asks for more accurately analyzed results accounting high quality product development design. Investigators in the crime scene need real-time analysis against the crime caused by poor quality of mission critical systems. It seems to be every need of Real-Time-Enterprise, so called ubiquitous society on the case. We try to find the efficiency and productivity in discovering non-functional design defects in M2M convergence products focusing on three metrics in study model with quick implementation. Digital forensics system in present status depends on know-how of each investigator and is hard to expect professional analysis on every field. This study set up a hypothesis "Co-working of professional investigators on each field will qualify Performance and Integrity" especially in mission critical system such as M2M and suggests "Online co-work analysis model" to efficiently detect and prevent mission critical errors in advance. At the conclusion, this study proved the statistical research that was surveyed by digital forensics specialists around M2M crime scene cases with quick implementation of dash board.

Expression of Proapoptotic Bcl-2 Family Member in the Mouse Ovary (I) (생쥐 난소에서 Bcl-2계 세포고사인자에 관한 연구 (I))

  • Lee, Yu-Il;Lee, Jin;Chun, Sang-Young
    • Clinical and Experimental Reproductive Medicine
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    • v.30 no.1
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    • pp.47-55
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    • 2003
  • Objectives: Bok, Bcl-2-related ovarian killer, is a proapoptotic Bcl-2 family protein identified in the ovary based on its dimerization with the antiapoptotic protein Mcl-1. The present study examined the hormonal regulation and localization of Bok messenger RNA levels in the mouse ovary during the follicle development. Methods: The animals were implanted subcutaneously with Silastic brand capsules containing the synthetic estrogen, DES at $21{\sim}23$ days of age. Ovaries were collected $1{\sim}3$ days after implantation for RNA analysis and in situ hybridization. Some mice were removed capsule for $1{\sim}2$ days to induce ovarian follicle apoptosis. Ovaries were also collected from 26 day-old immature mice at various times after treatment with 10 IU PMSG. Some mice received a single intraperitoneal injection of 10 IU hCG to induce ovulation, and ovaries were obtained at different time intervals for Northern blot and in situ hybridization analysis, respectively. Results: Treatment of immature mice with diethylstilbestrol (DES) for $24{\sim}48$ h increased ovarian Bok mRNA levels. Bok mRNA was remained the same levels in mice removed DES for $24{\sim}48$ h to induce apoptosis. High signals of Bok mRNA after DES treatment were detected in granulosa cells of early antral follicles. Treatment of immature mice with PMSG for 12 h increased markedly ovarian Bok mRNA expression which was detected mainly in preantral and atretic follicles. Interestingly, low levels of Bok mRNA were also expressed in granulosa cells of preovulatory follicles. Treatment of PMSGprimed mice with hCG stimulated strongly ovarian Bok mRNA expression at $6{\sim}9$ h. At that time, Bok mRNA was expressed in granulosa cells of atretic and small growing follicles. Conclusion: These results demonstrate that Bok is one of proapoptotic Bcl-2 members expressed in early growing and atretic follicles during the ovarian follicular development. Gonadotropins induce a transient increase of Bok gene expression in granulosa cells of preantral and preovulatory follicles indicating some role in the ovulatory process.

A Study on the Habitat Environment Survey and Conservation of Rhododendron micranthum in National Baekdudaegan Arboretum (국립백두대간수목원 꼬리진달래 자생지 환경특성 및 보전방안)

  • Chung, Bo-Kwang;Gang, Sin-Gu;Bae, Jun-Gyu;Kim, Jae-Hyeon;Lee, Juyoung;Chang, Jeong-Won;Lee, Sang-Hyun;Lee, Yeong-Su;An, Min-Woo;Kim, Gi-Song
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.19 no.6
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    • pp.87-100
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    • 2016
  • This research was performed for the purpose of providing useful and base data and preservation strategy of growth characteristics of Rhododendron micranthum. From the result of research, wild growth area of Rhododendron micranthum was discovered near steep forest road on 695~901m in altitude above sea level of Baekdu-daegan National Arboretum. Vegetation colony structure of Site I was resulted in Pinus densiflora(tree stratum M.I.P.=45.3%) is dominants, Betula schmidtii(arborescent M.I.P.=9.8%) is second dominant, and Site III resulted in Pinus densiflora(tree stratum M.I.P.=30.5%) is dominants, Betula schmidtii(arborescent M.I.P.=10.3%) is second dominant. Site II resulted in Betula schmidtii(arborescent M.I.P.=30.4%) is dominants and Pinus densiflora tree(tree stratum M.I.P.=8.3%) is second dominants. For herbs, it was researched that the rate of one year herb of chrysanthemum family(10), rice family(3), sedge(2) was higher than near forest area. Among Species diversity index, that of Shannon was in range of 0.7348~1.1090. After comparing this with similar area, this value was relatively low. Dominance analysis result was analyzed that various groups are evenly distributed. Monthly highest average temperature is $23.9^{\circ}$ in Site A and Site B, and monthly lowest average temperature is $-4.5^{\circ}C$ in Site I and $-4.7^{\circ}C$ in Site II. Monthly maximum average humidity is 85.6% in Site I and 83.2% in Site II. Monthly minimum average humidity is 60.9% in Site I and 60.8% in Site II and it showed that these 2 area are located far away but have similar temperature and humidity characteristic and climate environment is similar. It was analyzed that humidity is high with the result of annual average humidity is over 60%. In growth characteristic, it showed that doubt damage of Stephanitis pyrioides, exposure of roots, peeled bark was discovered and supplement on this is urgent.

A STUDY ON THE RELATIONSHIP BETWEEN THE LEVEL OF CAREER AWARENESS AND SELF-ESTEEM OF CHILDREN IN ELEMENTARY SCHOOLS (초등학교 아동들의 진로인식 수준과 자아존중감과의 관계 연구)

  • Kim, Mi-Ran
    • 한국초등상담교육학회:학술대회논문집
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    • 2004.01a
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    • pp.205-222
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    • 2004
  • Fundamental direction of the seventh education curriculum aims to nurture active and creative Koreans for globalized and information-oriented society of 21 century, and emphasizes the importance of career education identifying its subsections that are practices of student-focused education of aptitude, ability and career. Hence, as a clear targeting of career objective from elementary school through relevant career education enables them to recognize appropriate career for themselves, the basis of self- actualization and social contribution must be settled. This paper seeks to identify the relationship between career awareness and self-esteem by examining and analysing differences of sex, region and level. This study selected 536 samples from 4th and 6th year elementary school students in C city and S town of Chungcheong province. Career awareness test and self-esteem test is used as a measurement, statistical process control (SPC) of career awareness and self-esteem (M, SD, r) is made for each variable, and the significance was tested. The result of this research can be summarized as follows: First, generally there is very significant regional differences of career awareness (F=16.817, p<.001), which contains that urban children has higher career awareness (M=120.22) than that of rural children (M=113.87). In subsections of career awareness, self-esteem of urban children is higher than that of rural children, and the attitude & for career and values of girls is higher than that of boys. In terms of the career planning, urban children and 6th year students are higher than rural children and 4th year students. Second, self-esteem generally has very significant regional difference (F=12.123, p<.01), which contains that the self-esteem of urban children (M=106.50) is higher than that of rural children (M=101.80). In comparison of different forms, a very significant difference is as much as p<.01 level (F=11.046), which contains 4th year students (M=105.63) have higher self-esteem than 6th year students (M=102.05). In subsections of self-esteem, gross self-esteem and social self-esteem of urban children are higher than rural children. In terms of family self-esteem, urban children and 4th year students have higher figure than rural children and 6th year students. In school self-esteem, 4th year students had higher level than the 6th. Third, the career awareness and self-esteem are generally related as r= .50, it was very significant at p<. 001 level. All subsections of career awareness had positive relationships except the school self-esteem. According to the result, rural children had relatively low career awareness and self-esteem. This is presumably due to relatively poor circumstances for rural children, comparing to that of urban children. Therefore rural elementary schools should try to let them have appropriate career awareness by associating with family, school and local society to expand the opportunity of experiences for the self perception of aptitude, nature and interest. In addition, we have to strive for the whole-minded education increasing the self-esteem of rural children and giving them positive thinking through career counselling, personal counselling and group counselling.

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The STAR RNA Binding Proteins SAM68, SLM-1 and SLM-2 Interact with Kinesin-I (Kinesin-I과 직접 결합하는 STAR RNA 결합 단백질인 SAM68, SLM-1과 SLM-2의 규명)

  • Seog, Dae-Hyun
    • Journal of Life Science
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    • v.21 no.9
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    • pp.1226-1233
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    • 2011
  • In neurons, kinesin is the molecular motor that transport cargos along microtubules. KIF5s (alias kinesin-I), are heterotetrameric motor conveying cargos, but the mechanism as to how they recognize and bind to a specific cargos has not yet been completely elucidated. To identify the interaction proteins for KIF5C, yeast two-hybrid screening was performed, and specific interaction with the $\underline{S}$am68-$\underline{l}$ike $\underline{m}$ammalian protein $\underline{2}$ (SLM-2), a member of the $\underline{s}$ignal $\underline{t}$ransducers and $\underline{a}$ctivators of $\underline{R}$NA (STAR) family of RNA processing proteins, was found. SLM-2 bound to the carboxyl (C)-terminal region of KIF5C and to other KIF5 members. The C-terminal domain of Sam68, SLM-1, SLM-2 was essential for interaction with KIF5C in the yeast two-hybrid assay. In addition, glutathione S-transferase (GST) pull-downs showed that SAM68, SLM-1, and SLM-2 specifically interacted to Kinesin-I complex. An antibody to SAM68 specifically co-immunoprecipitated SAM68 associated with KIF5s and coprecipitated with a specific set of mRNA. These results suggest that Kinesin-I motor protein transports RNA-associated protein complex in cells.

Problems in the Korean National Family Planning Program (한국가족계획사업(韓國家族計劃事業)의 문제점(問題點))

  • Hong, Jong-Kwan
    • Clinical and Experimental Reproductive Medicine
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    • v.2 no.2
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    • pp.27-36
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    • 1975
  • The success of the family planning program in Korea is reflected in the decrease in the growth rate from 3.0% in 1962 to 2.0% in 1971, and in the decrease in the fertility rate from 43/1,000 in 1960 to 29/1,000 in 1970. However, it would be erroneous to attribute these reductions entirely to the family planning program. Other socio-economic factors, such as the increasing age at marriage and the increasing use of induced abortions, definitely had an impact on the lowered growth and fertility rate. Despite the relative success of the program to data in meeting its goals, there is no room for complacency. Meeting the goal of a further reduction in the population growth rate to 1.3% by 1981 is a much more difficult task than any one faced in the past. Not only must fertility be lowered further, but the size of the target population itself will expand tremendously in the late seventies; due to the post-war baby boom of the 1950's reaching reproductive ages. Furthermore, it is doubtful that the age at marriage will continue to rise as in the past or that the incidence of induced abortion will continue to increase. Consequently, future reductions in fertility will be more dependent on the performance of the national family planning program, with less assistance from these non-program factors. This paper will describe various approaches to help to the solution of these current problems. 1. PRACTICE RATE IN FAMILY PLANNING In 1973, the attitude (approval) and knowledge rates were quite high; 94% and 98% respectively. But a large gap exists between that and the actual practice rate, which is only 3695. Two factors must be considered in attempting to close the KAP-gap. The first is to change social norms, which still favor a larger family, increasing the practice rate cannot be done very quickly. The second point to consider is that the family planning program has not yet reached all the eligible women. A 1973 study determineded that a large portion, 3096 in fact, of all eligible women do not want more children, but are not practicing family planning. Thus, future efforts to help close the KAP-gap must focus attention and services on this important large group of potential acceptors. 2. CONTINUATION RATES Dissatisfaction with the loop and pill has resulted in high discontinuation rates. For example, a 1973 survey revealed that within the first six months initial loop acceptance. nearly 50% were dropouts, and that within the first four months of inital pill acceptance. nearly 50% were dropouts. These discontinuation rates have risen over the past few years. The high rate of discontinuance obviously decreases the contraceptive effectiveness. and has resulted in many unwanted births which is directly related to the increase of induced abortions. In the future, the family planning program must emphasize the improved quality of initial and follow-up services. rather than more quantity, in order to insure higher continuation rates and thus more effective contraceptive protection. 3. INDUCED ABORTION As noted earlier. the use of induced abortions has been increase yearly. For example, in 1960, the average number of abortions was 0.6 abortions per women in the 15-44 age range. By 1970. that had increased to 2 abortions per women. In 1966. 13% of all women between 15-44 had experienced at least one abortion. By 1971, that figure jumped to 28%. In 1973 alone, the total number of abortions was 400,000. Besides the ever incre.sing number of induced abortions, another change has that those who use abortions have shifted since 1965 to include- not. only the middle class, but also rural and low-income women. In the future. in response to the demand for abortion services among rural and low-income w~men, the government must provide and support abortion services for these women as a part of the national family planning program. 4. TARGET SYSTIi:M Since 1962, the nationwide target system has been used to set a target for each method, and the target number of acceptors is then apportioned out to various sub-areas according to the number of eligible couples in each area. Because these targets are set without consideration for demographic factors, particular tastes, prejudices, and previous patterns of acceptance in the area, a high discontinuation rate for all methods and a high wastage rate for the oral pill and condom results. In the future. to alleviate these problems of the methodbased target system. an alternative. such as the weighted-credit system, should be adopted on a nation wide basis. In this system. each contraceptive method is. assigned a specific number of points based upon the couple-years of protection (CYP) provided by the method. and no specific targets for each method are given. 5. INCREASE OF STERILIZA.TION TARGET Two special projects. the hospital-based family planning program and the armed forces program, has greatly contributed to the increasing acceptance in female and male sterilization respectively. From January-September 1974, 28,773 sterilizations were performed. During the same time in 1975, 46,894 were performed; a 63% increase. If this trend continues, by the end of 1975. approximately 70,000 sterilizations will have been performed. Sterilization is a much better method than both the loop and pill, in terms of more effective contraceptive protection and the almost zero dropout rate. In the future, the. family planning program should continue to stress the special programs which make more sterilizations possible. In particular, it should seek to add the laparoscope techniques to facilitate female sterilization acceptance rates. 6. INCREASE NUMBER OF PRIVATE ACCEPTORS Among the current family planning users, approximately 1/3 are in the private sector and thus do not- require government subsidy. The number of private acceptors increases with increasing urbanization and economic growth. To speed this process, the government initiated the special hospital based family planning program which is utilized mostly by the private sector. However, in the future, to further hasten the increase of private acceptors, the government should encourage doctors in private practice to provide family planning services, and provide the contraceptive supplies. This way, those do utilize the private medical system will also be able to receive family planning services and pay for it. Another means of increasing the number of private acceptors, IS to greatly expand the commercial outlets for pills and condoms beyond the existing service points of drugstores, hospitals, and health centers. 7. IE&C PROGRAM The current preferred family size is nearly twice as high as needed to achieve a stable poplation. Also, a strong boy preference hinders a small family size as nearly all couples fuel they must have at least one or more sons. The IE&C program must, in the future, strive to emphasize the values of the small family and equality of the sexes. A second problem for the IE&C program to work. with in the: future is the large group of people who approves family planning, want no more children, but do not practice. The IE&C program must work to motivate these people to accept family planning And finally, for those who already practice, an IE&C program in the future must stress continuation of use. The IE&C campaign, to insure highest effectiveness, should be based on a detailed factor analysis of contraceptive discontinuance. In conclusion, Korea faces a serious unfavorable sociodemographic situation- in the future unless the population growth rate can be curtailed. And in the future, the decrease in fertility will depend solely on the family planning program, as the effect of other socio-economic factors has already been maximumally felt. A second serious factor to consider is the increasing number of eligible women due to the 1950's baby boom. Thus, to meet these challenges, the program target must be increased and the program must improve the effectiveness of its current activities and develop new programs.

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