• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.11
• /
• pp.1531-1535
• /
• 2002

It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex identification, because the procedures for cell preparation and FISH itself are complicated and time-consuming. The present study was undertaken to test a rapid FISH procedure for sexing chicken. A FISH probe was simultaneously synthesized and labeled with digoxigenin by polymerase chain reaction (PCR) targeting a 416 bp segment of the 717 bp XhoI family fragment which is repeated over 10 thousand times exclusively in the W chromosome. Sexing by FISH was performed on cytological preparations of early embryos, adult lymphocytes and feather pulps of newly hatched chicks. The DNA probe hybridized to all types of uncultured interphase as well as metaphase female but not male cells that had been examined. Moreover, consistent with the known site of the XhoI family, the hybridization signal was localized to the pericentromeric region of the W chromosome. We, therefore, conclude that the present PCR-based FISH can be used as a rapid and reliable sex identification procedure for chicken.

• Research in Community and Public Health Nursing
• /
• v.15 no.1
• /
• pp.44-55
• /
• 2004

Purposes: Purposes of this study were to assess the needs of health education of residents and to determine the difference according to their general characteristics in a community. Method: Subjects consisted of 599 residents who live in the vicinity of M Catholic University, S dong, M city, Jeollanam do. Data were collected by 6 research assistants, using a self reported questionnaire from July 1, 2003 to July 31, 2003. Results: Subjects were mostly female (58.8%). 20 30 years old(45.2%), married (70.1%), high school graduates (42.1%), have an occupation(73.1%). have incomes less than 1.000,000 won(54.2%) and have a religion(59.8%). and the family type of the subjects was primarily nuclear(64.1%). The need for education about 'family health care management and medical examination' was the highest in 8 domains. The need for education about 'cancer' was the highest and 'family health care', 'stress and strategies', 'introduction of medical examination' and 'oral care' followed in 41 items. The differences in the needs of health education in all domains was found according to subjects' general characteristics. Conclusion: Further research is needed to develop a health education program based on participants' needs and general characteristics.

• Proceeding of Spring/Autumn Annual Conference of KHA
• /
• 2004.11a
• /
• pp.111-116
• /
• 2004

This study is aimed to be used as basic data to plan Korean style cohousing for double-income families in the future by studying their lifestyle, housing needs and housing awareness on cohousing. The results of the study are as follows. 1) As for the life style of the subject families, it was a housewife that mainly does the household affairs despite of the status of double-income family. Also, as for the social activity, almost half of the respondents said that the spending in child-care facilities was not sufficient and wanted to send their children to ones within their neighborhood. 2) As far as housing awareness on cohousing concerned, among specific items, the child care area, the household affairs division area, and participatory awareness of residents in housing planning and management got higher scores, while as for awareness on utilizing common space, using the common hobby rooms, promenades and benches, exercise facilities, common yard, and study rooms got higher scores. When asked about the necessity for cohousing in Korea, they gave very positive answers, but the answer to their willingness to reside in it was less positive. 3) Summarizing the housing needs on cohousing, it turned out that more people wanted to reside in suburban area with others having common grounds with them as for a desirable residence environment. They wanted the neighborhood to be composed of 10${\sim}$29 families, and medium-density semi-detached house occupied by owners. They wanted a mixture of independent management and entrust management for managing common facilities, and common meal once a week. They wanted indoor exercise facilities to be more extended than other common facilities, and the house of $100m^{2}{\sim}130m^{2}$ with 3 bedrooms, 2 bath rooms, and a large living room and dining room.

• Journal of the Korean Home Economics Association
• /
• v.37 no.1
• /
• pp.45-65
• /
• 1999

The purpose of this study is to investigate the trend of the familism and the awareness of parent-supporting, showing how personal background variables affect the familism and how the familism affect the awareness of parent-supporting, The data were collected from May 20, 1998 till June 5, 1998 for 621 college students consisted of 300 male strdents and 321 female students. SOSS-PC was used for data analysis, and the data were tested by ANOVA, t-Test, Pearson's Correlation, Multiple Regression Analysis, and Duncan Test. The major findings are as follows: 1) The college students' familism generally tends to show low scores (M=2.79), indicating the modern familism-individualism. 2) The college students' awareness of parent-supporting is reported to be high(M=4.23); the highest awareness of parent-supporting is financial supporting, the next is service supporting, then emotinal supporting. 3) For personal background variables, male students rather than female students, the students who have parents with more monthly income, and those who have experience in living with grandparents tend to have more traditional familism. 4) Older students, the students with more traditional familism, those who find more satisfaction in family relations, and male students rather than female students tend to show higher awareness of parent-supporting. 5) In causal relations among the personal background variables, the familism, the awareness of parent-supporting, the variables with a significant impact on the familism are gender, parents' monthly income, and experience in living with grandparents, in order of impact. Age, the familism, satisfaction in family relations, and gender, in order of impact, affect the awareness of parent-supporting. Especially, the familism has the highest correlations with the awareness of financial, emotional, and service supporting, and shows considerable causal impact on the awareness of parent-supporting in general. That is to say, the more the familism, the more the awareness of the parent-supporting.

• The Korean Journal of Malacology
• /
• v.17 no.2
• /
• pp.105-115
• /
• 2001

These studies were conducted to compare the external radulae of seven species (Semisulcospira gottschei, S. coreana, S. forticosta, S. tegulata, S. ibertina, Koreanomelania nodifila and Koreoleptoxis globus ovalis) of freshwater snails in three genera of the family Pleuroceridae in Korea. Under a microscope of 100 magnifications, observation of seven species can be distinguished sharply between three genera of Semisuicospira, Koreanomelania and Koreoleptoxis, and the morphological characters of radulae can be used as taxonomic tool in genus level of Pleuroceridae. Besides SEM photograph of the radulae based on the morphological characters in seven species can be verified a new criteria for classification of genus' unit grade in accord with firsthand drawing sketch under a microscope of 100 magnifications. But it is difficult that all of the species can be detected sharply the difference of interspecies by comparisons of radulae in the family Pleuroceridae in Korea. Therefore, the morphological characters of radulae can not be set a criteria for classification of species level.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.11
• /
• pp.1933-1942
• /
• 2016

A novel ${\alpha}-glucoside$ hydrolase (named PspAG97A) from glycoside hydrolase family 97 (GH97) was cloned from the deep-sea bacterium Pseudoalteromonas sp. K8, which was screened from the sediment of Kongsfjorden. Sequence analysis showed that PspAG97A belonged to GH97, and shared 41% sequence identity with the characterized ${\alpha}-glucoside$ BtGH97a. PspAG97A possessed three key catalytically related glutamate residues. Mutation of the glutamate residues indicated that PspAG97A belonged to the inverting subfamily of GH97. PspAG97A showed significant reversibility against changes in salt concentration. It exhibited halophilic ability and improved thermostability in NaCl solution, with maximal activity at 1.0 M NaCl/KCl, and retained more than 80% activity at NaCl concentrations ranging from 0.8 to 2.0 M for over 50 h. Furthermore, PspAG97A hydrolyzed not only ${\alpha}-1,4-glucosidic$ linkage, but also ${\alpha}-1,6-$ and ${\alpha}-1,2-glucosidic$ linkages. Interestingly, PspAG97A possessed high catalytic efficiency for long-chain substrates with ${\alpha}-1,6-linkage$. These characteristics are clearly different from other known ${\alpha}-glucoside$ hydrolases in GH97, implying that PspAG97A is a unique ${\alpha}-glucoside$ hydrolase of GH97.

• Parasites, Hosts and Diseases
• /
• v.60 no.1
• /
• pp.1-6
• /
• 2022

The encystation of Acanthamoeba leads to the development of metabolically inactive and dormant cysts from vegetative trophozoites under unfavorable conditions. These cysts are highly resistant to anti-Acanthamoeba drugs and biocides. Therefore, the inhibition of encystation would be more effective in treating Acanthamoeba infection. In our previous study, a sirtuin family protein-Acanthamoeba silent-information regulator 2-like protein (AcSir2)-was identified, and its expression was discovered to be critical for Acanthamoeba castellanii proliferation and encystation. In this study, to develop Acanthamoeba sirtuin inhibitors, we examine the effects of sirtinol, a sirtuin inhibitor, on trophozoite growth and encystation. Sirtinol inhibited A. castellanii trophozoites proliferation (IC50=61.24 µM). The encystation rate of cells treated with sirtinol significantly decreased to 39.8% (200 µM sirtinol) after 24 hr of incubation compared to controls. In AcSir2-overexpressing cells, the transcriptional level of cyst-specific cysteine protease (CSCP), an Acanthamoeba cysteine protease involved in the encysting process, was 11.6- and 88.6-fold higher at 48 and 72 hr after induction of encystation compared to control. However, sirtinol suppresses CSCP transcription, resulting that the undegraded organelles and large molecules remained in sirtinol-treated cells during encystation. These results indicated that sirtinol sufficiently inhibited trophozoite proliferation and encystation, and can be used to treat Acanthamoeba infections.

• Korean Journal of Soil Science and Fertilizer
• /
• v.42 no.2
• /
• pp.88-97
• /
• 2009

This study was conducted to obtain the basic information to increase the practical use of soil survey data through the subdividing of lava shapes with soil sequences due to different elevations in Jeju. The numbers of soil series of lava topography had occupied many of whole soil series in Jeju. When its topography subdivide, it give more detailed soil information. The obtained results are as follows; The lava topography to subdivide lava topography were studied with 38 soil series according to elevation in Jeju. Division of elevation are less than 50m, 50m to 200m, and 200m to 400m and more than 400m. Name the depending on elevation, less than 50m is called lower part of lava, 50m to 200m is called middle part of lava, and 200m to 400m and more than 400m are called upper part of lava. The characteristics of lava subdivide are as follows; soil family texture of lower part of lava is fine silty to clayey, drainage classes are various, average of available soil depth is 75.3cm, average of gravely contents are 11.6%, average of slopeness is 7.2%, limiting factor are various and soil order are various. soil family texture of middle part of lava is fine silty to coarse silty, drainage classes are well to very well, average of available soil depth is 65.9cm, average of gravely contents are 14.7%, average of slopeness is 11.3%, limiting factor are ashy and soil order are Andisols and Inceptisols. Soil family texture of upper part of lave is fine silty, drainage classes are well, average of available soil depth is 72.8cm, average of gravely contents are 16.0%, average of slopeness is 14.9%, limiting factor are ashy and skeletal, and order are Andisols.

• Development and Reproduction
• /
• v.4 no.1
• /
• pp.87-93
• /
• 2000

Equine chorionic gonadotropin (eCG) consists of highly glycosylated noncovalently linked $\alpha$- and $\beta$-subunits and belongs to the glycoprotein hormone family that includes lutropin (LH), follitropin (FSH), and thyrotropin (FSH). eCG is a unique member of the gonadotropin family because it elicits response characteristics of both FSH and LH in other species than the hone. eCG is synthesized and secreted by trophoblastic cells of the endometial cups between 40 and 130 days of gestation. In the present study, mRNA expression ratio of eCG, eLH and eFSH $\alpha$- and $\beta$-subunints was investigated in the placenta and pituitary. mRNA was extracted from equine placenta on day 70 of gestation and from pituitary of male horse (27 month-old). When the expression of both subunit mRNAs of eCG in the equine placenta was compared by Northern blotting, the expression of the $\beta$ -subunit mRNA was relatively greater than that of the $\alpha$-subunit. And mRNA expression of $\alpha$-, LH $\beta$- and FSH $\beta$-subunits was analysed in the equine pituitary. An $\alpha$-subunit was revealed with a size of approximately 0.8 kb. FSH $\beta$-subunit mRNA also was detected out 1.8 kb. It is the same size of the FSH $\beta$ -subunit mRNA cloned. The intensity of $\alpha$-subunit mRNA was greater than that of the $\beta$-subunit suggesting that the expression of $\alpha$ -subunit was dominant in the equine anterior pituitary. Thus, the subunit mRNA levels seem to be independently regulated and their imbalance may account for differences in the quantities of $\alpha$- and $\beta$-subunits in the equine placenta and pituitary.

• Tuberculosis and Respiratory Diseases
• /
• v.67 no.6
• /
• pp.499-505
• /
• 2009

Background: Mycobacterial Interspersed Repetitive Units (MIRUs) that are located mainly in intergenic regions dispersed throughout the Mycobacterium tuberculosis genome. The selected MIRU loci, which were composed of a 12-locus set, demonstrated a high power for discrimination of Mycobacterium tuberculosis isolates collected from Kangwon province of Korea. To evaluate its ability to discriminate the M. tuberculosis strains, 45 clinical isolates were genotyped using the methods IS6110 RFLP and MIRU. Methods: All the samples were collected during the period from January 2007 to December 2007 from TB patients, who were residents and registered to a public health center of Kangwon Province in Korea. A total of 45 DNAs were extracted from clinical isolated mycobacterial strains and genotyped using IS6110 RFLP, the MIRU method. Results: We compared the 12-MIRU with IS6110 RFLP in the 45 samples, the 12-locus version offered less discriminatory power (Hunter-Gaston discriminatory index [HGDI]: 0.959 vs 0.998; 57.78% of clustered cases vs 8.89%). Conclusion: This 12-locus MIRU can be useful when additional combinations of other loci for genotyping M. tuberculosis in Korea where the Beijing family strains are dominant.