• Animal Bioscience
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• v.34 no.8
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• pp.1290-1302
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• 2021

Objective: Follicle selection is an important process in chicken egg laying. Among several small yellow (SY) follicles, the one exhibiting the highest expression of follicle stimulation hormone receptor (FSHR) will be selected to become a hierarchal follicle. The role of lncRNA, miRNA and other non-coding RNA in chicken follicle selection is unclear. Methods: In this study, the whole transcriptome sequencing of SY follicles with different expression levels of FSHR in Jining Bairi hens was performed, and the expression of 30 randomly selected mRNAs, lncRNAs and miRNAs was validated by quantitative real-time polymerase chain reaction. Preliminary studies and bioinformatics analysis were performed on the selected mRNA, lncRNA, miRNA and their target genes. The effect of identified gene was examined in the granulosa cells of chicken follicles. Results: Integrated transcriptomic analysis on chicken SY follicles differing in FSHR expression revealed 467 differentially expressed mRNA genes, 134 differentially expressed lncRNA genes and 34 differentially expressed miRNA genes, and sosondowah ankyrin repeat domain family member A (SOWAHA) was the common target gene of three miRNAs and one lncRNA. SOWAHA was mainly expressed in small white (SW) and SY follicles and was affected by follicle stimulation hormone (FSH) treatment in the granulosa cells. Knockdown of SOWAHA inhibited the expression of Wnt family member 4 (Wnt4) and steroidogenic acute regulatory protein (StAR) in the granulosa cells of prehierarchal follicles, while stimulated Wnt4 in hierarchal follicles. Overexpression of SOWAHA increased the expression of Wnt4 in the granulosa cells of prehierarchal follicles, decreased that of StAR and cytochrome P450 family 11 subfamily A member 1 in the granulosa cells of hierarchal follicles and inhibited the proliferation of granulosa cells. Conclusion: Integrated analysis of chicken SY follicle transcriptomes identified SOWAHA as a network gene that is affected by FSH in granulosa cells of ovarian follicles. SOWAHA affected the expression of genes involved in chicken follicle selection and inhibited the proliferation of granulosa cells, suggesting an inhibitory role in chicken follicle selection.

• ALGAE
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• v.20 no.4
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• pp.271-278
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• 2005

Evidence is presented to demonstrate that the type of Delesseria kurilensis Ruprecht belongs to Congregatocarpus rather than Tokidadendron (both genera of the red algal family Delesseriaceae), and the new combination of Congregatocarpus kurilensis (Ruprecht) M.J. Wynne is proposed.

• Journal of the Korean Institute of Intelligent Systems
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• v.9 no.5
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• pp.550-554
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• 1999

We will define a fuzzy quasi-proximity space and give some examples of it. We show that the family M(X, C) of all fuzzy quasi-proximities on X which induce C is nonempty. Moreover we will study the relationship between the category of fuzzy closure spaces and that of fuzzy quasi-proximity spaces.

• The Pure and Applied Mathematics
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• v.15 no.3
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• pp.221-227
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• 2008

We introduce and study the new concepts of interior and closure operators on strong generalized neighborhood spaces. Also we introduce and investigate the concept of sgn-continuity on SGNS.

• Journal of the Korean Society of Costume
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• v.61 no.10
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• pp.72-88
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• 2011

This study is about the costume relics that were excavated from M10 of the 6th site, M13 and M14 of the 8th site among all the Runghai(龍海) ancient tomb sites that were known as the royal family's tombs of Balhae, which were built from the late 8th century to the early 9th century. These costume relics were also introduced in the academic journal of Chinese archaeology(考古) 6 in 2009. The summary of the results that focused on finding features of the costume relics and its meaning were as follows: 1. Male and female statues, both of which were excavated from the Runghai ancient tomb sites, had the shape and composition of the government official's costumes such as the Danryeong(團領), Bokdu, and Gwadae. The female's hairstyle and accessories were quite similar to other costume relics of Balhae. In particular, the male statues wearing the Danryeong and Bokdu were considered as the normal figures of government officials of Balhaeafter the mid 8th century. 2. The female statue wearing the male attire is considered as a maid, and we can confirm that women dressing up like a man was a popular trend in Tang(唐), and this trend was introduced to Balhae. 3. The back flap(垂脚) of Bokdu that the male statue is wearing in M10 of the 6th site, has a shape that has not been found in the ancient relics of both Balhae and Tang. Therefore, it is considered as a unique shape of Bokdu of Balhae. However, it needs to be observed more and discussed in the future. 4. In regards to the Gwadae, the outside of the Gwadae is decorated with jade and has an embossed carving that is gold inside. The Gwadae of the Runghaisites has a unique design and it is distinguished from other ancient relics. 5. The gold trefoil crown and the leather conical hat that were found in M14 of the 8th site can be seen as the basic composition of official's hats in ancient Korea. Also, the motif of the gold trefoil is closely related to Anthemion that is often seen in the relics of the Three Kingdom period. Thus, we can assume according to this important finding that the style of ancient Korea official's hats came from either the king or a royal family of Balhae after the mid 8th century.

• Korean Journal of Community Nutrition
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• v.15 no.6
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• pp.717-726
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• 2010

This study was conducted to investigate health status and nutrient intakes among 5th grade elementary students at Korea Centers for Disease Control and Prevention 2010. This study was cross-sectional study on 1,384 children (687 boys, 697 girls) from nine elementary schools located in Seoul and Gyeonggi province. The average height, weight and BMI were 145 cm, 40.6 kg $19.2\;kg/m^2$ for boys and 145.4 cm, 38.2 kg, $18.0\;kg/m^2$ for girls. The prevalence of overweight and obesity were 11.3%, 5.2%, respectively. Serum AST, ALT, glucose, HDL-Cholesterol and RBC levels were significantly higher, while TG levels was significantly lower for boys than for girls. The average energy intake was 1772.4 kcal, which was 98.7% of Estimated Energy Requirement (EER). The boys consumed more energy intake (1800.4 kcal) than the girls (1744.7 kcal). Also, they took insufficient calcium and folate (69.1% and 83.3% of Recommended Intake (RI)) and excess sodium (297.6% of Adequate Intake (AI)) and 85.1% of the subjects had breakfast everyday. In general, the proportion of the children who consumed fruits and vegetables at least once or more a day was low. There was a higher proportion of children in boys who had ramyun and milk with sufficient physical activity than those in girls. As a result of this study, we can find risk factors on obesity and metabolic disorders, and the results can be used for an evidence of nutrition education program and the intervention program.

• Journal of Life Science
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• v.17 no.1 s.81
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• pp.6-11
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• 2007

[ $\beta$ ]-Fructofuranosidases, a family 32 of glycoside hydrolases (GH32), share three conserved domains including the W(L/M)(C/N)DP(Q/N), FRDPK, and ECP(D/G) motifs. The functional role of the conserved acidic residues within three domains of levan fructotransferase, one of the $\beta-fructofuranosidases$, from Microbacterium sp. AL-210 was studied by site-directed mutagenesis. Each mutant was overexpressed in E. coli BL21(DE3) and purified by using Hi-Trap chelating affinity chromatography and fast performance liquid chromatography. Substitution of Asp-63 by Ala, Asp-195 by Asn, and Glu-245 by Ala and Asp decreased the enzyme activity by approximately 100-fold compared to the wild-type enzyme. This result indicates that three acidic residues Asp-63, Asp-195, and Glu-245 play a major role in catalysis. Since the three acidic residues are present in a conserved position in inulinase, levanase, levanfructotransferase, and invertase, they are likely to have a common functional role as nucleophile, transition state stabilizer, and general acid in $\beta-fructofuranosidases$.

• Korean Institute of Interior Design Journal
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• v.23 no.3
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• pp.21-28
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• 2014

The purpose of this study is to analysis the change of rural house type in Gyeongbuk province. There are 13 Gun(a kind of administrative district) areas in Gyeongbuk province. According to definition of rural area, the scopes of the research of rural houses limited the 12 places rural area(Gun area, excluded Ulleung-Gun) of Gyeongbuk province. The method of study is to compare and analyze about housing situation, structure of house, housing type and housing area etc. through the statistical data of each Gun area and other various data etc. during these 5 years. As a results of the analysis : 1) The supply ratio of housing is steadily decreasing in rural areas. 2) The houses of rural areas are changing from a detached house to multi-household house and small apartment by development near the rural area. 3) The number and area of commercial buildings are gradually increasing because of urbanization of rural areas. 4) The most houses inside area scopes in the rural area was from $60m^2$ to $85m^2$ area. And the family types of rural house are changing from large family to nuclear family and single households. 5) The structure of rural houses is changing from the brick house type to lightweight steel construction house because of cost-cutting of construction and easy way to construct etc.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.4
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• pp.571-582
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• 1994

Three livestock/crop demonstration-cum-training farms have been established on plots of half, one and two acres, typical of the "Kandyan Forest Garden System" Vegetables, bananas, pepper, coffee, coconut and fruit trees are widely spaced, for intercropping with grass, and have been surrounded with live fences that also provide fodder for livestock to increase the family income. Each unit is operated by a selected employee and his family under a monthly incentive scheme based upon the gross margin. On these farms the technical parameters in dairying are better than elsewhere in the Mid-Country. Economic performance over 1985-1992 showed that dairying contributed most to the total gross margin of the half, one and two acre units, i.e. 31, 63 and 69%, respectively. Next came crops (29%, 37% and 19%), poultry (22%, 0% and 9%), and goats (18%, 0% and 3%). In the three farms the cash income per Sri Lankan Rupee spent was 1.5, 4.6 and 2.1, respectively. The overall ratio was 3.2 for dairying, 1.1 for poultry, 4.5 for goats and 9.9 for crops. Actual family labour in the three farms was 548, 548 and 639 days, compared to the 270, 330 and 440 days anticipated in the initial feasibility study. The average incentive payments, which were 20% (half acre), 61% (one acre) and 133% (two acres) of the parastatal salary of the employee, were only insufficient for the extra labour applied in the half acre unit. Dairying and goats proved to be attractive cash earners with a domestic fuel were important benefits. Poultry did little to improve farm income.

• Journal of Nutrition and Health
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• v.36 no.7
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• pp.769-776
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• 2003

This study has analyzed the association of physical activity and television watching time with obesity in 30-50 aged women from middle income town in Busan city area. Data were obtained using a questionnaire, including information about physical activity, TV watching time and health habit; social data regarding educational level and monthly income; family history of chronic condition. Height and weight were measured. Outcome variable was obesity, defined as a body mass index 25 kg/m$^2$ or greater. Odds ratios (OR) for obesity were estimated by multivariate logistic regression, and interpreted as a relative risk of obesity. The prevalence of obesity was 11.8%. The mean BMI of obese women was 26.9 kg/m$^2$. The OR of obesity was higher in subject of 40s than 30s. Subjects who had higher educational level presented lower OR than those of elementary or middle school education. Family income was not associated with obesity. Women having a family history of chronic condition had higher risk of obesity than those who did not have it. The OR was lower in active people at work (OR = 0.51; 95% CI = 0.26 - 0.98) than in inactive ones, and the ORs associated with regular exercise or activity at leisure time were not statistically different. However, subjects watching TV $\geq$ 3.5 hr/day showed higher OR (OR = 2.34; 95% CI = 1.16 - 4.74), compared with those watching TV $\geq$ 1.5 hr/day. Association of the joint classification of physical activity variables with obesity was estimated. The highest relative risk of 5.99 was in women in physically inactive at work and high category of TV watching ($\geq$ 3.5 hr/day). Even in women in active at work, the watching TV $\geq$ 3.5 hr/day made them have the high OR (OR = 2.44; 95% CI = 1.03 - 5.77). And at each time level of TV watching, the increasing activity level at work was associated with lower OR for obesity. These findings suggest that both TV watching time and physical activity at work were related to obesity in adult female, each with independent effects on obesity. TV watching time seems to be a significant factor that could partly evaluate the energy expenditure.