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Antioxidant Activity and Chemical Characteristics of Orostachys malacophyllus and Fermented Orostachys malacophyllus (와송과 발효 와송 추출물의 이화학적 특징 및 항산화 활성)

  • Ahn, Hee-Young;Choe, Da-Jeong;Cho, Young-Su
    • Journal of Life Science
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    • v.25 no.5
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    • pp.577-584
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    • 2015
  • Orostachys malacophyllus grow on the old roofing tile or on the rock of mountain and is belong to Crassulaceae family. After air drying for Orostachys malacophyllus (OM), using the mixture of lactic acid bacteria (Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus) was fermented (FOM). OM and FOM extracted using water (W), ethanol (E) and methanol (M) and were measured extracts yield, pH and Brix. Extracted OM and FOM were tested by in vitro experimental models of α,α´-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity, Fe/Cu reducing power, linoleic acid peroxidation using ferric thiocyanate and thiobarbituric acid (TBA) methods and peroxidation of rat liver homogenate. In addition, the bioactive materials (phenolic compounds, flavonoids and minerals) were measured. The highest phenolic compounds and flavonoids were OME 122.2 mg/100 g and OME 84.0 mg/100 g. OM and FOM′s major minerals were K, Ca and Mg. The highest free radical scavenging activity showed in FOMM (93.9%), OMM (93.4%), FOME (92.1%) and OME (91.9%) at 0.5% additional level. Fe reducing powers were strong in FOME and FOMM and Cu reducing powers were strong in OME and FOMM. Antioxidant activities on lipid peroxidation using rat homogenate as measured by TBARS method showed strong in FOME and on lipid peroxidation of linoleic acid as measured by ferric TBA method showed strong in OME and FOME and measured by ferric thiocyanate showed strong in FOME and FOMM.

Ultrastructure of the Fertilized Egg Envelope from Hyphessobrycon serpae, Characidae, Teleost (경골어류 카라신과 Hyphessobrycon serpae의 수정란 난막 미세구조)

  • Kim, Dong-Heui;Deung, Young-Kun;Lee, Kyu-Jae
    • Applied Microscopy
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    • v.35 no.2
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    • pp.89-96
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    • 2005
  • The ultrastructures of the fertilized egg envelope from Hyphessobrycon serpae belonging to Characidae was studied using scanning and transmission electron microscopes to get systematic fundamental data for classification of species and to confirm whether micropyle is a common trait of Characidae or not. The fertilized egg was of colorless, transparent, spherical, adhesive and demersal type. There were not oil droplets in vitelline membrane and attached structures in the outside of fertilize egg envelope. The egg envelope had a single micropyle resembling the pathway of sperm in the area of the animal pole. The micropyle was surrounded by 13 to 15 protruded lines of the egg envelope in a radiated form. The outer surface of fertilized egg envelope was covered by reticular adhesive fibrous structures and irregularly arranged by pore canals. The fertilized egg envelope consisted of three distinct layers an outer adhesive fibrous layer with high electron density, a middle layer with pore canals, and an inner layer consisting of 6 to 7 lamellae alternating layers with interlamellae of lower electron density. These ultrastructural characters of fertilized egg envelope form Hyphessobrycon serpae can be utilized in taxonomy of teleost, and as fundamental data for study on early development of fertilized egg. It seems that the morphology of micropyle is a common trait of Characidae

Work Hours and Cognitive Function: The Multi-Ethnic Study of Atherosclerosis

  • Charles, Luenda E.;Fekedulegn, Desta;Burchfiel, Cecil M.;Fujishiro, Kaori;Hazzouri, Adina Zeki Al;Fitzpatrick, Annette L.;Rapp, Stephen R.
    • Safety and Health at Work
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    • v.11 no.2
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    • pp.178-186
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    • 2020
  • Background: Cognitive impairment is a public health burden. Our objective was to investigate associations between work hours and cognitive function. Methods: Multi-Ethnic Study of Atherosclerosis (MESA) participants (n = 2,497; 50.7% men; age range 44-84 years) reported hours per week worked in all jobs in Exams 1 (2000-2002), 2 (2002-2004), 3 (2004-2005), and 5 (2010-2011). Cognitive function was assessed (Exam 5) using the Cognitive Abilities Screening Instrument (version 2), a measure of global cognitive functioning; the Digit Symbol Coding, a measure of processing speed; and the Digit Span test, a measure of attention and working memory. We used a prospective approach and linear regression to assess associations for every 10 hours of work. Results: Among all participants, associations of hours worked with cognitive function of any type were not statistically significant. In occupation-stratified analyses (interaction p = 0.051), longer work hours were associated with poorer global cognitive function among Sales/Office and blue-collar workers, after adjustment for age, sex, physical activity, body mass index, race/ethnicity, educational level, annual income, history of heart attack, diabetes, apolipoprotein E-epsilon 4 allele (ApoE4) status, birth-place, number of years in the United States, language spoken at MESA Exam 1, and work hours at Exam 5 (β = -0.55, 95% CI = -0.99, -0.09) and (β = -0.80, -1.51, -0.09), respectively. In occupation-stratified analyses (interaction p = 0.040), we also observed an inverse association with processing speed among blue-collar workers (adjusted β = -0.80, -1.52, -0.07). Sex, race/ethnicity, and ApoE4 did not significantly modify associations between work hours and cognitive function. Conclusion: Weak inverse associations were observed between work hours and cognitive function among Sales/Office and blue-collar workers.

Health and Nutritional Factors Related to Hypertension of Subjects Aged Over 50 in High Income Class - Based on the 2005 Korea National Health and Nutrition Examination Survey - (50세 이상 고소득층 성인 및 노인의 고혈압 관련 건강 및 영양 요인에 관한 연구 - 2005년 국민건강영양조사 결과에 근거하여 -)

  • Ahn, So-Hyun;Son, Sook-Mee;Park, Jin-Kyung
    • Journal of the Korean Dietetic Association
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    • v.15 no.3
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    • pp.311-327
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    • 2009
  • This study was performed to determine the health and nutritional risk factors associated with hypertension in Koreans over the age of 50 in a high-income class (more than twice as much family income as the 2005 Korean minimum cost of living, 668,540 Won). A total of 505 subjects aged over 50 from the 2005 Korean National Health and Nutrition Examination Survey (KNHANES) were divided into two groups: A hypertension group (HG) (N=151, Systolic Blood Pressure ${\geq}$140 mmHg or Diastolic Blood Pressure ${\geq}$90 mmHg) and normal group (NG) (N=354). Subjects who took hypertension medicines or underwent diet therapy were excluded. In HG, mean daily alcohol intake and the amount of alcohol consumption per one occasion were significantly higher than in NG, respectively. A greater number of hypertension subjects answered that they drank alcohol to reduce stress as compared to normal subjects. HG also took fewer dietary supplements than NG. Mean body mass index (BMI), waist circumference, fasting blood sugar level, and 2 hour postprandial blood sugar following a glucose load were significantly higher in HG than in NG, respectively. Also, iron, thiamin, and niacin intakes and the consumption frequency of seaweeds were significantly lower in HG than in NG, respectively. Finally, obesity (BMI ${\geq}$25 $kg/m^2$), abdominal obesity (waist circumference ${\geq}$90 cm for males, ${\geq}$ 80 cm for females), high blood sugar level 2 hours after an oral glucose load (${\geq}$140~200 mg/dl), and hypertriglyceridemia (serum TG ${\geq}$200 mg/dl) were related to a significantly higher risk of hypertension in the subjects (odds ratio: 1.884~3.040). In conclusion, dietary factors such as higher alcohol consumption; lower intakes of iron, thiamin, and niacin; lower consumption frequency of seaweeds; and metabolic syndrome were associated with hypertension in the study subjects.

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Improvement Characteristics of Bio-active Materials Coated Fabric on Rat Muscular Mitochondria

  • Lee, Donghee;Kim, Young-Won;Kim, Jung-Ha;Yang, Misuk;Bae, Hyemi;Lim, Inja;Bang, Hyoweon;Go, Kyung-Chan;Yang, Gwang-Wung;Rho, Yong-Hwan;Park, Hyo-Suk;Park, Eun-Ho;Ko, Jae-Hong
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.3
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    • pp.283-289
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    • 2015
  • This study surveys the improvement characteristics in old-aged muscular mitochondria by bio-active materials coated fabric (BMCF). To observe the effects, the fabric (10 and 30%) was worn to old-aged rat then the oxygen consumption efficiency and copy numbers of mitochondria, and mRNA expression of apoptosis- and mitophagy-related genes were verified. By wearing the BMCF, the oxidative respiration significantly increased when using the 30% materials coated fabric. The mitochondrial DNA copy number significantly decreased and subsequently recovered in a dose-dependent manner. The respiratory control ratio to mitochondrial DNA copy number showed a dose-dependent increment. As times passed, Bax, caspase 9, PGC-$1{\alpha}$ and ${\beta}$-actin increased, and Bcl-2 decreased in a dose-dependent manner. However, the BMCF can be seen to have had no effect on Fas receptor. PINK1 expression did not change considerably and was inclined to decrease in control group, but the expression was down-regulated then subsequently increased with the use of the BMCF in a dose-dependent manner. Caspase 3 increased and subsequently decreased in a dose-dependent manner. These results suggest that the BMCF invigorates mitophagy and improves mitochondrial oxidative respiration in skeletal muscle, and in early stage of apoptosis induced by the BMCF is not related to extrinsic death-receptor mediated but mitochondria-mediated signaling pathway.

Characteristics of Breast Cancer Patients, their Understandings and Attitude towards the Disease (유암환자의 제 특징 및 유암에 관한 태도와 그 인식도)

  • 노유자
    • Journal of Korean Academy of Nursing
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    • v.7 no.1
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    • pp.30-38
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    • 1977
  • Breast cancer is one of the most feared health problems in women ; Recent studies revealed that it had come up to be the second most in this country and high prevalent disease in the western countries among breast disease in women. However, early detection of the cancer mass is known to be easier than in many other malignancies. This study was performed to investigate the various characteristics of patients of breast cancer ; by the structural variables, menstrual, marital, and child bearing, and also their understandings and attitude towards the disease. A hundred any in- and out- patients of 51. Mary's Hospital and National Atomic Institute, Seoul were sampled. Data were gathered through direct interview by the researcher from February 1976 to January 1977 and the clinical records were used as references. Results are as follows ; 1. Breast cancer revealed to be most prevalent in the forties ; average age of 45.2 years. 2. The average age of menarche revealed to be 15.4 years ; the largest group were the 16-20 years (N=75, 68.2%). In 55 cases (50%) menstruation were normal, 38 (34.5%) postmenopausal and only in 17 (15.5%), menstruation revealed irregularity. 3. The average marital age revealed to be 22.3 years ; the largest group were 21-25 group (N=43, 39.1%). The average duration of marital life revealed to be 24.7 years ; 11-20 years group were the largest (M=34, 30.9%). 4. Most of the patients revealed to have pregnancy experiences(N=100, 90.9%) ; the average rate of experience were 5, 3 times the largest group were 3.4 times group(N= 32, 29.1%). 54 patients (49.1%) revealed to have had abortion experience ; the average were 3.4 times. 5. The largest group(N=77, 70%) had been breast feeding : followed by mixed feeding (N=12, 10.9%) and artificial feeding(N=10, 9.1%). 6. Personal health history revealed that in 20 patients (18.2%) revealed to have the past history of Purulent mastitis, 5 patients(4.5%) of breast cancer and 3 patients(2.7%) of uttering cancer family history. 7. In the one half (N=56, 50.9%, they had some information about breast cancer :27 (24.5%) by mass media, 12 (10.9%) through personal contacts and 17 (15.5%) were not able to classify the source of information. 8. In 55 cases (50%) the canoe, mass were discovered incidentally, in 39 cases (35.5%) by manual detection by self, in 10 cases (9%) by others and in 6 cases(5.5%) by observing subjective symptoms. 9. The average duration lapsed between the discovery of cancer mass and the visit to the hospital revealed to be 9.4 month. Chief reason for the delay revealed to be the non-chaplaincy due to the absence of pain(N=50, 45.5 %) followed by the administration of herb and commercial medication (N=19, 17.3%). 10. The left side breast was more affected than the right side breast, represent by 60 cases in the left and 39 cases in the right. The most frequent site of the breast cancer was the upper- outer quadrant in 53 cases (47.7%), and followed by the center in 20 cases(18 %), and the upper inner quadrant, in 19 cases (17.1%). There was / cases of bilateral carcinoma. The most prominent symptom was painless mass.

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A Study on the Occurrences and Causes of Accidents in Lower Grade Elementary School Children (초등학교 저학년 아동들의 안전사고 발생 실태 및 관련요인 분석)

  • 김소선;이은숙
    • Journal of Korean Academy of Nursing
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    • v.29 no.1
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    • pp.117-126
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    • 1999
  • Accidents involving children are an important cause of death and disability. They also have enomorous financial implications. In order to prevent childhood accidents, research and education for safety should be strengthened. The purpose of this study was to determine how often young children have accidents and what factors affect the accident rate. The sample consisted of 771 children who were in the second, third and forth grades of two elementary schools located in Kyung-gi Province. One school had students from middle class families living in apartment complexes and the other, students from lower income families mainly living in single houses. The questionnaires included items on the occurance of accidents and the parents' attitudes regarding accidents during the academic year from March 1997 to February 1998. The Questionnaires were distributed to conventiently selected students to be compeleted by their parents and collected during the period of May 28, to June 6, 1998. The data were analyzed using SAS PC statistical package. The results of the study are as follows ; 1. Of 771 student subjects, 393 had 887 accidental injuries during the study period. 2. The month, the day and the time with the highest accident rate were May, Sunday, and between 1 and 4 p. m. each. 3. In the analysis of the location where the injury took place, the most frequent place was on around their homes followed by school and, then, inside the home 4. Most of the accidents were caused by carelessness on the part of the children and the most frequent type of injury was an abrasion. 5. Children most injured their legs 6. They were treated at home most often and usually emergency treatment was performed by family members with, disinfection being the main type of first aid. Cost of the treatment ranged from 8,000 to 20,000 won in most cases. 7. House type and parents' education level were statistically significant in chi-square analysis. 8. Parents educate their children about traffic safety most frequently followed by fire safety and, then, prevention of violence. 9. Parents think that prevention of violence should be the most important part of injury prevention education both at school and home. 10. To identify factors related to accident occurrence, multiple logistic regression was performed and the main factors were birth order and house type.

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The Comparative Assessment of Cold Tolerance of Broad-leaved Evergreen Trees by Low Temperature Treatment (저온처리에 따른 국내 상록활엽수종의 내한성 비교 평가)

  • Jin, Eon-Ju;Yoon, Jun Hyuck;Bae, Eun-Ji;Choi, Myung Suk
    • Journal of Korean Society of Forest Science
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    • v.108 no.4
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    • pp.484-492
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    • 2019
  • The aim of the present study was to compare the cold tolerance of seven different types of trees growing in southern Korea to select evergreen broad-leaved trees that can be used as street trees in large land areas experiencing climate change. The trees compared were the thorn tree, Cinnamomum camphora, Camellia japonica, Machilus thunbergii, Dendropanax morbifera, Daphniphyllum macropodum Miq., Quercus glauca Thunb., and Raphiolepis indica. When the trees were subjected to low temperature treatment, their electrolyte elution volume values appeared to increase with the decreases in the treatment temperature. The analysis of the cold tolerance of each type of tree was based on the estimated temperatures in the following order: C. japonica (-11.586℃) > R. indica (-9.348℃) > Q. glauca (-8.719℃) > M. thunbergii (-8.090℃) > D. macropodum (-7.409℃) > D. morbifera (-7.085℃) > C. camphora (-6.995℃). The relative cold tolerance difference found in the seven tree species was more than 5℃, as evaluated previously. In the Lauraceae family, the difference in cold tolerance was more than 2℃, even in the same species. The analysis showed that trees with excellent cold tolerance included Q. glauca Thunb., C. japonica, R. indica, and the thorn tree. This knowledge is required for the evaluation of the possibility of the survival of trees under cold temperature conditions in cities.

A Sexual Knowledge and Attitude on the Exposure to PC Pornography of the Middle School Boys in Busan (남자중학생의 컴퓨터음란물 접촉자와 비접촉자간의 성지식과 태도의 차이)

  • Kim Y. H.;Lee H. Z.;Jung H. M.
    • Child Health Nursing Research
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    • v.7 no.1
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    • pp.62-73
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    • 2001
  • The purpose of the study is to identify the differences about knowledge and attitude between the subjects exposed to pc pornography and the control group not exposed to pc pornography. The study was executed from July 12 through July 16. The subjects were 423 middle school boys. The comparison points were their ethical sexual knowledges and sexual attitudes. The collected data was analysed by SPSS WIN(including multiplicand, percentage, χ²-test, revised Fisher and t-test). The results of their study were as follows ; 1. The pc-related characteristics of the subjects. The subjects exposed pc pornography showed 51.8% rate of exposure to printed pornography. The control group showed the rate of 26.4%. The former group owned their pc(81.5%), however, the latter group showed 66.7%. The former group set their pc sets in their study rooms(66.1%) however, the latter group installed those in their study rooms(73.6%). The former group experienced internet pc communications(62.5%),however, the latter group showed 40.2%. The former group made use of their pc sets in recreation(entertainment) programs(77.0%), however, the latter group showed the rate of 67.8%. The former group showed that their school performance levelled up after their pc manipulation(80.0%), however, the latter group showed the rate of 86.2%. The former group replied that their pc manipulation contributed to their friendship(50.3%), however, the latter group showed the higher rate of 74.7%. The both groups replied that their family dialogs and contacts rather reduced (78.9% and 78.2% respectively). The both groups revealed that they reduced the TV watching the rate 76.5% and 48.3% respectively. The differences between two groups were surveyed in the level of 95% significance and the items such as 'contacts to printed pornography, pc possession or none, pc use rate, friendship occasions and the reduced time of TV watching' showed the significant differences. 2. The differences of two groups' sexual knowledges. As to the sexual knowledges, the both groups showed 41.1 and 34.1 points against 100 points respectively. The statistical differences were significant(t=2.72, p=.007). The 5 items among 17 showed the significant differences between two groups. 'The concept of masturbation' was χ²=5.033, p=.025. 'The prejudice to masturbation' showed χ²=9.902, p=.002 'The difference between female and male as to sexual excitement' showed χ²=7.985, p=.005. 'The positiveness of masturbation' showed χ²=10.205, p=.001. 'The differences between two sexes as to sexual impulse and sexual desire' showed χ²=8.463, p=.004. In conclusion, The former group showed the higher knowledges than the latter group. 3. The differences of two groups' sexual attitudes. The 4 items such as 'the curiosity to the other sex'(t=2.22, p=.027), 'the attention to pornography'(t=3.39, p=.001), 'the permission to pre-marriage intercourse'(t=2.15, p=.032) and 'the preference to the female body exposure'(t=2.67, p=.008) showed the differences between two groups as to sexual attitudes.

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Effect of Gentro $F^{(R)}$ on Abdomen Fat (젠트로 $F^{(R)}$의 복부지방에 미치는 영향)

  • Lee, Gye-Won;Lee, Joo-Yeon;Yu, Byong-Yeon;Byun, Mu-Won
    • YAKHAK HOEJI
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    • v.51 no.6
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    • pp.455-462
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    • 2007
  • The participants were recruited 77 healthy adult persons aged between 20 and 50 who have BMI above $25kg/m^2$ in this study. All subjects were randomly assigned to the Gentro $F^{(R)}$ drinking group and non-drinking group. We were investigated about abdomen fat decreasing effect of Gentro $F^{(R)}$, distillate of pepper. Total fat area (TFA), subcutaeneous fat area (SFA) and visceral fat area (VFA) and subcutaneous fat area/visceral fat area ratio (SVR) has been assessed by obesity index (BMI, body fat percent, waist circumference), CT scan taken on the $L4{\sim}5$ position, umbilicus level and blood analysis evaluated during 3 month. The obesity indexes were a little decreased in two groups. However, the waist circumference (WC) was decreased about 5% in drinking group after 3 month and there was significant difference in the change 2 and 3 between two group. TFA and VFA were significantly decreased in the drinking group compare to the non-drinking group (p<0.05) and in umbilicus and $L4{\sim}5$ position, were $370.33{\pm}92.30,\;380.35{\pm}97.64\;and\;114.90{\pm}44.91,\;101.99{\pm}41.24$, respectively. These result means that Gentro $F^{(R)}$ is effective on abdomen fat decreasing. Total cholesterol were decreased without difference in both groups. Lipid factors (TG, HDL- and LDL-cholesterol) were decreased without significance. The mea surement of SVR taken on the $L4{\sim}5$ position were more significantly correlated with obesity index that BMI was 0.787, body fat percentage was 0.754 than on the umbilicus level. The TFA and VSA was correlated with ALP, ASP, Albumin, Insulin and the SVR was correlated with the indexes of liver function and lipid factor found in the blood. Therefore, it is conclude that Gentro $F^{(R)}$ drinking leads to a decrease in abdominal obesity by reducing waist circumference and visceral fat area.