• Title/Summary/Keyword: m-family

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Family Life Style Changes According to the Householder's Job Status (가장의 실직에 따른 가정생활 변화)

  • 김용숙;서혜경;이영숙
    • Korean Journal of Human Ecology
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    • v.1 no.2
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    • pp.12-22
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    • 1998
  • The purpose of this study was to examine the effects of householder's job status m the family life style. Data was collected from 475 parents in Chonbuk province by using a self administered questionnaire from June 10 to 30, 1998. Frequencies, percentages, and averages were calculated T-test, Pearson's correlation coefficients, and stepwise multiple regression analysis were followed. The results were as follows; 1. The families who suffered householders' job loss were 1/4 of the respondents and over 4/5 of the families felt economic stresses. 1/3 of the respondents had debts and 1/5 had not any savings. Most of the families were in good or normal marriage relationships, experienced economic crises, and had sparing habit. 2. In out-of-job families, couple cohension, couple satisfaction, and total marriage relationships were lower than in-job families. Also, out-of-job families spared more in buying and using clothes and foods, total clothes living and total food living. 3. In out-of-job families, the economic pressures on marriage relationships, clothes living, and food living were stronger than in-job families. 4. Family resources such as savings, ordinary marriage relationships, and sparing habit effected on the marriage relationships, clothes living, and food living in out-of-job families and in-job families. (Korean J Human Ecology 1(2):12∼22. 1998)

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Purification and Characterization of Glycerate Kinase From the Thermoacidophilic Archaeon Thermoplasma acidophilum: An Enzyme Belonging to the Second Glycerate Kinase Family

  • Noh, Mi-Young;Jung, Jin-Hwa;Lee, Sun-Bok
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.4
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    • pp.344-350
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    • 2006
  • Thermoplasma acidophilum is a thermoacidophilic archaeon that grows optimally at $59^{\circ}C$ and pH 2. Along with another thermoacidophilic archaeon, Sulfolobus solfataricus, it is known to metabolize glucose by the non-phosphorylated Entner-Doudoroff (nED) pathway. In the course of these studies, the specific activities of glyceraldehyde dehydrogenase and glycerate kinase, two enzymes that are involved in the downstream part of the nED pathway, were found to be much higher in T. acidophilum than in S. solfataricus. To characterize glycerate kinase, the enzyme was purified to homogeneity from T. acidophilum cell extracts. The N-terminal sequence of the purified enzyme was in exact agreement with that of Ta0453m in the genome database, with the removal of the initiator methionine. Furthermore, the enzyme was a monomer with a molecular weight of 49kDa and followed Michaelis-Menten kinetics with $K_m$ values of 0.56 and 0.32mM for DL-glycerate and ATP, respectively. The enzyme also exhibited excellent thermal stability at $70^{\circ}C$. Of the seven sugars and four phosphate donors tested, only DL-glycerate and ATP were utilized by glycerate kinase as substrates. In addition, a coupled enzyme assay indicated that 2-phosphoglycerate was produced as a product. When divalent metal ions, such as $Mn^{2+},\;CO^{2+},\;Ni^{2+},\;Zn^{2+},\;Ca^{2+},\;and\;Sr^{2+}$, were substituted for $Mg^{2+}$ the enzyme activities were less than 10% of that obtained in the presence of $Mg^{2+}$. The amino acid sequence of T. acidophilum glycerate kinase showed no similarity with E. coli glycerate kinases, which belong to the first glycerate kinase family. This is the first report on the biochemical characterization of an enzyme which belongs to a member of the second glycerate kinase family.

Ultrastrucure of Germ Cells during Spermatogenesis and Some Characteristics of Sperm Morphology in Male Mytilus coruscus (Bivalvia: Mytilidae) on the West Coast of Korea

  • Kim, Jin-Hee;Chung, Ee-Yung;Choi, Ki-Ho;Park, Kwan-Ha;Park, Sung-Woo
    • The Korean Journal of Malacology
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    • v.26 no.1
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    • pp.33-43
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    • 2010
  • The ultrastructure of germ cells during spermatogenesis and some characteristics of sperm morphology in male Mytilus coruscus, which was collected on the coastal waters of Gyeokpo in western Korea, were investigated by transmission electron microscope observations. The morphology of the spermatozoon has a primitive type and is similar to those of other bivalves in that it contains a short midpiece with five mitochondria surrounding the centrioles. The morphologies of the sperm nucleus type and the acrosome shape of this species have an oval and modified cone shape, respectively. In particular, the axial rod is observed between the nucleus and acrosome of the sperm. The spermatozoon is approximately $45-50{\mu}m$ in length including a sperm nucleus (about $1.46{\mu}m$ in length), an acrosome (about $3.94{\mu}m$ in length) and tail flagellum (approximately $40-45{\mu}m$). The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure. Some special charateristics of sperm morphology of this species in the genus Mytilus are (1) acrosomal morphology, (2) the number of mitochondria in the midpiece of the sperm, and (3) the existence of a satellite. The axial rod appears in the acrosome and sperm nucleus as one of the characteristics seen in several species of the subclass Pteriomorphia, unlikely the subclass Heterodonta containing axial filament instead of the axial rod. The number of mitochondria in the midpiece of the sperm of this species in the family Mytilidae are five, as one of common characteristics appeared in most species in the family Mytilidae. Most of Mytilus species contain a satellite body which is attached to the proximal centriole in the middle piece of the sperm, as one of common characteristics of sperm morphology in the family Mytilidae.

FLOER MINI-MAX THEORY, THE CERF DIAGRAM, AND THE SPECTRAL INVARIANTS

  • Oh, Yong-Geun
    • Journal of the Korean Mathematical Society
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    • v.46 no.2
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    • pp.363-447
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    • 2009
  • The author previously defined the spectral invariants, denoted by $\rho(H;\;a)$, of a Hamiltonian function H as the mini-max value of the action functional ${\cal{A}}_H$ over the Novikov Floer cycles in the Floer homology class dual to the quantum cohomology class a. The spectrality axiom of the invariant $\rho(H;\;a)$ states that the mini-max value is a critical value of the action functional ${\cal{A}}_H$. The main purpose of the present paper is to prove this axiom for nondegenerate Hamiltonian functions in irrational symplectic manifolds (M, $\omega$). We also prove that the spectral invariant function ${\rho}_a$ : $H\;{\mapsto}\;\rho(H;\;a)$ can be pushed down to a continuous function defined on the universal (${\acute{e}}tale$) covering space $\widetilde{HAM}$(M, $\omega$) of the group Ham((M, $\omega$) of Hamiltonian diffeomorphisms on general (M, $\omega$). For a certain generic homotopy, which we call a Cerf homotopy ${\cal{H}}\;=\;\{H^s\}_{0{\leq}s{\leq}1}$ of Hamiltonians, the function ${\rho}_a\;{\circ}\;{\cal{H}}$ : $s\;{\mapsto}\;{\rho}(H^s;\;a)$ is piecewise smooth away from a countable subset of [0, 1] for each non-zero quantum cohomology class a. The proof of this nondegenerate spectrality relies on several new ingredients in the chain level Floer theory, which have their own independent interest: a structure theorem on the Cerf bifurcation diagram of the critical values of the action functionals associated to a generic one-parameter family of Hamiltonian functions, a general structure theorem and the handle sliding lemma of Novikov Floer cycles over such a family and a family version of new transversality statements involving the Floer chain map, and many others. We call this chain level Floer theory as a whole the Floer mini-max theory.

Germ cell Differentiation During Spermatogenesis, and Ultrastructural Characteristics of Mature Sperm in Male Phacosoma japonicus (Bivalvia: Veneridae)

  • Kim, Jin-Hee;Chung, Ee-Yung;Choi, Moon-Sul;Lee, Ki-Young;Lee, IL-Ho;Seo, Won-Jae
    • The Korean Journal of Malacology
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    • v.27 no.1
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    • pp.55-65
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    • 2011
  • Some characteristics of germ cell differntiations during spermiogenesis and mature sperm ultrastructure in male Phacosoma japonicus were investigated by transmission electron microscope observations. The morphology of the spermatozoon of this species has a primitive type and is similar to those of other species in the subclass Heterodonta. Morphologies of the sperm nucleus and the acrosome of this species are the cylindrical type and cap shape, respectively. The spermatozoon is approximately 45-50 ${\mu}m$ in length, including a long curved sperm nucleus (about $3.70{\mu}m$ long with 45 $^{\circ}$ of the angle of the nucleus, an acrosome (about $0.55{\mu}m$ in length), and tail flagellum (about 42-$47{\mu}m$)The axoneme of the sperm tail shows a 9+2 structure. As some characteristics of the acrosomal vesicle structures, the basal and lateral parts of basal rings show electron opaque part (region), while the anterior apex part of the acrosomal vesicle shows electron lucent part (region). These characteristics of the acrosomal vesicle were found in the family Veneridae and other several families in the subclass Heterodonta. These common characteristics of the acrosomal vesicle in the subclass Heterodonta can be used for phylogenetic and systematic analysis as a taxonomic key or a significant tool. The number of mitochondria in the sperm midpiece of this species are four, as one of common characteristics appear in most species in the family Veneridae and other families in the subclass Heterodonta. However, exceptionally, only three species in Veneridae of the subclass Heterodonta contain 5 mitochondria. The number of mitochondria in the sperm midpiece can be used for the taxonomic analysis of the family or superfamily levels as a systematic key or tools.

Analysis of Cross-correlation Frequency between Non-linear Binary Sequences Family with 5-Valued Cross-Correlation Functions (5-값 상호상관관계를 갖는 비선형 이진수열군의 상호상관관계 빈도 분석)

  • Choi, Un-Sook;Cho, Sung-Jin
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.12
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    • pp.2875-2882
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    • 2013
  • The design of PN(Pseudo Noise) sequences with good cross-correlation properties is important for many research areas in communication systems. Also analyses of cross-correlation frequency between designed sequences have been researched. In this paper we analyze of cross-correlation distribution and properties of non-linear binary sequences family $S^r=\{Tr^m_1\{[Tr^n_m(a{\alpha}^t+{\alpha}^{dt}]^r\}{\mid}a{\in}GF(2^m),0{\leq}t < 2^n-1\}$, where $gcd(r,2^m-1)=1$ with 5-valued cross-correlation.

The anti-cancer effects of $Ampelopsisradix$ Extract (AE) on A549 cells - The role of Bcl-2 family protein on the AE-induced apoptosis - (폐암세포에서 백렴의 항암효능연구 - Bcl-2 family 단백조절을 통한 자가사멸 -)

  • Nam, Hye-Seon;Cho, Min-Kyung
    • Journal of Society of Preventive Korean Medicine
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    • v.16 no.1
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    • pp.71-80
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    • 2012
  • Objective : The aim of this study is to evaluate anti-cancer effects of $Ampelopsisradix$ Extract (AE) on human lung cancer A549 cells. Method : The apoptotic activities and cell growth arrest activities of AE were measured using 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The molecules involved in apoptotic process were assessed by western blotting. Result : Treatment of AE potently reduced cell viability in a dose-dependent manner in A549 cells. AE (100-500 ${\mu}g/m{\ell}$) resulted in apoptosis via activation of caspase 9 following PARP cleavage in a time-and dose-dependent manner. The levels of Bax and Bad levels were increased by AE with a concomitant decrease of Bcl-xL. In addition, AE at the low dose (30 ${\mu}g/m{\ell}$) significantly inhibited cell growth in the presence of serum. Conclusion : AE has the potential as a therapeutic agent against lung cancer.

Social Authority as Perceived by Children and Adolescents Removed from Illegally Built Shacks (철거촌 아동과 청소년의 권위 대상에 대한 인지)

  • Yi, Soon Hyung
    • Korean Journal of Child Studies
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    • v.13 no.1
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    • pp.65-79
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    • 1992
  • The present study investigated perception of social authority by children and adolescents removed from illegally built shacks 181 children and adolescents from illegally built shacks and 200 comparative respondants of middle socioeconomic status were investigated. Perception of the social authority figures was classified by 5 categories(self, family member, neighbor, teacher and peer. social authority, collective member) in terms of contactable possibility and psychological distance. The data were analyzed by Hest. The results showed that (1) compared with their middle class peers(M group) children and adolescents from illegally built shacks(S group) perceived social authority figures positively, while they perceived their own family members less favorably. (2) The S group regarded their teachers as more valuable and able than M group. (3) The S group perceived social authority figures, i. e., statemen. polices. national assembly members. public servants and enterprisers, less favorably than their family members. (4) There were main effects of the age and sex variables of respondants. (5) There was an interaction effects between the age. sex and SES variables and was also interation effect between age. sex and task variables in cognition for the social authority figures. There was also an interaction effect between age and SES variables in perception for the personal traits of the social authority figures.

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Spermiogenesis and Taxonomic Value of Sperm Morphologies of Two Species in Veneridae (Bivalvia: Heterodonta)

  • Kim, Jin-Hee;Kim, Sung-Han
    • The Korean Journal of Malacology
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    • v.27 no.2
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    • pp.149-157
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    • 2011
  • Some characteristics of the formations of acrosomal vesicles during the late stage of spermatids during spermiogenesis and taxonomical charateristics of sperm morphology in male two species (Saxidomus purpurata and Meretrix petechialis) in the family Veneridae were investigated by electron microscope observations. In two species, the morphologies of the spermatozoa have the primitive type and are similar to those of other bivalves in that it contains a short midpiece with five mitochondria surrounding the centrioles. The morphologies of the sperm nuclear types of S. purpurata and M. petechialis in Veneridae have the curved cylindrical and cylinderical type, respectively. And the acrosome shapes of two species are the same cap-shape type. In particular, the axial filament is not found in the lumen of the acrosome of two species, however, subacrosomal material are observed in the subacrosomal spaces between the anterior nuclear fossa and the acrosomal vesicle of two species. The spermatozoon of S. purpurata is approximately 46-$52{\mu}m$ in length, including a curved sperm nucleus (about $3.75{\mu}m$ in length), a long acrosome (about $0.40{\mu}m$ in length),and a tail flagellum (about 45-$47{\mu}m$ long). And the spermatozoon of M. petechialis is approximately 47-$50{\mu}m$ in length including a slightly curved sperm nucleus (about $1.50{\mu}m$ in length), an acrosome (about $0.56{\mu}m$ in length) and tail flagellum (44-$48{\mu}m$ in length). In two species, the axoneme of the sperm tail flagellum of each species consists of nine pairs of microtubules at the periphery and a pair of cental doublets at the center. Therefore, the axoneme of the sperm tail flagellum shows a 9 + 2 structure. In particular, taxonomically important some charateristics of sperm morphologies of two species in the family Veneridae are acrosomal morphology of the sperm, The axial filament is not found in the acrosome as seen in a few species of the family Veneridae in the subclass Heterodonta. The acrosomal vesicle is composed of right, left basal rings and the apex part of the acrosomal vesicle. In particular, right and left basal rings show electron opaque part (region), while the apex part of the acrosomal vesicle shows electron lucent part (region). These charateristics belong to the subclass Heterodonta, unlikely a characteristic of the subclass Pteriomorphia showing all part of the acrosome being composed of electron opaque part (region). Therefore, it is easy to distinguish the families or the subclasses by the acrosomal structures. The number of mitochondria in the midpiece of the sperm of S. purpurata and M. petechialis in Veneridae are five. However, the number of mitochondria in the midpiece of the sperm in most species of Veneridae in the subclass Heterodonta are four. Therefore, the number of mitochondria of the sperm midpiece of two species are exceptionally 5, and it is only exceptional case in the species in Veneridae in the subclass Heterodonta. Except these cases, the number of mitochondria in the sperm midpiece in all families in the subclass Heterodontaare are 4, and now widely used in taxonomic analyses.

Study on Genetic Evaluation using Genomic Information in Animal Breeding - Simulation Study for Estimation of Marker Effects (가축 유전체정보 활용 종축 유전능력 평가 연구 - 표지인자 효과 추정 모의실험)

  • Cho, Chung-Il;Lee, Deuk-Hwan
    • Journal of Animal Science and Technology
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    • v.53 no.1
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    • pp.1-6
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    • 2011
  • This simulation study was performed to investigate the accuracy of the estimated breeding value by using genomic information (GEBV) by way of Bayesian framework. Genomic information by way of single nucleotide polymorphism (SNP) from a chromosome with length of 100cM were simulated with different marker distance (0.1cM, 0.5cM), heritabilities (0.1, 0.5) and half sibs families (20 heads, 4 heads). For generating the simulated population in which animals were inferred to genomic polymorphism, we assumed that the number of quantitative trait loci (QTL) were equal with the number of no effect markers. The positions of markers and QTLs were located with even and scatter distances, respectively. The accuracies of estimated breeding values by way of indicating correlations between true and estimated breeding values were compared on several cases of marker distances, heritabilities and family sizes. The accuracies of breeding values on animals only having genomic information were 0.87 and 0.81 in marker distances of 0.1cM and 0.5cM, respectively. These accuracies were shown to be influenced by heritabilities (0.87 at $h^2$ =0.10, 0.94 at $h^2$ =0.50). According to half sibs' family size, these accuracies were 0.87 and 0.84 in family size of 20 and 4, respectively. As half sibs family size is high, accuracy of breeding appeared high. Based on the results of this study it is concluded that the amount of marker information, heritability and family size would influence the accuracy of the estimated breeding values in genomic selection methodology for animal breeding.