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Effects of soluble silicon on development powdery mildew(Sphaerotheca fuliginea) in cucumber plants (규소 시용에 의한 오이 흰가루병 발병억제)

  • Lee, Jung-Sup;Yiem, Myeong-Sun
    • The Korean Journal of Pesticide Science
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    • v.4 no.2
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    • pp.37-43
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    • 2000
  • Effects of silicon application on development of colonies of Sphaerotheca fuliginea were examined. Cucumber plants were applied with nutrient solutions amended with different concentrations of soluble silicon and selected leaves were inoculated with known concentrations of conidia of the pathogen. Colony number per leaf, colony area per leaf, and germination rate of conidia of S. fuliginea collected from the inoculated leaves were reduced as silicate concentrations in the nutrient solutions increased from 0.05 to 4.10 mM. The increase in resistance of plants to mildew infection was apparently due to silicate accumulation in leaves, and there was no correlation between cation or ionic strength effects and the silicate treatments. Silicate treatment in growth medium remarkably suppressed powdery mildew development on cucumber. Colonies of mildew fungus were visible with over approx. 38.3% of the mature leaf surface, while that of the leaves in high Si plants was 2.3% observed at 51 days after transplanting. No significant differences were observed between 1.7 mM and 3.4mM silicate treatments. Conidial germination rates were significantly reduced by increasing Si amendments. Conidial germination ranged from 14.7 to 20.3% for plants grown in low Si solution(<1.40 mM), and from 9.0 to 12.4% for plants grown in high Si solution(>1.8 mM). Foliar applications of Si with ${\geq}$ 17.0 mM decreased the number of powdery mildew colonies. Persistence of Si foliar sprays effects on cucumber demonstrated that the 17 mM Si spray applied 4 days before inoculation with S. fuliginea reduced mildew colony formation. The relationship was positive and linear.

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Studies on the Spore Formation of Aspergillus niger in Potassium Acetate Medium (아세트산염 배지에서의 검정곰팡이(Aspergillus niger)의 포자형성에 관한 연구)

  • Lee, Ho-Young;Kim, Jong-Hyup
    • The Korean Journal of Mycology
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    • v.15 no.3
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    • pp.149-157
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    • 1987
  • This study was undertaken to investigate the differentiation, from spore germination to hyphae growth and phialide formation, of Aspergillus niger through the method of synchronous and submerged culture. Through continuous experiments by shake culture with potassium acetate medium, we observed the formation of spores at appropriate concentration and pH. Potassium acetate medium was set pH 5.5, 6.0, 6.5 and 7.0 on each scale, and control, 20 mM, and 40 mM, 80 mM and 160 mM concentrations on the other scale. Aspergillus niger was cultured in the defined media at $28^{\circ}C$, and mycelial dry weight, changes of pH and the onset of sporulation were checked. The mycelial dry weight, increased in potassium acetate medium, and pH increased during mycelial growth and gradually decreased after the spore formation. When pH increased excessively in Potassium acetate medium with pH 7.0, the mycelia could not adapt and mycelial dry weight decreased gradually. At pH 5.5, the onset of sporulation was done within one day at 20 mM it took, at 80 mM three days and at 160 mM concentration. in two days, at 40 mM one to four days were taken, 80 mM concentration respectively. At pH 6.5, the onset of sporulation was done in three days and four days at 80 mM concentrations respectively. Spore formation was not shown at pH 7.0. In controlled medium with all levels of pH, spore formation was not shown.

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The Analysis of Fractal Dimension on the Main-Stream Length using Digital Elevation Model (수치고도모형을 이용한 본류하천길이에 관한 프랙탈 차원의 해석)

  • 차상화
    • Journal of Environmental Science International
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    • v.11 no.9
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    • pp.915-923
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    • 2002
  • Recently, GIS(Geographic Information System) is used to extract various hydrological factors from DEM(Digital Elevation Model) in river basin. Therefore, this study aims at the determination of river fractal dimension using DEM. In this paper, the main-stream length in river basin was grid-analyzed for each scale(1/5,000, 1/25,000, 1/50,000) and each cell size(5m$\times$5m, l0m$\times$l0m, 20m$\times$20m, 30m$\times$30m, 40m$\times$40m, 50m$\times$50m, 60m$\times$60m, 70m$\times$70m, 80m$\times$80m, 90m$\times$90m, 100m$\times$l00m, 120m$\times$120m, 150m$\times$150m) using GIS. Also, fractal dimension was derived by analyzing correlation among main-stream lengths, scale, and cell size which were calculated here. The result of calculating fractal dimension for each cell size shows that the fractal dimension on the main-stream length is 1.028.

An Exploratory Study on the Feasibility of M2M Services (사물통신(M2M) 기반 응용 서비스의 사업성에 관한 탐색적 연구)

  • Lee, Ji-Eun;Shin, Min-Soo
    • Journal of Information Technology Services
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    • v.11 no.1
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    • pp.125-139
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    • 2012
  • Machine-to-Machine(M2M) refers to technologies allowing both wireless and wired systems to communicate with other devices. The enhancement of wireless technologies and the reduced cost of wireless connection have contributed toward the development of M2M market. M2M market currently focuses on public sector services, As M2M can offer scalable and flexible services, M2M services are expected to be applied in various fields. However there are still some doubts on whether the demand for M2M services will be increased. This study investigates the feasibility of M2M services from the perspective of operators. Operators evaluate that M2M services will show high marketability but low profitability in general. In addition, operators predict that M2M services will show both marketability and profitability in the area of car maintenance, insurance, telematics, home and office security, shop floor maintenance, and real-time locating services for children protection. This study may offer some ideas of M2M business models based on empirical feasibility studies.

Towards Agile Application Integration with M2M Platforms

  • Chen, Menghan;Shen, Beijun
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.6 no.1
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    • pp.84-97
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    • 2012
  • M2M (Machine-to-Machine) Technology makes it possible to network all kinds of terminal devices and their corresponding enterprise applications. Therefore, several M2M platforms were developed in China in order to collect information from terminal devices dispersed all over the local places through 3G wireless network. However, when enterprise applications try to integrate with M2M platforms, they should be maintained and refactored to adapt the heterogeneous features and properties of M2M platforms. Moreover, syntactical and semantic unification for information sharing among applications and devices are still unsolved because of raw data transmission and the usage of distinguished business vocabularies. In this paper, we propose and develop an M2M Middleware to support agile application integration with M2M platform. This middleware imports the event engine and XML-based syntax to handle the syntactical unification, makes use of Ontology-based semantic mapping to solve the semantic unification and adopts WebService and ETL techniques to sustain multi-pattern interactive approach, in order to agilely make applications integrated with the M2M platform. Now, the M2M Middleware has been applied in the China Telecom M2M platform. The operation results show that applications will cost less time and workload when being integrated with M2M platform.

A Study about Development of Hydrogen Peroxide Stabilizer in Modified Fenton Reaction Using Anion Surfactant (음이온 계면활성제를 사용한 modified Fenton 반응의 과수안정제 개발에 관한 연구)

  • Kim, Han Ki;Park, Kang Su;Kim, Jeong Hwan;Park, Joo Yang
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.31 no.4B
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    • pp.377-382
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    • 2011
  • In this study, hydrogen peroxide is stabilized in modified Fenton reaction to improve the soil remediation. Phenanthrene, which is the typical compound in PAHs, was spiked into soil samples to copy the original contaminated site. Anionic surfactant, SDS (Sodium dodecyl sulfate) was used for hydrogen peroxide stabilizer. 4 mM of Fe(II), 5~50 mM of SDS and 102.897 mM of $H_2O_2$ was injected into soil samples which is contaminated by 125 mg/kg of phenanthrene to analyze decomposition rate of phenanthrene in modified Fenton reaction. In condition which SDS was injected 30 mM, decomposition rate of phenanthrene has best efficiency as 95% and in condition which SDS was injected over 30 mM, decomposition rate is lower than SDS 30 mM because SDS enacted as scavenger in the system. Results which assess the change of hydrogen peroxide concentration after injecting hydrogen peroxide stabilizer showed that hydrogen peroxide concentration was 14.6995 mM so that is stabilized at Fe(II) 2 mM condition in 48 hours. On the other hand, hydrogen peroxide is not stable in Fe(III) condition. SDS concentration was fixed and iron concentration was changed 2~8 mM to find out optimize proportion between iron concentration and SDS concentration in modified Fenton reaction. Consequentially, in condition of which Fe(II) 4 mM and SDS 30 mM, reaction has the highest removal rate as 95%.

Determination of Microquantities of Ammonia by Enzymatic Analysis (효소분석법에 의한 미량암모니아의 정량)

  • 성하진;양한철
    • Microbiology and Biotechnology Letters
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    • v.14 no.6
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    • pp.495-500
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    • 1986
  • Enzymatic micro-assay methods were studied those were capable of determining ammonia down to 10$^{-5}$M(0.01 $\mu$mole/ml) in the presence of other nitrogenous compounds such as protein and amino acid. Microquantities of ammonia (0.01-0.1 $\mu$mole) could be determined indirectly by measuring phosphorous, one of the products of the enzymatic reaction catalyzed by glutamine synthetase. In this reaction, L-glutamate, ATP and ammonium chloride were used as substrates, and phosphorous was formed in propotion to the concentration of ammonium chloride In the reaction mixture. Another procedure was examined in which glutamine synthetase reaction coupled with pyruvate kinase and lactate dehydrogenase reactions was used. One mililiter of the assay mixture contained; phosphoenol pyruvate, 3 mM, L-glutamate, 10 mM; ATP, 1mM: MgSO$_4$, 20 mM: KCl, 75mM: NADH, 0.2mM: Tris-HCl buffer(pH 7.0), 100mM; pyruvate kinase, 10 U: lactate dehydrogenase, 12 U and glutamine synthetase, 4 U. After preincubation for 20 min at 3$0^{\circ}C$, NH$_4$Cl was added and the rates of NADH oxidation were followed at 340nm. The effective range of this method was proved to be from 0.01 to 0.05 $\mu$mole/$m{\ell}$. Glutamine synthetase reaction coupled with glutamate synthase reaction could also be effectively used for determining microquantities of ammonia. The one mililiter assay mixture contained; ATP, 5mM: L-glutamate, 5mM; L-ketoglutarate, 5mM; MgCl$_2$, 15mM; NADPH, 0.15mM; Tris-HCl buffer(pH 7.0); 100mM; glutamine synthetase, 1U and glutamate synthase, 0.5U. After preincubation for 20min at 3$0^{\circ}C$ NH$_4$Cl was added and the rates of NADPH oxidation were followed at 340nm. The effective range of this procedure was appeared to be from 0.01 to 0.05$\mu$mole/$m{\ell}$.

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The Combination Effect of Kinetin and Auxin on the Growth Root Development and Vitamin C Content of Soybean sprouts (콩나물의 성장과 Vitamin C 생성에 미치는 Kinetin과 Auxin의 혼합효과)

  • Kim, Sang-Ock
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.11 no.2
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    • pp.37-41
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    • 1982
  • This study was conducted to investigate the growth of soybean sprouts and the effective hormonal refutation of vitamin C content by the concentration and combination of kinetin and auxins such as IAA, 2.4-D and NAA. The results were as follows; 1. The growth of soybean sprouts were fine under the respective conditions of kinetin $10^{-8}M+IAA\;10^{-6}M$, kinetin $10^{-7}M+IAA\;10^{-5}M$, kinetin $10^{-6}M+IAA\;10^{-6}M$, kinetin $10^{-5}M+IAA\;10^{-7}M$, kinetin $10^{-8}M+2,4-D\;10^{-8}M$ and kinetin $10^{-7}M+NAA\;10^{-5}M$. 2. The root development of soybean sprouts were almost in accord with the growth of soybean sprouts by the concentration of kinetin and auxin. 3. The content of vitamin C were more increased under in kinetin $10^{-8}M+IAA\;10^{-8}M$, kinetin $10^{-6}M+2,4-D\;10^{-5}M$ and kinetin $10^{-8}M+NAA\;10^{-8}M$ and both growth and vitamin C content were more activated under kinetin $10^{-8}M+IAA\;10^{-6}M$ and $10^{-8}M+NAA\;10^{-8}M$. 4. The growth, root development and vitamin C content were increased in the low kinetin and high IAA, NAA concentration. Root development and vitamin C content of soybean hypocotyl were decreased in the low kinetin and high 2,4-D concentration.

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Treatment Characteristics of Trichloroethylene(TCE) by Oxidation and Reduction with Nanoscale Zero-valent Iron (나노영가철의 산화·환원에 의한 트리클로로에틸렌 처리특성)

  • Park, Young-Bae;Jung, Yong-Jun;Choi, Jeong-Hak;Moon, Boung-Hyun
    • Journal of Environmental Science International
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    • v.23 no.5
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    • pp.903-910
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    • 2014
  • This study examined the treatment characteristics of hard-to-degrade pollutants such as TCE which are found in organic solvent and cleaning wastewater by nZVI that have excellent oxidation and reduction characteristics. In addition, this study tried to find out the degradation characteristics of TCE by Fenton-like process, in which $H_2O_2$ is dosed additionally. In this study, different ratios of nZVI and $H_2O_2$, such as 1.0 mM : 0.5 mM, 1.0 mM : 1.0 mM, and 1.0 mM : 2.0 mM were used. When 1.0 mM of nZVI was dosed with 1.0 mM of $H_2O_2$, the removal efficiency of TOC was the highest and the first order rate constant was also the highest. When 1mM of nZVI was dosed with 0.5 mM of $H_2O_2$, the first order rate constant and removal efficiency were the lowest. The size of first order rate constant and removal efficiency was in the order of nZVI 1.0 mM : $H_2O_2$ 1.0 mM > nZVI 1.0 mM : $H_2O_2$ 2.0 mM > nZVI 1.0 mM : $H_2O_2$ 0.5 mM > $H_2O_2$ 1.0 mM > nZVI 1.0 mM. It is estimated that when 1.0 mM of nZVI is dosed with 1.0 mM of $H_2O_2$, $Fe^{2+}$ ion generated by nZVI and $H_2O_2$ react in the stoichiometric molar ratio of 1:1, thus the first order rate constant and removal efficiency are the highest. And when 1.0 mM of nZVI is dosed with 2.0 mM of $H_2O_2$, excessive $H_2O_2$ work as a scavenger of OH radicals and excessive $H_2O_2$ reduce $Fe^{3+}$ into $Fe^{2+}$. As for the removal efficiency of TOC in TCE by simultaneous dose and sequential dose of nZVI and $H_2O_2$, sequential dose showed higher first order reaction rate and removal efficiency than simultaneous dose. It is estimated that when nZVI is dosed 30 minutes in advance, pre-treatment occurs and nanoscale $Fe^0$ is oxidized to $Fe^{2+}$ and TCE is pre-reduced and becomes easier to degrade. When $H_2O_2$ is dosed at this time, OH radicals are generated and degrade TCE actively.

The Effects of Branched Chain Amino Acids and Small Metabolites on the Biosynthesis of Acetolactate Synthase in Serratia rnarcescens ATCC 25419 (Branched Chain 아미노산과 대사산물들이 Serratia marcescens ATCC 25419 Acetolactate Synthase의 생합성에 미치는 영향)

  • 최병범;김승수
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.115-121
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    • 1992
  • The effects of branched chain amino acids and small metabolites in growth media on the biosynthesis of Serratia marcescens ATCC 25419 acetolactate synthase (ALS) were examined. ALS activ~ty was gradually decreased by isoleucme or leucine among the range from 1 mM to 20 rnM, while the activity was increased 40% by isoleucine under low concentration (0.5 mM). ALS activity was also increased about 40% by valine among 2 to 4 mM ranges, but the activity was decreased only 10% at 20 mM. ALS activity was decreased 25% and 70% by the simultaneous addition of all three branched chain amino acids at 2 mM and 10 mM, respectively. Among several small metabolites tested, ALS activity was increased about 2-fold by cAMP at 2 mM. These data suggest that Sorrtrtiti rnorcewns ALS is muitivalently repressed by branched chain amino acids, but not repressed by valine alone.

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