• 한국식품과학회지
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• 제21권4호
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• pp.556-561
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• 1989

DSC를 이용하여 쌀에 존재하는 amylose-lysolecithin complex의 열특성을 측정하고 국내에서 재배되는 다수계 및 일반계 쌀 각각 5품종의 amylose 함량을 정량하였다. DSC pan상에서 쌀가루에 충분한 양의 lysolecithin을 첨가하여 amylose-lysolecithin complex가 충분히 생성되도록 한 후 다시 2차 가열하여 complex의 용융현상을 측정하였다. Complex의 용융온도는 $108.5^{\circ}C$부근이었으며 용융엔탈피는 1.0cal/g starch였다. 이 용융엔탈피로부터 amylose 함량을 계산한 결과 16-19%였으며 일반계 및 다수계 쌀간에는 큰 차이가 없었다. 이 값은 비색법에 의해 측정된 값과 비교적 잘 일치하였다. 쌀 전분의 호화온도는 lysolecithin 첨가에 의해 영향받지 않았으나 호화엔탈피는 감소하였다.

• 한국가금학회지
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• 제42권2호
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• pp.133-137
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• 2015

본 연구는 저 에너지 사료 내 lysolecithin과 sodium stearoyl-2-lactylate의 첨가가 육계의 생산성 및 영양소 소화율에 미치는 영향을 규명하였다. 본 시험은 1일령 ROSS 308(♂, ♀) 768수를 공시하였고, 시험 개시 체중은 44.3 g으로 35일간 진행하였으며, 시험설계는 1) PC(basal diet), 2) NC(PC-100 kcal), 3) T1(NC+ 0.08% lysolecithin) and 4) T2(NC + 0.04% sodium stearoyl-2-lactylate)로 4개 처리를 하여 처리당 12반복, 반복당 16수씩 완전 임의 배치하였다. 증체량(body weight gain: BWG), 사료섭취량(feed intake: FI) 및 사료요구율(feed conversion ratio: FCR)은 매주 측정하였다. 산화크롬(Cr2O3)을 표시물로서 0.2% 첨가하여 실험 실험종료 7일 전에 급여하였다. 1~21일차 생산성에 있어 처리구간 유의적인 차이가 나타나지 않았고(P>0.05), 21~35일차 증체량에 있어서 T1 처리구 및 T2 처리구가 대조구와 비교하였을 때 유의적으로 높게 나타났다(P<0.05). 영양소 소화율에 있어 T1 처리구 및 T2 처리구가 대조구와 비교하였을 때 유의적으로 효과가 있었다(P<0.05). 그러나 건물 소화율에 있어서는 처리구간 유의적인 차이를 나타내지 않았다(P>0.05). 종합적으로, 육계 사료 내 유화제의 첨가가 후반 성장 단계에서 생산성, 에너지 소화율 및 질소 소화율을 향상시켰다.

• 한국식품과학회지
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• 제26권2호
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• pp.117-122
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• 1994

여러가지 전분에서 AL-complex의 형성에 lysolecithin의 첨가가 미치는 영향과 cyclodextrin의 첨가에 의한 CL-complex 형성효과를 AL-complex 형성과의 관계로 DSC를 이용하여 비교하였다. 옥수수, 밀, 쌀 전분은 모두 $100^{\circ}C$ 전후의 높은 온도에서 endothermic peak을 나타냈으며 2차 가열에서도 그대로 존재하여 AL-complex의 용융은 가역반응이고 3가지 전분에 상당량의 AL-complex가 존재함을 보여주었다. Lysolecithin의 첨가에 의해 AL-complex의 용융엔탈피는 증가하고 용융온도도 증가하는 경향을 보여 lysolecithin에 의한 AL-complex 형성효과가 뚜렷하였다. 형성된 AL-complex에 ${\beta}-CD$을 첨가하면 AL-complex의 peak가 감소하는 반면 $70^{\circ}C$ 근처에서 새로운 peak가 나타났으며 ${\beta}-CD$의 농도를 증가하였을 때 AL-complex의 peak가 비례하여 감소하였다. 이는 $lysolecithin-{\beta}-CD$ complex가 형성되어 lysolecithin이 ${\beta}-CD$으로 전이되었으며 동시에 amylose가 complex로부터 유리되었음을 시사하고 있다. 이를 증명하기 위하여 ${\beta}-CD$의 존재하에 AL-complex를 가열한 후 amylase를 착용시킨 결과 가수분해속도가 현저히 증가하여 AL-complex로부터 amylose가 유리됨을 알 수 있었다.

• 공업화학
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• 제30권4호
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• pp.487-492
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• 2019

주름 개선을 위한 활성물질인 아데노신의 경피 투과를 위해 나노 플렉시블 베시클에 포집을 시도하였다. 나노 플렉시블 베시클은 인지질, 에탄올, lysolecithin으로 구성되는데, 수화 과정에서 형성된 액정 상을 물속에 분산시켜 만드는 액정형 베시클이다. 본 연구에서는 베시클 입자크기에 영향을 미치는 요인을 알아보기 위하여 실험계획법 중 하나인 다구찌 방법을 적용하였다. 다구찌 직교 배열을 활용하여 베시클 입자크기에 대한 망소 특성의 S/N 비를 산출하였다. 베시클 구성성분에서 에탄올과 lysolecithin 비율, 수화 과정에서 투입되는 수용액 양 등이 베시클 입자크기에 큰 영향을 미치는 주요 인자들이고, ANOVA 분석을 통해 이들 인자가 신뢰수준 95%에서 유의함을 확인하였다.

• 한국가축번식학회지
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• 제10권2호
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• pp.192-203
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• 1986

Two experiments in this study were designed to compare the potential for in vitro capacitation and in vitro fertilization of ejaculated sperm among individual rabbit bucks. In experiment 1, for in vitro capacitation, the ejaculated sperm were preincubated in DM for 12 hr or 18 hr after HIS treatment, then 12 hr -or 18 hr- preincubated sperm were incubated with superovulated rabbit ova in a 5% CO2 incubator for 36 hr at 38$^{\circ}C$, and a part of cleaved ova was transferred to the recipient does for implantation of embryo. In experiment 2, effect of lysolecithin addition to preincubation medium on induction of accelerated in vitro capacitation and in vitro fertilization of individual rabbit sperm was studied. Experiment 1; 1. Percent acrosome reaction of sperm, noted after staining, after 12 hr or 18 hr preincubation ranged from 52.5 to 76.0% and from 67.5 to 90.0%, respectively and sperm motility index of these sperm ranged from 20.0 to 47.5 for 12 hr-preincubated sperm and from 15.0 to 37.5 for 18 hr- preincubated sperm. There was no a certain relation between percent acrosome reaction and sperm motility index. 2. In vitro fertilization rate (cleavage rate) of in vitro capacitated sperm varied widely among individual bucks, ranging from 0 to 47.8% for 12 hr - preincubated sperm and from 0 to 60.9% for 18 hr -prein- cubated sperm. Cleavage rate of 18 hr - preincubated sperm was higher and faster than that of 12 hr - preincubated sperm. 3. Eight of 44 in vitro fertilized embryos transferred into 6 recipients were implanted in 4 recipients (66.7%) up to day 15 and implnatation rate was 18.2%. Experiment 2; 1. The percent acrosome reaction of sperm before and after 4 hr preincubation in DM without lysolecithin varied significantly among individual bucks, ranging from 0.4 to 18.4% and from 1.7 to 37.4%, respectively and percent acrosome reaction of sperm at 30 min after addition of 60${\mu}$g/ml lysolecithin also was significantly different among bucks, ranging from 19.2 to 67.1%. 2. Effect of accelerated acrosome reaction following lysolecithin addition was more considerable in the individuals showed less percent acrosome reaction before and after 4 hr preincubation. Percentage of motile sperm and motility score showed a trendency towards a decrease with increase of preincubation time and time after lysolecithin addition. 3. In vitro fertilization rate (cleavage rate) at 24 hr postinesmination with pooled sperm were treated to 60 $\mu\textrm{g}$/ml lysolecithin for 30 min after 4 hr preincubation was 24.6%, a higher rate than 13.2% for control. While 80 $\mu\textrm{g}$/ml lysolecithin-added sperm showed a lower cleavage than control and 60$\mu\textrm{g}$/ml-added sperm at both 24 hr and 48 hr postinsemination. These results from 2 experiments suggest that more useful preincubation time for the in vitro capacitation of ejaculated rabbit sperm is 18 hr in DM after HIS treatment, although there is wide variation in vitro capacitation and in vitro fertilization rate among individual bucks, and lysolecithin addition to at least 4 hr - preincubated sperm in DM can result in almost same in vitro fertilization rate as that of 18 hr - preincubated sperm in the experiment 1.

• 공업화학
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• 제28권6호
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• pp.679-684
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• 2017

베시클 막을 유연하게 만드는 edge activator를 혼합하여 수화 액정형 베시클을 제조하고 niacinamide를 베시클 안에 포집시켰다. 제조 과정 중 액정 상 형성 및 액정의 열적 상전이 현상을 편광현미경과 시차주사 열량계(DSC)를 통해 살펴보았다. Sodium deoxycholate, lysolecithin, polysorbate 80 등의 edge activator를 첨가하면 수화 액정형 베시클 입자가 수십 나노 사이즈로 줄어들었다. 수화 액정형 베시클을 활용하여 niacinamide를 피부 침투시키면 수용액 상태로 도포했을 때보다 피부 침투된 niacinamide의 양이 크게 증가하는데, 10% sodium deoxycholate를 혼합한 베시클에서는 niacinamide 침투량이 4배 가까이 증가하였다. 이러한 결과로부터 edge activator를 베시클에 혼합하면 베시클의 피부 침투력이 향상됨을 알 수 있었다.

• 대한화장품학회지
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• 제25권1호
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• pp.137-155
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• 1999

천연계면활성제인 lysolecithin과 오일성분인 squalane(SQ.), liquid paraffin(LP), octylpalminate(OP), octylstearate(OS), alkylbenzoate(AB), isostearylbenzoate (ISB)를 사용하여 제조된 에멀젼의 입자의 크기, 형상을 광산란 장치를 사용하여 다음과 같은 결과를 얻었다. 동적광산란실험을 통하여 에멀젼입자는 크기가 150nm~250nm로서 subemulsion 임을 확인하였고, 오일상의 농도가 0.25wt%에서 입자의 크기가 감소하다가 증가하는 오일의 임계농도가 있음을 알 수 있었다. 그리고 SQ에서 ISB의 순으로 오일의 극성이 증가할수록 입자의 크기가 감소함을 알 수 있었다. 정적광산란을 통하여 산란강도의 각도의존성으로부터 관성회전반경(R$_{g}$)를 구할 수 있었고 R$_{g}$/R$_{h}$로부터 극성류인 ISB, AB의 입자형태는 구형, 반극성인 LP, SQ는 타원형 그리고 비극성류인 LP, SQ는 막대형에 가까운 형태임을 알 수 있었다. 제조된 에멀젼의 점도는 구형입자에 비하여 막대형 입자의 점도가 높음을 알 수 있었다.다.다.

• 한국가축번식학회지
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• 제10권1호
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• pp.109-120
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• 1986

This study was conducted to define the effect of addition of lysolecithin (LC) and 20% v/v rabbit serum to sperm preincubation medium on the induction of acrosome reaction (AR) an fertilizing ability in vitro of LG-added sperm. Ejaculated rabbit sperm from New Zealand White buck was washed once by centrifugation, then preincubated for 2 or 4 hrs in a chemically defined medium (DM), DM plus 20% rabbit serum or BSA-free DM plus 20% rabbit serum at 37$^{\circ}C$ water bath or CO2 incubator. At the end of preincubation LC was added to the preincubated sperm, which was stained at 0.5 to 4 hr later and examined for AR and sperm motility. For in vitro fertilization, gametes were coincubated in DM up to 24 hrs and thereafter fertilized embryos were incubated in BSM -II up to 48 hrs. Addition of LC to 4-hr preincubated sperm was more effective for the AR and sperm motility than that to 2-hr preincubated sperm and optimal concentration of LC for AR was about 80${\mu}$g/ml. A significant increase in AR occured from 20 to 30 min. after addition of 80 to 100${\mu}$g/ml in 4-hr preincubated sperm. BSA-free DM plus 20% rabbit serum showed a higher AR and sperm motility than those of DM plus 20% rabbit serum in LC-added sperm after 4-hr preincubation. The incidence of AR after 4-hr preincubation and at 30 min after 60${\mu}$g/ml LC addition varied greatly among individual bucks. Sixty ${\mu}$g/ml LC-added sperm showed a slight high cleavage rate over control levels, but 100${\mu}$g/ml LC-added sperm showed lower cleavage rate rather than 60${\mu}$g/ml LC. It is concluded that optimal concentration of LC for high AR induction and sperm motility in 4-hr preincubated sperm was about 80${\mu}$g/ml, but 60${\mu}$g/ml level was more useful for in vitro fertilization.

• Asian-Australasian Journal of Animal Sciences
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• 제20권1호
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• pp.36-40
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• 2007

Proteins and lipids not only provide a source of energy to the cell, but also play vital roles in modifying the physical properties and function of the biological membranes. In the present study, we investigated the biochemical constituents, viz. proteins and lipids, in growing oocytes of goat antral follicles during summer and winter seasons. Goat genitalia in phosphate buffered saline (pH 7.4) were brought to the laboratory within one hour of slaughter under aseptic conditions at $37^{\circ}C$. Oocytes were aspirated from normal small (<3 mm in diameter) and large (>3 mm) follicles and pooled for biochemical estimations. A significant increase in the amount of protein and lipid was observed with the growth of the oocyte. The amount of protein varied non-significantly with the season, while the amount of lipid varied significantly. The amounts of phospholipid, cholesterol, free fatty acid, and triglyceride increased with the growth of the oocyte, but no significant effect of season in these constituents was observed. Lysolecithin, sphingomyelin, and sterols were the polar lipids identified in both oocytes prepared from small follicles (small oocytes) as well as large follicles (large oocytes). In addition, the small oocytes also contained phosphatidyl serine, while large oocytes contained phosphatidyl glycerol phosphate and phosphatidyl inositol. Among non-polar lipids, triglycerides and long chain alcohols appear only in small oocytes and not in large oocytes. Monoglycerides, 1,2-diglycerides, 1,3-diglycerides and o-dialkyl glycerol ethers, fatty acids, fatty acid methyl esters, and wax esters were identified in both small and large oocytes. Information on biochemical composition of growing oocytes is relevant to oocyte and embryo competence, culture and cryopreservation.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.268-279
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• 2003

The nano capsulation of the ceramide was a technique that capsulated ceramide III and tocopheryl linoleate at the mono-vesicle, so as to act the horny layer in skin. It was used 0.5-5.0 wt% of hydrogenated lecithin and 0.01~2.00 wt% of lysolecithin as the membrane-strengthen agents of the mono-vesicle, 5.0~10 wt% of propylene glycol and 5.0~10.0 wt% of ethyl alcohol made by high-pressure Microfluidizer. To enhance the moisturizing efficacy and treat an atopy skin, used ceramide III and tocopheryl linoleate as the active ingredients, and it was made the nano-capsule that synthetic emulsifiers were free. The optimal condition of capsulation of nano ceramide was as follows. The conditions were 3 times at 1,000bar and 60-7$0^{\circ}C$. The particle size showed 63.1$\pm$7.34 nm such as the transparence water as the results for measuring by the laser light scattering. A zeta potential value was -55.1$\pm$0.84 ㎷. The result of the clinical test, the moisturizing effect (in-vivo, n=8, p-value<0.05) was improved 21.15% compared to control, as well as it was improved 36.31 % before the treatment. Moreover, the effectiveness of atopy skin indicated positive reaction that patients were 10 volunteers.