• Korean Journal of Food Science and Technology
• /
• v.26 no.4
• /
• pp.393-397
• /
• 1994

The study was carried out to develop the powdered smoked-dried anchovy products as a natural flavoring substance. The processing conditions, chemical and taste compounds of products were as follows: The raw anchovy were washed, and then boiled 5 minutes in $5{\sim}6%$ NaCl and 1.0% sodium erythorbate solution. Boiled anchovy were smoked in smoking house at $40^{\circ}C$ for 4 hours as the first stage, and then increased temperature up to $80^{\circ}C$ as the second stage, and finally smoked 8 hrs at $80^{\circ}C$ to maintain the moisture content between 9 and 10 percent. The smoked-dried anchovy were pulverized and screened to be 50 mesh of particle size, and finally packed in PET/Al/CPP film bag. The moisture, crude lipid content and salinity of powdered smoke-dried anchovy were 9.4%, 9.6% and 6.9%, respectively. Fatty acid composition of product was mainly consisted of polyenes (43.4%) such as 22 : 6 and 20 : 5, followed by saturates (36.9%), monoenes (19.7%). The principal taste compounds of product were IMP, 466.5 mg/100g; free amino acids such as His, Tau, Pro, Lys, Ala and Glu, 1179.2 mg/100g; non-volatile organic acids such as lactic acid and succinic acid, 617.9 mg/100g; total creatinine, 595.9 mg/100g; small amount of betaine and TMAO. To make a instant soup, it was desirable for taste of products that powdered smoked-dried anchovy were mixed with 20% salt, 4.0% sugar, 3.0% MSG, 1.0% onion powder, 1.0% garlic powder and 1.0% black pepper.

• Journal of Aquaculture
• /
• v.10 no.1
• /
• pp.87-95
• /
• 1997

This experiment was conducted for compare domestic experimental microparticulated diets with imported commercial microparticulated diets in olive flounder larvae, Paralichthys olivaceus (Temminck et Schlegel). Fish larve were fed four microparticulated diets from 8th day after hatching. Four diets were two commercial microparticulated diets D and H, and experimental microparticulated diets K1 and K2 formulated each with different protein sources (diet K1 ; squid meal, blood meal, yeast extract, chlorella powder, olive flounder muscle, Lys, and Met ; diet K2 ; whole egg protein, krill meal, short-necked clam meal, squid muscle, live yeast, yeast extract, and casein). There were no significant differences on body weight, body length and survival rates among four diet treatments up to the 40th day after hatching. At the 83th day after hatching, fish fed diet D had a significantly higher survival rate than that of fish fed diet K2, whereas there was no significant difference between to diet H and K1. Fish fed diet D had a significantly higher body weight than these of fish fed diaet K1 and K2, whereas there was no significant difference between fish fed diet D and H. There was no significant difference on EPA and DHA of body fatty acid composition among four diet treatments up to the 83th day after hatching. These results show that nutritionally well-ballanced domestic microparticulated diets for olive flouner lavae can be developed.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.4
• /
• pp.596-606
• /
• 2019

N-acyl-homoserine lactone quorum sensing (AHL-QS) has been shown to regulate many physiological behaviors in Serratia marcescens MG1. In the current study, the effects of AHL-QS on the biosynthesis of acid and neutral products by S. marcescens MG1 and its isogenic ${\Delta}swrI$ with or without supplementing exogenous N-hexanoyl-L-homoserine lactone ($C_6-HSL$) were systematically investigated. The results showed that swrI disruption resulted in rapid pH drops from 7.0 to 4.8, which could be restored to wild type by supplementing $C_6-HSL$. Furthermore, fermentation product analysis indicated that ${\Delta}swrI$ could lead to obvious accumulation for acidogenesis products such as lactic acid and succinic acid, especially excess acetic acid (2.27 g/l) produced at the early stage of fermentation, whereas solventogenesis products by ${\Delta}swrI$ appeared to noticeably decrease by an approximate 30% for acetoin during 32-48 h and by an approximate 20% for 2,3-butanediol during 24-40 h, when compared to those by wild type. Interestingly, the excess acetic acid produced could be removed in an AHL-QS-independent manner. Subsequently, quantitative real-time PCR was used to determine the mRNA expression levels of genes responsible for acidogenesis and solventogenesis and showed consistent results with those of product synthesis. Finally, by close examination of promoter regions of the analyzed genes, four putative luxI box-like motifs were found upstream of genes encoding acetyl-CoA synthase, lactate dehydrogenase, ${\alpha}$-acetolactate decarboxylase, and Lys-like regulator. The information from this study provides a novel insight into the roles played by AHL-QS in switching from acidogenesis to solventogenesis in S. marcescens MG1.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.5
• /
• pp.725-730
• /
• 2010

The differences between organic and conventional rice were studied from the point of view of quality, physiochemical property and functionality. It was concluded that organic rice showed a 6% lower complete rice ratio than conventional rice. However, organic rice exhibited more excellent physiochemical property on amylose or protein content, Toyo value, and acidity with 17.5%, 6.6%, 8.12%, and 8.7%, respectively. There was no difference between two kinds of rice on alkali digestion value and amino acids contents such as Asp, Ser, Glu, Ala, Leu, Arg. Among inorganic compounds of rice, four compounds including B, Mn, Fe, or Zn were slightly higher at organic rice with 1.1~2.7 mg/100 g, compared to conventional rice. Interestingly, total phenolic acid and phytic acid content were 89% or 23% higher at organic rice than conventional rice, respectively. MeOH extract from organic rice showed higher antioxidant activity with 26% than that of conventional rice with 22%. In contrast, conventional rice was favored than organic rice on stickness, taste, palatability fields at sensory evaluation.

• Korean Journal of Poultry Science
• /
• v.40 no.4
• /
• pp.361-368
• /
• 2013

This experiment was conducted to determine optimum levels of dietary crude protein for productivity and egg quality in laying hens during early stage. A total of seven hundred and twenty 24-wk-old Hy-Line Variety Brown layers were randomly assigned to 4 experimental diets varying with 16%, 17%, 18%, and 19% CP and fed the diets for 12 wks. There were no significant differences in egg production, daily egg mass and feed intake among experimental diets. Although no difference was found on egg weight among experimental diets, decreasing levels of dietary crude protein tended to reduce the egg weight. Haugh unit and egg shell quality were not affected by different levels of dietary crude protein. Although there was no difference on yolk color among experimental diets, increasing levels of dietary crude protein slightly reduced the yolk color. It is concluded that laying hens did not need more than 16% CP to maximize egg production.

• Journal of Animal Science and Technology
• /
• v.57 no.3
• /
• pp.11.1-11.8
• /
• 2015

An experiment was conducted to evaluate apparent (AID) and standardized (SID) ileal digestibilities of crude protein (CP) and amino acids (AA) with 6 soybean products in weaning pigs. A total of 14 weaning barrows with an initial body weight of $6.54{\pm}0.34kg$ were fitted with T-cannula at the distal ileum and allotted to 7 diets containing various soybean products. The soybean products used in the experiment were conventional soybean meal (CSBM), SBM fermented by Aspergillus oryzae GB-107 (FSBMA), SBM fermented by Bacillus subtilis PP6 (FSBMB), UV sterilized SBM fermented by Bacillus subtilis PP6 (UVFSBMB), SBM containing Bacillus subtilis PP6 (PSBM), and soy protein concentrate (SPC). Six corn-based diets were used and each of soybean products was added. All diets contained 5.0 g/kg of chromic oxide as an indigestible indicator and an N-free diet was used to measure basal endogenous losses of CP and AAs. Ileal CP digestibility did not differ by different soybean products. However, SIDs of Ile, Phe and Val were improved in pigs fed the FSBMB, UVFSBMB and SPC diets and the pigs fed the FSBMA diet showed higher SIDs of Phe and Val compared with those fed the CSBM diet (P < 0.05). The FSBMB diet had higher SIDs in most AAs compared with the FSBMA diet (P < 0.05), and higher SIDs of Lys, Ala, Pro, Ser, and Tyr compared with PSBM diet (P < 0.05). However, there was no response of UV-sterilization on the FSBMB in the SIDs of AAs. These results suggest that SIDs of AAs could be improved by the supplementation of fermented soybean products in the diet for weaning pigs but fermentation with Bacillus subtilis is more efficient in improving ileal AA digestibility than that with Aspergillus oryzae. Furthermore, probiotics supplementation in the CSBM and UV-sterilization of the FSBMB had no effects on chemical composition and ileal AA digestibility.

• Journal of Animal Science and Technology
• /
• v.44 no.6
• /
• pp.727-738
• /
• 2002

This study was conducted to investigate the effect of essential amino acid(EAA) deficient diets on short-term feeding response and the Fos expression in brain area when methionine deficiency diet fed, and thereby to know the mechanism of feed intake regulation. In all trials, experimental diets were formulated with pure amino acid mixture to level of 15% nitrogen. Rats were adapted to a 6-hr single-meal feeding per day(17:00${\sim}$21:00). Feed intake and body weight were monitored every hour after 7-day of feeding of individual EAA deficient diets in Exp. Ⅰ. In Exp. Ⅱ, Fos immuno- histochemistry was determined in various regions of brain to identify the regions that is related to suppressed feed intake following feeding methionine-deficient diet. Fos expression was examined to know the initial sensitive region in the brains of rats at 3h after feeding of the control and methionine deficient diet(-Met). Initial response to EAA deficiency diets was severely depressed in methionine deficiency diet, but the depression was low in threonine deficiency diet. However, the feed intake at 3rd day in rats was depressed in the order of His(71%), Leu(68%), Ile(66%), Thr(63%), Trp(61%), Val(55%), Phe(52%), Met(51%), Lys(44%) and Arg(24%). Fos immunoreaction in neural regions(PPC, amygdala and EPC) of pyrifrom cortex was increased in the -Met group more than in the control diet group, but those in LH, VMH and PVM were similar. Thus, based on these data, the PPC was identified as the initial response area in the -EAA diet.

• Korean Journal of Food Science and Technology
• /
• v.21 no.4
• /
• pp.498-504
• /
• 1989

Processing conditions and food components of frozen seasoned anchovy meat products were investigated. The separated anchovy meat was chopped, mixed with 12.8% emulsion curd, 0.5% table salt, 2.0% sugar, 0.4% sodium bicarbonate, 0.2% polyphosphate, 0.2% monosodium glutamate, 0.3% onion powder, 0.1% garlic powder, 0.1% ginger powder, 3.0% soybean protein, and 0.2% sodium erythorbate by remodeled stone mortar. This seasoned anchovy meat was frozen with contact freezer, Packed in a carton box and then stored at $-25{\pm}2^{\circ}C$. The major fat acids of product were linoleic, oleic, palmitic, docosahexaenoic, linolenic, palmitoleic, eicosapentaenoic acid. Amino acid composition of product were mainly consisted of Glu, Asp, Leu, Lys and Ala. The taste compounds of product were IMP 160.0 mg/100g ; free amino acids such as Glu, His, Ala, Leu 503.7 mg/100g ; total creatinine 158.3 mg/100g and small amounts of betaine, TMAO.

• Korean Journal of Food Science and Technology
• /
• v.22 no.7
• /
• pp.786-792
• /
• 1990

In order to study the possibility of savoury material production from fish flesh(Alaska pollack), as a substrate of fermentation, three strains of Aspergillus oryzae were applied to the flesh solely or with soybean. The fermented flesh were analyzed their amino acid compositions, nucleotide contents and their sensory acceptability. All strains tested were grown vigorously on both substrates. When fish flesh solely used, amino nitrogen($NH_2-N$) and soluble solid (S.S.) contents were $8{\sim}14\;and\;2{\sim}3$ times higher than those of raw flesh, respectively after 7 days fermentation. In case of fish flesh with 10% soybean used, their $NH_{2}-N$ and S.S. contents were $4{\sim}6\;and\;2.6{\sim}3$ times higher than those of raw flesh respectively. It was belived that increment of $NH_2-N$ and S.S. contents were resulted from degradation of fish flesh protein. On the other hand, the nucleotides and their related compounds of both fermented flesh and with 10% soybean were increased comparing with raw flesh. The content of free amino acids of flesh solely and with 10% soybean were $1,435{\sim}4,326mg%(DB)\;and\;5,563{\sim}6,362mg%(DB)$, which increased to between $4.6{\sim}13.9\;and\;11.6{\sim}13.2$ times, respectively after 7 days fermentation. The four amino acids- glutamic acid, histidine, aspartic acid and lysine were major in both flesh solely and flesh with 10% soybean. In sensory evaluation, fermented flesh extracts was more acceptable than anchovy extract. In review of all results, Aspergillus oryzae KFCC 32343 could be successfully used for production of savoury material from Alaska pollack.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.27 no.5
• /
• pp.509-514
• /
• 1994

A processing method for fermented paste of favorable flavor and texture using pen shell by-product was investigated. The pen shell by-product was homogenized with the addition of water and hydrolyzed with $5\%$ of Protin P(105 PU/g) at $55^{\circ}C$ for 1 hour. Flavor of the hydrolysate could be improved by thermal treatment at $100^{\circ}C$ for 40 minutes with $10\%$ of invert sugar. $2\%$ of agar-agar and $6\%$ of starch added to hydrolysate emulsified by $8\%$ of polyglycerol condensed ricinoleate(PGDR) were significantly effective for the improvement of rheological properties such as hardness, springiness and chewiness of the fermented paste. $15\%$ of table salt was finally added to the product of fermented pen shell paste. The contents of moisture, protein, lipid, carbohydrate and salinity of the product were $62.7\%,\;3.2\%,\;4.4\%,\;10.6\%\;and\;15.6\%$, respectively. The major free amino acids were glutamic acid, arginine, aspartic acid, leucine, lysine, glycine and alanine. The product was stable for the storage of 60 days at $23{\pm}3^{\circ}C$ on bacterial growth.